FastQCFastQC Report
Sat 18 Jun 2016
SRR3550921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585067
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC29630.506437724226456TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC23150.39568117839495304TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT14330.24492921323540723TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC9150.15639234480837239No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC9130.1560505036175344TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC8080.13810384109854085No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.13451450859474212No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG7610.13007057311384848No Hit
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC7070.12084086096122325TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG253.8884486E-545.0000041
CGACATA253.8884486E-545.00000422
TGCGAAT253.8884486E-545.00000427
GCGATAA302.163668E-645.0000049
AGTTACG253.8884486E-545.0000041
CCTATTA253.8884486E-545.00000429
AGGTGCG302.163668E-645.0000041
TGCGTAG302.163668E-645.0000041
AACCGAT207.0304447E-445.029
GAGTAGC207.0304447E-445.028
CACGACA207.0304447E-445.012
CACGAAC207.0304447E-445.017
TCGATCT207.0304447E-445.012
AAAACCG207.0304447E-445.027
TACGAGT207.0304447E-445.013
CGTACCC207.0304447E-445.012
CGTAAGA207.0304447E-445.012
ATAACGG207.0304447E-445.02
CACGTAG207.0304447E-445.019
CCACGAA207.0304447E-445.016
Produced by FastQC (version 0.11.5)