SRR3550920_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415448
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	2167	0.5216055920355857	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	2157	0.5191985519246693	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	1954	0.4703356376730662	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	1763	0.42436117155456277	No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1558	0.3750168492807764	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	1069	0.25731258785696404	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	849	0.20435770541680306	No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	589	0.14177466253297644	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT	435	0.10470624482486378	No Hit
ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	434	0.10446554081377211	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.0280785E-4	45.000004	12
CGTATGG	40	6.7975634E-9	45.000004	2
TACCAAT	20	7.0280785E-4	45.000004	26
CGCATCG	20	7.0280785E-4	45.000004	21
TTGTCGG	20	7.0280785E-4	45.000004	2
GCGCATA	20	7.0280785E-4	45.000004	25
ATCCGTT	20	7.0280785E-4	45.000004	39
CTAACGG	20	7.0280785E-4	45.000004	2
TATTCGC	20	7.0280785E-4	45.000004	24
CGTACTA	20	7.0280785E-4	45.000004	10
CAATCTC	20	7.0280785E-4	45.000004	37
ACATACG	35	1.2094279E-7	45.000004	17
CCGTTTA	20	7.0280785E-4	45.000004	41
GCGTATG	20	7.0280785E-4	45.000004	1
CCGTTAA	20	7.0280785E-4	45.000004	30
GCGTAAG	40	6.7975634E-9	45.000004	1
CGCCCGT	20	7.0280785E-4	45.000004	2
ATCTGGC	35	1.2094279E-7	45.000004	18
ACGAAAC	20	7.0280785E-4	45.000004	6
CGGAACT	25	3.8864906E-5	45.0	27