FastQCFastQC Report
Sat 18 Jun 2016
SRR3550930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445419
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC77741.7453229431164812No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG67641.5185701552919835No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC60611.3607412346577041No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC24790.5565546148682476No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT14100.31655587211142766No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC12300.27614448418230925No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT12260.2752464533394399No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCGGTT11720.26312303696070444No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTA9710.21799698710652216No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC9520.213731340602893No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.18881098471326996No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT7320.1643396442450816Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7070.1587269514771485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGA207.028627E-445.00000441
GGTCGAC207.028627E-445.00000412
GTTAGCG302.1624965E-645.0000041
GTCTACG207.028627E-445.0000041
CGATTGA453.8380676E-1045.00000410
GTTAACG406.7993824E-945.0000041
GTCACCG207.028627E-445.00000418
CATCGAA207.028627E-445.00000430
TATACGG207.028627E-445.0000042
TCGACGC207.028627E-445.00000414
CCGTTTG207.028627E-445.0000045
CGGGTCA351.2096825E-745.06
TACGGCT8550.043.1578947
ACGGCTG8900.041.7134868
TTAACGG603.6379788E-1241.2500042
TAATACG1100.040.9090924
ACGGGGT1050.040.7142875
CGCATGG501.0786607E-940.5000042
GCATAAG403.4518598E-739.3750041
ACAACGA800.039.37500413