FastQCFastQC Report
Sat 18 Jun 2016
SRR3550917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102983
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.518865657947584No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC56710.5141511700542982No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC52940.47997113282797654No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG44570.4040860103918193No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC34490.31269747584504926No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC22970.20825343636302643No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT17570.15929529285582825No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG16910.15331151976050403No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT15600.1414346367985726No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT13770.12484326594335543No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC13300.12058209419365486No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT11970.10852388477428936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG551.8189894E-1245.01
CGTTTTT39100.042.1227651
TCTAGCG650.041.538461
GCTACGA1250.041.410
TCGATAG501.0822987E-940.51
CTACGAA1300.039.8076911
CGCAAGG2100.038.571432
CGACGGT700.038.57142628
TACGGCT6250.038.167
TCAAGCG2450.037.6530617
CTCGATC552.748493E-936.8181816
CGAATAT1350.036.66666414
CACAACG2800.036.16071312
TACGAAT1500.036.00000412
AGACACG2500.036.00000424
GGTCGTA250.002107071736.08
AATGGGC3250.036.04
CGCTAAT250.002107071736.021
AACTACG250.002107071736.013
AAGGGCG3400.035.7352945
Produced by FastQC (version 0.11.5)