##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585067
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36611704300533	31.0	31.0	33.0	28.0	34.0
2	31.281948904997204	31.0	31.0	34.0	28.0	34.0
3	31.2013991559941	31.0	31.0	34.0	28.0	34.0
4	35.064134535019065	37.0	35.0	37.0	32.0	37.0
5	35.3622679111965	37.0	35.0	37.0	33.0	37.0
6	35.30343875145923	37.0	35.0	37.0	33.0	37.0
7	35.66303688295528	37.0	35.0	37.0	35.0	37.0
8	35.44373892220891	37.0	35.0	37.0	33.0	37.0
9	37.32972462982872	39.0	37.0	39.0	34.0	39.0
10	36.65831092849195	38.0	35.0	39.0	32.0	39.0
11	36.74481555103945	39.0	37.0	39.0	33.0	39.0
12	36.779291944341416	39.0	35.0	39.0	33.0	39.0
13	36.78814392197817	39.0	35.0	39.0	33.0	39.0
14	37.67859749396223	39.0	36.0	41.0	33.0	41.0
15	37.859720339721775	40.0	36.0	41.0	33.0	41.0
16	37.864465095450605	39.0	36.0	41.0	33.0	41.0
17	37.76617549784896	39.0	36.0	41.0	33.0	41.0
18	37.72999673541663	39.0	36.0	41.0	33.0	41.0
19	37.60112602488262	39.0	36.0	41.0	33.0	41.0
20	37.80259867673275	39.0	36.0	41.0	33.0	41.0
21	37.62947320563286	39.0	35.0	41.0	33.0	41.0
22	37.81339573074537	39.0	36.0	41.0	33.0	41.0
23	37.900110585625235	40.0	36.0	41.0	34.0	41.0
24	37.81264026171362	40.0	36.0	41.0	34.0	41.0
25	37.47916221560949	39.0	35.0	41.0	33.0	41.0
26	37.62968001955332	39.0	35.0	41.0	33.0	41.0
27	37.72460931824902	39.0	35.0	41.0	34.0	41.0
28	37.66264034717391	40.0	35.0	41.0	33.0	41.0
29	37.69752865911084	40.0	36.0	41.0	33.0	41.0
30	37.41751286604782	39.0	35.0	41.0	33.0	41.0
31	37.34594328512803	39.0	35.0	41.0	33.0	41.0
32	37.35459186725623	39.0	35.0	41.0	33.0	41.0
33	37.376522688854436	39.0	35.0	41.0	33.0	41.0
34	37.32323477482066	39.0	35.0	41.0	33.0	41.0
35	37.29028983005365	40.0	35.0	41.0	33.0	41.0
36	37.21823483464287	39.0	35.0	41.0	33.0	41.0
37	37.22550579677199	39.0	35.0	41.0	33.0	41.0
38	37.06925189764591	39.0	35.0	41.0	32.0	41.0
39	37.064566964125476	39.0	35.0	41.0	33.0	41.0
40	36.949436560257205	39.0	35.0	41.0	32.0	41.0
41	36.830771518475665	39.0	35.0	41.0	32.0	41.0
42	36.84189674003149	39.0	35.0	41.0	32.0	41.0
43	36.8095551449663	39.0	35.0	41.0	32.0	41.0
44	36.69506398412489	39.0	35.0	41.0	32.0	41.0
45	36.62909547111698	39.0	35.0	41.0	31.0	41.0
46	36.50542587430157	39.0	35.0	40.0	31.0	41.0
47	36.502747548571364	39.0	35.0	40.0	31.0	41.0
48	36.40766954895764	38.0	35.0	40.0	31.0	41.0
49	36.428528698422575	38.0	35.0	40.0	31.0	41.0
50	36.3079100342354	38.0	35.0	40.0	31.0	41.0
51	35.190815752725754	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	22.0
10	23.0
11	23.0
12	13.0
13	12.0
14	21.0
15	31.0
16	50.0
17	76.0
18	146.0
19	277.0
20	470.0
21	734.0
22	1122.0
23	1484.0
24	2238.0
25	3028.0
26	3977.0
27	4849.0
28	5761.0
29	6862.0
30	8749.0
31	11354.0
32	15442.0
33	23122.0
34	46730.0
35	49099.0
36	45982.0
37	71480.0
38	130346.0
39	151501.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15608297853066	21.015029047955192	24.10168408062666	11.727203892887482
2	32.243144802219234	23.716770899743107	25.25009956124683	18.789984736790828
3	33.44352014384677	22.725431446312985	26.54362662737772	17.287421782462523
4	30.26268786309944	25.75140966761072	24.498390782594132	19.48751168669571
5	25.71739646912234	29.37116603739401	24.474120058044633	20.437317435439017
6	26.654383173209222	33.66981901218151	23.697456872460762	15.978340942148506
7	80.03117591660443	5.747888703345087	9.317052576884356	4.903882803166133
8	80.64016599808227	5.078221810493499	8.359384480751778	5.922227710672453
9	74.73947428243261	7.215412935612503	11.053434905745837	6.99167787620905
10	42.80996877280722	25.576728819092516	17.35390989408051	14.259392514019762
11	33.427624528472805	24.27568124676319	23.113079356723247	19.183614868040756
12	31.284622103109562	22.55075059779478	26.466712359439175	19.69791493965648
13	26.077697084265562	25.089434201552983	28.47725132335271	20.355617390828744
14	21.20081973517563	28.04943707301899	28.945060993014476	21.80468219879091
15	20.677289951407275	25.896179411930596	34.923008817793516	18.50352181886861
16	24.333281487419388	24.683326866837476	30.900050763416843	20.083340882326297
17	23.007792269945153	24.926546874118692	27.613589554700575	24.452071301235584
18	24.14902908555772	25.036619737568515	29.66959339699556	21.1447577798782
19	25.205489285842475	27.830829631478103	25.542886541199554	21.420794541479864
20	26.853676587467763	27.036048862779815	26.645153461056598	19.465121088695824
21	25.408884794391067	27.446087371189964	27.327981239755445	19.817046594663516
22	23.656606850155622	24.892191834439476	27.407630237220694	24.043571078184208
23	23.174439850478663	25.92814156327395	27.92739976788983	22.970018818357556
24	25.478449476726595	23.139230207822354	28.044822216942677	23.337498098508373
25	22.87276499956415	26.19922162760846	25.955659779136408	24.97235359369098
26	21.120999817114964	26.49115400458409	27.739728954119787	24.648117224181163
27	22.29642075181133	25.92933800744188	28.590742598710918	23.183498642035868
28	20.27682299634059	27.574619658945043	28.710216094908787	23.43834124980558
29	22.31129084361278	26.471839977301748	27.118774430962606	24.098094748122865
30	23.280581540233854	24.05502275807728	28.569377524283546	24.095018177405322
31	24.16936863641258	26.487564672080293	25.40546638248269	23.937600309024436
32	24.354304720655925	26.832311513040384	26.160251731852934	22.653132034450753
33	23.66395643575864	25.241895372666722	27.27909794946562	23.815050242109024
34	21.75374786135605	25.68680168254234	28.281034479811712	24.278415976289892
35	19.417605163169345	26.120427233120306	28.472978308467233	25.98898929524311
36	22.012350722224976	26.76787444856743	28.218306621292943	23.001468207914648
37	22.7264569698855	27.150736582305957	28.011492700835973	22.11131374697257
38	21.85869310694331	27.77528043796693	25.896692173716858	24.469334281372905
39	23.005228461013864	24.573937685769323	27.10817735404663	25.31265649917018
40	23.029157344372525	22.729191699412205	28.818750672999844	25.422900283215426
41	19.349920607383428	24.915778876607295	28.740981802084203	26.993318713925074
42	21.990301965415927	24.12065626671817	29.017531325472124	24.871510442393777
43	23.2593873864019	23.431846267179658	28.556216638436283	24.752549707982162
44	21.936291057263528	24.241326207083976	27.00357394965021	26.818808786002286
45	22.135413550926646	23.276992207730054	25.770381853702222	28.81721238764107
46	22.644927845870644	24.773402020623276	27.403357222335217	25.17831291117086
47	20.37476049751567	24.017078385894266	30.878685688989467	24.7294754276006
48	21.530525563738852	23.046420324509842	29.20674042460094	26.21631368715036
49	22.296078910620494	21.85818034515705	31.304107050987323	24.541633693235134
50	20.287761914447405	22.831402215472757	29.436799546034898	27.44403632404494
51	19.306335855551588	22.75363334455712	27.847579849829163	30.092450950062126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	567.0
2	881.0
3	1444.0
4	2007.0
5	1487.0
6	967.0
7	956.0
8	945.0
9	964.5
10	984.0
11	1055.0
12	1126.0
13	1120.0
14	1114.0
15	1063.5
16	1013.0
17	1113.5
18	1214.0
19	1200.0
20	1186.0
21	1288.0
22	1390.0
23	1420.5
24	1451.0
25	1836.5
26	2798.5
27	3375.0
28	4025.5
29	4676.0
30	5799.0
31	6922.0
32	7744.5
33	8567.0
34	9728.5
35	10890.0
36	11937.5
37	12985.0
38	15462.5
39	17940.0
40	20096.5
41	22253.0
42	26111.5
43	29970.0
44	33974.0
45	37978.0
46	44371.5
47	50765.0
48	56708.5
49	62652.0
50	63243.5
51	63835.0
52	57718.5
53	51602.0
54	45832.5
55	40063.0
56	36452.0
57	32841.0
58	30605.5
59	28370.0
60	26912.5
61	25455.0
62	23234.5
63	21014.0
64	17731.0
65	14448.0
66	11467.0
67	8486.0
68	6674.5
69	4863.0
70	3956.0
71	3049.0
72	2569.0
73	2089.0
74	1701.0
75	1175.0
76	1037.0
77	761.5
78	486.0
79	367.0
80	248.0
81	168.0
82	88.0
83	56.5
84	25.0
85	19.5
86	14.0
87	10.0
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	585067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.400171164536587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.472101956604	20.014197578922275
2	12.094897685228522	6.86994328956095
3	4.654147138521854	3.9653572607687657
4	2.2742055914756563	2.5835131224501926
5	1.2714496681763876	1.8054694101651325
6	0.8499720968860225	1.448361182198587
7	0.5702718639059942	1.1337072981674685
8	0.47860274124509555	1.0873919816941702
9	0.35940655027393204	0.9186486790891765
>10	6.279450791560798	44.831488800997136
>50	0.6368305162401667	10.885837199335068
>100	0.05322040401627224	2.463350275530752
>500	0.0036286639102003798	0.8400673684019598
>1k	0.0018143319551001899	1.152666552718371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2963	0.506437724226456	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2315	0.39568117839495304	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1433	0.24492921323540723	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	915	0.15639234480837239	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	913	0.1560505036175344	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	808	0.13810384109854085	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	787	0.13451450859474212	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	761	0.13007057311384848	No Hit
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	707	0.12084086096122325	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.709205954189862E-4	0.0	0.0	0.2592865432506021	0.0
2	1.709205954189862E-4	0.0	0.0	1.511108984099257	0.0
3	1.709205954189862E-4	0.0	0.0	1.945076375868063	0.0
4	1.709205954189862E-4	0.0	0.0	2.5379999213765263	0.0
5	1.709205954189862E-4	0.0	0.0	4.202595600162033	0.0
6	1.709205954189862E-4	0.0	0.0	5.031047726157859	0.0
7	1.709205954189862E-4	0.0	0.0	5.921373107695358	0.0
8	1.709205954189862E-4	0.0	0.0	7.31198307202423	0.0
9	1.709205954189862E-4	0.0	0.0	7.773810520846331	0.0
10	3.418411908379724E-4	0.0	0.0	8.944787520061805	0.0
11	3.418411908379724E-4	0.0	0.0	10.58784720382452	0.0
12	3.418411908379724E-4	0.0	0.0	11.976577041603782	0.0
13	3.418411908379724E-4	0.0	0.0	12.490193430837836	0.0
14	3.418411908379724E-4	0.0	0.0	12.703331413325312	0.0
15	3.418411908379724E-4	0.0	0.0	13.13200026663613	0.0
16	3.418411908379724E-4	0.0	0.0	13.957204901318994	0.0
17	3.418411908379724E-4	0.0	0.0	14.95247552844375	0.0
18	3.418411908379724E-4	0.0	0.0	15.97406792726303	0.0
19	3.418411908379724E-4	0.0	0.0	16.654844658816852	0.0
20	3.418411908379724E-4	0.0	0.0	17.303146477241068	0.0
21	3.418411908379724E-4	0.0	0.0	18.114848384885832	0.0
22	3.418411908379724E-4	0.0	0.0	18.896981029523115	0.0
23	3.418411908379724E-4	0.0	0.0	19.635528922328554	0.0
24	5.127617862569586E-4	0.0	0.0	20.24127151249344	0.0
25	5.127617862569586E-4	0.0	0.0	20.774031008414422	0.0
26	5.127617862569586E-4	0.0	0.0	21.267649687984452	0.0
27	5.127617862569586E-4	0.0	0.0	21.761268367554486	0.0
28	6.836823816759448E-4	0.0	0.0	22.24446089080396	0.0
29	6.836823816759448E-4	0.0	0.0	22.76987080112192	0.0
30	6.836823816759448E-4	0.0	0.0	23.33168679826413	0.0
31	6.836823816759448E-4	0.0	0.0	23.882051115513267	0.0
32	6.836823816759448E-4	0.0	0.0	24.402504328564078	0.0
33	6.836823816759448E-4	0.0	0.0	24.945689980805618	0.0
34	8.54602977094931E-4	0.0	0.0	25.4478546901466	0.0
35	8.54602977094931E-4	0.0	0.0	25.979246821304226	0.0
36	8.54602977094931E-4	0.0	0.0	26.4957688606604	0.0
37	8.54602977094931E-4	0.0	0.0	27.011094455848646	0.0
38	8.54602977094931E-4	0.0	0.0	27.569492041082473	0.0
39	8.54602977094931E-4	0.0	0.0	28.187711834712946	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	25	3.8884486E-5	45.000004	1
CGACATA	25	3.8884486E-5	45.000004	22
TGCGAAT	25	3.8884486E-5	45.000004	27
GCGATAA	30	2.163668E-6	45.000004	9
AGTTACG	25	3.8884486E-5	45.000004	1
CCTATTA	25	3.8884486E-5	45.000004	29
AGGTGCG	30	2.163668E-6	45.000004	1
TGCGTAG	30	2.163668E-6	45.000004	1
AACCGAT	20	7.0304447E-4	45.0	29
GAGTAGC	20	7.0304447E-4	45.0	28
CACGACA	20	7.0304447E-4	45.0	12
CACGAAC	20	7.0304447E-4	45.0	17
TCGATCT	20	7.0304447E-4	45.0	12
AAAACCG	20	7.0304447E-4	45.0	27
TACGAGT	20	7.0304447E-4	45.0	13
CGTACCC	20	7.0304447E-4	45.0	12
CGTAAGA	20	7.0304447E-4	45.0	12
ATAACGG	20	7.0304447E-4	45.0	2
CACGTAG	20	7.0304447E-4	45.0	19
CCACGAA	20	7.0304447E-4	45.0	16
>>END_MODULE