##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129849
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744886263562652	31.0	31.0	34.0	30.0	34.0
2	31.809216983862445	33.0	31.0	34.0	30.0	34.0
3	31.783518859599823	33.0	31.0	34.0	30.0	34.0
4	35.566040240775536	37.0	35.0	37.0	33.0	37.0
5	33.550078815841765	37.0	35.0	37.0	32.0	37.0
6	34.4803394081864	37.0	35.0	37.0	30.0	37.0
7	35.566836807396385	37.0	35.0	37.0	32.0	37.0
8	35.514558140070044	37.0	35.0	37.0	33.0	37.0
9	37.392062125115835	39.0	37.0	39.0	34.0	39.0
10	36.893810588848595	39.0	37.0	39.0	32.0	39.0
11	36.969282620952	39.0	37.0	39.0	33.0	39.0
12	37.04074703787851	39.0	37.0	39.0	33.0	39.0
13	37.028325023963376	39.0	37.0	39.0	33.0	39.0
14	38.17500834182267	40.0	37.0	41.0	33.0	41.0
15	38.17773259966597	40.0	37.0	41.0	33.0	41.0
16	38.224329976837616	40.0	37.0	41.0	34.0	41.0
17	38.13106441657248	40.0	37.0	41.0	33.0	41.0
18	38.04081784380037	40.0	37.0	41.0	33.0	41.0
19	37.868167339175415	39.0	37.0	41.0	33.0	41.0
20	37.70368164241416	39.0	36.0	41.0	33.0	41.0
21	37.75588242322647	39.0	36.0	41.0	33.0	41.0
22	37.81753313938411	39.0	36.0	41.0	33.0	41.0
23	37.791466824327856	40.0	36.0	41.0	33.0	41.0
24	37.73953687616664	40.0	36.0	41.0	33.0	41.0
25	37.59874107070945	39.0	35.0	41.0	33.0	41.0
26	37.62140958659077	39.0	36.0	41.0	33.0	41.0
27	37.59495649418639	40.0	36.0	41.0	33.0	41.0
28	37.48205910701341	39.0	35.0	41.0	33.0	41.0
29	37.473186239931174	40.0	35.0	41.0	33.0	41.0
30	37.29941877188899	39.0	35.0	41.0	33.0	41.0
31	37.21385955114356	39.0	35.0	41.0	32.0	41.0
32	36.98544053231892	40.0	35.0	41.0	31.0	41.0
33	36.62859550258486	39.0	35.0	41.0	30.0	41.0
34	36.40729601920257	39.0	35.0	41.0	30.0	41.0
35	36.2268931512087	39.0	35.0	41.0	29.0	41.0
36	35.934469119324795	39.0	35.0	41.0	27.0	41.0
37	35.98781076055296	39.0	35.0	41.0	28.0	41.0
38	35.83871473090652	39.0	35.0	41.0	27.0	41.0
39	35.76151060893978	39.0	35.0	41.0	26.0	41.0
40	35.681469824728794	39.0	35.0	41.0	26.0	41.0
41	35.5258463741615	39.0	35.0	41.0	25.0	41.0
42	35.58727316659129	39.0	35.0	41.0	26.0	41.0
43	35.45292335524481	39.0	35.0	41.0	25.0	41.0
44	35.45541483862003	39.0	35.0	40.0	25.0	41.0
45	35.47029027772738	39.0	35.0	40.0	25.0	41.0
46	35.32210675939882	39.0	35.0	40.0	24.0	41.0
47	35.18435029813718	38.0	35.0	40.0	24.0	41.0
48	35.136974055825156	38.0	34.0	40.0	24.0	41.0
49	35.05478873725604	38.0	34.0	40.0	24.0	41.0
50	34.89432304670801	38.0	34.0	40.0	23.0	41.0
51	33.632102165864644	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	10.0
11	17.0
12	10.0
13	23.0
14	17.0
15	43.0
16	65.0
17	145.0
18	402.0
19	941.0
20	1894.0
21	3290.0
22	4684.0
23	6182.0
24	8697.0
25	12956.0
26	17404.0
27	18714.0
28	17617.0
29	17641.0
30	20015.0
31	24678.0
32	31404.0
33	41892.0
34	64461.0
35	81391.0
36	89846.0
37	133303.0
38	224213.0
39	307757.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47125235319056	18.86048489665433	21.901422225447824	14.766840524707284
2	33.43544137313924	29.19106889504704	22.692589894755848	14.680899837057874
3	26.81915902036467	28.872265231902674	30.305731119822205	14.002844627910454
4	24.27881955907382	22.06108957922696	37.02662922213499	16.633461639564224
5	25.441364288502267	30.418755072580495	27.067245269058077	17.072635369859157
6	22.114194020616914	35.690875506372976	28.97157053730189	13.223359935708224
7	70.96479264043248	5.111302483783231	19.454369566198668	4.469535309585617
8	70.96470413303017	8.962436573382815	14.487511162996118	5.585348130590901
9	65.43113283279448	6.120817914606288	15.802111609604468	12.645937642994772
10	36.77836595863695	20.727371533718223	25.698920829243555	16.795341678401275
11	28.282894439876475	23.431892226306346	28.31484561211277	19.970367721704406
12	22.3898945788331	20.225357547778508	34.32210852954687	23.062639343841525
13	21.89920954038991	22.675153936499477	38.28007105374258	17.14556546936803
14	19.850617206370057	28.033038043136738	32.05534544881662	20.060999301676596
15	17.993289368756358	24.939350302562556	38.580288162400464	18.487072166280626
16	19.969040110669656	25.079546027832038	31.681047644419742	23.270366217078564
17	19.7767135254357	26.746759965269696	31.97506923491546	21.501457274379142
18	20.226862173617892	25.59147284283121	32.409198043278344	21.77246694027255
19	19.853272428439553	27.732112875260324	29.938867937219932	22.475746759080195
20	21.276648472494998	26.51938444871837	33.57696470944347	18.62700236934316
21	20.358207158655713	29.45827274264083	31.812392629457563	18.37112746924589
22	19.090515635275157	23.830441058937964	32.471861284118496	24.607182021668383
23	19.49977386358708	27.631302944021723	32.065789322289966	20.803133870101227
24	21.475878635109645	25.080696624062153	30.843767618504774	22.599657122323425
25	19.109279204566274	30.700120104544943	28.983342021809992	21.207258669078787
26	19.584386940201746	24.802960395592685	31.89904137632551	23.713611287880063
27	22.512654345846215	25.75875183320957	29.173809951595302	22.554783869348913
28	18.690108147194888	25.692371281472127	33.30834474341262	22.30917582792037
29	24.007898400582732	22.906423778752735	29.873814996517233	23.211862824147296
30	20.890667690992338	26.306258623940014	30.29245500947472	22.510618675592934
31	22.657009919024578	23.8519483577009	29.894171699050048	23.596870024224476
32	26.62276109462415	25.712550969200308	27.723704672040245	19.940983264135294
33	22.07949911890881	26.641701678719897	27.697949017966117	23.580850184405172
34	26.639400486259667	23.975770213541807	28.939707872467913	20.44512142773061
35	25.307718111004213	23.897264147686993	27.212839945868872	23.58217779543992
36	24.013120337319414	25.256737847269857	30.67073564697583	20.059406168434897
37	23.323736180675468	24.3640521875047	30.89368579341133	21.418525838408495
38	21.770254255214635	26.923420740293615	29.00290215772196	22.30342284676979
39	26.46663403693768	24.74197879539655	28.920059229153633	19.871327938512138
40	24.072508804273845	25.416316693646674	29.726273156855477	20.784901345224007
41	22.665064092635387	25.31559526981039	29.516068076353562	22.503272561200657
42	23.25204518479903	23.685288919138753	31.705298672654486	21.35736722340773
43	23.155483608871627	23.375778533237625	30.619047324022947	22.8496905338678
44	22.32369104190029	23.84274358785997	29.3402038679505	24.493361502289243
45	22.139153108070193	22.8518147115234	28.553461568758305	26.455570611648106
46	26.28660998062573	24.96643356767143	27.26275812077543	21.484198330927406
47	20.57912163483793	23.70201681817659	33.83452125018476	21.884340296800723
48	22.117822824111894	23.80247271980592	29.586431461195257	24.49327299488693
49	21.491544445319686	21.82751854451347	34.138721191946885	22.542215818219958
50	21.799638712783747	21.87796776383393	32.17429939753011	24.148094125852214
51	21.43950209275753	21.540577546203078	28.852616588588386	28.167303772451007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	356.0
1	712.0
2	1068.0
3	9855.0
4	18642.0
5	12805.5
6	6969.0
7	6835.0
8	6701.0
9	6724.5
10	6748.0
11	6595.5
12	6443.0
13	6388.5
14	6334.0
15	6090.0
16	5846.0
17	5548.0
18	5250.0
19	5282.0
20	5314.0
21	5140.5
22	4967.0
23	5223.0
24	5479.0
25	5683.5
26	7103.0
27	8318.0
28	9938.5
29	11559.0
30	13058.5
31	14558.0
32	17196.5
33	19835.0
34	23322.0
35	26809.0
36	29168.5
37	31528.0
38	34533.5
39	37539.0
40	42049.0
41	46559.0
42	50082.0
43	53605.0
44	58307.5
45	63010.0
46	71421.5
47	79833.0
48	125963.5
49	172094.0
50	144676.0
51	117258.0
52	112290.5
53	107323.0
54	90161.5
55	73000.0
56	62405.5
57	51811.0
58	45238.5
59	38666.0
60	34216.0
61	29766.0
62	25882.0
63	21998.0
64	18118.0
65	14238.0
66	11558.5
67	8879.0
68	7606.0
69	6333.0
70	5127.5
71	3922.0
72	3161.0
73	2400.0
74	1900.5
75	1115.0
76	829.0
77	599.5
78	370.0
79	271.0
80	172.0
81	169.0
82	166.0
83	99.0
84	32.0
85	25.5
86	19.0
87	11.5
88	4.0
89	3.0
90	2.0
91	3.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1129849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.55687260700572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64451768465533	26.541256061246756
2	10.282304167043378	7.312131587480911
3	3.931205580513872	4.193441280548452
4	2.02231074098962	2.8762818155658914
5	1.3098320898329758	2.32867663773796
6	0.9492912666298067	2.0252297180699483
7	0.752808994237966	1.873727345386923
8	0.5983676631144311	1.7020866215609238
9	0.5190512260541218	1.6610254489184888
>10	4.799878945419131	33.96793518328133
>50	0.1486981752871845	3.369149067417284
>100	0.034486337369214796	2.1462514591365127
>500	0.002249108954364789	0.5551784011100166
>1k	0.003498613929011894	2.6231468596620995
>5k	4.99801989858842E-4	1.1473213052388818
>10k+	9.99603979717684E-4	5.677161207637655
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	23219	2.055053374388967	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	15801	1.398505464004482	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	14242	1.260522423792914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10629	0.9407451792230643	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	6642	0.587866166186809	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	6270	0.5549414125250366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	3304	0.29242845725402244	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	2962	0.26215892566174775	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	2958	0.2618048960524813	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	2859	0.2530426632231387	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTT	2403	0.21268328776677237	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	2291	0.202770458707314	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2157	0.19091046679689055	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTA	1942	0.17188137529882314	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1761	0.15586153547951984	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1742	0.15417989483550457	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	1601	0.141700351108865	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1304	0.11541365262083694	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1168	0.10337664590578033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6552220694977825E-4	0.0	0.0	0.16781003479225984	0.0
2	2.6552220694977825E-4	0.0	0.0	1.0757189677558683	0.0
3	2.6552220694977825E-4	0.0	0.0	1.5665810210036917	0.0
4	2.6552220694977825E-4	0.0	0.0	2.6107028461325363	0.0
5	2.6552220694977825E-4	0.0	0.0	5.813785735970028	0.0
6	2.6552220694977825E-4	0.0	0.0	7.225036265908099	0.0
7	2.6552220694977825E-4	0.0	0.0	8.457236320959703	0.0
8	2.6552220694977825E-4	0.0	0.0	10.070991787398139	0.0
9	2.6552220694977825E-4	0.0	0.0	10.695234495937068	0.0
10	2.6552220694977825E-4	0.0	0.0	13.126886867183137	0.0
11	2.6552220694977825E-4	0.0	0.0	14.44821387636755	0.0
12	2.6552220694977825E-4	0.0	0.0	17.033338083230593	0.0
13	2.6552220694977825E-4	0.0	0.0	17.627753797188827	0.0
14	2.6552220694977825E-4	0.0	0.0	17.91363270667142	0.0
15	2.6552220694977825E-4	0.0	0.0	18.427152654912295	0.0
16	2.6552220694977825E-4	0.0	0.0	19.09193175371222	0.0
17	2.6552220694977825E-4	0.0	0.0	19.892127178056537	0.0
18	2.6552220694977825E-4	0.0	0.0	20.756667483885014	0.0
19	3.54029609266371E-4	0.0	0.0	21.556508878620065	0.0
20	3.54029609266371E-4	0.0	0.0	22.105520295189887	0.0
21	3.54029609266371E-4	0.0	0.0	22.73533897007476	0.0
22	3.54029609266371E-4	0.0	0.0	23.471189512934913	0.0
23	3.54029609266371E-4	0.0	0.0	24.190666186366496	0.0
24	3.54029609266371E-4	0.0	0.0	24.754458339123193	0.0
25	3.54029609266371E-4	0.0	0.0	25.277006042400355	0.0
26	3.54029609266371E-4	0.0	0.0	25.75299885205899	0.0
27	3.54029609266371E-4	0.0	0.0	26.270855663013375	0.0
28	4.4253701158296376E-4	0.0	0.0	26.768621293641893	0.0
29	4.4253701158296376E-4	0.0	0.0	27.289487356275043	0.0
30	4.4253701158296376E-4	0.0	0.0	27.893550377085788	0.0
31	4.4253701158296376E-4	0.0	0.0	28.445571045334376	0.0
32	4.4253701158296376E-4	0.0	0.0	28.98705933270729	0.0
33	4.4253701158296376E-4	0.0	0.0	29.500313758741214	0.0
34	4.4253701158296376E-4	0.0	0.0	30.01108997751027	0.0
35	4.4253701158296376E-4	0.0	0.0	30.5110682931967	0.0
36	5.310444138995565E-4	0.0	0.0	30.990601398948	0.0
37	6.195518162161492E-4	0.0	0.0	31.49695224760123	0.0
38	7.08059218532742E-4	0.0	0.0	32.00861354039345	0.0
39	7.08059218532742E-4	0.0	0.0	32.52169095162274	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAT	20	7.033235E-4	45.0	38
CCGATCG	35	1.2119017E-7	45.0	40
TAGCGAT	25	3.8907645E-5	45.0	24
ATCGTAA	35	1.2119017E-7	45.0	14
TCATACG	25	3.8907645E-5	45.0	1
CACGTTA	20	7.033235E-4	45.0	41
CGCAATC	20	7.033235E-4	45.0	18
TTACCGG	40	6.8157533E-9	45.0	2
TCGACAA	20	7.033235E-4	45.0	18
CCGTTGA	20	7.033235E-4	45.0	26
TAATCCG	45	3.8562575E-10	45.0	4
CGTTTTT	7060	0.0	43.310905	1
TACGGCT	1840	0.0	41.69837	7
TAGTGCG	65	0.0	41.53846	1
CGAATAT	205	0.0	40.609753	14
ACGGCTG	1925	0.0	40.090904	8
TAGCACG	45	1.9283107E-8	40.0	1
GTATGCG	45	1.9283107E-8	40.0	1
TCGTAAA	40	3.4581535E-7	39.375	15
CGCCGAT	40	3.4581535E-7	39.375	38
>>END_MODULE