FastQCFastQC Report
Sat 18 Jun 2016
SRR3550918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567934
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC42790.7534326171703051No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG38210.6727894438438269No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC34000.5986611120306233No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC28780.506749023654157No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC25440.4479393732370311No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT12500.22009599707008207Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.17924618001387485No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8880.1563561963185863No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT8830.155475812330306No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT7850.13822028616001156No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC7370.1297685998725204No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6850.12061260639440498No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC6160.10846330735613645No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT5740.1010680818545817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT207.030268E-445.00000443
CGAAAGG302.1635533E-645.0000042
CGCATAC207.030268E-445.00000432
CGGTGTA207.030268E-445.00000430
CGAATAT1450.045.00000414
ACTCGCA302.1635533E-645.00000412
CGTAAGG406.8048394E-945.0000042
CGTAACG207.030268E-445.0000042
TTACGCG207.030268E-445.0000041
TTGCCGC207.030268E-445.00000433
CGATGCG253.8883038E-545.00000410
CGACTCG207.030268E-445.00000430
GCGCTAA207.030268E-445.0000041
CGATCGA207.030268E-445.00000441
TAAGACG207.030268E-445.0000041
GCGTAAG406.8048394E-945.0000041
ATGATCG453.8380676E-1045.027
TACGAAT1600.042.18750412
GCTACGA1600.042.18750410
GCGAACG603.6379788E-1241.2500041