FastQCFastQC Report
Sat 18 Jun 2016
SRR3550940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716609
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC151232.1103558565410148No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC119771.6713437871977606No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG106781.4900733873004663No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC40250.561673102068213No Hit
GAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT36260.5059942032544945No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT23660.3301661017374886No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.3277938178281322No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC20810.2903954597276897No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCCAGGTT19030.26555625173560476No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT18180.25369483218882266No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTA15580.2174128429869008No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC13480.18810815940073317No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8820.12307967106190405No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT8760.12224239438801356Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT207.031506E-445.030
CGACGGT453.8380676E-1045.028
ACCGCGA406.8084773E-945.030
CGCGATC253.8893304E-545.032
CGCGACA207.031506E-445.021
ACATGCG207.031506E-445.01
CGGTCTA406.8084773E-945.031
TAGGCCG207.031506E-445.01
CCGCGAT253.8893304E-545.031
GCTCGAA406.8084773E-945.042
ACGGTCT453.8380676E-1045.030
CGAACCC600.044.99999634
AAGCACG302.1643536E-644.9999961
TGAACGG850.042.352942
ACATACG850.042.3529417
CGATGAA11150.042.17488519
TAATACG1750.041.1428574
CCGATGA10950.041.0958918
TACGGCT16000.040.9218757
CGAATAT1100.040.90909214