FastQCFastQC Report
Sat 18 Jun 2016
SRR3550928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561926
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC117242.0863957175855896No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG98481.752543929271826No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC91911.635624619611835No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC37090.6600513234838752TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT20690.3681979477724825No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT19780.35200364460800887No Hit
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT16050.28562479757121045TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA15930.2834892850660051No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC15520.2761929506732203No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC13950.2482533287301175TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.20340756612080593No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT9250.1646124222762428TruSeq Adapter, Index 16 (96% over 25bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC6360.11318216277588153No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG5720.10179276274811985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1850.045.04
TTTCGCG207.030206E-445.01
ATCGTGA253.888252E-545.030
GCCGATT207.030206E-445.09
CGTAGGT207.030206E-445.011
TTAAGCG207.030206E-445.01
TGCGAAG406.8048394E-945.01
TCCACGT207.030206E-445.014
CGAATAT502.1827873E-1145.014
CGGTCTA351.2104465E-745.031
GCGTAAG502.1827873E-1145.01
ACGGTTA351.2104465E-745.034
AATACGG1900.043.8157925
AACACGT1400.043.39285741
CACAACG950.042.6315812
CGGGCTC800.042.18756
TACGGCT15600.042.1153837
ACGGCTG15650.041.980838
CGATGAA6650.041.27819419
CCGATGA6600.041.2518
Produced by FastQC (version 0.11.5)