##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1167631
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00250421580105	33.0	31.0	34.0	30.0	34.0
2	32.072491223682825	33.0	31.0	34.0	30.0	34.0
3	32.015492908290376	33.0	31.0	34.0	30.0	34.0
4	35.75387001544152	37.0	35.0	37.0	33.0	37.0
5	33.742356960375325	37.0	35.0	37.0	32.0	37.0
6	34.67560642017898	37.0	35.0	37.0	31.0	37.0
7	35.73295416103204	37.0	35.0	37.0	33.0	37.0
8	35.7367558757861	37.0	35.0	37.0	35.0	37.0
9	37.621893389264244	39.0	37.0	39.0	35.0	39.0
10	37.277552582964994	39.0	37.0	39.0	34.0	39.0
11	37.23075869003135	39.0	37.0	39.0	34.0	39.0
12	37.25796848490662	39.0	37.0	39.0	34.0	39.0
13	37.26550168674864	39.0	37.0	39.0	34.0	39.0
14	38.45951931731857	40.0	38.0	41.0	34.0	41.0
15	38.529017300842476	40.0	38.0	41.0	34.0	41.0
16	38.489908198737446	40.0	38.0	41.0	34.0	41.0
17	38.418148370504035	40.0	38.0	41.0	34.0	41.0
18	38.291207581847345	40.0	38.0	41.0	34.0	41.0
19	38.1441037451044	40.0	37.0	41.0	34.0	41.0
20	38.075623206304044	40.0	36.0	41.0	34.0	41.0
21	37.98857001912419	40.0	36.0	41.0	34.0	41.0
22	38.03536648136269	40.0	36.0	41.0	34.0	41.0
23	38.02843021468255	40.0	36.0	41.0	34.0	41.0
24	37.96161287255991	40.0	36.0	41.0	34.0	41.0
25	37.776622922824075	40.0	35.0	41.0	33.0	41.0
26	37.803115025209166	40.0	36.0	41.0	33.0	41.0
27	37.801982818201985	40.0	36.0	41.0	34.0	41.0
28	37.662848108691875	40.0	35.0	41.0	33.0	41.0
29	37.63933554350647	40.0	35.0	41.0	33.0	41.0
30	37.44929005824614	40.0	35.0	41.0	33.0	41.0
31	37.31586434412927	40.0	35.0	41.0	33.0	41.0
32	37.12722512506092	40.0	35.0	41.0	32.0	41.0
33	36.9072343916871	40.0	35.0	41.0	31.0	41.0
34	36.684074848989106	40.0	35.0	41.0	31.0	41.0
35	36.54935677452894	40.0	35.0	41.0	30.0	41.0
36	36.40206195279159	40.0	35.0	41.0	30.0	41.0
37	36.373190674108514	40.0	35.0	41.0	30.0	41.0
38	36.249989080454355	39.0	35.0	41.0	30.0	41.0
39	36.19046342551714	39.0	35.0	41.0	29.0	41.0
40	36.10132567566294	39.0	35.0	41.0	29.0	41.0
41	35.95998307684534	39.0	35.0	41.0	28.0	41.0
42	35.95889026584597	39.0	35.0	41.0	28.0	41.0
43	35.922764983115385	39.0	35.0	41.0	28.0	41.0
44	35.782284814294925	39.0	35.0	41.0	27.0	41.0
45	35.71960234012286	39.0	35.0	41.0	27.0	41.0
46	35.614525479368055	39.0	35.0	41.0	26.0	41.0
47	35.54130371667076	39.0	35.0	41.0	26.0	41.0
48	35.47503620578762	38.0	35.0	40.0	26.0	41.0
49	35.39330147966267	38.0	35.0	40.0	26.0	41.0
50	35.3045525512769	38.0	35.0	40.0	26.0	41.0
51	34.19166586019042	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	24.0
10	24.0
11	15.0
12	23.0
13	28.0
14	29.0
15	51.0
16	79.0
17	189.0
18	366.0
19	680.0
20	1296.0
21	2043.0
22	3056.0
23	4540.0
24	6858.0
25	10770.0
26	15663.0
27	18711.0
28	18953.0
29	18518.0
30	19651.0
31	22791.0
32	28267.0
33	38950.0
34	64368.0
35	82240.0
36	89817.0
37	133110.0
38	223678.0
39	362639.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56555538522016	19.237841407088368	22.473538301055726	17.723064906635745
2	36.3754473802083	24.773151791961673	22.523896676261593	16.32750415156843
3	29.309088230785243	25.13019952365088	30.189931579411645	15.370780666152234
4	26.507346927239855	23.908323776946656	31.869143590740567	17.715185705072923
5	24.309649195679114	31.736910034077546	26.316875793808148	17.636564976435192
6	25.129856949669886	33.096586164635916	27.70918209605603	14.064374789638165
7	75.00383254641235	5.258339321241043	15.144510551706833	4.593317580639774
8	75.76237698382451	6.235531602021529	13.508120288001946	4.493971126152012
9	69.25878124167653	7.110551192971067	15.237690674536733	8.392976890815678
10	36.129821835836836	28.472950786678325	21.25662987707589	14.140597500408948
11	27.49575850589784	23.376049453979896	30.95943838421556	18.168753655906702
12	25.158290590092246	21.209440311194207	33.28012017495253	20.352148923761018
13	23.325519791783535	21.460803969747293	35.859017103862435	19.354659134606738
14	19.63497029455367	25.66949661322798	33.47538734411813	21.220145748100215
15	19.083940046127587	24.662586039596412	36.56763138354498	19.685842530731026
16	22.310301799112906	24.51793417612242	33.407985913357905	19.763778111406772
17	21.367109985945902	24.986061521148375	32.49742427188041	21.149404221025307
18	21.979204046483865	24.956000654316306	31.822896103306608	21.241899195893225
19	22.403053704466565	26.40500295041841	30.14625339683513	21.045689948279893
20	25.02006198876186	25.07418867775864	30.98470321531374	18.92104611816576
21	23.605574021244728	26.50580534432539	30.696084636327743	19.19253599810214
22	22.59104117653608	23.34650244811931	31.756693681479852	22.305762693864757
23	22.23596324523758	25.792823246385204	31.82024115495392	20.1509723534233
24	22.28529389850047	24.159173574528253	31.735625381648823	21.81990714532245
25	22.086172772048705	26.496213272857606	29.79297397893684	21.62463997615685
26	21.238816030064292	25.56141452222492	30.855809754965396	22.343959692745397
27	23.021913601129125	25.185782152066878	29.901141713435152	21.891162533368846
28	20.436764697066113	26.49818307324831	31.335156397868847	21.72989583181673
29	22.703662372787292	23.890081712458816	30.269323099506607	23.13693281524728
30	23.178041692966357	23.67117693860475	31.105888761089762	22.044892607339133
31	23.08152147382178	23.83312878811885	30.73265440879867	22.3526953292607
32	23.08289176974575	24.651366741718917	30.967831446749873	21.29791004178546
33	22.166335083600895	24.492669345024243	29.68591960987675	23.65507596149811
34	20.960303383517566	24.975013510261377	32.03015336180694	22.034529744414115
35	21.565802894921426	25.693562435392685	30.186934057077963	22.553700612607923
36	20.316606873233066	27.19823300340604	29.15133291253829	23.333827210822598
37	21.63097759480521	26.269600584431213	30.614038167880093	21.48538365288349
38	20.707826359526255	26.17325165227713	29.52525241279137	23.593669575405244
39	22.12933709365373	24.710375109944838	29.75023787480805	23.41004992159338
40	22.099533157307402	25.182013838275964	30.683152468545284	22.03530053587135
41	19.86209684395156	26.722654674293505	30.1387167692533	23.27653171250164
42	21.41027430755093	26.866878320291253	29.577238014406948	22.145609357750864
43	22.69972277200588	24.851601233608907	28.88917817358395	23.559497820801266
44	22.276900835966156	25.275707822077354	29.193726442686092	23.253664899270404
45	21.798753201996178	23.731041741783148	29.507609852770265	24.962595203450405
46	22.341732961868946	24.985290729691144	28.78897528414371	23.884001024296204
47	21.084315164636774	24.72938796588991	31.660087818840026	22.52620905063329
48	21.367109985945902	24.372597164686447	30.283197345736795	23.977095503630856
49	20.457062205439904	24.27838931991357	31.568192348438846	23.69635612620768
50	19.923845804025415	23.837325319386004	32.05755928028633	24.181269596302258
51	20.0287590857043	23.50108895704208	30.50364370250533	25.966508254748287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	996.0
2	1538.0
3	13620.0
4	25702.0
5	17159.5
6	8617.0
7	8160.0
8	7703.0
9	7656.5
10	7610.0
11	7349.5
12	7089.0
13	6796.5
14	6504.0
15	6219.0
16	5934.0
17	5617.5
18	5301.0
19	5110.5
20	4920.0
21	4847.0
22	4774.0
23	5357.5
24	5941.0
25	6655.5
26	8697.5
27	10025.0
28	11865.0
29	13705.0
30	16053.5
31	18402.0
32	20896.5
33	23391.0
34	25595.0
35	27799.0
36	30994.0
37	34189.0
38	37376.0
39	40563.0
40	45297.5
41	50032.0
42	53725.0
43	57418.0
44	62978.0
45	68538.0
46	90844.5
47	113151.0
48	105653.5
49	98156.0
50	97716.5
51	97277.0
52	87697.5
53	78118.0
54	71427.5
55	64737.0
56	60308.5
57	55880.0
58	53154.5
59	50429.0
60	48653.5
61	46878.0
62	44058.0
63	41238.0
64	34534.0
65	27830.0
66	22172.5
67	16515.0
68	14348.5
69	12182.0
70	10009.0
71	7836.0
72	6946.5
73	6057.0
74	4751.0
75	2733.0
76	2021.0
77	1673.5
78	1326.0
79	957.0
80	588.0
81	404.0
82	220.0
83	160.5
84	101.0
85	79.0
86	57.0
87	49.0
88	41.0
89	28.0
90	15.0
91	12.0
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1167631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.105458283432053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4837694289811	22.546408661990792
2	9.205191037674625	5.726633716268213
3	3.671044293644157	3.425685453977388
4	1.9738253332424196	2.455869662478138
5	1.3153403284926823	2.0457131858222475
6	1.048673458130909	1.9571681102900054
7	0.8037626794302453	1.7500984546357945
8	0.7778396595274262	1.935604726450348
9	0.6763291108978791	1.89337742504142
>10	7.921523980357055	47.469727192803894
>50	0.08318320192241264	1.7131224861161827
>100	0.0328851820777742	1.8193161145536627
>500	0.0027634606755550743	0.6797614847010194
>1k	0.0030398067431105817	2.1182805092927692
>5k	5.526921351110149E-4	1.2632492133492892
>10k+	2.763460675555074E-4	1.1999836022289112
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13960	1.1955831936630665	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	9629	0.8246612157436723	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	5067	0.43395559042197407	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	4834	0.4140006560291736	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	3128	0.26789285313596506	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	2751	0.2356052554274424	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	2469	0.21145378976748647	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2332	0.19972063091850079	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	2281	0.1953528126608492	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	1874	0.16049591009488443	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1438	0.1231553461667256	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1295	0.11090832634625152	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	1177	0.10080239390697918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.13860971488424E-4	0.0	0.0	0.08016231155219414	0.0
2	5.13860971488424E-4	0.0	0.0	0.45271151588130154	0.0
3	5.13860971488424E-4	0.0	0.0	0.8180666666095711	0.0
4	5.13860971488424E-4	0.0	0.0	1.3781751255319532	0.0
5	5.13860971488424E-4	0.0	0.0	2.963008005097501	0.0
6	5.13860971488424E-4	0.0	0.0	4.172551088486003	0.0
7	5.13860971488424E-4	0.0	0.0	5.105208751737492	0.0
8	5.13860971488424E-4	0.0	0.0	6.285461759751154	0.0
9	5.13860971488424E-4	0.0	0.0	6.953566666181353	0.0
10	5.13860971488424E-4	0.0	0.0	8.265368082896051	0.0
11	5.13860971488424E-4	0.0	0.0	9.097737213212051	0.0
12	5.13860971488424E-4	0.0	0.0	10.21906749649504	0.0
13	5.995044667364947E-4	0.0	0.0	10.619964697751259	0.0
14	5.995044667364947E-4	0.0	0.0	10.857539753569407	0.0
15	5.995044667364947E-4	0.0	0.0	11.162858814128779	0.0
16	5.995044667364947E-4	0.0	0.0	11.520848624265714	0.0
17	6.851479619845653E-4	0.0	0.0	11.910183953663443	0.0
18	6.851479619845653E-4	0.0	0.0	12.344567761561658	0.0
19	6.851479619845653E-4	0.0	0.0	12.714290730547578	0.0
20	6.851479619845653E-4	0.0	0.0	13.030657801993952	0.0
21	6.851479619845653E-4	0.0	0.0	13.359357536756047	0.0
22	6.851479619845653E-4	0.0	0.0	13.759398303059784	0.0
23	6.851479619845653E-4	0.0	0.0	14.119015339606435	0.0
24	6.851479619845653E-4	0.0	0.0	14.436752706976776	0.0
25	6.851479619845653E-4	0.0	0.0	14.728368808296457	0.0
26	6.851479619845653E-4	0.0	0.0	15.002256706099786	0.0
27	6.851479619845653E-4	0.0	0.0	15.288905484695078	0.0
28	6.851479619845653E-4	0.0	0.0	15.578723072614551	0.0
29	6.851479619845653E-4	0.0	0.0	15.87487827918238	0.0
30	7.70791457232636E-4	0.0	0.0	16.241775012825112	0.0
31	7.70791457232636E-4	0.0	0.0	16.57261583496841	0.0
32	7.70791457232636E-4	0.0	0.0	16.933945741420022	0.0
33	7.70791457232636E-4	0.0	0.0	17.250227169371147	0.0
34	7.70791457232636E-4	0.0	0.0	17.564538796931565	0.0
35	7.70791457232636E-4	0.0	0.0	17.87414003225334	0.0
36	7.70791457232636E-4	0.0	0.0	18.200698679634232	0.0
37	7.70791457232636E-4	0.0	0.0	18.556975619866208	0.0
38	7.70791457232636E-4	0.0	0.0	18.903317914649406	0.0
39	8.564349524807067E-4	0.0	0.0	19.261735942262582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCG	20	7.0333324E-4	45.0	1
CGTTTTT	7480	0.0	43.014706	1
CGTTATT	570	0.0	41.05263	1
TACGGCT	655	0.0	40.534355	7
TCGTTAG	35	6.248374E-6	38.57143	1
GCGAACC	35	6.248374E-6	38.57143	33
CATACGA	100	0.0	38.25	18
ACGGGTA	90	0.0	37.5	5
GTACGAG	30	1.1399332E-4	37.499996	1
TAATACG	85	0.0	37.058826	4
TCGTCCC	85	0.0	37.058826	38
ACTTCGT	25	0.0021071224	36.0	37
CGTTAGG	150	0.0	36.0	2
CCCGTAA	25	0.0021071224	36.0	23
GTTTTTT	9155	0.0	35.783726	2
ACAACGA	380	0.0	35.526318	13
ATAGGGC	370	0.0	35.27027	4
CGTTTCT	390	0.0	34.615383	1
CGTATGG	105	0.0	34.285713	2
GGGCGAT	2195	0.0	34.2369	7
>>END_MODULE