##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716609
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.852665819156613	33.0	31.0	34.0	30.0	34.0
2	31.897196379057476	33.0	31.0	34.0	30.0	34.0
3	31.940976180874088	33.0	31.0	34.0	30.0	34.0
4	35.66090852891884	37.0	35.0	37.0	33.0	37.0
5	33.62153140694577	37.0	35.0	37.0	32.0	37.0
6	34.53001008918392	37.0	35.0	37.0	30.0	37.0
7	35.5890562356878	37.0	35.0	37.0	32.0	37.0
8	35.50460990582033	37.0	35.0	37.0	33.0	37.0
9	37.37688474467946	39.0	37.0	39.0	34.0	39.0
10	37.04803735370334	39.0	37.0	39.0	33.0	39.0
11	37.00732617089654	39.0	37.0	39.0	33.0	39.0
12	37.045038507749695	39.0	37.0	39.0	33.0	39.0
13	36.98001141487199	39.0	37.0	39.0	33.0	39.0
14	38.13979171347276	40.0	37.0	41.0	33.0	41.0
15	38.128032162587964	40.0	37.0	41.0	33.0	41.0
16	38.19681723227032	40.0	37.0	41.0	34.0	41.0
17	38.046195345020784	40.0	37.0	41.0	33.0	41.0
18	38.04057721853898	40.0	37.0	41.0	33.0	41.0
19	37.977580521595456	40.0	37.0	41.0	33.0	41.0
20	37.831241304532874	40.0	36.0	41.0	33.0	41.0
21	37.93324253532959	40.0	37.0	41.0	33.0	41.0
22	37.97244941104563	40.0	37.0	41.0	33.0	41.0
23	37.91062908782893	40.0	37.0	41.0	33.0	41.0
24	37.86979091805992	40.0	36.0	41.0	33.0	41.0
25	37.7941904162521	40.0	36.0	41.0	33.0	41.0
26	37.73105138227402	40.0	36.0	41.0	33.0	41.0
27	37.699491633512835	40.0	36.0	41.0	33.0	41.0
28	37.61872792554936	40.0	36.0	41.0	33.0	41.0
29	37.64864521656859	40.0	36.0	41.0	33.0	41.0
30	37.50842649199215	40.0	36.0	41.0	33.0	41.0
31	37.554427867916814	40.0	36.0	41.0	33.0	41.0
32	37.33640660388022	40.0	36.0	41.0	32.0	41.0
33	37.20258746401455	40.0	36.0	41.0	32.0	41.0
34	37.12109672080591	40.0	36.0	41.0	32.0	41.0
35	36.99838405601939	40.0	36.0	41.0	31.0	41.0
36	36.76835903540145	40.0	35.0	41.0	30.0	41.0
37	36.83232418236444	40.0	35.0	41.0	31.0	41.0
38	36.74445478636188	39.0	35.0	41.0	31.0	41.0
39	36.70322728293951	39.0	35.0	41.0	30.0	41.0
40	36.52790294288796	39.0	35.0	41.0	30.0	41.0
41	36.35411221461076	39.0	35.0	41.0	30.0	41.0
42	36.45534873271198	39.0	35.0	41.0	30.0	41.0
43	36.3250070819652	39.0	35.0	41.0	30.0	41.0
44	36.33369103653457	39.0	35.0	41.0	30.0	41.0
45	36.38487236414837	39.0	35.0	41.0	30.0	41.0
46	36.24492854541319	39.0	35.0	41.0	30.0	41.0
47	36.05473975347784	39.0	35.0	41.0	30.0	41.0
48	36.05119528222504	39.0	35.0	40.0	29.0	41.0
49	36.0144179043244	39.0	35.0	40.0	29.0	41.0
50	35.85021120304099	39.0	35.0	40.0	28.0	41.0
51	34.735222415571116	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	16.0
11	7.0
12	4.0
13	5.0
14	16.0
15	27.0
16	45.0
17	82.0
18	152.0
19	397.0
20	1052.0
21	2076.0
22	3141.0
23	3906.0
24	4674.0
25	5251.0
26	6092.0
27	6961.0
28	7521.0
29	8823.0
30	11076.0
31	14769.0
32	19358.0
33	26556.0
34	42276.0
35	53124.0
36	55700.0
37	82311.0
38	140088.0
39	220956.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.44499301571708	18.989155871611995	22.973336924319955	11.59251418835097
2	28.535086776749942	32.325856917789196	23.069065557368106	16.069990748092753
3	28.701565288741836	31.580262039689703	25.004151496841377	14.714021174727081
4	25.490330152147127	23.105905731019284	33.87788877895756	17.525875337876023
5	28.93516548075729	30.988726069586065	22.06377536425024	18.012333085406407
6	23.79470534140654	38.121346508346946	23.991325813658495	14.092622336588015
7	75.48523671904762	4.759359706618254	15.396680756172474	4.358722818161648
8	74.83941731125341	10.679882613810321	9.363962774679079	5.116737300257183
9	68.6389649027573	7.024472201716696	10.519125492423344	13.817437403102668
10	37.46352613489364	31.059755040754446	17.630534922112336	13.846183902239575
11	29.013311303653737	22.13354842040778	29.119784987350144	19.733355288588335
12	25.09569374652007	20.01551752768944	31.823211821230267	23.065576904560228
13	23.995512197027946	22.41319882948721	34.939416055338405	18.651872918146438
14	19.929836214727974	29.905987784133327	28.55155321800312	21.612622783135574
15	17.844598658403676	24.89530552923561	36.26998823626273	20.990107576097984
16	20.369825106857437	25.368366849983744	29.180766638431837	25.081041404726985
17	20.055846354148496	26.382867086514405	30.447705792140482	23.113580767196616
18	20.564352387424663	25.37213459501625	29.94533978780618	24.118173229752905
19	21.167749777075084	26.665587510064764	27.455139413543506	24.711523299316642
20	25.15918722762343	26.082982491149288	30.524456153913782	18.2333741273135
21	22.312586082508034	29.819469194497977	28.79073525451118	19.077209468482813
22	20.704317138076693	23.199959810719655	30.955514094855076	25.14020895634858
23	21.816081014890965	28.439637235926423	29.044848725036942	20.699433024145662
24	22.744620846235534	25.421952557112736	28.4727096645451	23.36071693210663
25	19.82503708437935	32.01606454844971	25.739280416517236	22.419617950653702
26	19.70349242055291	25.62080576716173	29.521956883042215	25.153744929243143
27	23.544778254250225	25.945390024406617	27.435044773370137	23.07478694797302
28	17.930140425252823	27.250704359001908	30.13498295444238	24.684172261302887
29	20.86367879834052	22.928821714491445	29.11992453346246	27.08757495370558
30	19.743402608675023	26.87267394074035	31.547050064958714	21.836873385625914
31	23.003757976804646	24.140361061611003	24.649285733224115	28.206595228360236
32	24.542672503415393	26.32676954936374	24.69059138246938	24.43996656475149
33	22.02065561554488	26.241088236402277	26.032048160154282	25.706207987898562
34	24.67496221789009	23.745445563759315	26.102937585210345	25.47665463314025
35	23.452538274009953	23.099067971515847	28.435729944781603	25.012663809692594
36	20.680036114533866	26.29802305022683	27.921362974788206	25.100577860451097
37	20.70180530805502	24.160734794009006	31.604543063232533	23.532916834703443
38	20.729714530518038	26.709823627668644	25.540566752580556	27.019895089232758
39	24.559418036893202	23.79637989475432	26.893745403699924	24.75045666465255
40	23.731770044752437	25.16155951153279	28.85967103399483	22.246999409719944
41	21.515219596739644	25.278917791989773	28.796875283453037	24.40898732781754
42	23.22982267875508	23.57645522174575	30.3451393995889	22.84858269991027
43	22.923798054448103	23.113999405533562	28.77315244435948	25.18905009565886
44	21.678907186485237	23.681254352094378	29.03326639771479	25.606572063705592
45	21.209474064657297	22.7405740089784	27.062875291825804	28.9870766345385
46	25.2412403416647	25.177049129999762	26.51041223317039	23.071298295165146
47	19.504918302728544	24.17371258245431	33.65615000648889	22.66521910832825
48	21.67974446315913	24.37228670027867	28.07137504552692	25.87659379103528
49	20.496393430727217	22.40608197775914	32.95255850819624	24.144966083317403
50	21.23780192545726	22.800997475610828	30.383793672700175	25.57740692623174
51	20.5859820348335	21.945161168782416	27.65817900696196	29.810677789422122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	173.0
1	506.5
2	840.0
3	2712.0
4	4584.0
5	3085.5
6	1587.0
7	1588.0
8	1589.0
9	1529.0
10	1469.0
11	1482.0
12	1495.0
13	1459.0
14	1423.0
15	1417.0
16	1411.0
17	1445.5
18	1480.0
19	1545.5
20	1611.0
21	1823.5
22	2036.0
23	2383.5
24	2731.0
25	3477.5
26	5213.0
27	6202.0
28	7074.0
29	7946.0
30	9887.5
31	11829.0
32	13400.5
33	14972.0
34	17535.5
35	20099.0
36	21848.5
37	23598.0
38	25521.5
39	27445.0
40	29686.0
41	31927.0
42	33492.0
43	35057.0
44	37877.5
45	40698.0
46	44504.0
47	48310.0
48	76817.0
49	105324.0
50	85222.5
51	65121.0
52	63214.5
53	61308.0
54	52382.5
55	43457.0
56	39689.5
57	35922.0
58	32375.0
59	28828.0
60	26407.5
61	23987.0
62	22379.0
63	20771.0
64	16707.0
65	12643.0
66	10654.0
67	8665.0
68	7350.0
69	6035.0
70	4834.0
71	3633.0
72	2967.0
73	2301.0
74	1984.0
75	1357.0
76	1047.0
77	826.5
78	606.0
79	442.0
80	278.0
81	216.5
82	155.0
83	120.5
84	86.0
85	57.0
86	28.0
87	17.0
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	716609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.291997808495267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77647925610383	22.04544950135898
2	9.998033274368396	6.057208040728605
3	3.682435973722719	3.3464502733779824
4	1.9112092231712656	2.315773823995197
5	1.2051518584913241	1.8253228728161592
6	0.9378504712010204	1.7045618650990542
7	0.7404684535662612	1.570118814088235
8	0.6787680937940119	1.6448993287747775
9	0.6185441877525157	1.6863245261871023
>10	7.286657654629855	44.9680880191223
>50	0.1260719775965647	2.4214800921014756
>100	0.030017137519852657	1.5933366982466521
>500	0.0027708126941402454	0.5882336098443524
>1k	0.004156219041210368	2.948022614473218
>5k	0.0	0.0
>10k+	0.0013854063470701227	5.284729919785955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	15123	2.1103558565410148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	11977	1.6713437871977606	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	10678	1.4900733873004663	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	4025	0.561673102068213	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	3626	0.5059942032544945	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	2366	0.3301661017374886	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2349	0.3277938178281322	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	2081	0.2903954597276897	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCCAGGTT	1903	0.26555625173560476	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT	1818	0.25369483218882266	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTA	1558	0.2174128429869008	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1348	0.18810815940073317	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	882	0.12307967106190405	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	876	0.12224239438801356	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3954611231508396E-4	0.0	0.0	0.1357783672825767	0.0
2	1.3954611231508396E-4	0.0	0.0	1.063760014177885	0.0
3	1.3954611231508396E-4	0.0	0.0	1.3505272749853825	0.0
4	1.3954611231508396E-4	0.0	0.0	2.200223552871929	0.0
5	1.3954611231508396E-4	0.0	0.0	5.2343746729388	0.0
6	1.3954611231508396E-4	0.0	0.0	6.127330245643021	0.0
7	1.3954611231508396E-4	0.0	0.0	7.073731979363921	0.0
8	1.3954611231508396E-4	0.0	0.0	8.348346169249897	0.0
9	1.3954611231508396E-4	0.0	0.0	8.78456731634685	0.0
10	2.790922246301679E-4	0.0	0.0	11.167317184126908	0.0
11	2.790922246301679E-4	0.0	0.0	12.18405015845461	0.0
12	2.790922246301679E-4	0.0	0.0	14.912874384776078	0.0
13	2.790922246301679E-4	0.0	0.0	15.369748356495663	0.0
14	4.1863833694525185E-4	0.0	0.0	15.607814024105195	0.0
15	4.1863833694525185E-4	0.0	0.0	16.105435460620786	0.0
16	6.977305615754198E-4	0.0	0.0	16.66934130048604	0.0
17	6.977305615754198E-4	0.0	0.0	17.340279008496964	0.0
18	6.977305615754198E-4	0.0	0.0	18.019031298797533	0.0
19	8.372766738905037E-4	0.0	0.0	18.778580788128533	0.0
20	9.768227862055876E-4	0.0	0.0	19.235315213735802	0.0
21	9.768227862055876E-4	0.0	0.0	19.700561952194292	0.0
22	9.768227862055876E-4	0.0	0.0	20.324891258691977	0.0
23	9.768227862055876E-4	0.0	0.0	20.841630512594733	0.0
24	9.768227862055876E-4	0.0	0.0	21.25678019673211	0.0
25	9.768227862055876E-4	0.0	0.0	21.618902358189754	0.0
26	9.768227862055876E-4	0.0	0.0	21.955208488869104	0.0
27	9.768227862055876E-4	0.0	0.0	22.35179854006857	0.0
28	9.768227862055876E-4	0.0	0.0	22.72996850444245	0.0
29	9.768227862055876E-4	0.0	0.0	23.11860442723996	0.0
30	0.0011163688985206717	0.0	0.0	23.556500127684693	0.0
31	0.0011163688985206717	0.0	0.0	23.928530063116707	0.0
32	0.0011163688985206717	0.0	0.0	24.31925917759894	0.0
33	0.0011163688985206717	0.0	0.0	24.69059138246938	0.0
34	0.0011163688985206717	0.0	0.0	25.056341742847216	0.0
35	0.0011163688985206717	0.0	0.0	25.445535850093986	0.0
36	0.0012559150108357557	0.0	0.0	25.81379804049349	0.0
37	0.0012559150108357557	0.0	0.0	26.17633884028808	0.0
38	0.0012559150108357557	0.0	0.0	26.558834734143723	0.0
39	0.0013954611231508395	0.0	0.0	26.924724640633872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTT	20	7.031506E-4	45.0	30
CGACGGT	45	3.8380676E-10	45.0	28
ACCGCGA	40	6.8084773E-9	45.0	30
CGCGATC	25	3.8893304E-5	45.0	32
CGCGACA	20	7.031506E-4	45.0	21
ACATGCG	20	7.031506E-4	45.0	1
CGGTCTA	40	6.8084773E-9	45.0	31
TAGGCCG	20	7.031506E-4	45.0	1
CCGCGAT	25	3.8893304E-5	45.0	31
GCTCGAA	40	6.8084773E-9	45.0	42
ACGGTCT	45	3.8380676E-10	45.0	30
CGAACCC	60	0.0	44.999996	34
AAGCACG	30	2.1643536E-6	44.999996	1
TGAACGG	85	0.0	42.35294	2
ACATACG	85	0.0	42.35294	17
CGATGAA	1115	0.0	42.174885	19
TAATACG	175	0.0	41.142857	4
CCGATGA	1095	0.0	41.09589	18
TACGGCT	1600	0.0	40.921875	7
CGAATAT	110	0.0	40.909092	14
>>END_MODULE