##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626877
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298860861062057	31.0	31.0	33.0	28.0	34.0
2	31.223693005166883	31.0	31.0	34.0	28.0	34.0
3	31.18054738010806	31.0	31.0	34.0	28.0	34.0
4	35.03228703557476	35.0	35.0	37.0	32.0	37.0
5	35.28946348326705	35.0	35.0	37.0	33.0	37.0
6	35.20944140557079	37.0	35.0	37.0	33.0	37.0
7	35.570700472341464	37.0	35.0	37.0	33.0	37.0
8	35.15928643099045	37.0	35.0	37.0	32.0	37.0
9	37.04223157014853	39.0	37.0	39.0	33.0	39.0
10	36.44952678117079	38.0	35.0	39.0	32.0	39.0
11	36.623562517048796	38.0	35.0	39.0	32.0	39.0
12	36.70583383981228	39.0	35.0	39.0	33.0	39.0
13	36.69544902748067	39.0	35.0	39.0	33.0	39.0
14	37.688808171299954	39.0	36.0	41.0	33.0	41.0
15	37.74575235652289	40.0	36.0	41.0	33.0	41.0
16	37.85783973570573	39.0	36.0	41.0	33.0	41.0
17	37.61915495384262	39.0	36.0	41.0	33.0	41.0
18	37.623635896675104	39.0	36.0	41.0	33.0	41.0
19	37.524504807163126	39.0	36.0	41.0	32.0	41.0
20	37.50734833149087	39.0	36.0	41.0	32.0	41.0
21	37.52150421853091	39.0	36.0	41.0	33.0	41.0
22	37.66066548940223	39.0	36.0	41.0	33.0	41.0
23	37.714602705155876	39.0	36.0	41.0	33.0	41.0
24	37.617738408013054	39.0	36.0	41.0	33.0	41.0
25	37.418458485476414	39.0	35.0	41.0	33.0	41.0
26	37.441329000744965	39.0	35.0	41.0	33.0	41.0
27	37.48622616557953	39.0	36.0	41.0	33.0	41.0
28	37.367376694311645	39.0	35.0	41.0	32.0	41.0
29	37.40983318896689	40.0	35.0	41.0	33.0	41.0
30	37.137623489137425	39.0	35.0	41.0	32.0	41.0
31	37.175356569151525	39.0	35.0	41.0	32.0	41.0
32	37.088816466388145	39.0	35.0	41.0	32.0	41.0
33	37.07376407173975	39.0	35.0	41.0	32.0	41.0
34	37.04577133951317	40.0	35.0	41.0	32.0	41.0
35	36.9937068994396	40.0	35.0	41.0	32.0	41.0
36	36.89742166326089	40.0	35.0	41.0	31.0	41.0
37	36.95456365443301	40.0	35.0	41.0	32.0	41.0
38	36.77091359229961	39.0	35.0	41.0	31.0	41.0
39	36.793442732784904	39.0	35.0	41.0	31.0	41.0
40	36.59783817240065	39.0	35.0	41.0	31.0	41.0
41	36.50321673948797	39.0	35.0	41.0	30.0	41.0
42	36.554781879060805	39.0	35.0	41.0	31.0	41.0
43	36.55608356982311	39.0	35.0	41.0	31.0	41.0
44	36.54524890847806	39.0	35.0	41.0	31.0	41.0
45	36.50277965214867	39.0	35.0	41.0	31.0	41.0
46	36.36222735879606	39.0	35.0	40.0	30.0	41.0
47	36.28122582260954	39.0	35.0	40.0	31.0	41.0
48	36.253877554927044	39.0	35.0	40.0	30.0	41.0
49	36.257407753036084	39.0	35.0	40.0	30.0	41.0
50	36.113830942912244	39.0	35.0	40.0	30.0	41.0
51	35.02648206905023	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	18.0
10	29.0
11	14.0
12	18.0
13	10.0
14	24.0
15	49.0
16	68.0
17	105.0
18	200.0
19	515.0
20	969.0
21	1690.0
22	2374.0
23	3049.0
24	3780.0
25	4409.0
26	5183.0
27	5916.0
28	6569.0
29	7521.0
30	9793.0
31	12964.0
32	17939.0
33	25140.0
34	45134.0
35	49012.0
36	49215.0
37	74905.0
38	136352.0
39	163881.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.01404422239132	19.249390231257486	21.821505654219248	10.91505989213195
2	30.129993603210835	30.08708247391434	22.621662622811172	17.16126130006365
3	30.715435404393528	29.173027563620934	24.402554249079163	15.708982782906375
4	26.81977485216398	23.560921839531517	31.50043788494394	18.118865423360564
5	30.339604100963985	27.199434657835585	23.21890897257357	19.24205226862686
6	24.83150602111738	37.961673502138375	22.845310962118564	14.361509514625675
7	74.84785691610955	5.520540712133322	15.006133579633643	4.6254687921234945
8	73.79358311120045	11.084471116343398	9.492771309204198	5.629174463251962
9	68.58251299696751	7.02370640492473	10.705289873452049	13.688490724655713
10	42.23874859023381	23.930053264037443	18.841016658770382	14.990181486958368
11	34.22314106276032	23.618827935942775	23.145529346267292	19.012501655029617
12	27.82571381626699	20.892934339591342	28.009481923886188	23.27186992025549
13	26.12793259283719	22.62261974837169	31.737805023952063	19.511642634839053
14	21.52336104211831	28.869778281863905	27.150142691468982	22.456717984548803
15	19.854293585504017	24.486462256551125	34.92949972642161	20.72974443152325
16	22.317615736420382	25.55796432154952	27.562982849905165	24.56143709212493
17	21.7324929770912	26.770163843943866	29.554761141340325	21.942582037624607
18	22.498831509211538	25.45395667730671	28.710576396964637	23.336635416517115
19	22.17324929770912	27.49773879086328	26.097464095827412	24.23154781560019
20	26.25953735740823	26.794411024810287	29.637871544178523	17.308180073602955
21	23.69364325058983	30.328597157018045	27.250959917176736	18.726799675215393
22	22.12204308022148	23.98684271396143	29.870293534457314	24.020820671359772
23	23.248101302169324	28.3380950329969	28.40557238501333	20.008231279820443
24	24.452005736372527	24.245266615300928	27.13801910103577	24.164708547290775
25	20.80105028578174	31.007358700351105	25.0597804673006	23.131810546566552
26	20.729425389669746	24.477369563726217	29.419487395453974	25.37371765115007
27	23.22528980964368	26.660253925411205	27.06639420492377	23.048062060021344
28	17.867300921871436	27.73845586933322	30.774298626365297	23.619944582430048
29	22.31873238290765	23.719645161650533	27.899891047207028	26.061731408234788
30	20.878896498037093	26.454312408973372	31.798582497044876	20.868208595944658
31	22.85583934328425	23.918089194530985	28.6596892213305	24.566382240854267
32	25.25950066759508	25.778103200468355	26.05136254799586	22.91103358394071
33	21.9078064755925	23.26740333430641	28.984952390979412	25.83983779912168
34	21.52399912582532	22.98457273117374	31.44157466297216	24.049853480028776
35	21.954705628057816	23.52630579842617	29.45777241787464	25.06121615564138
36	20.10410335679886	26.3062769889468	28.14236285587125	25.447256798383094
37	20.857520693852223	24.560160924710907	31.846757816924214	22.735560564512657
38	20.477222804473605	25.52558157341871	28.75747554942995	25.239720072677734
39	22.51526216466707	23.191152331318584	28.603697376040277	25.68988812797407
40	23.126705876910464	22.287466281264106	31.422910714542084	23.162917127283343
41	19.76767372227726	24.862772122760923	28.420407831201338	26.94914632376048
42	22.792509535363397	22.90082424462853	29.069498482158384	25.237167737849692
43	22.30022795540433	22.57364682385859	26.737781095813055	28.388344124924032
44	21.48427841506388	22.933685555539604	29.615538614433135	25.96649741496338
45	21.427488965139894	21.39063963106	28.45486435137994	28.727007052420173
46	24.287858702743918	24.668156592122536	27.254150335711792	23.789834369421754
47	18.748494521253768	23.604949615315284	34.55653979967362	23.090016063757325
48	21.151517761857587	23.733523482278024	29.50467157033996	25.610287185524435
49	21.93939161908955	20.90729122299909	32.88029390135545	24.273023256555913
50	20.919255292505547	21.683520052578096	30.353641942518227	27.043582712398127
51	20.12771245395827	21.020391560066805	28.480866262440642	30.37102972353428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	437.5
2	710.0
3	1604.0
4	2498.0
5	1844.0
6	1190.0
7	1139.0
8	1088.0
9	1116.5
10	1145.0
11	1135.0
12	1125.0
13	1142.5
14	1160.0
15	1156.5
16	1153.0
17	1241.0
18	1329.0
19	1248.0
20	1167.0
21	1368.0
22	1569.0
23	1725.0
24	1881.0
25	2405.5
26	3293.0
27	3656.0
28	4698.0
29	5740.0
30	6702.0
31	7664.0
32	9512.5
33	11361.0
34	12958.0
35	14555.0
36	15597.0
37	16639.0
38	18517.5
39	20396.0
40	22881.5
41	25367.0
42	28462.5
43	31558.0
44	34434.5
45	37311.0
46	61054.5
47	84798.0
48	73096.5
49	61395.0
50	61556.5
51	61718.0
52	53918.5
53	46119.0
54	42764.5
55	39410.0
56	36472.0
57	33534.0
58	31458.5
59	29383.0
60	27469.5
61	25556.0
62	22626.0
63	19696.0
64	16265.5
65	12835.0
66	10103.5
67	7372.0
68	5899.5
69	4427.0
70	3710.0
71	2993.0
72	2406.0
73	1819.0
74	1434.5
75	880.5
76	711.0
77	567.5
78	424.0
79	274.5
80	125.0
81	100.5
82	76.0
83	60.0
84	44.0
85	25.5
86	7.0
87	9.5
88	12.0
89	10.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.231663365874656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48497167051086	21.913412626278454
2	10.150195073698859	6.137145611320464
3	3.6024830320275196	3.267271629165943
4	1.7357560101429161	2.0989916553573744
5	1.2084495595882605	1.826672014005589
6	0.9173691749990162	1.6640157648480252
7	0.7806202158669273	1.6519613307879748
8	0.7239817235557189	1.7509737399665775
9	0.6460317505490581	1.7577552965639252
>10	7.619635864249242	46.38008544982944
>50	0.08594292609547591	1.6984514008703422
>100	0.03660532037399118	1.9808851040742248
>500	0.001591535668434399	0.27313151422867826
>1k	0.004774607005303197	2.7574575009592865
>5k	0.001061023778956266	3.061542850916758
>10k+	5.30511889478133E-4	1.7802465108269692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	11100	1.770682286955814	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	9625	1.5353889199954696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	9464	1.5097060507882727	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	4448	0.7095490821963479	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	2222	0.3544554992446684	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	2015	0.3214346674068438	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1716	0.27373791030776373	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1540	0.24566222719927516	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	1522	0.24279085051772514	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	1476	0.23545288788709745	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1173	0.18711804708100632	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1081	0.1724421218197509	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	655	0.10448620702306832	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.785627802583282E-4	0.0	0.0	0.19748690731993676	0.0
2	4.785627802583282E-4	0.0	0.0	1.431859838532918	0.0
3	4.785627802583282E-4	0.0	0.0	1.7625467196914226	0.0
4	4.785627802583282E-4	0.0	0.0	2.3944091105591685	0.0
5	4.785627802583282E-4	0.0	0.0	5.070213135910234	0.0
6	4.785627802583282E-4	0.0	0.0	5.749772283877061	0.0
7	4.785627802583282E-4	0.0	0.0	6.709769221075267	0.0
8	4.785627802583282E-4	0.0	0.0	7.842686842873483	0.0
9	4.785627802583282E-4	0.0	0.0	8.159973966184754	0.0
10	4.785627802583282E-4	0.0	0.0	10.463775190348345	0.0
11	6.380837070111043E-4	0.0	0.0	11.689374470589925	0.0
12	6.380837070111043E-4	0.0	0.0	14.421967945864978	0.0
13	6.380837070111043E-4	0.0	0.0	14.886492884569062	0.0
14	6.380837070111043E-4	0.0	0.0	15.113810205191768	0.0
15	6.380837070111043E-4	0.0	0.0	15.67181440697298	0.0
16	6.380837070111043E-4	0.0	0.0	16.35217115957357	0.0
17	6.380837070111043E-4	0.0	0.0	17.096176761948517	0.0
18	6.380837070111043E-4	0.0	0.0	17.80843769989966	0.0
19	6.380837070111043E-4	0.0	0.0	18.70319057805598	0.0
20	6.380837070111043E-4	0.0	0.0	19.23519286877649	0.0
21	6.380837070111043E-4	0.0	0.0	19.830046404637592	0.0
22	6.380837070111043E-4	0.0	0.0	20.547412012244827	0.0
23	6.380837070111043E-4	0.0	0.0	21.153751054832128	0.0
24	6.380837070111043E-4	0.0	0.0	21.647149281278466	0.0
25	6.380837070111043E-4	0.0	0.0	22.09843398306207	0.0
26	6.380837070111043E-4	0.0	0.0	22.502819532380354	0.0
27	6.380837070111043E-4	0.0	0.0	22.98632746136802	0.0
28	9.571255605166564E-4	0.0	0.0	23.387841634004758	0.0
29	9.571255605166564E-4	0.0	0.0	23.815836280482454	0.0
30	9.571255605166564E-4	0.0	0.0	24.27350181933617	0.0
31	9.571255605166564E-4	0.0	0.0	24.68841574982014	0.0
32	9.571255605166564E-4	0.0	0.0	25.108753391813703	0.0
33	9.571255605166564E-4	0.0	0.0	25.534514745316866	0.0
34	0.0011166464872694325	0.0	0.0	25.96394508013534	0.0
35	0.0011166464872694325	0.0	0.0	26.400394335730933	0.0
36	0.0012761674140222086	0.0	0.0	26.82472000089332	0.0
37	0.0014356883407749845	0.0	0.0	27.229265071138357	0.0
38	0.0014356883407749845	0.0	0.0	27.69315192613543	0.0
39	0.0014356883407749845	0.0	0.0	28.145553274406304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.030829E-4	45.000004	14
CGTTGAT	20	7.030829E-4	45.000004	25
ACAATCG	20	7.030829E-4	45.000004	41
ATACGGT	30	2.163917E-6	45.000004	28
TTCGCAA	20	7.030829E-4	45.000004	14
ATCGAGC	20	7.030829E-4	45.000004	30
TATCCGG	20	7.030829E-4	45.000004	2
CTCGAAT	105	0.0	42.857147	43
CGATGAA	795	0.0	42.45283	19
CGAATGC	85	0.0	42.35294	45
TACGGCT	1220	0.0	42.23361	7
ACCACCG	1195	0.0	42.17573	14
CCGATGA	785	0.0	42.13376	18
CCACCGA	1145	0.0	41.266376	15
ATAGCGG	120	0.0	41.250004	2
CACCGAT	895	0.0	40.977654	16
TGATACC	995	0.0	40.92965	4
CGACCAC	1800	0.0	40.875	12
ACGGCTG	1270	0.0	40.74803	8
CTACGAA	105	0.0	40.714287	11
>>END_MODULE