##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445419
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.940238292484157	33.0	31.0	34.0	30.0	34.0
2	31.992290405213968	33.0	31.0	34.0	30.0	34.0
3	32.02129455636154	33.0	31.0	34.0	30.0	34.0
4	35.73548277015574	37.0	35.0	37.0	35.0	37.0
5	33.78194015073448	37.0	35.0	37.0	32.0	37.0
6	34.65908055112153	37.0	35.0	37.0	31.0	37.0
7	35.675763719104935	37.0	35.0	37.0	32.0	37.0
8	35.59496563909487	37.0	35.0	37.0	33.0	37.0
9	37.46190665418404	39.0	37.0	39.0	35.0	39.0
10	37.20432895767805	39.0	37.0	39.0	34.0	39.0
11	37.157734627395776	39.0	37.0	39.0	34.0	39.0
12	37.04172924819103	39.0	37.0	39.0	33.0	39.0
13	36.92451601750262	39.0	37.0	39.0	33.0	39.0
14	38.02127210559047	40.0	37.0	41.0	33.0	41.0
15	38.0467896519906	40.0	37.0	41.0	33.0	41.0
16	38.15719580889006	40.0	37.0	41.0	34.0	41.0
17	38.02865616419596	40.0	37.0	41.0	33.0	41.0
18	38.03083164391281	40.0	37.0	41.0	33.0	41.0
19	37.97056479404785	40.0	37.0	41.0	34.0	41.0
20	37.893969498382425	40.0	36.0	41.0	33.0	41.0
21	37.90772059566386	40.0	36.0	41.0	33.0	41.0
22	37.954281249789524	40.0	36.0	41.0	33.0	41.0
23	37.92990195748273	40.0	36.0	41.0	34.0	41.0
24	37.87729306563034	40.0	36.0	41.0	33.0	41.0
25	37.741443449875284	40.0	36.0	41.0	33.0	41.0
26	37.73755497632566	40.0	36.0	41.0	33.0	41.0
27	37.71284565768411	40.0	36.0	41.0	33.0	41.0
28	37.6080566837068	40.0	36.0	41.0	33.0	41.0
29	37.620397872564936	40.0	36.0	41.0	33.0	41.0
30	37.49961272419901	40.0	36.0	41.0	33.0	41.0
31	37.466396808398386	40.0	36.0	41.0	33.0	41.0
32	37.379415786035175	40.0	35.0	41.0	33.0	41.0
33	37.281914332347746	40.0	35.0	41.0	33.0	41.0
34	37.179231240696964	40.0	35.0	41.0	32.0	41.0
35	37.15158760627634	40.0	35.0	41.0	32.0	41.0
36	36.974920243635765	39.0	35.0	41.0	31.0	41.0
37	36.97875932548904	39.0	35.0	41.0	32.0	41.0
38	36.859020383055054	39.0	35.0	41.0	31.0	41.0
39	36.7909316845487	39.0	35.0	41.0	31.0	41.0
40	36.726233052474186	39.0	35.0	41.0	31.0	41.0
41	36.48395331137648	39.0	35.0	41.0	30.0	41.0
42	36.55653665425139	39.0	35.0	41.0	31.0	41.0
43	36.41947694193557	39.0	35.0	41.0	31.0	41.0
44	36.407340055094195	39.0	35.0	41.0	31.0	41.0
45	36.38978130703899	39.0	35.0	41.0	31.0	41.0
46	36.248689436238685	39.0	35.0	40.0	30.0	41.0
47	36.1049730703001	38.0	35.0	40.0	30.0	41.0
48	36.03368289183892	38.0	35.0	40.0	30.0	41.0
49	35.99701629252457	38.0	35.0	40.0	30.0	41.0
50	35.81560508195654	38.0	35.0	40.0	29.0	41.0
51	34.51090546204809	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	9.0
11	10.0
12	7.0
13	5.0
14	8.0
15	16.0
16	43.0
17	55.0
18	133.0
19	252.0
20	544.0
21	1055.0
22	1481.0
23	1812.0
24	2148.0
25	2844.0
26	3243.0
27	3907.0
28	4337.0
29	5230.0
30	6582.0
31	8793.0
32	11843.0
33	17070.0
34	29645.0
35	38387.0
36	35464.0
37	52140.0
38	87386.0
39	130896.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.672865324559574	18.86740350097324	22.747345757590047	10.712385416877144
2	30.68863250108325	29.530846236914005	23.020347133822312	16.760174128180434
3	29.65477449322997	30.728594873590932	24.151865995837625	15.464764637341469
4	26.990990505568913	23.454769554060334	31.636728563442514	17.917511376928243
5	29.351240068340147	30.70883819504781	22.02083880570878	17.91908293090326
6	25.299998428446024	38.31875155752224	22.453016148839634	13.9282338651921
7	74.59717704004544	5.599446813000792	15.06020174262885	4.743174404324916
8	72.87430486800069	11.259061692473828	9.806496804132738	6.060136635392743
9	66.72301810205671	7.949818036500464	11.14052162121508	14.186642240227739
10	42.34686890321248	23.812410337233032	17.98351664387913	15.857204115675353
11	34.515366430260045	22.93660575772475	23.03561365815109	19.512414153864114
12	29.36605757724749	20.525168436909965	27.672595915306715	22.436178070535835
13	26.48944027982641	22.852864381627185	31.796802561183963	18.86089277736244
14	20.87629849647186	30.184612690522854	26.458907231168855	22.48018158183643
15	18.333299657176727	24.75579173766723	36.12149459273179	20.789414012424256
16	20.981143597376853	24.90351781131923	28.82297342502228	25.292365166281638
17	20.97710245858394	25.504524952909506	29.394345548797872	24.12402703970868
18	21.729427797197694	24.572593445721893	29.671612571533768	24.026366185546642
19	23.375069316755685	26.355858191949604	26.03862879670602	24.23044369458869
20	26.501788203915865	25.14082246154744	29.299603294875165	19.057786039661533
21	24.263895343485572	29.1018120017332	26.537035914498485	20.097256740282745
22	22.238611285104586	24.3186752249006	27.948964907199738	25.493748582795078
23	23.10925218726637	27.967823554899994	26.605510766267265	22.317413491566366
24	24.174765782330795	24.137048486930286	26.282444170545038	25.405741560193885
25	20.554129931592502	30.02139558483136	25.465460611244694	23.959013872331443
26	20.765167179666786	26.1445964361646	28.887407137998157	24.20282924617046
27	23.405602365413237	27.43955691158213	26.516156697401772	22.63868402560286
28	19.395445636580387	27.63083748111329	30.216268277734	22.757448604572325
29	23.839800277940544	23.60788381276955	29.61391408987942	22.938401819410487
30	21.589334985710085	26.863245618170755	29.719208206205845	21.82821118991332
31	25.185948511401623	26.778381703519607	24.002119352789173	24.033550432289598
32	28.215231052110486	27.072935819980735	24.481892330592096	20.229940797316683
33	23.391009364216615	24.831675343889685	25.486788843762838	26.290526448130862
34	26.02807693430231	24.50636367106028	27.941556152746067	21.524003241891343
35	24.570572876325436	23.196810194446126	27.802810387522758	24.429806541705677
36	22.7260175250719	27.435515772789216	28.9475752044704	20.890891497668488
37	24.89678257999771	25.835224810796127	29.421735489505384	19.846257119700773
38	23.12025306509152	27.555178382601554	23.787040965921975	25.537527586384957
39	27.94020910648176	23.263488984529175	26.157393375675486	22.638908533313575
40	25.78111845251325	22.064168793877226	27.910125073245638	24.244587680363882
41	23.332188344008674	24.68978647071634	28.272929533764835	23.705095651510153
42	25.019363790049372	23.348801914601758	29.399958241565805	22.23187605378307
43	24.17858241341299	23.421317905163452	28.788174729861097	23.61192495156246
44	23.356659684476863	23.66401074044888	28.61530379260876	24.364025782465497
45	22.39374611321026	22.85510945873436	27.36928599812761	27.381858429927775
46	26.436007444675685	24.606943125461644	26.451049461293746	22.505999968568922
47	21.49144962383733	23.778060657493285	32.42003596613526	22.31045375253413
48	23.30816601896192	23.697237881635044	27.74219330562908	25.252402793773953
49	22.089313657477565	21.585069339206456	32.13491117352426	24.190705829791725
50	22.804146208401527	22.002878188851398	29.589667257121942	25.60330834562513
51	21.4755095763764	21.856948176885137	27.62253069581675	29.045011550921718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	459.0
2	736.0
3	1281.5
4	1827.0
5	1317.0
6	807.0
7	771.5
8	736.0
9	779.0
10	822.0
11	795.5
12	769.0
13	751.5
14	734.0
15	789.5
16	845.0
17	859.0
18	873.0
19	867.5
20	862.0
21	1003.5
22	1145.0
23	1282.0
24	1419.0
25	1677.0
26	2295.5
27	2656.0
28	3238.5
29	3821.0
30	4538.5
31	5256.0
32	6741.0
33	8226.0
34	8523.0
35	8820.0
36	9913.0
37	11006.0
38	12015.5
39	13025.0
40	14986.0
41	16947.0
42	19838.5
43	22730.0
44	26359.0
45	29988.0
46	45301.0
47	60614.0
48	51203.5
49	41793.0
50	41383.0
51	40973.0
52	36337.5
53	31702.0
54	28626.5
55	25551.0
56	23395.5
57	21240.0
58	20771.0
59	20302.0
60	20328.5
61	20355.0
62	18355.0
63	16355.0
64	13411.0
65	10467.0
66	8811.0
67	7155.0
68	5952.0
69	4749.0
70	4003.0
71	3257.0
72	2522.0
73	1787.0
74	1555.5
75	1089.5
76	855.0
77	590.5
78	326.0
79	263.5
80	201.0
81	172.0
82	143.0
83	106.0
84	69.0
85	38.0
86	7.0
87	7.0
88	7.0
89	8.5
90	10.0
91	7.5
92	5.0
93	2.5
94	0.0
95	1.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.934177739559164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18226909570994	22.63833319590939
2	10.221901888155664	6.324122596890853
3	3.596674350601412	3.3378049089845283
4	1.7772834289309198	2.1991520593648897
5	1.1540416082153202	1.7849664113689707
6	0.8187367989332086	1.519616979607055
7	0.6814475700861163	1.4756014177264236
8	0.5383677039418168	1.3323169794379621
9	0.5492125832533141	1.5290495700444406
>10	7.340952180443394	46.4662253256663
>50	0.0932274206350849	1.9603853308358965
>100	0.03641696118558004	2.1507686895942384
>500	0.003641696118558004	0.9469600672914922
>1k	0.003641696118558004	1.693623322824208
>5k	0.0021850176711348023	4.641073144453354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC	7774	1.7453229431164812	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG	6764	1.5185701552919835	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	6061	1.3607412346577041	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2479	0.5565546148682476	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT	1410	0.31655587211142766	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1230	0.27614448418230925	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	1226	0.2752464533394399	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCGGTT	1172	0.26312303696070444	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTA	971	0.21799698710652216	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC	952	0.213731340602893	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.18881098471326996	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	732	0.1643396442450816	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT	707	0.1587269514771485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4901542143464915E-4	0.0	0.0	0.17601404520238248	0.0
2	4.4901542143464915E-4	0.0	0.0	1.2413031325560877	0.0
3	4.4901542143464915E-4	0.0	0.0	1.572901021285576	0.0
4	4.4901542143464915E-4	0.0	0.0	2.2291370597123157	0.0
5	4.4901542143464915E-4	0.0	0.0	4.690190584595628	0.0
6	4.4901542143464915E-4	0.0	0.0	5.455986385852422	0.0
7	4.4901542143464915E-4	0.0	0.0	6.430574358076328	0.0
8	4.4901542143464915E-4	0.0	0.0	7.651447288957139	0.0
9	4.4901542143464915E-4	0.0	0.0	8.075542354502165	0.0
10	4.4901542143464915E-4	0.0	0.0	10.569374005150207	0.0
11	4.4901542143464915E-4	0.0	0.0	11.78845087434528	0.0
12	4.4901542143464915E-4	0.0	0.0	14.5995119202369	0.0
13	4.4901542143464915E-4	0.0	0.0	15.104878777061598	0.0
14	4.4901542143464915E-4	0.0	0.0	15.353408812825677	0.0
15	4.4901542143464915E-4	0.0	0.0	15.905697781190295	0.0
16	4.4901542143464915E-4	0.0	0.0	16.595385468513918	0.0
17	4.4901542143464915E-4	0.0	0.0	17.374202716992315	0.0
18	4.4901542143464915E-4	0.0	0.0	18.112833085252312	0.0
19	4.4901542143464915E-4	0.0	0.0	18.885364117830626	0.0
20	4.4901542143464915E-4	0.0	0.0	19.424182623552205	0.0
21	4.4901542143464915E-4	0.0	0.0	20.00116744009573	0.0
22	4.4901542143464915E-4	0.0	0.0	20.651117262622385	0.0
23	4.4901542143464915E-4	0.0	0.0	21.225183478926585	0.0
24	4.4901542143464915E-4	0.0	0.0	21.7036094104652	0.0
25	4.4901542143464915E-4	0.0	0.0	22.117826136738667	0.0
26	4.4901542143464915E-4	0.0	0.0	22.51700084639407	0.0
27	4.4901542143464915E-4	0.0	0.0	22.99026310058619	0.0
28	4.4901542143464915E-4	0.0	0.0	23.404704334570372	0.0
29	4.4901542143464915E-4	0.0	0.0	23.785469411946952	0.0
30	4.4901542143464915E-4	0.0	0.0	24.25064938855325	0.0
31	4.4901542143464915E-4	0.0	0.0	24.707073564441572	0.0
32	4.4901542143464915E-4	0.0	0.0	25.133862722515204	0.0
33	4.4901542143464915E-4	0.0	0.0	25.546058879392213	0.0
34	4.4901542143464915E-4	0.0	0.0	25.954662912897742	0.0
35	4.4901542143464915E-4	0.0	0.0	26.40053522638235	0.0
36	4.4901542143464915E-4	0.0	0.0	26.84708106299911	0.0
37	4.4901542143464915E-4	0.0	0.0	27.292279853351562	0.0
38	4.4901542143464915E-4	0.0	0.0	27.710986733839373	0.0
39	4.4901542143464915E-4	0.0	0.0	28.14181703070592	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGA	20	7.028627E-4	45.000004	41
GGTCGAC	20	7.028627E-4	45.000004	12
GTTAGCG	30	2.1624965E-6	45.000004	1
GTCTACG	20	7.028627E-4	45.000004	1
CGATTGA	45	3.8380676E-10	45.000004	10
GTTAACG	40	6.7993824E-9	45.000004	1
GTCACCG	20	7.028627E-4	45.000004	18
CATCGAA	20	7.028627E-4	45.000004	30
TATACGG	20	7.028627E-4	45.000004	2
TCGACGC	20	7.028627E-4	45.000004	14
CCGTTTG	20	7.028627E-4	45.000004	5
CGGGTCA	35	1.2096825E-7	45.0	6
TACGGCT	855	0.0	43.157894	7
ACGGCTG	890	0.0	41.713486	8
TTAACGG	60	3.6379788E-12	41.250004	2
TAATACG	110	0.0	40.909092	4
ACGGGGT	105	0.0	40.714287	5
CGCATGG	50	1.0786607E-9	40.500004	2
GCATAAG	40	3.4518598E-7	39.375004	1
ACAACGA	80	0.0	39.375004	13
>>END_MODULE