##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792912
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456408781806807	31.0	31.0	33.0	28.0	34.0
2	31.394544413503642	31.0	31.0	34.0	28.0	34.0
3	31.335947747038762	31.0	31.0	34.0	28.0	34.0
4	35.13930171317877	37.0	35.0	37.0	32.0	37.0
5	35.40649025364731	37.0	35.0	37.0	33.0	37.0
6	35.34712553221543	37.0	35.0	37.0	33.0	37.0
7	35.762977480477026	37.0	35.0	37.0	35.0	37.0
8	35.4971030833182	37.0	35.0	37.0	33.0	37.0
9	37.482671469217266	39.0	37.0	39.0	34.0	39.0
10	36.772870129346	39.0	37.0	39.0	32.0	39.0
11	36.88299205964849	39.0	37.0	39.0	33.0	39.0
12	37.03792854692576	39.0	37.0	39.0	33.0	39.0
13	37.12557257299675	39.0	37.0	39.0	33.0	39.0
14	38.1874268519079	40.0	38.0	41.0	33.0	41.0
15	38.32349995964243	40.0	38.0	41.0	34.0	41.0
16	38.33484043626531	40.0	38.0	41.0	34.0	41.0
17	38.21727127348306	40.0	37.0	41.0	34.0	41.0
18	38.18016375083238	40.0	37.0	41.0	34.0	41.0
19	38.0542683172912	40.0	37.0	41.0	34.0	41.0
20	38.15065354036766	40.0	37.0	41.0	34.0	41.0
21	38.04460772443853	40.0	37.0	41.0	34.0	41.0
22	38.19584014367294	40.0	37.0	41.0	34.0	41.0
23	38.26362950743588	40.0	37.0	41.0	34.0	41.0
24	38.214302469883165	40.0	37.0	41.0	34.0	41.0
25	37.89797354561414	40.0	37.0	41.0	33.0	41.0
26	37.96558382266885	40.0	37.0	41.0	34.0	41.0
27	38.0422253667494	40.0	37.0	41.0	34.0	41.0
28	37.970209808906915	40.0	37.0	41.0	34.0	41.0
29	37.992868061020644	40.0	37.0	41.0	34.0	41.0
30	37.74781816897714	40.0	36.0	41.0	33.0	41.0
31	37.72177744819097	40.0	37.0	41.0	33.0	41.0
32	37.68210343644692	40.0	37.0	41.0	33.0	41.0
33	37.70084952680752	40.0	37.0	41.0	33.0	41.0
34	37.66923441693404	40.0	37.0	41.0	33.0	41.0
35	37.621888683737915	40.0	37.0	41.0	33.0	41.0
36	37.53036654761184	40.0	36.0	41.0	33.0	41.0
37	37.53163907217951	40.0	36.0	41.0	33.0	41.0
38	37.371563300845494	40.0	36.0	41.0	33.0	41.0
39	37.33599062695482	40.0	36.0	41.0	33.0	41.0
40	37.18971840506891	40.0	36.0	41.0	32.0	41.0
41	37.05288102588938	40.0	35.0	41.0	32.0	41.0
42	37.07209627297859	40.0	35.0	41.0	32.0	41.0
43	37.04134506729624	39.0	35.0	41.0	32.0	41.0
44	36.97324041003289	39.0	35.0	41.0	32.0	41.0
45	36.920428748713604	39.0	35.0	41.0	32.0	41.0
46	36.76329907984745	39.0	35.0	41.0	31.0	41.0
47	36.70949613576286	39.0	35.0	41.0	31.0	41.0
48	36.61155462396836	39.0	35.0	41.0	31.0	41.0
49	36.590102054200216	39.0	35.0	41.0	31.0	41.0
50	36.46391276711665	39.0	35.0	41.0	31.0	41.0
51	35.23859015880703	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	25.0
10	16.0
11	23.0
12	23.0
13	20.0
14	35.0
15	44.0
16	65.0
17	102.0
18	186.0
19	410.0
20	812.0
21	1303.0
22	1863.0
23	2498.0
24	3222.0
25	4302.0
26	5445.0
27	6489.0
28	7137.0
29	8614.0
30	10557.0
31	13786.0
32	18232.0
33	25706.0
34	45783.0
35	52365.0
36	60404.0
37	96369.0
38	180757.0
39	246267.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65099784087011	21.070812397844907	24.052606089957017	11.225583671327966
2	31.28215993704219	25.788990455435158	25.34518332425288	17.583666283269768
3	32.54383840829752	25.055870008273303	26.026973989547393	16.373317593881794
4	28.551970458260183	25.436113969772183	27.161147769235427	18.850767802732207
5	27.900447969005388	29.86056460237706	23.499959642431946	18.739027786185606
6	26.918119539116574	36.08357547874165	22.35607482293117	14.642230159210607
7	81.51749500575094	5.453694735355247	9.143511511996287	3.885298746897512
8	82.78119135540892	6.4704532154892345	6.833166858365115	3.9151885707367273
9	76.5298040640071	7.867203422321771	8.357800108965433	7.245192404705693
10	39.451162297959925	32.646750206832536	14.995358879673912	12.906728615533627
11	30.011527130375125	26.23229311701677	25.573708053352707	18.182471699255405
12	27.513519785297735	23.51017515184535	28.74379502391186	20.232510038945055
13	25.39209899711443	23.41382145811893	30.417246806707425	20.776832738059202
14	19.893380349900113	29.259362955788284	28.355101196601893	22.49215549770971
15	19.209067336602295	27.970064773896723	32.71220513751841	20.108662751982564
16	22.786765744496236	26.716962285852652	29.938379038279155	20.557892931371953
17	22.499470306919306	26.83311641140505	28.680484089028795	21.98692919264685
18	22.681583832758236	27.579605302984444	27.689579675928726	22.04923118832859
19	24.579902940048832	27.770799281635288	26.272524567669553	21.376773210646327
20	27.89767338620175	26.651002885566115	27.2901658696047	18.161157858627437
21	25.788738220634826	27.011194180438686	27.314380410436467	19.885687188490024
22	24.083378735597393	24.56830013923361	28.047879209798815	23.30044191537018
23	23.62695986439857	28.05418507980709	28.095299352261033	20.223555703533304
24	23.29602780636439	25.61835361301128	28.10210969186997	22.983508888754365
25	22.812367576729827	29.315485198861918	25.99330064370321	21.878846580705048
26	22.696465685977763	29.473762536069575	26.304936739512076	21.524835038440585
27	24.66477995036019	28.56521278527756	26.588196420283715	20.181810844078534
28	20.84127872954376	29.979997780333754	27.997684484532964	21.181039005589522
29	22.79559396250782	28.08748007345077	27.80976955828642	21.30715640575499
30	23.91034566257037	26.885707367274048	27.06214561010554	22.141801360050042
31	23.298045684767036	28.739128680105736	25.515063462275762	22.447762172851466
32	23.743113989950963	29.791704501886716	26.47784369513893	19.987337813023387
33	23.077970821478296	28.334165708174424	25.423880581956134	23.163982888391146
34	23.560117642310875	28.66925964041407	26.504832818774343	21.265789898500717
35	22.8573914885889	27.15938212563311	26.50180600117037	23.481420384607624
36	21.756008232943884	31.069778235163547	24.69063401739411	22.483579514498455
37	23.459602074379	28.62663195915814	26.892517706882984	21.021248259579878
38	21.86484754928668	28.4668412131485	25.659089533264723	24.0092217043001
39	24.598189963072826	25.046285085860724	26.869059870452205	23.48646508061424
40	23.552928950501443	25.8578505559255	28.94671792077809	21.642502572794964
41	20.834468389934823	28.312851867546463	27.63421413725609	23.21846560526263
42	22.89093871703291	28.228731561636096	27.276040720786167	21.604289000544828
43	24.083630970397724	26.921398591520873	26.278956555077993	22.71601388300341
44	23.42870331133846	26.908534616704	26.93855055794338	22.724211514014165
45	22.682214419759063	25.266990536150292	26.950405593558934	25.10038945053171
46	22.400720382589746	27.647204229473132	26.181215569949757	23.77085981798737
47	21.881747280908854	27.653131747280906	28.69650099884981	21.76861997296043
48	22.31773513328087	26.740294004883264	27.190280888673644	23.751689973162218
49	21.750459067336603	26.606609560707874	28.491307988780594	23.15162338317493
50	20.548938595960205	26.774219585527774	27.904735960611017	24.772105857901003
51	20.88378029339952	26.20505175858103	26.670929434792264	26.240238513227194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	563.0
2	946.0
3	2207.0
4	3468.0
5	2489.0
6	1510.0
7	1512.5
8	1515.0
9	1502.0
10	1489.0
11	1403.5
12	1318.0
13	1380.0
14	1442.0
15	1475.5
16	1509.0
17	1540.0
18	1571.0
19	1658.5
20	1746.0
21	2039.5
22	2333.0
23	2819.0
24	3305.0
25	4038.0
26	6105.5
27	7440.0
28	9615.0
29	11790.0
30	13267.5
31	14745.0
32	16656.0
33	18567.0
34	20631.5
35	22696.0
36	25290.0
37	27884.0
38	30370.0
39	32856.0
40	36417.0
41	39978.0
42	41321.5
43	42665.0
44	46538.0
45	50411.0
46	62269.0
47	74127.0
48	70899.0
49	67671.0
50	67596.0
51	67521.0
52	61102.0
53	54683.0
54	50245.5
55	45808.0
56	42351.0
57	38894.0
58	37960.0
59	37026.0
60	36407.5
61	35789.0
62	32409.0
63	29029.0
64	23668.0
65	18307.0
66	14731.0
67	11155.0
68	8919.5
69	6684.0
70	5368.5
71	4053.0
72	3363.5
73	2674.0
74	2092.0
75	1166.0
76	822.0
77	695.0
78	568.0
79	403.0
80	238.0
81	174.5
82	111.0
83	89.5
84	68.0
85	42.5
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	5.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.48035086246283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85197078960357	19.74517367457737
2	8.801559437115326	4.837398829375001
3	3.1609999063402054	2.6059615950732304
4	1.7312505986333635	1.9030149552517461
5	1.1644158616434876	1.599927821389001
6	0.9886547704576204	1.6301147984413837
7	0.8464106367974638	1.628176289104043
8	0.7722932936205439	1.6978312541935663
9	0.6700284168473912	1.6571354384508135
>10	9.853950265368596	56.127514025169
>50	0.11562491837192863	2.064432530122363
>100	0.03639190656821016	1.7251708678945994
>500	0.004145913406504955	0.8053666760746582
>1k	0.002303285225836086	1.972781244883287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC	4312	0.5438182295134896	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	4144	0.5226305062856912	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	3541	0.44658171398591523	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1963	0.2475684565248098	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1624	0.20481465786871683	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	924	0.11653247775289062	TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	834	0.10518191173799867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2611740016546603E-4	0.0	0.0	0.10127227233286921	0.0
2	1.2611740016546603E-4	0.0	0.0	0.5849325019674314	0.0
3	1.2611740016546603E-4	0.0	0.0	0.7603618055975947	0.0
4	1.2611740016546603E-4	0.0	0.0	1.0839790544221806	0.0
5	1.2611740016546603E-4	0.0	0.0	2.281085416792784	0.0
6	1.2611740016546603E-4	0.0	0.0	2.7658807030288353	0.0
7	1.2611740016546603E-4	0.0	0.0	3.2714853602921887	0.0
8	1.2611740016546603E-4	0.0	0.0	3.9749682184151585	0.0
9	1.2611740016546603E-4	0.0	0.0	4.214339043929213	0.0
10	1.2611740016546603E-4	0.0	0.0	5.202216639425308	0.0
11	1.2611740016546603E-4	0.0	0.0	5.99574732126642	0.0
12	1.2611740016546603E-4	0.0	0.0	7.182890409023952	0.0
13	1.2611740016546603E-4	0.0	0.0	7.457952458784834	0.0
14	1.2611740016546603E-4	0.0	0.0	7.5998345339709825	0.0
15	1.2611740016546603E-4	0.0	0.0	7.873004822729382	0.0
16	1.2611740016546603E-4	0.0	0.0	8.29171459127873	0.0
17	1.2611740016546603E-4	0.0	0.0	8.815354036765745	0.0
18	1.2611740016546603E-4	0.0	0.0	9.305194019008415	0.0
19	1.2611740016546603E-4	0.0	0.0	9.74925338499102	0.0
20	1.2611740016546603E-4	0.0	0.0	10.091914361240592	0.0
21	1.2611740016546603E-4	0.0	0.0	10.499904150775874	0.0
22	1.2611740016546603E-4	0.0	0.0	11.025687592065703	0.0
23	1.2611740016546603E-4	0.0	0.0	11.49055632907561	0.0
24	1.2611740016546603E-4	0.0	0.0	11.848099158544706	0.0
25	1.2611740016546603E-4	0.0	0.0	12.171968642169622	0.0
26	1.2611740016546603E-4	0.0	0.0	12.459768549347217	0.0
27	1.2611740016546603E-4	0.0	0.0	12.77203523215691	0.0
28	1.2611740016546603E-4	0.0	0.0	13.095021893980668	0.0
29	2.5223480033093206E-4	0.0	0.0	13.436547813628751	0.0
30	2.5223480033093206E-4	0.0	0.0	13.83016021954517	0.0
31	2.5223480033093206E-4	0.0	0.0	14.194639506023368	0.0
32	2.5223480033093206E-4	0.0	0.0	14.556974796698752	0.0
33	2.5223480033093206E-4	0.0	0.0	14.8774391105192	0.0
34	2.5223480033093206E-4	0.0	0.0	15.197651189539318	0.0
35	2.5223480033093206E-4	0.0	0.0	15.559355893213874	0.0
36	2.5223480033093206E-4	0.0	0.0	15.952968299130294	0.0
37	2.5223480033093206E-4	0.0	0.0	16.332329438828015	0.0
38	2.5223480033093206E-4	0.0	0.0	16.685962628891982	0.0
39	2.5223480033093206E-4	0.0	0.0	17.0620447161854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	25	3.889708E-5	45.0	1
TTACGCG	25	3.889708E-5	45.0	1
CGAAAGG	120	0.0	41.250004	2
TCGTAAG	45	1.9270374E-8	40.0	1
CTTAACG	40	3.4564073E-7	39.375	1
CGTAAGG	195	0.0	39.23077	2
GCGTAAG	105	0.0	38.57143	1
TAGCGCG	35	6.2458384E-6	38.571426	1
TACGGCT	520	0.0	38.509613	7
CGATGAA	340	0.0	38.38235	19
CGTACGG	65	9.094947E-12	38.07692	2
ACCACCG	495	0.0	37.727272	14
CGACCAC	715	0.0	37.132866	12
TGATACC	410	0.0	36.76829	4
TAACGTC	80	0.0	36.5625	32
TACGAAT	105	0.0	36.42857	12
CGAATAT	105	0.0	36.42857	14
ACAACGA	340	0.0	36.397057	13
CACAACG	340	0.0	36.397057	12
CCGATGA	365	0.0	36.369865	18
>>END_MODULE