FastQCFastQC Report
Sat 18 Jun 2016
SRR3550929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507140
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC134762.657254407067082No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG121432.394407855818906No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC114072.249280277635367No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC36350.7167646014907126No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT24060.474425208029341No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT22100.43577710296959415No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT17660.34822731395669837No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC16590.32712860354142836No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA16560.32653705091296287No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.25062113025988875No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC9640.190085577946918No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT6660.1313246835193438TruSeq Adapter, Index 27 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT6640.13093031510036676No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG6320.12462042039673464No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC6110.12047955199747604No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG5120.10095831525811413No Hit
GAATATGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC5110.10076113104862564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACG650.045.00000424
CTCGAAT850.045.00000443
CGGCTAT351.2101373E-745.00000431
CGAACGG207.0295535E-445.02
CGAACCC253.8877115E-545.034
TCGGGCA207.0295535E-445.05
ACGGGTA551.8189894E-1245.05
TATTGCG207.0295535E-445.01
CTAACGG253.8877115E-545.02
CGAATAT551.8189894E-1245.014
ACGTCCG207.0295535E-445.023
ATACGGT453.8380676E-1045.028
CGATCCA207.0295535E-445.034
TCGGTCA207.0295535E-445.019
AATCGCG207.0295535E-445.018
TAATCCG207.0295535E-445.04
CCGATGA8850.042.96610318
TAATACG2000.042.754
CGATGAA9000.042.7519
CGAATGC900.042.545