##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507140
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828406357218913	33.0	31.0	34.0	30.0	34.0
2	31.87664944591237	33.0	31.0	34.0	30.0	34.0
3	31.933048073510275	33.0	31.0	34.0	30.0	34.0
4	35.65334030050874	37.0	35.0	37.0	33.0	37.0
5	33.6431774263517	37.0	35.0	37.0	32.0	37.0
6	34.53097961115274	37.0	35.0	37.0	30.0	37.0
7	35.53549512955003	37.0	35.0	37.0	32.0	37.0
8	35.42715029380447	37.0	35.0	37.0	33.0	37.0
9	37.259878928895375	39.0	37.0	39.0	34.0	39.0
10	37.02545845328706	39.0	37.0	39.0	33.0	39.0
11	36.987892889537406	39.0	37.0	39.0	33.0	39.0
12	36.90229719604054	39.0	35.0	39.0	33.0	39.0
13	36.75962653310723	39.0	35.0	39.0	32.0	39.0
14	37.878873683795405	40.0	37.0	41.0	33.0	41.0
15	37.83001143668415	40.0	36.0	41.0	33.0	41.0
16	38.01707418069961	40.0	37.0	41.0	33.0	41.0
17	37.78684781322712	40.0	36.0	41.0	33.0	41.0
18	37.81542769255038	40.0	36.0	41.0	33.0	41.0
19	37.74936309500335	40.0	36.0	41.0	33.0	41.0
20	37.52259533856529	40.0	36.0	41.0	32.0	41.0
21	37.68724218164609	40.0	36.0	41.0	33.0	41.0
22	37.705880033126945	40.0	36.0	41.0	33.0	41.0
23	37.63159285404425	40.0	36.0	41.0	33.0	41.0
24	37.617468549118584	40.0	36.0	41.0	33.0	41.0
25	37.587748945064476	39.0	36.0	41.0	33.0	41.0
26	37.4883957092716	39.0	36.0	41.0	33.0	41.0
27	37.41124935915132	40.0	36.0	41.0	33.0	41.0
28	37.27354182277083	39.0	35.0	41.0	32.0	41.0
29	37.2299562251055	39.0	35.0	41.0	32.0	41.0
30	37.08059510194423	39.0	35.0	41.0	31.0	41.0
31	37.07166068541231	39.0	35.0	41.0	31.0	41.0
32	36.926197894072644	39.0	35.0	41.0	31.0	41.0
33	36.65613440075719	39.0	35.0	41.0	30.0	41.0
34	36.55851047048152	39.0	35.0	41.0	30.0	41.0
35	36.52055448199708	39.0	35.0	41.0	30.0	41.0
36	36.43397483929487	39.0	35.0	41.0	30.0	41.0
37	36.39764167685452	39.0	35.0	41.0	30.0	41.0
38	36.01625586623023	39.0	35.0	41.0	29.0	41.0
39	36.09583349765351	39.0	35.0	41.0	30.0	41.0
40	35.830340734314	39.0	35.0	41.0	28.0	41.0
41	35.870079268052216	39.0	35.0	40.0	28.0	41.0
42	35.959626533107226	39.0	35.0	41.0	28.0	41.0
43	35.954235516819814	39.0	35.0	41.0	29.0	41.0
44	35.93655992428126	39.0	35.0	40.0	29.0	41.0
45	35.87037307252435	38.0	35.0	40.0	28.0	41.0
46	35.70563158102299	38.0	35.0	40.0	28.0	41.0
47	35.47213787119927	38.0	35.0	40.0	28.0	41.0
48	35.49137121899278	38.0	35.0	40.0	28.0	41.0
49	35.45247071814489	38.0	35.0	40.0	28.0	41.0
50	35.25739835154001	38.0	34.0	40.0	27.0	41.0
51	34.02586465275861	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	6.0
10	9.0
11	6.0
12	6.0
13	7.0
14	9.0
15	21.0
16	35.0
17	60.0
18	233.0
19	488.0
20	1188.0
21	2142.0
22	2989.0
23	3364.0
24	3758.0
25	4265.0
26	4808.0
27	5357.0
28	5859.0
29	6847.0
30	8487.0
31	11521.0
32	15225.0
33	20733.0
34	33342.0
35	41338.0
36	40825.0
37	59854.0
38	97021.0
39	137247.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.87470915329101	18.446385613440075	20.782426943250385	9.896478290018536
2	28.228497061955277	34.72098434357377	21.1286824151122	15.921836179358756
3	28.141144457151874	34.17399534645266	23.039200220846315	14.645659975549158
4	25.695468706865952	21.870292226998462	35.48842528690302	16.94581377923256
5	31.93358835824427	29.770280395945893	20.08616949954648	18.20996174626336
6	23.36139921915053	40.95180817920101	22.302520014197263	13.384272587451196
7	71.06578065228535	5.642426154513547	18.91390937413732	4.377883819063769
8	69.60444847576606	14.874393658555823	10.053239736561896	5.467918129116221
9	63.62464013881768	7.392238829514533	10.573806049611548	18.409314982056237
10	43.449540560791895	21.887841621642938	18.566076428599597	16.096541388965573
11	35.38510076113105	22.272153645936033	23.052214378672556	19.290531214260362
12	27.939030642426154	19.61509642307844	27.00477185786962	25.441101076625785
13	26.311274993098554	21.628938754584535	33.22139843041369	18.838387821903222
14	21.51161414993887	30.6877785226959	25.330283550893245	22.47032377647198
15	17.92779114248531	23.409314982056237	36.90598256891588	21.756911306542573
16	20.30602989312616	24.316559529912844	27.422605197775763	27.95480537918523
17	20.601214654730448	25.88575146902236	30.19422644634618	23.318807429901014
18	20.91335725835075	24.80498481681587	28.479512560634145	25.802145364199237
19	21.806404543124184	26.11882320463777	25.860314705998345	26.214457546239693
20	27.3173088299089	24.73636471191387	30.03154947351816	17.91477698465907
21	23.432385534566393	30.761525417044606	26.539811491895733	19.266277556493275
22	20.990653468470242	23.047087589225853	29.897464211065977	26.06479473123792
23	24.069487715423747	28.42903340300509	27.314153882557086	20.187324999014077
24	24.502504239460503	24.19174192530662	25.330480735102732	25.975273100130142
25	20.224987183026382	33.659936112316124	23.857909058642583	22.257167646014906
26	21.427810860906256	24.217178688330637	29.199826477895652	25.15518397286745
27	26.66482628071144	26.2538943881374	24.206333556808772	22.87494577434239
28	19.490476002681707	28.5591749812675	29.626730291438264	22.323618724612533
29	28.06976377331703	22.476042118547145	26.83499625350002	22.6191978546358
30	24.142248688725008	27.96900264226841	27.99227037898805	19.896478290018536
31	25.451749023938163	26.394486729502702	22.875142958551876	25.27862128800726
32	31.17087983594274	27.752691564459518	22.8301849587885	18.246243640809244
33	24.209685688370076	23.99929013684584	25.71853925937611	26.072484915407973
34	26.89415151634657	24.592223054777772	27.71798714358954	20.795638285286113
35	25.75501833813148	25.13408526245218	24.686082738494303	24.424813660922034
36	24.847773790274875	29.163938951768742	23.554639744449265	22.43364751350712
37	27.439365855582288	25.527664944591237	27.385731750601412	19.647237449225067
38	21.36747249280278	29.054304531293134	25.078084946957446	24.50013802894664
39	27.66021217020941	23.28666640375439	27.565563749654924	21.487557676381275
40	22.12387112040068	25.20349410419214	31.232006940884173	21.44062783452301
41	20.321213077256772	25.848089285010055	27.11026540994597	26.7204322277872
42	22.107307646803644	27.082265252198606	28.255314114445717	22.555112986552036
43	24.956816658122015	23.212130772567733	24.406475529439604	27.424577039870645
44	24.199234925267184	22.50838032890326	28.853571006033835	24.438813739795716
45	21.582600465354734	22.433055960878654	27.610521749418304	28.373821824348305
46	28.025988878810587	26.192767283195963	24.658082580746935	21.12316125724652
47	20.307015814173603	23.808218637851482	34.542138265567694	21.342627282407225
48	23.070158141736012	25.14118389399377	26.399219150530424	25.389438813739794
49	21.637417675592538	22.601845644200814	32.4819576448318	23.278779035374846
50	22.563000354931578	22.732381590882202	28.795204480025237	25.909413574160983
51	22.417084039910083	22.036124147178292	26.190795441101077	29.355996371810544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	422.5
2	735.0
3	1679.0
4	2623.0
5	1858.0
6	1093.0
7	1039.0
8	985.0
9	976.5
10	968.0
11	949.0
12	930.0
13	949.5
14	969.0
15	969.5
16	970.0
17	973.0
18	976.0
19	1068.0
20	1160.0
21	1134.0
22	1108.0
23	1327.0
24	1546.0
25	1869.0
26	2701.0
27	3210.0
28	3708.5
29	4207.0
30	5342.5
31	6478.0
32	7650.0
33	8822.0
34	9247.0
35	9672.0
36	10746.0
37	11820.0
38	13459.5
39	15099.0
40	16934.0
41	18769.0
42	20700.0
43	22631.0
44	26300.5
45	29970.0
46	55962.5
47	81955.0
48	64186.0
49	46417.0
50	45769.5
51	45122.0
52	39115.5
53	33109.0
54	30688.0
55	28267.0
56	26930.5
57	25594.0
58	24418.0
59	23242.0
60	23041.5
61	22841.0
62	19967.5
63	17094.0
64	14408.0
65	11722.0
66	10054.5
67	8387.0
68	7107.0
69	5827.0
70	5054.0
71	4281.0
72	3344.5
73	2408.0
74	2032.5
75	1424.0
76	1191.0
77	866.0
78	541.0
79	396.0
80	251.0
81	178.0
82	105.0
83	68.5
84	32.0
85	32.0
86	32.0
87	20.5
88	9.0
89	6.0
90	3.0
91	4.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.19581511388843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6842098175168	21.512705666451833
2	9.634535082103453	5.625762099307286
3	3.4358549783278174	3.009377601161763
4	1.7682752086189852	2.0650494424524957
5	1.0881386568726903	1.5884547522164973
6	0.8368088234360643	1.4658789416825904
7	0.7074584202717398	1.4458377667312106
8	0.6008381863887094	1.403356848253504
9	0.5354779387931569	1.4070343408713963
>10	7.55120151525763	45.386774743387775
>50	0.11044751596253792	2.0368426818423813
>100	0.035234790368417014	1.9371370960179288
>500	0.004743144857286905	0.9020981574593298
>1k	0.004743144857286905	2.8888902178461824
>5k	0.0	0.0
>10k+	0.002032776367408674	7.324799644317793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	13476	2.657254407067082	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	12143	2.394407855818906	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	11407	2.249280277635367	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3635	0.7167646014907126	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	2406	0.474425208029341	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	2210	0.43577710296959415	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	1766	0.34822731395669837	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	1659	0.32712860354142836	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	1656	0.32653705091296287	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	0.25062113025988875	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	964	0.190085577946918	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	666	0.1313246835193438	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT	664	0.13093031510036676	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	632	0.12462042039673464	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	611	0.12047955199747604	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG	512	0.10095831525811413	No Hit
GAATATGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	511	0.10076113104862564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14098670978428046	0.0
2	0.0	0.0	0.0	1.2467957565958119	0.0
3	0.0	0.0	0.0	1.519107149899436	0.0
4	0.0	0.0	0.0	2.188350356903419	0.0
5	1.9718420948850417E-4	0.0	0.0	5.38095989273179	0.0
6	1.9718420948850417E-4	0.0	0.0	6.044287573451118	0.0
7	1.9718420948850417E-4	0.0	0.0	7.099420278424104	0.0
8	1.9718420948850417E-4	0.0	0.0	8.231454825097606	0.0
9	1.9718420948850417E-4	0.0	0.0	8.545372086603304	0.0
10	1.9718420948850417E-4	0.0	0.0	11.82040462199787	0.0
11	1.9718420948850417E-4	0.0	0.0	12.806917222068856	0.0
12	1.9718420948850417E-4	0.0	0.0	16.494064755294396	0.0
13	1.9718420948850417E-4	0.0	0.0	16.947588437117954	0.0
14	1.9718420948850417E-4	0.0	0.0	17.178688330638483	0.0
15	1.9718420948850417E-4	0.0	0.0	17.805536932602436	0.0
16	3.9436841897700834E-4	0.0	0.0	18.418582639902198	0.0
17	3.9436841897700834E-4	0.0	0.0	19.075008873289427	0.0
18	3.9436841897700834E-4	0.0	0.0	19.65966005442284	0.0
19	3.9436841897700834E-4	0.0	0.0	20.5523129707773	0.0
20	3.9436841897700834E-4	0.0	0.0	21.040935441889815	0.0
21	3.9436841897700834E-4	0.0	0.0	21.519304334108924	0.0
22	3.9436841897700834E-4	0.0	0.0	22.132350041408685	0.0
23	3.9436841897700834E-4	0.0	0.0	22.621366880940176	0.0
24	3.9436841897700834E-4	0.0	0.0	23.031115668257286	0.0
25	3.9436841897700834E-4	0.0	0.0	23.386441613755572	0.0
26	3.9436841897700834E-4	0.0	0.0	23.72954213826557	0.0
27	3.9436841897700834E-4	0.0	0.0	24.173009425405212	0.0
28	3.9436841897700834E-4	0.0	0.0	24.496983081594827	0.0
29	3.9436841897700834E-4	0.0	0.0	24.852111842883623	0.0
30	3.9436841897700834E-4	0.0	0.0	25.249043656583982	0.0
31	3.9436841897700834E-4	0.0	0.0	25.624088023031117	0.0
32	3.9436841897700834E-4	0.0	0.0	25.987301336908942	0.0
33	3.9436841897700834E-4	0.0	0.0	26.32231730882991	0.0
34	3.9436841897700834E-4	0.0	0.0	26.63426272824072	0.0
35	3.9436841897700834E-4	0.0	0.0	26.992743621090824	0.0
36	5.915526284655125E-4	0.0	0.0	27.35299917182632	0.0
37	5.915526284655125E-4	0.0	0.0	27.72745198564499	0.0
38	5.915526284655125E-4	0.0	0.0	28.09401743108412	0.0
39	7.887368379540167E-4	0.0	0.0	28.428244666167135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACACG	65	0.0	45.000004	24
CTCGAAT	85	0.0	45.000004	43
CGGCTAT	35	1.2101373E-7	45.000004	31
CGAACGG	20	7.0295535E-4	45.0	2
CGAACCC	25	3.8877115E-5	45.0	34
TCGGGCA	20	7.0295535E-4	45.0	5
ACGGGTA	55	1.8189894E-12	45.0	5
TATTGCG	20	7.0295535E-4	45.0	1
CTAACGG	25	3.8877115E-5	45.0	2
CGAATAT	55	1.8189894E-12	45.0	14
ACGTCCG	20	7.0295535E-4	45.0	23
ATACGGT	45	3.8380676E-10	45.0	28
CGATCCA	20	7.0295535E-4	45.0	34
TCGGTCA	20	7.0295535E-4	45.0	19
AATCGCG	20	7.0295535E-4	45.0	18
TAATCCG	20	7.0295535E-4	45.0	4
CCGATGA	885	0.0	42.966103	18
TAATACG	200	0.0	42.75	4
CGATGAA	900	0.0	42.75	19
CGAATGC	90	0.0	42.5	45
>>END_MODULE