##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567934
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48966429197759	31.0	31.0	33.0	30.0	34.0
2	31.37950888659598	31.0	31.0	34.0	28.0	34.0
3	31.333213014188267	31.0	31.0	34.0	28.0	34.0
4	35.13604045540503	37.0	35.0	37.0	32.0	37.0
5	35.42818179577204	37.0	35.0	37.0	33.0	37.0
6	35.38138234372304	37.0	35.0	37.0	33.0	37.0
7	35.75945092211419	37.0	35.0	37.0	35.0	37.0
8	35.43672152045836	37.0	35.0	37.0	33.0	37.0
9	37.39528536766596	39.0	37.0	39.0	34.0	39.0
10	36.76694122908648	39.0	35.0	39.0	32.0	39.0
11	36.89147682653266	39.0	37.0	39.0	33.0	39.0
12	37.05099360136917	39.0	37.0	39.0	33.0	39.0
13	37.10750192804093	39.0	37.0	39.0	33.0	39.0
14	38.1309870513123	40.0	37.0	41.0	33.0	41.0
15	38.26296893653136	40.0	38.0	41.0	34.0	41.0
16	38.28310860064726	40.0	38.0	41.0	34.0	41.0
17	38.186766420041764	40.0	37.0	41.0	34.0	41.0
18	38.17314159743914	40.0	37.0	41.0	34.0	41.0
19	38.03318519405424	40.0	37.0	41.0	34.0	41.0
20	38.159104755129995	40.0	37.0	41.0	34.0	41.0
21	38.05668440346942	40.0	37.0	41.0	34.0	41.0
22	38.21746717048108	40.0	37.0	41.0	34.0	41.0
23	38.301954100300385	40.0	37.0	41.0	34.0	41.0
24	38.22315445104537	40.0	37.0	41.0	34.0	41.0
25	37.92127958530393	40.0	37.0	41.0	33.0	41.0
26	38.04674838977768	40.0	37.0	41.0	34.0	41.0
27	38.13082329989048	40.0	37.0	41.0	34.0	41.0
28	38.049195857265104	40.0	37.0	41.0	34.0	41.0
29	38.07889825226171	40.0	37.0	41.0	34.0	41.0
30	37.83511992590688	40.0	37.0	41.0	33.0	41.0
31	37.79616293442548	40.0	37.0	41.0	33.0	41.0
32	37.775922906534916	40.0	37.0	41.0	33.0	41.0
33	37.75373723003025	40.0	37.0	41.0	33.0	41.0
34	37.73632499551004	40.0	37.0	41.0	33.0	41.0
35	37.72223004785767	40.0	37.0	41.0	33.0	41.0
36	37.51729426306578	40.0	36.0	41.0	33.0	41.0
37	37.547234361739214	40.0	36.0	41.0	33.0	41.0
38	37.3877404768864	40.0	36.0	41.0	33.0	41.0
39	37.39663235516803	40.0	36.0	41.0	33.0	41.0
40	37.351438723513645	40.0	36.0	41.0	33.0	41.0
41	37.17645888430698	39.0	35.0	41.0	32.0	41.0
42	37.18889518852543	39.0	35.0	41.0	32.0	41.0
43	37.09080808685516	39.0	35.0	41.0	32.0	41.0
44	37.078519687146745	39.0	35.0	41.0	32.0	41.0
45	37.08559621364454	39.0	35.0	41.0	32.0	41.0
46	36.98901280782626	39.0	35.0	41.0	32.0	41.0
47	36.9535157254188	39.0	35.0	41.0	32.0	41.0
48	36.84658252543429	39.0	35.0	41.0	32.0	41.0
49	36.85408163624646	39.0	35.0	41.0	32.0	41.0
50	36.71385583536115	39.0	35.0	41.0	31.0	41.0
51	35.57242038687594	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	17.0
10	27.0
11	19.0
12	10.0
13	18.0
14	15.0
15	26.0
16	32.0
17	57.0
18	130.0
19	250.0
20	450.0
21	782.0
22	1059.0
23	1435.0
24	1893.0
25	2629.0
26	3310.0
27	4060.0
28	4827.0
29	5606.0
30	7151.0
31	9698.0
32	13051.0
33	18877.0
34	33927.0
35	39849.0
36	43507.0
37	70041.0
38	133593.0
39	171549.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60002042490853	20.68691080301584	25.029316786809737	11.683751985265893
2	29.902066085143698	26.744656949575123	25.696119619533253	17.65715734574792
3	31.63466177407938	25.409290516151522	27.002785534938916	15.953262174830174
4	29.272943687118573	24.806931791370125	27.598101187814077	18.322023333697228
5	26.550092088165172	30.151038677029373	23.915278888039808	19.383590346765644
6	25.532720351308424	36.047850630531016	23.327182383868546	15.092246634292014
7	79.47578415801836	5.637450830554255	10.410540661414883	4.476224350012502
8	79.9152718449679	7.294157419700177	7.79122221948325	4.999348515848673
9	74.34913211746435	7.1937936450362185	9.841284374592822	8.615789862906606
10	38.701151894410266	31.713368102631645	16.54822567411002	13.03725432884807
11	27.679976898724146	24.62398799860547	28.284272468279763	19.411762634390616
12	26.076973732863323	22.474618529617878	30.803931442738065	20.64447629478073
13	23.69940873411347	24.814150940074022	32.5351185172925	18.951321808520007
14	19.438174154039025	29.339148563037256	28.862860825377595	22.359816457546124
15	18.201234650505164	27.823303412016188	34.14639729264316	19.82906464483549
16	20.12945166163674	27.38945018259164	30.13061376850127	22.350484387270352
17	20.719485010582215	27.80956942179901	28.29959114967584	23.17135441794293
18	21.441399880972085	26.709617666841567	30.013522698059987	21.83545975412636
19	22.065592128662836	27.260738043505057	28.383227628562473	22.290442199269634
20	23.995922061366283	27.066349258892757	29.902946469131976	19.03478221060898
21	23.618413407191678	28.41016737860385	29.015519408945405	18.95589980525906
22	22.21983540340955	24.20739029535122	28.648751439427823	24.924022861811405
23	20.585666644363606	27.631731856166386	29.232622100455334	22.549979399014674
24	22.317558026108667	25.29765782643758	29.46345878218244	22.921325365271315
25	20.240380044160062	29.814379839910977	27.146112048230957	22.799128067698007
26	20.177344550599187	28.170879010589257	28.74753052291287	22.904245915898677
27	22.73450788295823	28.27423609081337	27.896199206245797	21.095056819982606
28	18.854127416213856	27.69071758338117	31.594340187416144	21.86081481298883
29	21.03166917282642	26.05390767237038	30.836681727102093	22.077741427701106
30	24.22041997837777	26.033834917437588	29.047741462916466	20.698003641268176
31	23.61084210489247	26.466807762873856	27.570457130582078	22.3518930016516
32	23.565414291097206	28.981184433402476	27.610778717245317	19.84262255825501
33	24.155799793637993	26.151806371867153	27.160022115245784	22.532371719249067
34	23.564005676715958	24.487880634017333	30.166709512020763	21.781404177245946
35	23.79378589765712	25.08178767251124	27.98458975866914	23.139836671162495
36	24.274827708853493	25.357700014438294	29.695880155088446	20.671592121619767
37	23.743075779932173	27.049798039913085	30.121809928618465	19.08531625153627
38	22.828356816108915	27.05367172946152	27.814675648931036	22.30329580549853
39	23.91422242725387	25.00448995834023	29.53952395876986	21.541763655636043
40	26.270482133487345	23.72582025376188	29.094577891092975	20.9091197216578
41	22.357527459176595	25.10379727221825	28.881348889131488	23.65732637947367
42	23.25411051284128	24.746361372976438	30.417442871883	21.582085242299282
43	24.11389351579585	24.375543637112763	29.984998256839702	21.525564590251683
44	22.526737261724072	25.147464318036956	29.781805632344604	22.543992787894368
45	22.383410748432038	24.398609697605707	28.395905157993713	24.822074395968546
46	25.03459909073942	25.474967161677238	28.10872390101667	21.38170984656668
47	21.888106716625526	24.65532966858825	32.106371514999985	21.35019209978624
48	22.436057710931202	24.16530794071142	29.99362601992485	23.405008328432526
49	22.033017921096466	23.083492095912554	32.6293196040385	22.254170378952484
50	22.00678247824571	23.506780717477735	30.473435293537634	24.013001510738924
51	21.521338747107936	23.036127437343072	28.13918518701117	27.303348628537822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	537.5
2	831.0
3	1468.5
4	2106.0
5	1508.0
6	910.0
7	915.0
8	920.0
9	890.0
10	860.0
11	887.0
12	914.0
13	889.0
14	864.0
15	917.0
16	970.0
17	998.0
18	1026.0
19	1174.0
20	1322.0
21	1561.5
22	1801.0
23	2077.0
24	2353.0
25	3250.0
26	4742.5
27	5338.0
28	6933.5
29	8529.0
30	9440.0
31	10351.0
32	12244.0
33	14137.0
34	16099.0
35	18061.0
36	18590.5
37	19120.0
38	21105.5
39	23091.0
40	24973.0
41	26855.0
42	30691.5
43	34528.0
44	37373.5
45	40219.0
46	51137.0
47	62055.0
48	59996.0
49	57937.0
50	56803.0
51	55669.0
52	47034.5
53	38400.0
54	33619.0
55	28838.0
56	26466.5
57	24095.0
58	22613.5
59	21132.0
60	19709.0
61	18286.0
62	16278.5
63	14271.0
64	12026.5
65	9782.0
66	8223.5
67	6665.0
68	5507.0
69	4349.0
70	3597.5
71	2846.0
72	2344.5
73	1843.0
74	1567.0
75	863.0
76	435.0
77	365.5
78	296.0
79	202.5
80	109.0
81	105.0
82	101.0
83	62.0
84	23.0
85	15.5
86	8.0
87	5.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.020391930472698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65756578217724	20.07873078000236
2	11.056526445213192	6.196164087690194
3	3.8766569766552483	3.2587634359764426
4	1.900233809978399	2.129811844605205
5	1.124314608050673	1.5751867985367825
6	0.8037563443840313	1.3512940671746727
7	0.5959289900723969	1.1688714705191534
8	0.49680927438829914	1.1136632466443115
9	0.45444248854433394	1.1460290974984428
>10	7.761672242354968	51.70019909889726
>50	0.21716938546251022	3.7246908955733113
>100	0.04545405742238586	2.151037295315894
>500	0.005050450824709541	1.0109521498955867
>1k	0.004419144471620848	3.394605731670395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	4279	0.7534326171703051	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	3821	0.6727894438438269	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	3400	0.5986611120306233	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2878	0.506749023654157	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2544	0.4479393732370311	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	1250	0.22009599707008207	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1018	0.17924618001387485	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	888	0.1563561963185863	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	883	0.155475812330306	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	785	0.13822028616001156	No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	737	0.1297685998725204	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.12061260639440498	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	616	0.10846330735613645	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	574	0.1010680818545817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23365391048959913	0.0
2	0.0	0.0	0.0	1.4758757179531425	0.0
3	0.0	0.0	0.0	1.8588427528550853	0.0
4	0.0	0.0	0.0	2.4103152831138828	0.0
5	0.0	0.0	0.0	4.282715949388486	0.0
6	0.0	0.0	0.0	4.993714058323678	0.0
7	0.0	0.0	0.0	5.837121919096233	0.0
8	0.0	0.0	0.0	7.058214510841048	0.0
9	0.0	0.0	0.0	7.427975785918786	0.0
10	0.0	0.0	0.0	8.978684142875757	0.0
11	0.0	0.0	0.0	10.318628573038417	0.0
12	0.0	0.0	0.0	12.141551659171665	0.0
13	0.0	0.0	0.0	12.606570481781334	0.0
14	0.0	0.0	0.0	12.804304725549095	0.0
15	0.0	0.0	0.0	13.219317737624442	0.0
16	0.0	0.0	0.0	13.921335929879175	0.0
17	0.0	0.0	0.0	14.786577313561082	0.0
18	1.7607679765606568E-4	0.0	0.0	15.68809051756014	0.0
19	1.7607679765606568E-4	0.0	0.0	16.41528769187969	0.0
20	1.7607679765606568E-4	0.0	0.0	17.005321040825166	0.0
21	1.7607679765606568E-4	0.0	0.0	17.64553627710262	0.0
22	1.7607679765606568E-4	0.0	0.0	18.400905739047143	0.0
23	1.7607679765606568E-4	0.0	0.0	19.084611944345646	0.0
24	1.7607679765606568E-4	0.0	0.0	19.653868231167706	0.0
25	1.7607679765606568E-4	0.0	0.0	20.129099508041428	0.0
26	1.7607679765606568E-4	0.0	0.0	20.576862804480804	0.0
27	1.7607679765606568E-4	0.0	0.0	21.046283547031873	0.0
28	1.7607679765606568E-4	0.0	0.0	21.50302676015171	0.0
29	1.7607679765606568E-4	0.0	0.0	21.999035099148845	0.0
30	1.7607679765606568E-4	0.0	0.0	22.52251141858033	0.0
31	1.7607679765606568E-4	0.0	0.0	23.016230759207936	0.0
32	1.7607679765606568E-4	0.0	0.0	23.491109882486345	0.0
33	1.7607679765606568E-4	0.0	0.0	23.947148788415554	0.0
34	1.7607679765606568E-4	0.0	0.0	24.39772931361743	0.0
35	1.7607679765606568E-4	0.0	0.0	24.906239105248144	0.0
36	3.5215359531213137E-4	0.0	0.0	25.371081851060158	0.0
37	3.5215359531213137E-4	0.0	0.0	25.853708353435433	0.0
38	3.5215359531213137E-4	0.0	0.0	26.27770128219124	0.0
39	5.28230392968197E-4	0.0	0.0	26.734796648906386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGT	20	7.030268E-4	45.000004	43
CGAAAGG	30	2.1635533E-6	45.000004	2
CGCATAC	20	7.030268E-4	45.000004	32
CGGTGTA	20	7.030268E-4	45.000004	30
CGAATAT	145	0.0	45.000004	14
ACTCGCA	30	2.1635533E-6	45.000004	12
CGTAAGG	40	6.8048394E-9	45.000004	2
CGTAACG	20	7.030268E-4	45.000004	2
TTACGCG	20	7.030268E-4	45.000004	1
TTGCCGC	20	7.030268E-4	45.000004	33
CGATGCG	25	3.8883038E-5	45.000004	10
CGACTCG	20	7.030268E-4	45.000004	30
GCGCTAA	20	7.030268E-4	45.000004	1
CGATCGA	20	7.030268E-4	45.000004	41
TAAGACG	20	7.030268E-4	45.000004	1
GCGTAAG	40	6.8048394E-9	45.000004	1
ATGATCG	45	3.8380676E-10	45.0	27
TACGAAT	160	0.0	42.187504	12
GCTACGA	160	0.0	42.187504	10
GCGAACG	60	3.6379788E-12	41.250004	1
>>END_MODULE