##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550935_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 622251 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02135794076667 33.0 31.0 34.0 30.0 34.0 2 32.076226474525555 33.0 31.0 34.0 30.0 34.0 3 32.10739556866923 33.0 31.0 34.0 30.0 34.0 4 35.78717109333693 37.0 35.0 37.0 35.0 37.0 5 33.770798279151016 37.0 35.0 37.0 32.0 37.0 6 34.702219843760794 37.0 35.0 37.0 32.0 37.0 7 35.7423869146052 37.0 35.0 37.0 33.0 37.0 8 35.70246090404033 37.0 35.0 37.0 35.0 37.0 9 37.57114572736725 39.0 37.0 39.0 35.0 39.0 10 37.25511730796736 39.0 37.0 39.0 34.0 39.0 11 37.2231093240509 39.0 37.0 39.0 34.0 39.0 12 37.16358511275996 39.0 37.0 39.0 34.0 39.0 13 37.11127181796413 39.0 37.0 39.0 33.0 39.0 14 38.26106024739213 40.0 37.0 41.0 34.0 41.0 15 38.29214095276665 40.0 37.0 41.0 34.0 41.0 16 38.33663746623147 40.0 37.0 41.0 34.0 41.0 17 38.255139806926785 40.0 37.0 41.0 34.0 41.0 18 38.24039816729905 40.0 37.0 41.0 34.0 41.0 19 38.15963172417561 40.0 37.0 41.0 34.0 41.0 20 38.16126450580232 40.0 37.0 41.0 34.0 41.0 21 38.14569201174446 40.0 37.0 41.0 34.0 41.0 22 38.186680294607804 40.0 37.0 41.0 34.0 41.0 23 38.17000213740115 40.0 37.0 41.0 34.0 41.0 24 38.106297940863094 40.0 37.0 41.0 34.0 41.0 25 37.949296987871456 40.0 36.0 41.0 34.0 41.0 26 37.94985624771997 40.0 36.0 41.0 34.0 41.0 27 37.93773252272797 40.0 36.0 41.0 34.0 41.0 28 37.8526784207659 40.0 36.0 41.0 33.0 41.0 29 37.82900308717865 40.0 36.0 41.0 33.0 41.0 30 37.73353839527779 40.0 36.0 41.0 33.0 41.0 31 37.707889581535426 40.0 36.0 41.0 33.0 41.0 32 37.63104599269427 40.0 36.0 41.0 33.0 41.0 33 37.56841210379734 40.0 36.0 41.0 33.0 41.0 34 37.46104064115606 40.0 36.0 41.0 33.0 41.0 35 37.39226614340515 40.0 36.0 41.0 33.0 41.0 36 37.31175843831509 40.0 35.0 41.0 33.0 41.0 37 37.270212502671754 40.0 35.0 41.0 33.0 41.0 38 37.14879043987073 40.0 35.0 41.0 32.0 41.0 39 37.035950123021095 39.0 35.0 41.0 32.0 41.0 40 36.90044531868973 39.0 35.0 41.0 31.0 41.0 41 36.755728797543114 39.0 35.0 41.0 31.0 41.0 42 36.75004138201465 39.0 35.0 41.0 31.0 41.0 43 36.691833359849966 39.0 35.0 41.0 31.0 41.0 44 36.55267729581792 39.0 35.0 41.0 31.0 41.0 45 36.47757576926353 39.0 35.0 41.0 31.0 41.0 46 36.284197213021756 38.0 35.0 40.0 30.0 41.0 47 36.13216210178851 38.0 35.0 40.0 30.0 41.0 48 36.066887799296424 38.0 35.0 40.0 30.0 41.0 49 35.9943157986086 38.0 35.0 40.0 30.0 41.0 50 35.858002638806525 38.0 35.0 40.0 29.0 41.0 51 34.4307232933334 36.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 14.0 10 17.0 11 12.0 12 8.0 13 13.0 14 15.0 15 22.0 16 51.0 17 84.0 18 143.0 19 270.0 20 524.0 21 1030.0 22 1452.0 23 1995.0 24 2594.0 25 3294.0 26 4204.0 27 5066.0 28 5871.0 29 6907.0 30 8699.0 31 11522.0 32 15646.0 33 22505.0 34 38772.0 35 51018.0 36 49692.0 37 75082.0 38 126694.0 39 188894.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1701965926933 20.348862436540884 23.666012589774866 10.814928380990949 2 31.189986034574474 28.28906663066833 22.576420126283445 17.94452720847375 3 31.401315546298843 27.963635253298108 24.171274935677083 16.46377426472597 4 27.959617581972545 24.308036467599088 28.519680964755377 19.212664985672983 5 26.891238423080075 32.31348764405361 21.798277543949307 18.99699638891701 6 25.695418729740894 35.566033642372616 24.05829801800238 14.680249609884113 7 77.18750150662675 5.722771036125294 12.11102915061607 4.978698306631889 8 75.96002256324216 8.845948017761321 9.264750076737522 5.929279342258992 9 69.59088856426105 7.591148909362942 11.236141042762487 11.581821483613526 10 41.00708556514975 24.74001648852312 18.011702673037085 16.24119527329004 11 32.449284934857474 23.579070182289783 24.3045009168326 19.66714396602014 12 27.69316561966152 21.49470229859012 28.103771629133583 22.70836045261478 13 24.394496754525104 24.267216926931415 31.59753861383911 19.740747704704372 14 21.174092126810564 28.453630448163203 28.12161008981906 22.250667335207176 15 19.422869549426196 23.948374530535105 34.89540394471041 21.733351975328283 16 21.975215789126896 24.054119639823803 29.551579668011783 24.41908490303752 17 20.825518962605123 24.692447260028512 29.452423539697005 25.029610237669363 18 23.09469972728047 24.38244374054843 28.705458086849195 23.817398445321906 19 23.819487634411193 26.58091348989395 26.07404407546151 23.525554800233348 20 26.22832265436295 25.102731855794524 28.025989512270773 20.64295597757175 21 24.49718843360637 28.173196989639226 26.346763605040408 20.982850971713987 22 22.70771762520269 23.840218818451074 27.041499330655956 26.410564225690276 23 22.685379372632585 27.74009202074404 27.238847346167383 22.33568126045599 24 24.199880755515057 23.902894491129786 27.208955871505232 24.68826888184993 25 22.37071535441486 28.53896578711806 25.338006688619224 23.75231216984786 26 21.423348455848203 26.760423044719893 27.61184795203222 24.20438054739968 27 23.707796371560672 25.977780670501133 26.81634902957167 23.498073928366527 28 19.759550406507984 27.178260862577964 29.652824985415855 23.409363745498197 29 22.421498719969915 26.201163196202177 27.233544020017646 24.143794063810265 30 22.977223017721144 25.75600521333031 28.834666396679154 22.43210537226939 31 24.553757245870237 25.447126641821388 25.7674153998949 24.231700712413478 32 25.347809806653586 27.481675401084125 25.66777715102105 21.502737641241236 33 23.243996393738218 24.557132089783703 25.28754473677021 26.911326779707867 34 24.580434583471945 25.475732461659362 27.646239218578998 22.2975937362897 35 22.29775444314272 24.60775474848574 28.503770986306172 24.59071982206537 36 22.13302991879483 27.701683082871703 26.81667044327771 23.348616555055756 37 24.26287784189981 24.138169323954482 29.9424187345621 21.656534099583606 38 22.163885634575113 24.732463266431072 27.660381421644963 25.44326967734885 39 26.78485048637929 23.3280460778689 25.315989849755162 24.57111358599665 40 23.9591418896876 22.695503904372995 29.39006928072434 23.955284925215064 41 21.82897255287657 25.005343502862992 26.726031778173116 26.439652166087317 42 22.28602284287209 24.46520776985493 29.950614784066236 23.298154603206743 43 23.78943545289602 23.259424251628364 27.04021367583178 25.910926619643842 44 23.29236915649794 23.840700939010144 28.024543150593573 24.842386753898346 45 22.900083728270424 22.26207752177176 27.499835275475654 27.338003474482164 46 25.374326437402274 24.288430231530363 26.864561085478368 23.472682245589 47 20.885301911929428 24.028888663899295 31.821563966952244 23.264245457219033 48 23.137769163890457 23.540500537564423 28.254996777827596 25.06673352071752 49 22.411213481376485 21.94034240202105 31.04727834909064 24.601165767511823 50 22.369911820149746 22.144600812212435 29.386855143663894 26.09863222397393 51 21.699603536193592 22.29630808146552 26.683283755269176 29.320804627071713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 199.0 1 582.0 2 965.0 3 1742.5 4 2520.0 5 1807.5 6 1095.0 7 1084.5 8 1074.0 9 1115.0 10 1156.0 11 1183.0 12 1210.0 13 1175.0 14 1140.0 15 1137.5 16 1135.0 17 1106.5 18 1078.0 19 1309.5 20 1541.0 21 1683.5 22 1826.0 23 1816.0 24 1806.0 25 2345.5 26 3274.5 27 3664.0 28 4316.0 29 4968.0 30 6231.5 31 7495.0 32 8553.0 33 9611.0 34 11057.0 35 12503.0 36 13059.0 37 13615.0 38 16255.5 39 18896.0 40 20715.0 41 22534.0 42 26497.5 43 30461.0 44 35584.0 45 40707.0 46 56980.5 47 73254.0 48 67044.5 49 60835.0 50 60970.5 51 61106.0 52 53955.5 53 46805.0 54 42784.0 55 38763.0 56 35817.5 57 32872.0 58 30912.5 59 28953.0 60 27896.0 61 26839.0 62 24138.0 63 21437.0 64 18212.0 65 14987.0 66 13050.5 67 11114.0 68 9149.0 69 7184.0 70 6274.0 71 5364.0 72 4350.0 73 3336.0 74 2893.5 75 1930.5 76 1410.0 77 1120.0 78 830.0 79 604.0 80 378.0 81 277.5 82 177.0 83 104.0 84 31.0 85 26.5 86 22.0 87 15.0 88 8.0 89 8.0 90 8.0 91 4.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 622251.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.131369265445294 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01577987922303 20.979182766006716 2 11.06436648244609 6.446402913767062 3 4.081190089984232 3.5667196666141967 4 1.9743892772860778 2.300666524414256 5 1.1751713856915933 1.7117175793383417 6 0.7610603591981836 1.3302438214256833 7 0.6137626526039531 1.2515822532041485 8 0.47202537832944186 1.1000596479021192 9 0.42603090101235896 1.116977714629326 >10 7.1360668114187344 48.86008858494479 >50 0.22745402168433668 4.056756932165308 >100 0.04493603843031915 2.282592419325222 >500 0.0033285954392829006 0.7626418597278194 >1k 0.0027738295327357503 1.1871937066244036 >5k 0.0016642977196414503 3.0471736099105815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC 6941 1.1154662668280162 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG 6125 0.9843294747617923 No Hit GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC 5789 0.9303319721462882 TruSeq Adapter, Index 14 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC 2505 0.4025706668209453 TruSeq Adapter, Index 14 (96% over 25bp) CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC 1380 0.22177545717082014 TruSeq Adapter, Index 14 (96% over 25bp) GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT 1286 0.2066690129867208 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 0.18722348377101844 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT 1010 0.16231392155255678 No Hit GAATGATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC 960 0.1542785789014401 No Hit GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT 920 0.14785030478054675 Illumina PCR Primer Index 3 (95% over 21bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTA 913 0.14672535680939042 No Hit CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT 828 0.13306527430249207 Illumina PCR Primer Index 3 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6070685302233342E-4 0.0 0.0 0.1481717184865914 0.0 2 1.6070685302233342E-4 0.0 0.0 0.9992752120928693 0.0 3 1.6070685302233342E-4 0.0 0.0 1.3369203102927918 0.0 4 1.6070685302233342E-4 0.0 0.0 1.8735204925343631 0.0 5 1.6070685302233342E-4 0.0 0.0 3.903890873618524 0.0 6 1.6070685302233342E-4 0.0 0.0 4.730888339271451 0.0 7 1.6070685302233342E-4 0.0 0.0 5.726628000597829 0.0 8 1.6070685302233342E-4 0.0 0.0 7.243379279422612 0.0 9 1.6070685302233342E-4 0.0 0.0 7.831405654631331 0.0 10 1.6070685302233342E-4 0.0 0.0 9.903881231207343 0.0 11 1.6070685302233342E-4 0.0 0.0 11.232766198849017 0.0 12 1.6070685302233342E-4 0.0 0.0 13.476715987599858 0.0 13 1.6070685302233342E-4 0.0 0.0 13.97394299085096 0.0 14 1.6070685302233342E-4 0.0 0.0 14.186879571105552 0.0 15 1.6070685302233342E-4 0.0 0.0 14.68057102359016 0.0 16 1.6070685302233342E-4 0.0 0.0 15.362289494110897 0.0 17 1.6070685302233342E-4 0.0 0.0 16.146056816300817 0.0 18 1.6070685302233342E-4 0.0 0.0 16.970804386011434 0.0 19 1.6070685302233342E-4 0.0 0.0 17.63468439584669 0.0 20 1.6070685302233342E-4 0.0 0.0 18.142518051397264 0.0 21 1.6070685302233342E-4 0.0 0.0 18.727330289545538 0.0 22 1.6070685302233342E-4 0.0 0.0 19.35569408486286 0.0 23 1.6070685302233342E-4 0.0 0.0 19.947738131397138 0.0 24 1.6070685302233342E-4 0.0 0.0 20.446893616884505 0.0 25 1.6070685302233342E-4 0.0 0.0 20.87340960480578 0.0 26 1.6070685302233342E-4 0.0 0.0 21.257016862970087 0.0 27 1.6070685302233342E-4 0.0 0.0 21.69301455521968 0.0 28 1.6070685302233342E-4 0.0 0.0 22.12161973223024 0.0 29 1.6070685302233342E-4 0.0 0.0 22.53752906785204 0.0 30 3.2141370604466684E-4 0.0 0.0 23.042470000048212 0.0 31 3.2141370604466684E-4 0.0 0.0 23.47911051970989 0.0 32 3.2141370604466684E-4 0.0 0.0 23.90257307742374 0.0 33 3.2141370604466684E-4 0.0 0.0 24.347731060295605 0.0 34 3.2141370604466684E-4 0.0 0.0 24.772479272833632 0.0 35 3.2141370604466684E-4 0.0 0.0 25.198834553901882 0.0 36 3.2141370604466684E-4 0.0 0.0 25.62776114461849 0.0 37 3.2141370604466684E-4 0.0 0.0 26.05138440918536 0.0 38 8.035342651116672E-4 0.0 0.0 26.463597487187645 0.0 39 8.035342651116672E-4 0.0 0.0 26.88047106392758 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGG 35 1.2107193E-7 45.0 2 GGCAATA 20 7.0307904E-4 45.0 41 TAAACGG 25 3.8887367E-5 45.0 2 CTAAGCG 20 7.0307904E-4 45.0 1 ATATCGT 20 7.0307904E-4 45.0 13 TTACGCG 20 7.0307904E-4 45.0 1 CGCACGG 25 3.8887367E-5 45.0 2 CGCACGA 20 7.0307904E-4 45.0 39 TTACCGA 20 7.0307904E-4 45.0 39 CCAGTCG 20 7.0307904E-4 45.0 28 AACGTAG 30 2.1638916E-6 44.999996 1 CGCATGG 120 0.0 41.249996 2 CGAATAT 55 6.002665E-11 40.909092 14 CACGACG 45 1.925946E-8 40.0 26 TACGGCT 855 0.0 40.0 7 TAGACGG 85 0.0 39.705883 2 CGTAAGG 40 3.4548066E-7 39.375 2 ACGGCTG 885 0.0 38.644066 8 TACGTAG 35 6.243672E-6 38.571426 1 ACACGAC 390 0.0 38.076923 26 >>END_MODULE