##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642247
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.396275887625787	31.0	31.0	33.0	28.0	34.0
2	31.30272776673149	31.0	31.0	34.0	28.0	34.0
3	31.226605963126335	31.0	31.0	34.0	28.0	34.0
4	35.091069323795985	37.0	35.0	37.0	32.0	37.0
5	35.371587566777265	37.0	35.0	37.0	33.0	37.0
6	35.304412476819664	37.0	35.0	37.0	33.0	37.0
7	35.6854154242838	37.0	35.0	37.0	35.0	37.0
8	35.32832228099158	37.0	35.0	37.0	33.0	37.0
9	37.27071516098946	39.0	37.0	39.0	34.0	39.0
10	36.63894576385721	38.0	35.0	39.0	32.0	39.0
11	36.78724696261719	39.0	37.0	39.0	33.0	39.0
12	36.90084033090073	39.0	37.0	39.0	33.0	39.0
13	36.92336904648835	39.0	37.0	39.0	33.0	39.0
14	37.8774801595025	40.0	37.0	41.0	33.0	41.0
15	38.025279993522744	40.0	37.0	41.0	33.0	41.0
16	38.052398843435626	40.0	37.0	41.0	33.0	41.0
17	37.938743193817956	40.0	37.0	41.0	33.0	41.0
18	37.91618800866333	39.0	37.0	41.0	33.0	41.0
19	37.79684295917303	39.0	37.0	41.0	33.0	41.0
20	37.93746954053503	40.0	36.0	41.0	34.0	41.0
21	37.79345641162979	39.0	36.0	41.0	33.0	41.0
22	37.96291613662656	40.0	36.0	41.0	34.0	41.0
23	38.057529268334456	40.0	37.0	41.0	34.0	41.0
24	37.94883899808018	40.0	36.0	41.0	34.0	41.0
25	37.62106946392899	39.0	36.0	41.0	33.0	41.0
26	37.71628049644451	39.0	36.0	41.0	33.0	41.0
27	37.82414086792153	40.0	36.0	41.0	33.0	41.0
28	37.74374967886187	40.0	36.0	41.0	33.0	41.0
29	37.771653273584775	40.0	36.0	41.0	33.0	41.0
30	37.504746616177265	40.0	36.0	41.0	33.0	41.0
31	37.47554756970449	40.0	36.0	41.0	33.0	41.0
32	37.45811191021523	40.0	36.0	41.0	33.0	41.0
33	37.472941095871214	40.0	36.0	41.0	33.0	41.0
34	37.405239728640225	40.0	36.0	41.0	33.0	41.0
35	37.4040392559249	40.0	36.0	41.0	33.0	41.0
36	37.31752114062035	40.0	35.0	41.0	33.0	41.0
37	37.31403494294251	40.0	35.0	41.0	33.0	41.0
38	37.17423203222436	40.0	35.0	41.0	32.0	41.0
39	37.13195234855126	39.0	35.0	41.0	32.0	41.0
40	37.02594173269786	39.0	35.0	41.0	32.0	41.0
41	36.889393021687916	39.0	35.0	41.0	31.0	41.0
42	36.913900726667464	39.0	35.0	41.0	32.0	41.0
43	36.86971056306997	39.0	35.0	41.0	32.0	41.0
44	36.822252186464084	39.0	35.0	41.0	32.0	41.0
45	36.781635414412214	39.0	35.0	41.0	32.0	41.0
46	36.66425534101366	39.0	35.0	41.0	31.0	41.0
47	36.62833458155507	39.0	35.0	41.0	31.0	41.0
48	36.542040679053386	39.0	35.0	40.0	31.0	41.0
49	36.525230946972115	39.0	35.0	40.0	31.0	41.0
50	36.41261383860104	39.0	35.0	40.0	31.0	41.0
51	35.23326695181137	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	23.0
10	19.0
11	25.0
12	18.0
13	20.0
14	27.0
15	27.0
16	59.0
17	81.0
18	173.0
19	315.0
20	546.0
21	851.0
22	1337.0
23	1847.0
24	2615.0
25	3601.0
26	4465.0
27	5325.0
28	6239.0
29	7393.0
30	9222.0
31	11973.0
32	16512.0
33	23806.0
34	44121.0
35	48792.0
36	51370.0
37	79277.0
38	144176.0
39	177949.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42145623101392	20.95097369080743	24.004471799790423	11.623098278388222
2	31.77531385899817	25.90218405068455	24.533707436546997	17.788794653770278
3	31.59096110997794	25.496576862172965	26.594285376187045	16.318176651662057
4	29.79212047701274	24.86691257413425	26.220908778086937	19.12005817076608
5	26.495880868264077	30.359659134258315	23.520701536947623	19.623758460529984
6	26.207673994584642	35.86019086114844	23.228602079885153	14.703533064381771
7	78.74275784861588	6.222216686103633	10.047536228273547	4.987489237006946
8	78.62239917041262	7.3516886805232255	8.494239755109795	5.531672393954351
9	72.78741667925269	8.011870822284886	10.724378626914568	8.476333871547864
10	41.85056528095888	25.86746220690793	17.696306872589517	14.585665639543667
11	31.570252566380226	25.088011310290277	23.401432782091625	19.940303341237872
12	29.527113400296145	22.19286349332889	27.85470387561172	20.425319230763243
13	25.749594782069828	24.345773510814375	29.48927748981311	20.41535421730269
14	20.227108884899422	27.976463883832857	29.142370458717597	22.654056772550124
15	19.864787223607117	25.94780512793364	34.33336395498928	19.854043693469958
16	22.01053488766783	25.563684999696378	30.571882780301035	21.853897332334757
17	21.856855695705857	25.483497782005987	29.063584571045098	23.59606195124306
18	23.027433370650233	25.98922221512907	28.938243386111573	22.045101028109123
19	23.97037276935509	27.940963523379637	26.38470868684478	21.703955020420494
20	26.401991757065428	25.99591745854788	28.457587189975197	19.144503594411496
21	24.038415126890435	27.539093214915756	27.966031760366338	20.45645989782747
22	22.580720501613865	24.851186537266816	27.809549908368584	24.758543052750735
23	23.00843756374105	26.468009971241596	28.09355279199436	22.429999673022998
24	23.9468615657216	24.381118167932275	28.195538476629707	23.476481789716416
25	22.266511170935793	27.729051284007554	25.83569872650242	24.168738818554232
26	21.005002748163868	27.548591118370346	27.647151329628635	23.79925480383715
27	23.066047797809876	26.770385848435257	27.538003291568508	22.625563062186355
28	19.0716344334812	27.87899359592182	28.452293276574274	24.597078694022706
29	21.510727181286953	25.971472034902458	27.342439902405147	25.17536088140544
30	21.228592737685034	26.320091802686505	28.308111988066898	24.143203471561563
31	23.0934515848264	27.34913514582396	24.375824254531356	25.181589014818286
32	24.24596767287352	28.679464442807827	24.693926168592455	22.380641715726192
33	22.201427176771553	27.97802091718607	24.83546050039938	24.985091405643
34	20.807415215641335	27.84255901545667	26.345627149679174	25.004398619222822
35	21.6370025862324	26.905692046829333	25.776064349074424	25.681241017863844
36	20.657940013733032	29.67191750214481	24.692213431903927	24.97792905221823
37	21.717812617264073	28.994763696833147	27.524301398060246	21.763122287842528
38	21.148716926665287	28.77600051070694	24.61669731427317	25.458585248354602
39	22.7945011810098	27.451432237129954	25.91059203079189	23.843474551068358
40	22.11937151905731	25.13861489426965	28.2705874842545	24.47142610241854
41	20.159222230699402	28.284756487768725	26.61561673312604	24.94040454840583
42	22.266666874271113	26.14694969380939	27.16057840675005	24.425805025169446
43	22.26106155419955	26.022854135558436	26.767894595070118	24.94818971517189
44	20.680984107360565	25.71082465157486	26.630875659987513	26.97731558107706
45	21.732760137454903	24.344216477461163	25.90950210744464	28.013521277639285
46	22.565772981423034	26.143212813761686	26.28832832228099	25.002685882534294
47	19.891568197282353	25.748349155387256	30.10835395104999	24.251728696280402
48	20.683163954055058	25.32904007336741	28.224032186993476	25.763763785584047
49	21.15183099337171	24.651263454714464	29.430265925726395	24.766639626187434
50	20.076699462979196	24.847138250548465	28.60207988515322	26.474082401319123
51	20.12745875029389	24.466599299023585	26.670424307159085	28.73551764352344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	702.5
2	1173.0
3	1934.0
4	2695.0
5	1978.0
6	1261.0
7	1221.0
8	1181.0
9	1146.5
10	1112.0
11	1165.5
12	1219.0
13	1226.0
14	1233.0
15	1237.5
16	1242.0
17	1326.5
18	1411.0
19	1342.5
20	1274.0
21	1665.5
22	2057.0
23	2111.5
24	2166.0
25	2681.0
26	4088.0
27	4980.0
28	5998.0
29	7016.0
30	8639.0
31	10262.0
32	11638.0
33	13014.0
34	14504.0
35	15994.0
36	16970.0
37	17946.0
38	19224.5
39	20503.0
40	24010.0
41	27517.0
42	29987.0
43	32457.0
44	36314.0
45	40171.0
46	51679.5
47	63188.0
48	64595.0
49	66002.0
50	65034.0
51	64066.0
52	55219.0
53	46372.0
54	41765.0
55	37158.0
56	33790.5
57	30423.0
58	29606.0
59	28789.0
60	27681.0
61	26573.0
62	24988.0
63	23403.0
64	19656.0
65	15909.0
66	13636.5
67	11364.0
68	9101.0
69	6838.0
70	5681.0
71	4524.0
72	3770.5
73	3017.0
74	2271.0
75	1325.5
76	1126.0
77	709.0
78	292.0
79	244.5
80	197.0
81	153.5
82	110.0
83	70.0
84	30.0
85	23.5
86	17.0
87	11.0
88	5.0
89	4.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	642247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.114805297101015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.02374662291507	19.968188077738926
2	11.535286980028669	6.486246949793808
3	4.319768454492409	3.6434834707983907
4	2.08629720433216	2.346233587667394
5	1.233424270832071	1.7338741611581234
6	0.8246571174147005	1.3911044575789726
7	0.6067852526816996	1.1941754465408758
8	0.5010728644303799	1.1270052818496659
9	0.39211957222595156	0.9921928883683651
>10	7.07289562296136	49.03135185171152
>50	0.33662169807123854	5.659660423667677
>100	0.05786554809819475	2.8326459632883294
>500	0.005563995009441802	1.0160169831874144
>1k	0.0038947965066092618	2.5778204566505623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	3650	0.5683171739221826	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	3062	0.4767636127533488	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	2768	0.4309868321689319	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	2760	0.4297412054863627	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1737	0.27045669345283047	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1329	0.20692973264180292	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1173	0.18264001233170415	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	818	0.12736532829269737	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	710	0.11054936807801359	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	709	0.11039366474269247	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	677	0.10541115801241578	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	663	0.10323131131791974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.671100059634377E-4	0.0	0.0	0.2150263060785025	0.0
2	4.671100059634377E-4	0.0	0.0	1.1506476480232684	0.0
3	4.671100059634377E-4	0.0	0.0	1.467348232066479	0.0
4	4.671100059634377E-4	0.0	0.0	2.015891082402876	0.0
5	4.671100059634377E-4	0.0	0.0	3.712434624062082	0.0
6	4.671100059634377E-4	0.0	0.0	4.3785334925659445	0.0
7	4.671100059634377E-4	0.0	0.0	5.208587973162973	0.0
8	4.671100059634377E-4	0.0	0.0	6.447519412313331	0.0
9	4.671100059634377E-4	0.0	0.0	6.820896010413439	0.0
10	4.671100059634377E-4	0.0	0.0	8.172400961000985	0.0
11	4.671100059634377E-4	0.0	0.0	9.629005662930306	0.0
12	4.671100059634377E-4	0.0	0.0	11.349060408223005	0.0
13	4.671100059634377E-4	0.0	0.0	11.833609187742411	0.0
14	4.671100059634377E-4	0.0	0.0	12.037891963683753	0.0
15	4.671100059634377E-4	0.0	0.0	12.477753885965992	0.0
16	4.671100059634377E-4	0.0	0.0	13.213607848693727	0.0
17	4.671100059634377E-4	0.0	0.0	14.081031129767831	0.0
18	4.671100059634377E-4	0.0	0.0	14.960910677667627	0.0
19	4.671100059634377E-4	0.0	0.0	15.637441669638005	0.0
20	6.228133412845837E-4	0.0	0.0	16.22117347375698	0.0
21	6.228133412845837E-4	0.0	0.0	16.949553676389304	0.0
22	6.228133412845837E-4	0.0	0.0	17.765127746801465	0.0
23	6.228133412845837E-4	0.0	0.0	18.49132810273929	0.0
24	6.228133412845837E-4	0.0	0.0	19.103242210551393	0.0
25	6.228133412845837E-4	0.0	0.0	19.609900863686402	0.0
26	6.228133412845837E-4	0.0	0.0	20.057859359405338	0.0
27	6.228133412845837E-4	0.0	0.0	20.527927728739876	0.0
28	6.228133412845837E-4	0.0	0.0	21.01325502493589	0.0
29	6.228133412845837E-4	0.0	0.0	21.518668051388328	0.0
30	6.228133412845837E-4	0.0	0.0	22.0743732551495	0.0
31	6.228133412845837E-4	0.0	0.0	22.597069351822586	0.0
32	6.228133412845837E-4	0.0	0.0	23.144833685482375	0.0
33	7.785166766057296E-4	0.0	0.0	23.644485688527933	0.0
34	7.785166766057296E-4	0.0	0.0	24.11128428782073	0.0
35	7.785166766057296E-4	0.0	0.0	24.648616498014004	0.0
36	7.785166766057296E-4	0.0	0.0	25.166641494627456	0.0
37	0.0010899233472480214	0.0	0.0	25.6728330377565	0.0
38	0.0010899233472480214	0.0	0.0	26.206272664566747	0.0
39	0.0010899233472480214	0.0	0.0	26.747186051472408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	30	2.163999E-6	45.000004	1
CCGATGT	30	2.163999E-6	45.000004	11
TTATCGG	25	3.8888757E-5	45.000004	2
CTTGCGG	50	2.1827873E-11	45.000004	2
TATAGCG	30	2.163999E-6	45.000004	1
CGTAACA	30	2.163999E-6	45.000004	18
ACGTAAC	30	2.163999E-6	45.000004	17
CTTTAGC	30	2.163999E-6	45.000004	19
GTACGAG	25	3.8888757E-5	45.000004	1
GACGTAG	20	7.03096E-4	45.0	1
ATCACGG	20	7.03096E-4	45.0	2
TATGATC	20	7.03096E-4	45.0	33
TAACACG	20	7.03096E-4	45.0	1
CCGAACA	20	7.03096E-4	45.0	14
ACGGTCT	40	6.8066583E-9	45.0	30
GCTACGA	145	0.0	43.44828	10
TACGGGA	165	0.0	40.909092	4
ACGGGTC	50	1.0804797E-9	40.500004	5
TACGGGT	45	1.925946E-8	40.0	4
CGGTCTA	45	1.925946E-8	40.0	31
>>END_MODULE