##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550924_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285233
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735889606041376	31.0	31.0	34.0	30.0	34.0
2	31.798701412529407	33.0	31.0	34.0	30.0	34.0
3	31.847461548979254	33.0	31.0	34.0	30.0	34.0
4	35.57226548120309	37.0	35.0	37.0	33.0	37.0
5	33.624857572581014	37.0	35.0	37.0	32.0	37.0
6	34.54348900723269	37.0	35.0	37.0	30.0	37.0
7	35.58464834012895	37.0	35.0	37.0	32.0	37.0
8	35.52065504342064	37.0	35.0	37.0	33.0	37.0
9	37.36557130486304	39.0	37.0	39.0	34.0	39.0
10	37.07887586639695	39.0	37.0	39.0	33.0	39.0
11	37.00565151998541	39.0	37.0	39.0	33.0	39.0
12	36.820438027857925	39.0	35.0	39.0	33.0	39.0
13	36.63898637254455	39.0	35.0	39.0	33.0	39.0
14	37.568384443595235	39.0	36.0	41.0	33.0	41.0
15	37.64767050095887	39.0	36.0	41.0	33.0	41.0
16	37.756213341373545	39.0	36.0	41.0	33.0	41.0
17	37.70873636640922	39.0	36.0	41.0	33.0	41.0
18	37.74730132908885	39.0	36.0	41.0	33.0	41.0
19	37.65998674767646	39.0	36.0	41.0	33.0	41.0
20	37.65296792446877	39.0	35.0	41.0	33.0	41.0
21	37.622778570502014	39.0	35.0	41.0	33.0	41.0
22	37.68294341818794	39.0	35.0	41.0	33.0	41.0
23	37.70061318290661	39.0	35.0	41.0	33.0	41.0
24	37.641100433680535	39.0	35.0	41.0	33.0	41.0
25	37.48623055537052	39.0	35.0	41.0	33.0	41.0
26	37.51252835401233	39.0	35.0	41.0	33.0	41.0
27	37.44023657851651	39.0	35.0	41.0	33.0	41.0
28	37.36597097811263	39.0	35.0	41.0	33.0	41.0
29	37.36079275539647	39.0	35.0	41.0	33.0	41.0
30	37.160037583309084	39.0	35.0	41.0	32.0	41.0
31	37.117696760192544	39.0	35.0	41.0	32.0	41.0
32	37.035879438914854	39.0	35.0	41.0	32.0	41.0
33	36.92949974231593	39.0	35.0	41.0	32.0	41.0
34	36.83576935347593	39.0	35.0	41.0	31.0	41.0
35	36.76414019415706	39.0	35.0	41.0	31.0	41.0
36	36.67571774654405	39.0	35.0	40.0	31.0	41.0
37	36.52078476193147	39.0	35.0	40.0	31.0	41.0
38	36.494767435745516	39.0	35.0	40.0	31.0	41.0
39	36.45436888438575	39.0	35.0	40.0	31.0	41.0
40	36.41400889798866	38.0	35.0	40.0	31.0	41.0
41	36.27137813647089	38.0	35.0	40.0	30.0	41.0
42	36.35314286916311	38.0	35.0	40.0	31.0	41.0
43	36.24145172543149	38.0	35.0	40.0	31.0	41.0
44	36.10543660796612	38.0	35.0	40.0	30.0	41.0
45	36.07851475811004	38.0	35.0	40.0	30.0	41.0
46	35.99755988963409	38.0	35.0	40.0	30.0	41.0
47	35.85227515750282	38.0	35.0	40.0	30.0	41.0
48	35.807641472059686	38.0	35.0	40.0	29.0	41.0
49	35.776975314917976	38.0	35.0	40.0	29.0	41.0
50	35.647120073764256	38.0	35.0	40.0	29.0	41.0
51	34.23955503044879	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	8.0
10	8.0
11	3.0
12	4.0
13	6.0
14	5.0
15	9.0
16	12.0
17	32.0
18	66.0
19	133.0
20	279.0
21	444.0
22	672.0
23	916.0
24	1267.0
25	1829.0
26	2258.0
27	2662.0
28	3296.0
29	3851.0
30	4941.0
31	6464.0
32	8845.0
33	12935.0
34	22788.0
35	28865.0
36	23404.0
37	34098.0
38	55997.0
39	69106.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92409012982369	20.610869008845402	22.810824834433603	12.654216026897307
2	29.236448798000232	29.074475954745772	24.546248155017125	17.142827092236875
3	28.765605662738885	28.756840898493515	26.488870502361227	15.988682936406375
4	28.924423190865014	23.26799493747217	31.00062054530857	16.806961326354244
5	26.509905936550123	31.321761507258977	23.75180992381667	18.416522632374235
6	24.8996434493905	35.3349717599296	25.048293850992	14.717090939687905
7	73.07429364764947	5.808935151262301	15.334831523701675	5.781939677386558
8	71.04121893329314	9.224037891828786	11.570540575599598	8.164202599278484
9	65.24981331052157	7.3964092513839566	14.227666504226368	13.126110933868102
10	43.499875540347716	19.31122976654174	20.977937335441553	16.210957357668992
11	33.224767120214	22.17204881623094	25.228847994446646	19.374336069108413
12	29.380541522194136	18.356221054366078	30.61251678452353	21.650720638916255
13	24.192852860643754	24.963801523666618	32.734290913043026	18.109054702646606
14	20.129508156489607	29.595102950920825	29.002254297363912	21.273134595225656
15	16.69757706857201	23.969526667671694	40.20116886895976	19.131727394796535
16	18.828115961336874	23.534794361101273	31.80592708417329	25.831162593388562
17	18.673505520048522	24.336594994267845	30.343964408045355	26.645935077638285
18	20.643473931838184	23.51551187976146	32.944645254931935	22.896368933468427
19	22.08229763035834	25.81047774976949	27.61952509001413	24.487699529858045
20	23.161064813678642	26.630158501996615	30.571497687855192	19.637278996469554
21	22.06091160559963	28.96368933468428	28.7235347943611	20.25186426535499
22	18.28049349128607	24.59498024422139	28.794354089463702	28.330172175028835
23	18.854410254072988	27.387784723366508	29.032405086367984	24.725399936192517
24	23.50429298152738	22.66392738568118	28.417469226912733	25.414310405878705
25	20.10005854862516	28.28424481038309	26.499738810025487	25.115957830966263
26	20.280612692079806	25.589255100216313	29.03415803921706	25.09597416848682
27	22.708101797477852	26.087093709353404	29.283778524925236	21.92102596824351
28	19.849736881777353	24.392338894868406	32.05344402646257	23.704480196891666
29	24.339399718826364	21.44983224241234	30.406720120042213	23.804047918719082
30	24.990446406972545	24.569387132624907	30.582015404949637	19.85815105545291
31	28.82590724074704	25.38170548288592	23.322336475793474	22.470050800573567
32	28.92056669459705	25.543678326140384	26.202087416252677	19.33366756300989
33	26.83665634761756	24.65983949963714	24.411621376208224	24.091882776537076
34	26.4103382147227	25.309133234934244	27.319770152822432	20.960758397520625
35	25.26951650054517	22.577331514936912	28.728793652908323	23.424358331609596
36	30.604453201417787	26.277113798193056	25.371888946931104	17.74654405345805
37	23.168427215644755	25.74561849435374	33.63671103974645	17.449243250255055
38	27.237731959485757	26.510607117689748	24.215991838251536	22.035669084572962
39	24.810593444657524	25.236210396412755	29.29254328917061	20.660652869759108
40	28.276882408416977	22.19308425041983	28.70004522618351	20.829988114979685
41	22.284588389141508	28.32806863160995	24.376912909796552	25.010430069451996
42	25.960881104220057	22.985769528771215	28.990334217990206	22.063015149018522
43	26.54531558410142	23.514109517482197	27.22581188011205	22.714763018304335
44	23.768988861737597	22.311583863017255	29.17369308600337	24.745734189241777
45	23.190865012112905	21.67280784481459	27.837241833869154	27.299085309203353
46	28.149267441004373	23.412788842805707	27.480340633797635	20.95760308239229
47	20.754260551899677	22.92827267532158	34.13384846774392	22.18361830503483
48	23.560036882127942	22.4118527659843	28.408704462667362	25.619405889220392
49	22.53035237858172	19.790837666048457	34.06583389719983	23.612976058169988
50	22.93142799044991	20.063246538794598	31.133143780698585	25.8721816900569
51	21.921727149383134	20.911675717746544	25.95667401738228	31.209923115488042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	355.0
2	454.0
3	992.0
4	1530.0
5	1080.5
6	631.0
7	584.0
8	537.0
9	568.0
10	599.0
11	655.5
12	712.0
13	701.0
14	690.0
15	639.0
16	588.0
17	590.0
18	592.0
19	568.0
20	544.0
21	660.0
22	776.0
23	796.0
24	816.0
25	840.0
26	1041.5
27	1219.0
28	1572.5
29	1926.0
30	2090.5
31	2255.0
32	2916.0
33	3577.0
34	3529.0
35	3481.0
36	3823.5
37	4166.0
38	5042.5
39	5919.0
40	7870.0
41	9821.0
42	12609.0
43	15397.0
44	18275.5
45	21154.0
46	31917.0
47	42680.0
48	40491.0
49	38302.0
50	37299.5
51	36297.0
52	29218.5
53	22140.0
54	18933.5
55	15727.0
56	13703.5
57	11680.0
58	10732.0
59	9784.0
60	9269.5
61	8755.0
62	7890.5
63	7026.0
64	5759.0
65	4492.0
66	3963.0
67	3434.0
68	2884.5
69	2335.0
70	2011.0
71	1687.0
72	1374.0
73	1061.0
74	880.0
75	530.0
76	361.0
77	236.5
78	112.0
79	102.5
80	93.0
81	59.5
82	26.0
83	28.5
84	31.0
85	16.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.16762085733418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91274245547275	23.52006955716905
2	12.44225992283706	8.253603194581272
3	4.828497436710533	4.804493168742747
4	2.51678029702447	3.3390245869166613
5	1.4946355900850905	2.4786753285910117
6	0.8889593573278368	1.769080015285749
7	0.6426721632049046	1.4921134651320147
8	0.4978595211669573	1.3210252670623666
9	0.4302098197769674	1.284213257231807
>10	5.113894614449553	37.55245711400855
>50	0.1691242534749749	3.539211802280942
>100	0.04862322287405528	2.798413928262158
>500	0.007399186089530152	1.7392798168514862
>1k	0.006342159505311559	6.108339497884186
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	4228	1.482296929177199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	3676	1.2887709346394	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	3528	1.2368835303068018	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2649	0.9287144194395459	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2207	0.7737533875813808	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1135	0.39792029673985824	Illumina Single End Adapter 1 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	875	0.30676674858799646	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.2885360389576241	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT	776	0.27205828217632605	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	673	0.23594745348539614	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	640	0.22437796468150598	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	635	0.22262501183243172	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTA	539	0.18896831713020584	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	484	0.1696858357903889	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	429	0.15040335445057199	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	321	0.11253957291056786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5059056981485314E-4	0.0	0.0	0.424915770615602	0.0
2	3.5059056981485314E-4	0.0	0.0	2.6473093926719558	0.0
3	3.5059056981485314E-4	0.0	0.0	3.3947684875172226	0.0
4	3.5059056981485314E-4	0.0	0.0	4.443034291263634	0.0
5	3.5059056981485314E-4	0.0	0.0	7.888989001973825	0.0
6	3.5059056981485314E-4	0.0	0.0	9.229997931515639	0.0
7	3.5059056981485314E-4	0.0	0.0	10.957007078423604	0.0
8	3.5059056981485314E-4	0.0	0.0	13.663215686824456	0.0
9	3.5059056981485314E-4	0.0	0.0	14.599292508230114	0.0
10	3.5059056981485314E-4	0.0	0.0	17.81000094659454	0.0
11	3.5059056981485314E-4	0.0	0.0	20.1954191836148	0.0
12	3.5059056981485314E-4	0.0	0.0	23.66451287193277	0.0
13	3.5059056981485314E-4	0.0	0.0	24.472974725925823	0.0
14	3.5059056981485314E-4	0.0	0.0	24.820059390042527	0.0
15	3.5059056981485314E-4	0.0	0.0	25.512826355996676	0.0
16	3.5059056981485314E-4	0.0	0.0	26.607019524388832	0.0
17	3.5059056981485314E-4	0.0	0.0	27.907359947832123	0.0
18	3.5059056981485314E-4	0.0	0.0	29.34548246521265	3.5059056981485314E-4
19	3.5059056981485314E-4	0.0	0.0	30.452296894118142	3.5059056981485314E-4
20	3.5059056981485314E-4	0.0	0.0	31.251993983865823	3.5059056981485314E-4
21	3.5059056981485314E-4	0.0	0.0	32.10427965908573	3.5059056981485314E-4
22	3.5059056981485314E-4	0.0	0.0	33.0501730164462	3.5059056981485314E-4
23	3.5059056981485314E-4	0.0	0.0	33.8880844783037	3.5059056981485314E-4
24	3.5059056981485314E-4	0.0	0.0	34.57419022343137	3.5059056981485314E-4
25	7.011811396297063E-4	0.0	0.0	35.146354033369214	3.5059056981485314E-4
26	7.011811396297063E-4	0.0	0.0	35.70519540165409	3.5059056981485314E-4
27	7.011811396297063E-4	0.0	0.0	36.298044055211	3.5059056981485314E-4
28	7.011811396297063E-4	0.0	0.0	36.86424782546199	3.5059056981485314E-4
29	7.011811396297063E-4	0.0	0.0	37.449734077052796	3.5059056981485314E-4
30	7.011811396297063E-4	0.0	0.0	38.11340202571231	3.5059056981485314E-4
31	7.011811396297063E-4	0.0	0.0	38.68381288280108	3.5059056981485314E-4
32	7.011811396297063E-4	0.0	0.0	39.25176960590114	3.5059056981485314E-4
33	7.011811396297063E-4	0.0	0.0	39.80640388734824	3.5059056981485314E-4
34	7.011811396297063E-4	0.0	0.0	40.367348799052	3.5059056981485314E-4
35	0.0010517717094445593	0.0	0.0	40.936006703291696	3.5059056981485314E-4
36	0.0010517717094445593	0.0	0.0	41.519389411463614	3.5059056981485314E-4
37	0.0010517717094445593	0.0	0.0	42.04807999074441	3.5059056981485314E-4
38	0.0010517717094445593	0.0	0.0	42.58869064939891	3.5059056981485314E-4
39	0.0010517717094445593	0.0	0.0	43.227466667601576	3.5059056981485314E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAGG	45	3.8198777E-10	45.000004	2
GTCTTAC	45	3.8198777E-10	45.000004	31
TTACACG	45	3.8198777E-10	45.000004	34
TAATACG	40	6.7848305E-9	45.0	4
AACCGGG	20	7.0243585E-4	45.0	2
AACCGCT	20	7.0243585E-4	45.0	13
AATCCAT	20	7.0243585E-4	45.0	33
GGTGTCG	20	7.0243585E-4	45.0	9
GTCGAGC	20	7.0243585E-4	45.0	23
TTGTGCG	20	7.0243585E-4	45.0	1
GATCGAA	20	7.0243585E-4	45.0	42
CCGATCG	20	7.0243585E-4	45.0	40
CGCATGG	35	1.207627E-7	45.0	2
ATTACGG	35	1.207627E-7	45.0	2
CCTAATC	20	7.0243585E-4	45.0	12
GGATAAT	20	7.0243585E-4	45.0	7
ATCAATT	20	7.0243585E-4	45.0	29
GTAATGT	20	7.0243585E-4	45.0	32
CTGCGTA	20	7.0243585E-4	45.0	35
AGACGAA	20	7.0243585E-4	45.0	24
>>END_MODULE