FastQCFastQC Report
Sat 18 Jun 2016
SRR3550915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792912
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC43120.5438182295134896No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC41440.5226305062856912No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG35410.44658171398591523No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC19630.2475684565248098TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.20481465786871683No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC9240.11653247775289062TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT8340.10518191173799867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG253.889708E-545.01
TTACGCG253.889708E-545.01
CGAAAGG1200.041.2500042
TCGTAAG451.9270374E-840.01
CTTAACG403.4564073E-739.3751
CGTAAGG1950.039.230772
GCGTAAG1050.038.571431
TAGCGCG356.2458384E-638.5714261
TACGGCT5200.038.5096137
CGATGAA3400.038.3823519
CGTACGG659.094947E-1238.076922
ACCACCG4950.037.72727214
CGACCAC7150.037.13286612
TGATACC4100.036.768294
TAACGTC800.036.562532
TACGAAT1050.036.4285712
CGAATAT1050.036.4285714
ACAACGA3400.036.39705713
CACAACG3400.036.39705712
CCGATGA3650.036.36986518
Produced by FastQC (version 0.11.5)