SRR3550933_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129849
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	23219	2.055053374388967	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	15801	1.398505464004482	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	14242	1.260522423792914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10629	0.9407451792230643	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	6642	0.587866166186809	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	6270	0.5549414125250366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	3304	0.29242845725402244	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	2962	0.26215892566174775	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	2958	0.2618048960524813	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	2859	0.2530426632231387	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTT	2403	0.21268328776677237	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	2291	0.202770458707314	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2157	0.19091046679689055	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTA	1942	0.17188137529882314	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1761	0.15586153547951984	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1742	0.15417989483550457	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	1601	0.141700351108865	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1304	0.11541365262083694	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1168	0.10337664590578033	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAT	20	7.033235E-4	45.0	38
CCGATCG	35	1.2119017E-7	45.0	40
TAGCGAT	25	3.8907645E-5	45.0	24
ATCGTAA	35	1.2119017E-7	45.0	14
TCATACG	25	3.8907645E-5	45.0	1
CACGTTA	20	7.033235E-4	45.0	41
CGCAATC	20	7.033235E-4	45.0	18
TTACCGG	40	6.8157533E-9	45.0	2
TCGACAA	20	7.033235E-4	45.0	18
CCGTTGA	20	7.033235E-4	45.0	26
TAATCCG	45	3.8562575E-10	45.0	4
CGTTTTT	7060	0.0	43.310905	1
TACGGCT	1840	0.0	41.69837	7
TAGTGCG	65	0.0	41.53846	1
CGAATAT	205	0.0	40.609753	14
ACGGCTG	1925	0.0	40.090904	8
TAGCACG	45	1.9283107E-8	40.0	1
GTATGCG	45	1.9283107E-8	40.0	1
TCGTAAA	40	3.4581535E-7	39.375	15
CGCCGAT	40	3.4581535E-7	39.375	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content