FastQCFastQC Report
Sat 18 Jun 2016
SRR3550935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622251
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC69411.1154662668280162No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG61250.9843294747617923No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC57890.9303319721462882TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC25050.4025706668209453TruSeq Adapter, Index 14 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC13800.22177545717082014TruSeq Adapter, Index 14 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT12860.2066690129867208No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.18722348377101844No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT10100.16231392155255678No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC9600.1542785789014401No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT9200.14785030478054675Illumina PCR Primer Index 3 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTA9130.14672535680939042No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT8280.13306527430249207Illumina PCR Primer Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG351.2107193E-745.02
GGCAATA207.0307904E-445.041
TAAACGG253.8887367E-545.02
CTAAGCG207.0307904E-445.01
ATATCGT207.0307904E-445.013
TTACGCG207.0307904E-445.01
CGCACGG253.8887367E-545.02
CGCACGA207.0307904E-445.039
TTACCGA207.0307904E-445.039
CCAGTCG207.0307904E-445.028
AACGTAG302.1638916E-644.9999961
CGCATGG1200.041.2499962
CGAATAT556.002665E-1140.90909214
CACGACG451.925946E-840.026
TACGGCT8550.040.07
TAGACGG850.039.7058832
CGTAAGG403.4548066E-739.3752
ACGGCTG8850.038.6440668
TACGTAG356.243672E-638.5714261
ACACGAC3900.038.07692326
Produced by FastQC (version 0.11.5)