##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718598
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29064372569921	31.0	31.0	33.0	28.0	34.0
2	31.29372889988561	31.0	31.0	34.0	28.0	34.0
3	31.09558195263555	31.0	31.0	34.0	28.0	34.0
4	34.8771496720002	35.0	35.0	37.0	32.0	37.0
5	35.24422556144047	35.0	35.0	37.0	33.0	37.0
6	35.22489208152542	37.0	35.0	37.0	33.0	37.0
7	35.41292488985497	37.0	35.0	37.0	33.0	37.0
8	35.20234818354629	37.0	35.0	37.0	32.0	37.0
9	37.170323324028175	39.0	37.0	39.0	34.0	39.0
10	36.62258174946215	38.0	35.0	39.0	32.0	39.0
11	36.884836862891355	39.0	37.0	39.0	33.0	39.0
12	37.062434073014394	39.0	37.0	39.0	33.0	39.0
13	37.145203576965145	39.0	37.0	39.0	33.0	39.0
14	38.21395272461098	40.0	38.0	41.0	33.0	41.0
15	38.31352717374666	40.0	38.0	41.0	34.0	41.0
16	38.318286441097804	40.0	38.0	41.0	34.0	41.0
17	38.174557958691786	40.0	37.0	41.0	33.0	41.0
18	37.97297654599651	39.0	37.0	41.0	33.0	41.0
19	37.64634190465323	39.0	37.0	40.0	33.0	41.0
20	37.54728513021188	39.0	35.0	41.0	33.0	41.0
21	37.44538392814898	39.0	35.0	41.0	33.0	41.0
22	37.59800472586898	39.0	35.0	41.0	33.0	41.0
23	37.66939095293892	39.0	35.0	41.0	33.0	41.0
24	37.52887010539968	39.0	35.0	41.0	33.0	41.0
25	37.200244086401575	39.0	35.0	41.0	33.0	41.0
26	37.250778599439464	39.0	35.0	41.0	33.0	41.0
27	37.3339307930164	39.0	35.0	41.0	33.0	41.0
28	37.12540112830818	39.0	35.0	41.0	33.0	41.0
29	37.11294492887539	39.0	35.0	41.0	33.0	41.0
30	36.82035018188194	39.0	35.0	40.0	32.0	41.0
31	36.636776055597146	39.0	35.0	40.0	31.0	41.0
32	36.36672242338554	39.0	35.0	40.0	30.0	41.0
33	35.94047575974328	39.0	35.0	41.0	28.0	41.0
34	35.57497515996426	39.0	35.0	41.0	25.0	41.0
35	35.295862220601784	39.0	35.0	41.0	22.0	41.0
36	35.140815866451064	39.0	35.0	41.0	21.0	41.0
37	35.12059872139917	39.0	35.0	41.0	21.0	41.0
38	34.96978004391885	39.0	35.0	41.0	20.0	41.0
39	34.91323104155592	39.0	35.0	41.0	19.0	41.0
40	34.823130595966035	39.0	35.0	41.0	18.0	41.0
41	34.62126529714806	39.0	34.0	40.0	18.0	41.0
42	34.630355219469024	39.0	35.0	40.0	18.0	41.0
43	34.58532308745641	39.0	35.0	40.0	18.0	41.0
44	34.51334821416147	38.0	34.0	40.0	18.0	41.0
45	34.48293343427062	38.0	34.0	40.0	18.0	41.0
46	34.36997180621154	38.0	34.0	40.0	18.0	41.0
47	34.35455011007545	38.0	34.0	40.0	18.0	41.0
48	34.243131764908895	38.0	34.0	40.0	18.0	41.0
49	34.253073345597954	38.0	34.0	40.0	18.0	41.0
50	34.123848104225175	38.0	34.0	40.0	17.0	41.0
51	32.98736150114529	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	19.0
10	33.0
11	23.0
12	18.0
13	22.0
14	18.0
15	34.0
16	76.0
17	144.0
18	333.0
19	587.0
20	1125.0
21	1878.0
22	2829.0
23	4235.0
24	6875.0
25	11941.0
26	17081.0
27	18295.0
28	15709.0
29	14055.0
30	13715.0
31	16075.0
32	19342.0
33	25216.0
34	40120.0
35	50229.0
36	55385.0
37	83811.0
38	153349.0
39	165986.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.5994589464485	19.097464785596397	21.703233240281772	23.59984302767333
2	40.20829448453795	23.912952721827782	21.316647137899075	14.562105655735195
3	27.006893979665957	23.336135085263248	34.94401598668519	14.712954948385606
4	24.368701276652594	21.412249964514235	38.18797157798936	16.03107718084381
5	23.18445083342843	24.730238603502933	34.15428375809563	17.931026804973015
6	21.06281954583787	31.80011633764636	33.57148224737614	13.565581869139631
7	67.10706124982258	5.1493324501320625	22.504515737589028	5.239090562456338
8	67.74107359051932	6.423201845816437	20.095797650424853	5.739926913239391
9	61.95035332689487	6.271517593981614	22.02483168614441	9.753297392979105
10	32.103484841316	21.312333182112948	29.984915070734957	16.599266905836085
11	24.935221083276048	21.951076958188025	34.136332135630774	18.977369822905157
12	21.265297148057748	19.589673224807193	38.522511891210385	20.622517735924674
13	19.890119371331398	20.82638693678524	41.74865501991378	17.534838671969585
14	17.59509489311131	26.234000094628705	37.61240638020145	18.558498632058537
15	16.296315881758648	24.353811171197247	41.641780244309054	17.708092702735048
16	18.196404665751924	23.435773547936396	38.50456026874553	19.86326151756615
17	17.909735345770514	24.628512742868754	36.28050732120045	21.181244590160283
18	18.379121567274055	25.307891199251877	36.017912657702915	20.295074575771153
19	18.629470162733544	27.377337537816693	33.787458356410674	20.205733943039085
20	19.715752061653387	25.53333017904308	34.99759253435161	19.75332522495192
21	19.399859170217564	27.431192405211256	34.957375333635774	18.211573090935403
22	17.735228876228433	23.871483082335327	34.98270242889627	23.410585612539975
23	17.538317668571302	27.138539211074896	34.704104381030845	20.61903873932296
24	19.09509906790723	24.74777274637558	35.09194292219016	21.065185263527034
25	19.76571045285403	26.727182652887983	32.93454754953395	20.57255934472403
26	17.615690552993467	25.02525751532846	34.738894347048	22.62015758463007
27	20.027887636759356	24.399316446747697	34.152892159454936	21.41990375703801
28	17.809818563369227	24.26948029357165	35.83436079699637	22.086340346062748
29	19.020370220902368	22.649241996220418	34.03237971717149	24.29800806570572
30	18.39985638702028	23.056423758485273	35.722337106421115	22.821382748073333
31	18.86019721735936	22.61598278870801	33.434827260860736	25.088992733071898
32	18.516611512973874	23.737193813509084	33.802209302001955	23.94398537151509
33	17.113740923297865	22.99074030264487	33.16597040348011	26.72954837057715
34	16.867567123760434	22.99213190128556	33.57635284261855	26.56394813233546
35	16.447304334273127	22.47974528178481	34.06396900631508	27.008981377626988
36	17.6412959679821	25.460410410271113	30.377763367000743	26.52053025474605
37	16.804945184929544	25.428403641535326	34.69255411231315	23.07409706122199
38	17.114158402890073	26.057545386989666	30.83518184019438	25.993114369925884
39	19.011324829737905	24.614875076190028	32.14328456243964	24.23051553163243
40	19.078956523675267	23.355199986640653	33.776186407421115	23.789657082262963
41	17.616803831906015	25.91337576781455	32.708134450694274	23.761685949585164
42	18.831530285361218	26.16831663878831	31.467245942794165	23.532907133056312
43	19.700444476605835	25.351169916977224	31.43983144957264	23.5085541568443
44	20.176788691312805	24.521637967264034	31.568554323836135	23.733019017587022
45	19.665515350724604	22.855894394362355	31.616564476939818	25.86202577797322
46	21.769612495442516	24.183479497577228	29.977400438075254	24.069507568905006
47	19.02120518008678	23.512033153446016	33.66416271684586	23.802598949621345
48	18.571857979009128	24.062549575701574	31.870948708457302	25.494643736831996
49	18.636428155936976	22.90780102365996	33.643706216827766	24.812064603575294
50	18.350176315547774	21.379269076729965	35.13953559570163	25.131019012020626
51	18.46136504693862	21.230507182040583	32.666247331609604	27.64188043941119
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	966.0
1	1070.5
2	1175.0
3	11331.5
4	21488.0
5	14950.0
6	8412.0
7	8262.0
8	8112.0
9	7989.0
10	7866.0
11	7772.0
12	7678.0
13	7404.5
14	7131.0
15	6702.0
16	6273.0
17	5936.0
18	5599.0
19	5303.0
20	5007.0
21	4620.5
22	4234.0
23	4201.5
24	4169.0
25	4406.0
26	5137.5
27	5632.0
28	6263.0
29	6894.0
30	8229.5
31	9565.0
32	10668.0
33	11771.0
34	14145.0
35	16519.0
36	18088.5
37	19658.0
38	21565.0
39	23472.0
40	25851.5
41	28231.0
42	31076.5
43	33922.0
44	38634.0
45	43346.0
46	64327.0
47	85308.0
48	81830.5
49	78353.0
50	75744.0
51	73135.0
52	60476.5
53	47818.0
54	41154.5
55	34491.0
56	30178.0
57	25865.0
58	23908.0
59	21951.0
60	19743.0
61	17535.0
62	15584.0
63	13633.0
64	11756.5
65	9880.0
66	8172.0
67	6464.0
68	5443.5
69	4423.0
70	3641.0
71	2859.0
72	2498.5
73	2138.0
74	1798.5
75	1094.5
76	730.0
77	572.0
78	414.0
79	303.0
80	192.0
81	150.0
82	108.0
83	77.0
84	46.0
85	32.0
86	18.0
87	13.5
88	9.0
89	4.5
90	0.0
91	2.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	718598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.56150466986406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45996947339046	31.77791378325981
2	9.303342785715339	7.733218492677084
3	3.6370238767392897	4.534805545125213
4	2.068740931151922	3.43919943483238
5	1.3719772348338508	2.8510719126247137
6	1.0267052281720586	2.560284848114811
7	0.7795304276845845	2.2678920257359794
8	0.6771446736840654	2.2514521213995087
9	0.5409043904645014	2.0232720315216315
>10	4.046269424816481	28.36271672773713
>50	0.05612695852718472	1.5583133619786658
>100	0.023190177527087255	1.801782180405906
>500	0.0030248057644026083	0.9153483702767448
>1k	0.005377432470049082	5.020936543506423
>5k	3.360895293780676E-4	1.1324170971819882
>10k+	3.360895293780676E-4	1.7693755236220854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12667	1.7627379981575233	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	8107	1.1281690180045032	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	4801	0.6681065073935636	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	4568	0.6356822590655693	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	3694	0.5140565378695737	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	2904	0.4041202452553444	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2480	0.345116462890239	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	2271	0.31603205129989226	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2129	0.29627135060214477	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2113	0.2940447927770464	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	2046	0.2847210818844472	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1911	0.26593450023518017	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	1455	0.20247760221987818	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1204	0.1675484763386483	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	1159	0.1612862824555593	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	1088	0.15140593210668551	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	1075	0.14959685387379312	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTCTCAT	1047	0.14570037767987107	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	988	0.13748994569982104	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	841	0.11703344568173026	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.11174537084712174	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	801	0.11146705111898447	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	794	0.11049293207050395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.349591844118686E-4	0.0	0.0	0.21291459202502652	0.0
2	8.349591844118686E-4	0.0	0.0	0.9528275892780108	0.0
3	8.349591844118686E-4	0.0	0.0	1.6878699912885926	0.0
4	8.349591844118686E-4	0.0	0.0	2.588373471676793	0.0
5	8.349591844118686E-4	0.0	0.0	4.958544276493951	0.0
6	8.349591844118686E-4	0.0	0.0	7.064728819172889	0.0
7	8.349591844118686E-4	0.0	0.0	8.751346371684864	0.0
8	9.741190484805134E-4	0.0	0.0	10.944644989270774	0.0
9	9.741190484805134E-4	0.0	0.0	12.113866167175528	0.0
10	9.741190484805134E-4	0.0	0.0	14.299650152101732	0.0
11	9.741190484805134E-4	0.0	0.0	15.81036963643094	0.0
12	9.741190484805134E-4	0.0	0.0	17.714494056482206	0.0
13	9.741190484805134E-4	0.0	0.0	18.451902176181953	0.0
14	9.741190484805134E-4	0.0	0.0	18.87146916634892	0.0
15	9.741190484805134E-4	0.0	0.0	19.324852003484562	0.0
16	9.741190484805134E-4	0.0	0.0	19.89220676929243	0.0
17	9.741190484805134E-4	0.0	0.0	20.557112599812413	0.0
18	9.741190484805134E-4	0.0	0.0	21.34907138622707	0.0
19	9.741190484805134E-4	0.0	0.0	21.974177495623422	0.0
20	0.0011132789125491583	0.0	0.0	22.450382550466326	0.0
21	0.0011132789125491583	0.0	0.0	22.93772039443472	0.0
22	0.0011132789125491583	0.0	0.0	23.513981391542977	0.0
23	0.0011132789125491583	0.0	0.0	24.07215160632231	0.0
24	0.0011132789125491583	0.0	0.0	24.53736303190379	0.0
25	0.0011132789125491583	0.0	0.0	24.960130698944333	0.0
26	0.0011132789125491583	0.0	0.0	25.36717330134512	0.0
27	0.0011132789125491583	0.0	0.0	25.821669417393313	0.0
28	0.0011132789125491583	0.0	0.0	26.260997108258024	0.0
29	0.0011132789125491583	0.0	0.0	26.69086192836607	0.0
30	0.0011132789125491583	0.0	0.0	27.229271442447654	0.0
31	0.0011132789125491583	0.0	0.0	27.68780319455384	0.0
32	0.0011132789125491583	0.0	0.0	28.125600126913795	0.0
33	0.0011132789125491583	0.0	0.0	28.567015215739538	0.0
34	0.001252438776617803	0.0	0.0	28.995627597070964	0.0
35	0.0015307585047550927	0.0	0.0	29.43133713146989	0.0
36	0.0015307585047550927	0.0	0.0	29.84018881210357	0.0
37	0.0016699183688237372	0.0	0.0	30.285222057395096	0.0
38	0.001809078232892382	0.0	0.0	30.721488231250294	0.0
39	0.0019482380969610268	0.0	0.0	31.16345995953231	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	30	2.164361E-6	45.000004	41
AACGTCG	20	7.03152E-4	45.0	1
GCGATAT	50	2.1827873E-11	45.0	9
CATCGTT	25	3.8893406E-5	45.0	36
GCGTGAT	20	7.03152E-4	45.0	15
AATAGGC	20	7.03152E-4	45.0	3
GTACGAG	50	2.1827873E-11	45.0	1
CGTTTTT	8935	0.0	43.614998	1
CGTTTCT	335	0.0	40.298508	1
AAGTACG	40	3.455807E-7	39.375	1
TACCCGC	40	3.455807E-7	39.375	28
TTACCCG	40	3.455807E-7	39.375	27
CGATATG	40	3.455807E-7	39.375	10
GCCCGAA	40	3.455807E-7	39.375	25
AACGTAG	35	6.2450217E-6	38.571426	1
TCGTTGA	35	6.2450217E-6	38.571426	24
GAAGCGT	35	6.2450217E-6	38.571426	29
CGACGGT	35	6.2450217E-6	38.571426	28
TACGGTT	35	6.2450217E-6	38.571426	33
GCGTTTA	35	6.2450217E-6	38.571426	32
>>END_MODULE