FastQCFastQC Report
Sat 18 Jun 2016
SRR3550927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814517
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC162181.9911186629622217No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC161511.9828929291837987No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG137791.6916773989984246No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC46830.5749419594680037TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT31080.3815758296020832No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT27790.3411837935856465No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT27120.3329580598072232No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC22160.2720630754177015No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.2665383288501038No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA19740.2423522160986204No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC15520.19054237050914838TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT11400.13996024637914248TruSeq Adapter, Index 22 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9890.12142165234120343No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT9280.11393255143845986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG253.8898004E-545.0000041
CACGCGT302.1647193E-645.00000441
GTATACG207.032074E-445.0000041
CTTACGT302.1647193E-645.00000422
TGTCCGC207.032074E-445.00000428
CGACGAA351.2113378E-745.019
ACGGGCT1350.043.3333365
TAATACG1800.042.54
TACGGCT19600.041.900517
ACGGCTG19950.041.1654138
CCGATGA8200.041.15853518
ATTACGG556.002665E-1140.9090922
CGATGAA8400.040.71428719
ATAATAC2600.040.6730773
TTACGGG2600.040.6730773
TACGGGA3550.040.5633774
GCGAACC451.9270374E-840.033
GATGAAT8900.039.69101320
ACCACCG14000.039.53571314
CATACGA1200.039.37500418
Produced by FastQC (version 0.11.5)