##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550920_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415448 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.362692803912886 31.0 31.0 33.0 28.0 34.0 2 31.286047832701083 31.0 31.0 34.0 28.0 34.0 3 31.217365350176195 31.0 31.0 34.0 28.0 34.0 4 35.052278504168996 37.0 35.0 37.0 32.0 37.0 5 35.33149997111552 37.0 35.0 37.0 33.0 37.0 6 35.268481253971615 37.0 35.0 37.0 33.0 37.0 7 35.65455845256206 37.0 35.0 37.0 35.0 37.0 8 35.37112225838131 37.0 35.0 37.0 33.0 37.0 9 37.26100498738711 39.0 37.0 39.0 34.0 39.0 10 36.61968044135487 38.0 35.0 39.0 32.0 39.0 11 36.66649255743198 39.0 35.0 39.0 32.0 39.0 12 36.62663438023531 39.0 35.0 39.0 33.0 39.0 13 36.60834809651268 39.0 35.0 39.0 33.0 39.0 14 37.48166557547515 39.0 36.0 41.0 33.0 41.0 15 37.67507124838728 39.0 36.0 41.0 33.0 41.0 16 37.67005497679613 39.0 36.0 41.0 33.0 41.0 17 37.550345650959926 39.0 35.0 41.0 33.0 41.0 18 37.5442101057172 39.0 36.0 41.0 33.0 41.0 19 37.43319500876163 39.0 35.0 41.0 33.0 41.0 20 37.56562313454391 39.0 35.0 41.0 33.0 41.0 21 37.44515559107277 39.0 35.0 41.0 33.0 41.0 22 37.62557768962662 39.0 35.0 41.0 33.0 41.0 23 37.72427114825442 39.0 35.0 41.0 34.0 41.0 24 37.63405287785716 39.0 35.0 41.0 33.0 41.0 25 37.328175848722346 39.0 35.0 41.0 33.0 41.0 26 37.41817748550962 39.0 35.0 41.0 33.0 41.0 27 37.48776982919643 39.0 35.0 41.0 33.0 41.0 28 37.42088059155418 39.0 35.0 41.0 33.0 41.0 29 37.432716007779554 39.0 35.0 41.0 33.0 41.0 30 37.13656101365273 39.0 35.0 41.0 32.0 41.0 31 37.079273459012924 39.0 35.0 41.0 32.0 41.0 32 37.03979559415378 39.0 35.0 41.0 32.0 41.0 33 37.05387677880264 39.0 35.0 41.0 32.0 41.0 34 36.99194122970865 39.0 35.0 41.0 32.0 41.0 35 36.94420481022896 39.0 35.0 41.0 32.0 41.0 36 36.91028961506615 39.0 35.0 41.0 32.0 41.0 37 36.91145462247983 39.0 35.0 41.0 32.0 41.0 38 36.74116856983305 39.0 35.0 41.0 32.0 41.0 39 36.726644489803775 39.0 35.0 41.0 32.0 41.0 40 36.613667655157805 39.0 35.0 41.0 31.0 41.0 41 36.50388255569891 39.0 35.0 40.0 31.0 41.0 42 36.51561928327973 39.0 35.0 41.0 31.0 41.0 43 36.463906433536806 39.0 35.0 40.0 31.0 41.0 44 36.36375912268202 38.0 35.0 40.0 31.0 41.0 45 36.32315476305097 38.0 35.0 40.0 31.0 41.0 46 36.191619649149835 38.0 35.0 40.0 31.0 41.0 47 36.15721341780439 38.0 35.0 40.0 31.0 41.0 48 36.06510802796018 38.0 35.0 40.0 31.0 41.0 49 36.08376018177967 38.0 35.0 40.0 31.0 41.0 50 35.959169378598524 38.0 35.0 40.0 31.0 41.0 51 34.79459763917506 36.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 11.0 10 16.0 11 19.0 12 14.0 13 18.0 14 19.0 15 25.0 16 43.0 17 72.0 18 124.0 19 214.0 20 381.0 21 614.0 22 920.0 23 1362.0 24 1817.0 25 2406.0 26 3110.0 27 3909.0 28 4389.0 29 5378.0 30 6801.0 31 9080.0 32 12023.0 33 17934.0 34 37150.0 35 37788.0 36 32718.0 37 49628.0 38 87509.0 39 99933.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83267219964954 21.17569467177601 24.626668078796865 12.36496504977759 2 31.506951531840325 25.417140051221814 25.17306618397489 17.90284223296297 3 32.71263792339836 24.845227320868073 26.001810094163407 16.44032466157016 4 29.93154377924554 25.03490208160829 26.166210933739 18.867343205407174 5 26.594423369471027 29.645106006046483 24.13250274402573 19.627967880456758 6 26.61512391442491 34.562448248637615 23.318201074502706 15.504226762434769 7 78.88544414704126 6.050817430821668 10.217644566829062 4.846093855308005 8 79.06236159519362 6.3021124184013395 8.926748955344593 5.708777031060445 9 73.32734782692418 7.026872195798271 10.973455161656814 8.672324815620728 10 45.83245075195933 22.09686892222372 17.753605746086155 14.317074579730797 11 35.59242071209875 22.63508309102463 22.467071691282666 19.30542450559396 12 33.076582388168916 19.824382353507538 27.095087712541645 20.0039475457819 13 26.89073000712484 22.017195894552387 29.92793321907916 21.16414087924361 14 20.552993395081938 26.57974042479444 29.263590148466236 23.603676031657393 15 21.464058077063797 23.786851784098133 34.84936742985885 19.899722708979223 16 25.784695076158748 23.335531763301304 29.21400512218136 21.66576803835859 17 24.31038300822245 24.086768981918315 28.25504034199226 23.347807667866977 18 24.39125955594924 25.437840596175693 29.162735167818838 21.00816468005623 19 25.86581232789663 26.529433286476284 25.86581232789663 21.73894205773045 20 26.339758525736073 27.544241397238643 27.131674722227572 18.98432535479771 21 26.324353469026207 27.592863607479156 27.032263965646724 19.050518957847913 22 23.2895572971828 24.520758313916545 27.802516801139976 24.387167587760683 23 23.334568947256937 24.31303075234446 29.15984671968574 23.192553580712868 24 26.09592536250024 22.39437907993299 28.369856155282974 23.139839402283798 25 23.520151739808593 25.056806146617628 25.66410236660184 25.758939746971944 26 20.29832855134698 25.082802179815523 28.310402264543338 26.30846700429416 27 22.84786543682964 25.57191273035374 27.511024243708 24.069197589108626 28 19.820531089330075 26.920577304500203 28.320030424987003 24.938861181182723 29 23.000231075850646 25.428693843754214 27.35023396429878 24.22084111609636 30 26.021547823072922 24.31808553657738 28.416793437445843 21.243573202903853 31 26.621141514702202 25.015645760720957 26.155138549228784 22.20807417534806 32 27.392838574262 27.55362885367122 23.95390036779573 21.099632204271053 33 27.188962276867386 23.811162889218384 25.527382488301786 23.472492345612448 34 22.62377000250332 23.879522828368412 29.693968920298087 23.80273824883018 35 21.143440334289732 27.099901792763475 27.467216113689318 24.28944175925748 36 24.188105370587895 27.234696038974793 25.492239702682408 23.084958887754905 37 24.532552810460036 28.499114209239185 26.379474687566194 20.588858292734592 38 23.393060021952206 29.468910669927407 24.110117270994206 23.027912037126185 39 23.30279601779284 26.210500471779863 27.136729506460494 23.349974003966803 40 23.049816102135527 23.464067705224238 29.545935953476725 23.940180239163507 41 21.035364233309583 25.152847047043192 26.22181356030117 27.589975159346054 42 21.723537001020585 25.04140108990776 25.49079547861586 27.7442664304558 43 23.153800234927115 22.882286110415745 26.423764225607055 27.540149429050086 44 21.70211434403343 23.09891972039822 28.24011669330458 26.95884924226377 45 22.827646299897943 22.92826057653425 26.852698773372357 27.39139435019545 46 24.095675030328707 25.110723845102157 27.40415166278331 23.38944946178583 47 20.818008511293833 23.744006470123818 30.45844485952514 24.97954015905721 48 21.485721438062043 22.587905104850666 29.794342492923303 26.132030964163988 49 23.2837804009166 21.31458088617589 31.654021682617316 23.747617030290193 50 21.73003600932006 21.807783404902658 30.066097321445767 26.396083264331516 51 19.587770310604455 22.389324295700064 28.69697290635651 29.325932487338967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 475.5 2 691.0 3 1346.5 4 2002.0 5 1402.5 6 803.0 7 755.5 8 708.0 9 717.5 10 727.0 11 767.0 12 807.0 13 765.0 14 723.0 15 777.0 16 831.0 17 812.0 18 793.0 19 762.0 20 731.0 21 883.5 22 1036.0 23 991.5 24 947.0 25 1308.5 26 1976.0 27 2282.0 28 2699.5 29 3117.0 30 3729.0 31 4341.0 32 5239.5 33 6138.0 34 6709.0 35 7280.0 36 7653.0 37 8026.0 38 9794.5 39 11563.0 40 13517.5 41 15472.0 42 17393.0 43 19314.0 44 22161.0 45 25008.0 46 32584.0 47 40160.0 48 40958.5 49 41757.0 50 41617.5 51 41478.0 52 36759.5 53 32041.0 54 30138.5 55 28236.0 56 26084.0 57 23932.0 58 23293.5 59 22655.0 60 21731.5 61 20808.0 62 18869.5 63 16931.0 64 14339.5 65 11748.0 66 9408.0 67 7068.0 68 5970.0 69 4872.0 70 4109.5 71 3347.0 72 2724.5 73 2102.0 74 1720.0 75 1114.5 76 891.0 77 638.5 78 386.0 79 308.0 80 230.0 81 158.5 82 87.0 83 70.5 84 54.0 85 50.5 86 47.0 87 26.0 88 5.0 89 4.0 90 3.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 415448.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.62774438504609 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8202472763149 21.99692175249775 2 11.37755106285999 6.969374513621705 3 4.0402765874645 3.71233675697247 4 1.9853706300079104 2.4322969666184076 5 1.151407412543921 1.7632505957221263 6 0.8277908045168927 1.5212019099021028 7 0.6715981865811993 1.4398676311648624 8 0.48888609173640357 1.197878260088541 9 0.4314039457582581 1.1891636799645797 >10 6.9275777353569685 48.42966610523733 >50 0.23459291649861957 4.425316853188433 >100 0.036999553944413156 2.0038579256210673 >500 0.0015744491040175813 0.34671491962977913 >1k 0.004723347312052743 2.572152129770851 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 2167 0.5216055920355857 TruSeq Adapter, Index 20 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC 2157 0.5191985519246693 No Hit GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC 1954 0.4703356376730662 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG 1763 0.42436117155456277 No Hit CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 1558 0.3750168492807764 TruSeq Adapter, Index 20 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT 1069 0.25731258785696404 TruSeq Adapter, Index 27 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.20435770541680306 No Hit TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 589 0.14177466253297644 TruSeq Adapter, Index 20 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT 435 0.10470624482486378 No Hit ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 434 0.10446554081377211 TruSeq Adapter, Index 27 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.8140802218328164E-4 0.0 0.0 0.2657372282451715 0.0 2 4.8140802218328164E-4 0.0 0.0 1.4815331882690492 0.0 3 4.8140802218328164E-4 0.0 0.0 1.9184109684003774 0.0 4 4.8140802218328164E-4 0.0 0.0 2.5006739712310564 0.0 5 4.8140802218328164E-4 0.0 0.0 4.391885386378078 0.0 6 4.8140802218328164E-4 0.0 0.0 5.176580462536828 0.0 7 4.8140802218328164E-4 0.0 0.0 6.133138202615008 0.0 8 4.8140802218328164E-4 0.0 0.0 7.504669657815178 0.0 9 4.8140802218328164E-4 0.0 0.0 7.961766574878204 0.0 10 4.8140802218328164E-4 0.0 0.0 9.389863472684908 0.0 11 4.8140802218328164E-4 0.0 0.0 11.041333692784656 0.0 12 4.8140802218328164E-4 0.0 0.0 12.788122701276695 0.0 13 4.8140802218328164E-4 0.0 0.0 13.31574589358957 0.0 14 4.8140802218328164E-4 0.0 0.0 13.546821744237546 0.0 15 4.8140802218328164E-4 0.0 0.0 14.002474437234023 0.0 16 4.8140802218328164E-4 0.0 0.0 14.800648938013904 0.0 17 4.8140802218328164E-4 0.0 0.0 15.701363347518823 0.0 18 7.221120332749225E-4 0.0 0.0 16.62253759796653 0.0 19 7.221120332749225E-4 0.0 0.0 17.361739616028963 0.0 20 7.221120332749225E-4 0.0 0.0 18.008029885810018 0.0 21 7.221120332749225E-4 0.0 0.0 18.777560609269994 0.0 22 7.221120332749225E-4 0.0 0.0 19.54877626080761 0.0 23 7.221120332749225E-4 0.0 0.0 20.250669157150835 0.0 24 7.221120332749225E-4 0.0 0.0 20.8793880341222 0.0 25 7.221120332749225E-4 0.0 0.0 21.39016194565866 0.0 26 7.221120332749225E-4 0.0 0.0 21.86218251140937 0.0 27 7.221120332749225E-4 0.0 0.0 22.34190560551501 0.0 28 7.221120332749225E-4 0.0 0.0 22.811519131154803 0.0 29 7.221120332749225E-4 0.0 0.0 23.298704049604282 0.0 30 7.221120332749225E-4 0.0 0.0 23.84101018659375 0.0 31 7.221120332749225E-4 0.0 0.0 24.381631395505575 0.0 32 7.221120332749225E-4 0.0 0.0 24.90468121160771 0.0 33 7.221120332749225E-4 0.0 0.0 25.390662610001733 0.0 34 7.221120332749225E-4 0.0 0.0 25.93128381891356 0.0 35 7.221120332749225E-4 0.0 0.0 26.4627582754039 0.0 36 7.221120332749225E-4 0.0 0.0 26.989900059694595 0.0 37 7.221120332749225E-4 0.0 0.0 27.51704184398529 0.0 38 7.221120332749225E-4 0.0 0.0 28.12313454391404 0.0 39 7.221120332749225E-4 0.0 0.0 28.79469873485972 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTACG 20 7.0280785E-4 45.000004 12 CGTATGG 40 6.7975634E-9 45.000004 2 TACCAAT 20 7.0280785E-4 45.000004 26 CGCATCG 20 7.0280785E-4 45.000004 21 TTGTCGG 20 7.0280785E-4 45.000004 2 GCGCATA 20 7.0280785E-4 45.000004 25 ATCCGTT 20 7.0280785E-4 45.000004 39 CTAACGG 20 7.0280785E-4 45.000004 2 TATTCGC 20 7.0280785E-4 45.000004 24 CGTACTA 20 7.0280785E-4 45.000004 10 CAATCTC 20 7.0280785E-4 45.000004 37 ACATACG 35 1.2094279E-7 45.000004 17 CCGTTTA 20 7.0280785E-4 45.000004 41 GCGTATG 20 7.0280785E-4 45.000004 1 CCGTTAA 20 7.0280785E-4 45.000004 30 GCGTAAG 40 6.7975634E-9 45.000004 1 CGCCCGT 20 7.0280785E-4 45.000004 2 ATCTGGC 35 1.2094279E-7 45.000004 18 ACGAAAC 20 7.0280785E-4 45.000004 6 CGGAACT 25 3.8864906E-5 45.0 27 >>END_MODULE