SRR3550913_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626877
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	11100	1.770682286955814	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	9625	1.5353889199954696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	9464	1.5097060507882727	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	4448	0.7095490821963479	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	2222	0.3544554992446684	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	2015	0.3214346674068438	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1716	0.27373791030776373	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1540	0.24566222719927516	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	1522	0.24279085051772514	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	1476	0.23545288788709745	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1173	0.18711804708100632	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1081	0.1724421218197509	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	655	0.10448620702306832	Illumina Single End Adapter 1 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.030829E-4	45.000004	14
CGTTGAT	20	7.030829E-4	45.000004	25
ACAATCG	20	7.030829E-4	45.000004	41
ATACGGT	30	2.163917E-6	45.000004	28
TTCGCAA	20	7.030829E-4	45.000004	14
ATCGAGC	20	7.030829E-4	45.000004	30
TATCCGG	20	7.030829E-4	45.000004	2
CTCGAAT	105	0.0	42.857147	43
CGATGAA	795	0.0	42.45283	19
CGAATGC	85	0.0	42.35294	45
TACGGCT	1220	0.0	42.23361	7
ACCACCG	1195	0.0	42.17573	14
CCGATGA	785	0.0	42.13376	18
CCACCGA	1145	0.0	41.266376	15
ATAGCGG	120	0.0	41.250004	2
CACCGAT	895	0.0	40.977654	16
TGATACC	995	0.0	40.92965	4
CGACCAC	1800	0.0	40.875	12
ACGGCTG	1270	0.0	40.74803	8
CTACGAA	105	0.0	40.714287	11