FastQCFastQC Report
Sat 18 Jun 2016
SRR3550916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642247
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC36500.5683171739221826No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC30620.4767636127533488No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG27680.4309868321689319No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC27600.4297412054863627Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC17370.27045669345283047Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT13290.20692973264180292Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.18264001233170415No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8180.12736532829269737No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT7100.11054936807801359No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC7090.11039366474269247Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT6770.10541115801241578No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6630.10323131131791974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG302.163999E-645.0000041
CCGATGT302.163999E-645.00000411
TTATCGG253.8888757E-545.0000042
CTTGCGG502.1827873E-1145.0000042
TATAGCG302.163999E-645.0000041
CGTAACA302.163999E-645.00000418
ACGTAAC302.163999E-645.00000417
CTTTAGC302.163999E-645.00000419
GTACGAG253.8888757E-545.0000041
GACGTAG207.03096E-445.01
ATCACGG207.03096E-445.02
TATGATC207.03096E-445.033
TAACACG207.03096E-445.01
CCGAACA207.03096E-445.014
ACGGTCT406.8066583E-945.030
GCTACGA1450.043.4482810
TACGGGA1650.040.9090924
ACGGGTC501.0804797E-940.5000045
TACGGGT451.925946E-840.04
CGGTCTA451.925946E-840.031