FastQCFastQC Report
Sat 18 Jun 2016
SRR3550911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789761
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC53100.6723553074917602No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC47100.5963829563627477No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.5957498534366726No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG37620.47634664157890805No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC31660.4008807727907557TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC21240.26894212299670406TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT17520.22183926529671635Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG15850.20069362756580789No Hit
GAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT13580.17195075472199817No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT13280.16815213716554755No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC11880.15042525523544464No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11280.1428280201225434No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT10710.1356106467652872No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT9950.12598748228894566No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGATTGCT9190.11636431781260406No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC8590.10876708269970281No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT8570.10851384152927278No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC8000.10129646817201658TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCA207.031944E-445.040
GTATGCG253.8896935E-545.01
CGACTTT207.031944E-445.020
GATTCGT351.2112832E-745.011
CGATCTA253.8896935E-545.035
CGAATAT2000.043.87514
CTACGAA1950.043.84615311
GCTACGA2000.042.7510
CGTTTTT30900.042.6699071
CATACGG603.6379788E-1241.2500042
TACGAAT2100.040.71428712
GCGATCT501.0804797E-940.59
CGTCATA501.0804797E-940.538
TTTAGCG800.039.3751
TGCTACG403.456389E-739.3751
CCGATGA3500.038.57142618
AATGGGC2050.038.4146354
CGTTCTG1700.038.382351
TAAGGGA6200.038.104844
ACAACGA1250.037.80000313
Produced by FastQC (version 0.11.5)