SRR3550923_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561221
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	3238	0.5769563148919944	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	3238	0.5769563148919944	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	2634	0.46933382749398184	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2322	0.41374075453341913	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1373	0.24464515761170735	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.21399769431293555	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	1051	0.18727025538958805	Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	757	0.13488447509982698	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	598	0.10655338984107865	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAG	35	1.2104465E-7	45.000004	1
GAGTACG	25	3.8882445E-5	45.000004	1
TCGCTAC	25	3.8882445E-5	45.000004	2
TGCGTAA	25	3.8882445E-5	45.000004	27
CTAACGG	40	6.8048394E-9	45.0	2
CCGAGTA	20	7.0301996E-4	45.0	23
GTCCGCC	20	7.0301996E-4	45.0	10
ACTCGTT	20	7.0301996E-4	45.0	32
AACGGGA	200	0.0	41.625004	4
CGGGATA	60	3.6379788E-12	41.250004	6
ACGTAGG	60	3.6379788E-12	41.250004	2
CACGACC	160	0.0	40.78125	27
CACGTGA	155	0.0	40.645164	43
GTTACGG	50	1.0786607E-9	40.500004	2
CGAAAGG	95	0.0	40.263157	2
GTAACGG	85	0.0	39.705883	2
CGCATCG	40	3.4540062E-7	39.375	21
TAACGCC	40	3.4540062E-7	39.375	12
TAACGAG	40	3.4540062E-7	39.375	1
CGGGCTA	40	3.4540062E-7	39.375	6