##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550938_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568536
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.881414721319317	33.0	31.0	34.0	30.0	34.0
2	31.932343774184925	33.0	31.0	34.0	30.0	34.0
3	31.980736488102778	33.0	31.0	34.0	30.0	34.0
4	35.682276935849266	37.0	35.0	37.0	33.0	37.0
5	33.710136209492454	37.0	35.0	37.0	32.0	37.0
6	34.615526545372674	37.0	35.0	37.0	30.0	37.0
7	35.65558909198362	37.0	35.0	37.0	32.0	37.0
8	35.60955154994583	37.0	35.0	37.0	33.0	37.0
9	37.4894799977486	39.0	37.0	39.0	35.0	39.0
10	37.14509547328577	39.0	37.0	39.0	34.0	39.0
11	37.09017195041299	39.0	37.0	39.0	33.0	39.0
12	37.13957779278709	39.0	37.0	39.0	34.0	39.0
13	37.09721108249961	39.0	37.0	39.0	33.0	39.0
14	38.24248948175665	40.0	37.0	41.0	33.0	41.0
15	38.256309187105124	40.0	37.0	41.0	33.0	41.0
16	38.30584167053625	40.0	37.0	41.0	34.0	41.0
17	38.21003946979611	40.0	37.0	41.0	33.0	41.0
18	38.204099652440654	40.0	37.0	41.0	34.0	41.0
19	38.1313689898265	40.0	37.0	41.0	34.0	41.0
20	38.073256574781546	40.0	37.0	41.0	34.0	41.0
21	38.12508618627492	40.0	37.0	41.0	34.0	41.0
22	38.18154699086777	40.0	37.0	41.0	34.0	41.0
23	38.14913567478577	40.0	37.0	41.0	34.0	41.0
24	38.09727967973884	40.0	37.0	41.0	34.0	41.0
25	37.99929995637919	40.0	37.0	41.0	34.0	41.0
26	37.993303854109506	40.0	37.0	41.0	34.0	41.0
27	37.97697419336682	40.0	37.0	41.0	34.0	41.0
28	37.91204075027791	40.0	37.0	41.0	33.0	41.0
29	37.92440936018124	40.0	37.0	41.0	33.0	41.0
30	37.82016794011285	40.0	37.0	41.0	33.0	41.0
31	37.84765608510279	40.0	37.0	41.0	33.0	41.0
32	37.7166423937974	40.0	37.0	41.0	33.0	41.0
33	37.650938550944886	40.0	37.0	41.0	33.0	41.0
34	37.55167834578637	40.0	37.0	41.0	33.0	41.0
35	37.549852603880844	40.0	37.0	41.0	33.0	41.0
36	37.459946247906906	40.0	36.0	41.0	33.0	41.0
37	37.38834656028818	40.0	36.0	41.0	33.0	41.0
38	37.36720805718547	40.0	36.0	41.0	33.0	41.0
39	37.29238605822675	40.0	36.0	41.0	32.0	41.0
40	37.21699065670424	40.0	36.0	41.0	32.0	41.0
41	37.01715986322766	40.0	35.0	41.0	31.0	41.0
42	37.10540933203878	40.0	36.0	41.0	32.0	41.0
43	37.052276373000126	39.0	35.0	41.0	32.0	41.0
44	36.96384925492845	39.0	35.0	41.0	31.0	41.0
45	36.9546519481616	39.0	35.0	41.0	31.0	41.0
46	36.8855200022514	39.0	35.0	41.0	31.0	41.0
47	36.75577096261274	39.0	35.0	41.0	31.0	41.0
48	36.700307456344014	39.0	35.0	41.0	31.0	41.0
49	36.65536747013382	39.0	35.0	41.0	31.0	41.0
50	36.54770498262203	39.0	35.0	41.0	31.0	41.0
51	35.320567211223214	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	12.0
12	6.0
13	4.0
14	11.0
15	14.0
16	22.0
17	52.0
18	104.0
19	242.0
20	492.0
21	959.0
22	1530.0
23	1969.0
24	2458.0
25	3042.0
26	3633.0
27	4508.0
28	4966.0
29	6074.0
30	7457.0
31	10035.0
32	13803.0
33	19639.0
34	31986.0
35	42197.0
36	43689.0
37	65347.0
38	114299.0
39	189865.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6120632642436	19.367990769274066	23.391130904639283	11.62881506184305
2	28.34948006810475	30.83041355340735	24.322118564171838	16.49798781431607
3	28.848832791591033	29.98385326522859	25.794496742510585	15.372817200669791
4	26.18004840502624	24.12054821506466	32.359428426695935	17.339974953213165
5	28.113435209028097	31.81803790788974	22.020593242996046	18.047933640086118
6	24.504516864367428	38.3745268549397	22.856776000112568	14.264180280580296
7	76.41257545696315	4.956766150252578	14.114321696427314	4.516336696356959
8	76.17688237860048	9.857247386269295	8.667876792322737	5.297993442807492
9	69.64062082260402	7.47252592623862	10.434695428257841	12.452157822899517
10	36.46541292020206	33.37572290936722	16.961986576047956	13.196877594382766
11	27.19862946233836	22.789058212672547	31.07718772426021	18.93512460072889
12	24.5062757679373	20.26221734419632	32.66565353821042	22.56585334965596
13	22.907608313281834	23.05007950244136	36.32452474425542	17.717787440021386
14	19.49058634809405	29.1770441977289	29.43947261035361	21.892896843823433
15	16.847481953649375	25.52749518060422	37.634732013452094	19.990290852294315
16	19.37203624748477	26.3153080895493	30.461043803734505	23.85161185923143
17	19.813696933879296	26.550297606484026	30.586277737909295	23.049727721727383
18	19.561294271602854	25.61878227588051	31.14050825277555	23.67941519974109
19	20.460797557234724	27.09344702886009	29.094551620301967	23.35120379360322
20	22.628470316743353	26.614849367498277	31.7628083357958	18.99387197996257
21	22.21882167532047	29.279236495138388	29.044422868560655	19.457518960980483
22	20.130651357169995	24.096451236157428	30.147079516512587	25.62581789015999
23	19.798746253535395	29.007485893593373	29.558022710962895	21.63574514190834
24	21.535311748068725	25.369369749672842	29.137820648120787	23.957497854137642
25	18.74252465982805	31.6702900080206	26.91386297437629	22.673322357775056
26	18.81850929404646	26.378100946993683	30.876848607651937	23.92654115130792
27	23.339595030042073	27.533524702041735	27.69376081725696	21.433119450659238
28	17.71884278216331	27.034699649626408	32.063053175172726	23.183404393037556
29	20.937284534312692	23.61627056158273	30.673519354974882	24.772925549129695
30	22.790641225885434	26.12323581971942	28.681561062096332	22.404561892298815
31	22.36727313661756	25.472441498867266	25.13578735559402	27.02449800892116
32	23.426484866393686	27.58382934414004	25.535762027382606	23.45392376208367
33	21.36804001857402	25.663986097626186	24.499943715085763	28.46803016871403
34	20.720411724147635	26.502631319740527	29.00045027931389	23.77650667679795
35	24.387725667328013	23.565614138770457	26.110571714016352	25.93608847988518
36	20.840017166898843	27.385776802172597	27.575210716647668	24.198995314280893
37	21.547448182700833	26.334656028817875	28.956125909353148	23.161769879128148
38	20.02406180083583	26.377221495208747	24.562912462887134	29.035804241068284
39	20.747674729480632	27.181216316996636	26.740083301673067	25.331025651849664
40	22.898637905075493	24.36521170163367	28.556151237564553	24.179999155726286
41	19.981144553730985	30.07865816764462	26.132909789353707	23.80728748927069
42	23.53061195773003	25.1333248905962	28.828781290894508	22.507281860779266
43	22.255230979216794	26.80111725554758	26.88061969690574	24.063032068329886
44	21.11686858879649	24.753225829147144	28.555623566493594	25.574282015562776
45	20.20646010103142	22.861173259037244	28.245704757482375	28.686661882448956
46	24.947232892903877	25.49003053456597	26.77543726342747	22.787299309102675
47	19.485661418098413	24.074640831891035	32.90451264299886	23.535185107011696
48	21.49608819846061	25.420905624270056	26.757496447014788	26.32550973025455
49	20.445670986533834	22.62987743959925	32.515970844414426	24.40848072945249
50	21.13357817271026	23.392713917852166	29.269914306218077	26.2037936032195
51	20.302144455232387	22.663472497783783	27.222550550888595	29.811832496095235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	356.0
2	573.0
3	1567.0
4	2561.0
5	1775.0
6	989.0
7	968.0
8	947.0
9	962.0
10	977.0
11	1027.0
12	1077.0
13	1031.5
14	986.0
15	1034.0
16	1082.0
17	1033.0
18	984.0
19	1025.5
20	1067.0
21	1382.5
22	1698.0
23	2196.5
24	2695.0
25	3270.0
26	4693.0
27	5541.0
28	6321.0
29	7101.0
30	8918.0
31	10735.0
32	12232.0
33	13729.0
34	15556.0
35	17383.0
36	18237.5
37	19092.0
38	20582.5
39	22073.0
40	24342.0
41	26611.0
42	28835.5
43	31060.0
44	32380.0
45	33700.0
46	38026.5
47	42353.0
48	61443.5
49	80534.0
50	69320.0
51	58106.0
52	55480.5
53	52855.0
54	43843.5
55	34832.0
56	29591.5
57	24351.0
58	21945.5
59	19540.0
60	18051.0
61	16562.0
62	13970.0
63	11378.0
64	9442.5
65	7507.0
66	6270.0
67	5033.0
68	4104.5
69	3176.0
70	2691.0
71	2206.0
72	1787.5
73	1369.0
74	1218.5
75	768.0
76	468.0
77	399.5
78	331.0
79	231.5
80	132.0
81	81.5
82	31.0
83	39.0
84	47.0
85	25.0
86	3.0
87	5.5
88	8.0
89	4.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.909712157315195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.98736215385821	21.531212809863046
2	11.372398154357128	6.802903106704086
3	4.053335756636265	3.637023172738322
4	1.9633621659507918	2.3489438897660437
5	1.1882044584289837	1.7769426667824748
6	0.804544563006059	1.44382177783465
7	0.6323161183503588	1.3238675164603058
8	0.4979421366797101	1.1914644783270902
9	0.43356650840305705	1.1671064520648855
>10	6.875186901724498	46.283416160621016
>50	0.14707138236575598	2.809817546644158
>100	0.03588541729724443	1.9115697806213365
>500	0.0017648565883890705	0.28223103167494695
>1k	0.0052945697651672115	2.7823686433141743
>5k	0.0017648565883890705	4.707310966583451
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	9774	1.7191523491916079	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	8600	1.5126570700887896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	8379	1.4737853011946473	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	3547	0.6238830962331321	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1903	0.3347193493463914	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	1817	0.31959277864550356	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGAT	1661	0.29215388295552086	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1596	0.2807210097513614	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC	1387	0.24395992514106407	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.24167335050023214	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTA	1307	0.22988869658209857	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1221	0.21476212588121069	Illumina Single End Adapter 2 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	572	0.10060928419660321	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.517807139741371E-4	0.0	0.0	0.2265467797993443	0.0
2	3.517807139741371E-4	0.0	0.0	1.4155655930319275	0.0
3	3.517807139741371E-4	0.0	0.0	1.7764926055693924	0.0
4	3.517807139741371E-4	0.0	0.0	2.476536226377925	0.0
5	3.517807139741371E-4	0.0	0.0	5.124389660461254	0.0
6	3.517807139741371E-4	0.0	0.0	5.941400368666188	0.0
7	3.517807139741371E-4	0.0	0.0	7.0375490734096	0.0
8	3.517807139741371E-4	0.0	0.0	8.655388576976655	0.0
9	3.517807139741371E-4	0.0	0.0	9.237233877889878	0.0
10	3.517807139741371E-4	0.0	0.0	11.867322385917515	0.0
11	3.517807139741371E-4	0.0	0.0	13.18755540546245	0.0
12	3.517807139741371E-4	0.0	0.0	16.19862242672408	0.0
13	3.517807139741371E-4	0.0	0.0	16.761119788368724	0.0
14	3.517807139741371E-4	0.0	0.0	17.016512586713947	0.0
15	3.517807139741371E-4	0.0	0.0	17.577778715859683	0.0
16	3.517807139741371E-4	0.0	0.0	18.2929489073691	0.0
17	3.517807139741371E-4	0.0	0.0	19.14636891947036	0.0
18	3.517807139741371E-4	0.0	0.0	20.027931388689545	0.0
19	5.276710709612056E-4	0.0	0.0	20.916705362545205	0.0
20	5.276710709612056E-4	0.0	0.0	21.498198882744454	0.0
21	5.276710709612056E-4	0.0	0.0	22.127886360758158	0.0
22	5.276710709612056E-4	0.0	0.0	22.878586404378968	0.0
23	5.276710709612056E-4	0.0	0.0	23.50598730775184	0.0
24	5.276710709612056E-4	0.0	0.0	24.003757018025244	0.0
25	5.276710709612056E-4	0.0	0.0	24.425542094080235	0.0
26	5.276710709612056E-4	0.0	0.0	24.852779771201824	0.0
27	5.276710709612056E-4	0.0	0.0	25.34615222255055	0.0
28	7.035614279482742E-4	0.0	0.0	25.756152644687408	0.0
29	8.794517849353427E-4	0.0	0.0	26.21557825713763	0.0
30	8.794517849353427E-4	0.0	0.0	26.79460931233906	0.0
31	0.0010553421419224112	0.0	0.0	27.26212588121069	0.0
32	0.0010553421419224112	0.0	0.0	27.693584926899966	0.0
33	0.0010553421419224112	0.0	0.0	28.119239590808675	0.0
34	0.0010553421419224112	0.0	0.0	28.54489425471738	0.0
35	0.0010553421419224112	0.0	0.0	28.977232752191593	1.7589035698706854E-4
36	0.0010553421419224112	0.0	0.0	29.434371790000984	1.7589035698706854E-4
37	0.0010553421419224112	0.0	0.0	29.879550283535256	1.7589035698706854E-4
38	0.0010553421419224112	0.0	0.0	30.281459749250708	1.7589035698706854E-4
39	0.0010553421419224112	0.0	0.0	30.712742904583	1.7589035698706854E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACG	25	3.888309E-5	45.000004	1
GCGAACC	25	3.888309E-5	45.000004	33
AGTTACG	25	3.888309E-5	45.000004	1
CACCCGT	25	3.888309E-5	45.000004	16
AGCGTTG	35	1.2104829E-7	45.0	1
CCGGCAC	20	7.0302765E-4	45.0	36
ATCGTTC	20	7.0302765E-4	45.0	23
TATAGCG	20	7.0302765E-4	45.0	1
TTACGCC	20	7.0302765E-4	45.0	44
ACCGTAG	20	7.0302765E-4	45.0	1
CGCAAAT	20	7.0302765E-4	45.0	2
TGGTCGA	20	7.0302765E-4	45.0	1
AGTCGAG	20	7.0302765E-4	45.0	1
TAACCGG	30	2.1635587E-6	44.999996	2
AATAGCG	30	2.1635587E-6	44.999996	1
TACGGCT	1145	0.0	41.462883	7
TTGCGAG	55	6.002665E-11	40.90909	1
ACGGCTG	1180	0.0	40.42373	8
GCGATTA	85	0.0	39.705883	9
TACCAGG	40	3.4541154E-7	39.375	2
>>END_MODULE