##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814517
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876142548283216	33.0	31.0	34.0	30.0	34.0
2	31.931572944456654	33.0	31.0	34.0	30.0	34.0
3	31.983101641831908	33.0	31.0	34.0	30.0	34.0
4	35.68953011416582	37.0	35.0	37.0	33.0	37.0
5	33.70402459371628	37.0	35.0	37.0	32.0	37.0
6	34.59848229073181	37.0	35.0	37.0	30.0	37.0
7	35.60638758920931	37.0	35.0	37.0	32.0	37.0
8	35.52142435332841	37.0	35.0	37.0	33.0	37.0
9	37.39112873027819	39.0	37.0	39.0	34.0	39.0
10	37.08072391368136	39.0	37.0	39.0	33.0	39.0
11	37.04701068240442	39.0	37.0	39.0	33.0	39.0
12	36.994820243162515	39.0	37.0	39.0	33.0	39.0
13	36.91530686284019	39.0	37.0	39.0	33.0	39.0
14	38.06202571585369	40.0	37.0	41.0	33.0	41.0
15	38.034408121622995	40.0	37.0	41.0	33.0	41.0
16	38.12718826003631	40.0	37.0	41.0	33.0	41.0
17	37.98874056649524	40.0	37.0	41.0	33.0	41.0
18	37.98949315974989	40.0	37.0	41.0	33.0	41.0
19	37.91190852984038	40.0	37.0	41.0	33.0	41.0
20	37.808697669907446	40.0	36.0	41.0	33.0	41.0
21	37.85937678403275	40.0	36.0	41.0	33.0	41.0
22	37.91478139805553	40.0	36.0	41.0	33.0	41.0
23	37.894523993974346	40.0	36.0	41.0	33.0	41.0
24	37.825023909875426	40.0	36.0	41.0	33.0	41.0
25	37.706832392694075	40.0	36.0	41.0	33.0	41.0
26	37.67633579164094	40.0	36.0	41.0	33.0	41.0
27	37.6147901148779	40.0	36.0	41.0	33.0	41.0
28	37.49920627807646	40.0	36.0	41.0	33.0	41.0
29	37.49637392466947	40.0	36.0	41.0	33.0	41.0
30	37.31027590584359	39.0	35.0	41.0	32.0	41.0
31	37.36155537576257	40.0	35.0	41.0	33.0	41.0
32	37.189443559802925	40.0	35.0	41.0	32.0	41.0
33	37.06944360891178	39.0	35.0	41.0	31.0	41.0
34	37.028798662274696	40.0	35.0	41.0	31.0	41.0
35	36.88857077261739	39.0	35.0	41.0	31.0	41.0
36	36.847491212583655	39.0	35.0	41.0	31.0	41.0
37	36.69136433002626	39.0	35.0	41.0	31.0	41.0
38	36.61273736459767	39.0	35.0	41.0	31.0	41.0
39	36.52365757866318	39.0	35.0	41.0	30.0	41.0
40	36.39645949685519	39.0	35.0	41.0	30.0	41.0
41	36.33103790344462	39.0	35.0	41.0	30.0	41.0
42	36.416810207767305	39.0	35.0	41.0	30.0	41.0
43	36.38484893501302	39.0	35.0	41.0	30.0	41.0
44	36.246304251476644	39.0	35.0	41.0	30.0	41.0
45	36.25326788759473	39.0	35.0	41.0	30.0	41.0
46	36.04911745242886	38.0	35.0	40.0	29.0	41.0
47	35.90663055528614	38.0	35.0	40.0	29.0	41.0
48	35.882418660384005	38.0	35.0	40.0	29.0	41.0
49	35.819938687590316	38.0	35.0	40.0	29.0	41.0
50	35.69601616663618	38.0	35.0	40.0	28.0	41.0
51	34.45984184492159	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	10.0
10	18.0
11	14.0
12	12.0
13	17.0
14	23.0
15	32.0
16	70.0
17	109.0
18	248.0
19	518.0
20	1145.0
21	2090.0
22	3041.0
23	3789.0
24	4561.0
25	5646.0
26	6810.0
27	7818.0
28	9075.0
29	10698.0
30	13254.0
31	17369.0
32	23153.0
33	32161.0
34	52711.0
35	65692.0
36	64975.0
37	95839.0
38	156622.0
39	236844.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.59014606202203	19.604379036901623	21.146642734283017	10.658832166793326
2	29.11087184184001	32.21418337493263	22.13630900275869	16.53863578046867
3	29.558130769523533	31.391732769236246	23.740695405989072	15.309441055251149
4	27.290406461743586	23.308046363673196	32.43234947827977	16.969197696303453
5	28.891478017033407	31.500754434836843	21.023256727606668	18.584510820523086
6	24.605011313453247	38.7732852721306	22.84421319628688	13.777490218129271
7	74.35093435741673	5.728916646306952	15.554739802852488	4.365409193423832
8	72.67632228670489	11.930260510216485	9.860690445994374	5.5327267570842595
9	66.67767523575321	7.630657187020037	10.880558662372916	14.811108914853834
10	41.34278351464733	24.036576277720417	18.638898881177436	15.981741326454818
11	33.27984560174926	23.302153300667758	23.66420835906433	19.753792738518655
12	27.751293097627183	20.49570481647406	27.98419185848791	23.768810227410846
13	25.05914548130978	23.302644389251544	32.17636955398107	19.461840575457604
14	21.530551234658084	29.339719121884507	26.563718129885565	22.566011513571844
15	18.97394406746575	24.272912658667654	35.77653996171964	20.976603312146956
16	21.691996606577888	25.139192920466975	27.26265995675965	25.906150516195485
17	20.917058821362843	26.21504523539717	29.137022308926642	23.73087363431334
18	21.843742978967903	24.84159323869238	29.032174896288232	24.282488886051485
19	22.36024539696532	26.350462912376294	26.147397782980587	25.141893907677805
20	25.767295219129867	25.020595027482546	30.58720689684807	18.624902856539517
21	23.752849848437783	29.877092804692847	27.07371362414781	19.296343722721566
22	21.902182520438494	23.181099964764392	28.550416995593707	26.366300519203406
23	22.719231151713224	28.0608016775586	27.702184239248535	21.517782931479637
24	24.48923717982559	24.092928692709915	26.331556001900513	25.086278125563982
25	21.04314581525002	31.684298793027033	24.406365981311623	22.866189410411323
26	21.95018642950362	24.86860311080063	28.479700239528455	24.70151022016729
27	24.55013216421511	26.508961752793375	25.619354783264193	23.32155129972732
28	19.878897555238257	26.485635045063518	29.496744696550227	24.138722703147998
29	25.38400057948453	24.06616436489355	26.874086114838608	23.67574894078331
30	20.938175630465665	27.74478617389201	29.833140376443957	21.483897819198372
31	23.837317084849058	25.049937570363785	24.015459468617596	27.097285876169558
32	24.329510617949044	30.084332187050734	24.04664359368804	21.539513601312187
33	21.455414681338755	27.322081675397815	23.9427783582172	27.27972528504623
34	23.40000270098721	25.201070082024067	27.76221981861643	23.636707398372288
35	20.93031821312508	24.863201136378983	26.685508098664606	27.52097255183133
36	23.2480107843053	26.287235257213787	24.05781585896918	26.406938099511734
37	20.41553460517092	26.942838516568713	30.30397155614923	22.337655322111143
38	19.9264103757196	27.85724545957911	23.86837843777355	28.347965726927736
39	23.261884036797266	26.370720316457486	27.673823873534868	22.69357177321038
40	21.07899528186643	25.358709517419527	29.16452326961868	24.39777193109536
41	19.605483986215145	25.406590654338707	27.381748938327867	27.606176421118285
42	20.766540170432293	23.24445039207285	28.97373535481764	27.01527408267722
43	23.374834411068154	23.089266399596326	25.306163038954377	28.229736150381147
44	21.896412229579003	23.19914747021855	28.49725665639882	26.407183643803627
45	21.14295956990462	22.739488555794416	26.865123748184505	29.25242812611646
46	25.19173939893213	25.966922728439062	25.689825995037552	23.15151187759126
47	19.27264869855387	23.919451650487346	33.61354029443216	23.19435935652663
48	21.505628489030922	24.955894106568678	26.912391024373957	26.626086380026443
49	20.732532286005082	22.487314568020068	32.05273800301283	24.727415142962027
50	21.13706650689918	23.161210877121043	29.158630206613246	26.54309240936653
51	20.96432609755229	22.647409446334454	26.362249038387166	30.026015417726086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	771.0
2	1322.0
3	2795.5
4	4269.0
5	2987.0
6	1705.0
7	1543.0
8	1381.0
9	1432.0
10	1483.0
11	1553.0
12	1623.0
13	1601.5
14	1580.0
15	1546.5
16	1513.0
17	1616.0
18	1719.0
19	1630.0
20	1541.0
21	1804.0
22	2067.0
23	2181.0
24	2295.0
25	3067.0
26	4524.0
27	5209.0
28	6842.0
29	8475.0
30	9359.0
31	10243.0
32	11810.5
33	13378.0
34	15401.0
35	17424.0
36	19102.0
37	20780.0
38	22298.0
39	23816.0
40	27584.0
41	31352.0
42	34666.0
43	37980.0
44	43906.5
45	49833.0
46	85028.5
47	120224.0
48	98266.0
49	76308.0
50	76222.0
51	76136.0
52	65986.5
53	55837.0
54	51350.0
55	46863.0
56	42926.0
57	38989.0
58	37212.0
59	35435.0
60	34008.5
61	32582.0
62	29708.0
63	26834.0
64	23361.0
65	19888.0
66	16697.5
67	13507.0
68	11485.5
69	9464.0
70	8366.5
71	7269.0
72	5710.5
73	4152.0
74	3300.0
75	2097.5
76	1747.0
77	1317.5
78	888.0
79	650.5
80	413.0
81	310.5
82	208.0
83	192.5
84	177.0
85	115.0
86	53.0
87	38.0
88	23.0
89	19.5
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.208287405017806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42811231403266	20.430730083571426
2	10.679164563987701	6.0248188653289345
3	3.8586198819007476	3.2653517584611635
4	1.8334048998688075	2.068688493810688
5	1.1420717030644945	1.6107943418590707
6	0.7785759792256595	1.3177376993184342
7	0.6571506140090679	1.2975965371846194
8	0.516565832301302	1.1657149968933886
9	0.45584861222334355	1.1572837800097129
>10	7.418433053760494	46.56919677761009
>50	0.16868603131879897	2.97063665717519
>100	0.0520042427760509	2.5299351014963483
>500	0.006118146208947165	1.1493989614750255
>1k	0.003933093991466035	2.7533060708434896
>5k	0.0	0.0
>10k+	0.0013110313304886781	5.6888098749624065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	16218	1.9911186629622217	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	16151	1.9828929291837987	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	13779	1.6916773989984246	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	4683	0.5749419594680037	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	3108	0.3815758296020832	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	2779	0.3411837935856465	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT	2712	0.3329580598072232	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	2216	0.2720630754177015	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2171	0.2665383288501038	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA	1974	0.2423522160986204	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1552	0.19054237050914838	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1140	0.13996024637914248	TruSeq Adapter, Index 22 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	989	0.12142165234120343	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	928	0.11393255143845986	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.910885837864649E-4	0.0	0.0	0.15186914453596426	0.0
2	4.910885837864649E-4	0.0	0.0	1.1083869336060512	0.0
3	4.910885837864649E-4	0.0	0.0	1.3905173249913751	0.0
4	4.910885837864649E-4	0.0	0.0	2.112294771011532	0.0
5	4.910885837864649E-4	0.0	0.0	5.0394282746707555	0.0
6	4.910885837864649E-4	0.0	0.0	5.736528519355643	0.0
7	4.910885837864649E-4	0.0	0.0	6.688872055463545	0.0
8	4.910885837864649E-4	0.0	0.0	7.907385603983711	0.0
9	4.910885837864649E-4	0.0	0.0	8.268949573796496	0.0
10	4.910885837864649E-4	0.0	0.0	10.868895308507987	0.0
11	4.910885837864649E-4	0.0	0.0	11.93480307961651	0.0
12	4.910885837864649E-4	0.0	0.0	15.002879006822448	0.0
13	4.910885837864649E-4	0.0	0.0	15.492003236273767	0.0
14	4.910885837864649E-4	0.0	0.0	15.723183187091246	0.0
15	4.910885837864649E-4	0.0	0.0	16.289653868488934	0.0
16	4.910885837864649E-4	0.0	0.0	16.93150664749784	0.0
17	4.910885837864649E-4	0.0	0.0	17.592634653420372	0.0
18	4.910885837864649E-4	0.0	0.0	18.278930949261955	0.0
19	4.910885837864649E-4	0.0	0.0	19.11390431384489	0.0
20	4.910885837864649E-4	0.0	0.0	19.60155527754485	0.0
21	4.910885837864649E-4	0.0	0.0	20.130457682282874	0.0
22	4.910885837864649E-4	0.0	0.0	20.75941938596739	0.0
23	4.910885837864649E-4	0.0	0.0	21.30366830894874	0.0
24	4.910885837864649E-4	0.0	0.0	21.781497500972968	0.0
25	4.910885837864649E-4	0.0	0.0	22.196958442856317	0.0
26	4.910885837864649E-4	0.0	0.0	22.587251094820612	0.0
27	4.910885837864649E-4	0.0	0.0	23.041139718385253	0.0
28	4.910885837864649E-4	0.0	0.0	23.434992762581995	0.0
29	4.910885837864649E-4	0.0	0.0	23.83044184467605	0.0
30	4.910885837864649E-4	0.0	0.0	24.324108643527392	0.0
31	8.594050216263135E-4	0.0	0.0	24.734044838843143	0.0
32	8.594050216263135E-4	0.0	0.0	25.153066172958944	0.0
33	0.0011049493135195459	0.0	0.0	25.54139447058809	0.0
34	0.0011049493135195459	0.0	0.0	25.920392085125297	0.0
35	0.0011049493135195459	0.0	0.0	26.337694609197843	0.0
36	0.0011049493135195459	0.0	0.0	26.76125851271367	0.0
37	0.0011049493135195459	0.0	0.0	27.160759075623957	0.0
38	0.0011049493135195459	0.0	0.0	27.577202194674882	0.0
39	0.0012277214594661621	0.0	0.0	27.986278984969005	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	25	3.8898004E-5	45.000004	1
CACGCGT	30	2.1647193E-6	45.000004	41
GTATACG	20	7.032074E-4	45.000004	1
CTTACGT	30	2.1647193E-6	45.000004	22
TGTCCGC	20	7.032074E-4	45.000004	28
CGACGAA	35	1.2113378E-7	45.0	19
ACGGGCT	135	0.0	43.333336	5
TAATACG	180	0.0	42.5	4
TACGGCT	1960	0.0	41.90051	7
ACGGCTG	1995	0.0	41.165413	8
CCGATGA	820	0.0	41.158535	18
ATTACGG	55	6.002665E-11	40.909092	2
CGATGAA	840	0.0	40.714287	19
ATAATAC	260	0.0	40.673077	3
TTACGGG	260	0.0	40.673077	3
TACGGGA	355	0.0	40.563377	4
GCGAACC	45	1.9270374E-8	40.0	33
GATGAAT	890	0.0	39.691013	20
ACCACCG	1400	0.0	39.535713	14
CATACGA	120	0.0	39.375004	18
>>END_MODULE