FastQCFastQC Report
Sat 18 Jun 2016
SRR3550938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568536
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC97741.7191523491916079No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC86001.5126570700887896No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG83791.4737853011946473No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC35470.6238830962331321Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC19030.3347193493463914Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT18170.31959277864550356No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGAT16610.29215388295552086No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT15960.2807210097513614No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC13870.24395992514106407No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.24167335050023214No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTA13070.22988869658209857No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT12210.21476212588121069Illumina Single End Adapter 2 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT5720.10060928419660321No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG253.888309E-545.0000041
GCGAACC253.888309E-545.00000433
AGTTACG253.888309E-545.0000041
CACCCGT253.888309E-545.00000416
AGCGTTG351.2104829E-745.01
CCGGCAC207.0302765E-445.036
ATCGTTC207.0302765E-445.023
TATAGCG207.0302765E-445.01
TTACGCC207.0302765E-445.044
ACCGTAG207.0302765E-445.01
CGCAAAT207.0302765E-445.02
TGGTCGA207.0302765E-445.01
AGTCGAG207.0302765E-445.01
TAACCGG302.1635587E-644.9999962
AATAGCG302.1635587E-644.9999961
TACGGCT11450.041.4628837
TTGCGAG556.002665E-1140.909091
ACGGCTG11800.040.423738
GCGATTA850.039.7058839
TACCAGG403.4541154E-739.3752