##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789761
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.363694839324808	31.0	31.0	33.0	28.0	34.0
2	31.286230897701962	31.0	31.0	34.0	28.0	34.0
3	31.175112470734817	31.0	31.0	34.0	28.0	34.0
4	34.986110988007766	35.0	35.0	37.0	32.0	37.0
5	35.321812548353236	35.0	35.0	37.0	33.0	37.0
6	35.27948455292171	37.0	35.0	37.0	33.0	37.0
7	35.625010604474014	37.0	35.0	37.0	35.0	37.0
8	35.39646804539601	37.0	35.0	37.0	33.0	37.0
9	37.339043077589295	39.0	37.0	39.0	34.0	39.0
10	36.577626649074844	38.0	35.0	39.0	32.0	39.0
11	36.799443122666226	39.0	37.0	39.0	33.0	39.0
12	36.98813311875365	39.0	37.0	39.0	33.0	39.0
13	37.086674322991385	39.0	37.0	39.0	33.0	39.0
14	38.10160415619409	40.0	37.0	41.0	33.0	41.0
15	38.22923770609083	40.0	38.0	41.0	34.0	41.0
16	38.23351874807695	40.0	37.0	41.0	34.0	41.0
17	38.12473900331872	40.0	37.0	41.0	33.0	41.0
18	38.05234874854545	39.0	37.0	41.0	34.0	41.0
19	37.876268896539585	39.0	37.0	41.0	33.0	41.0
20	37.94585703776206	39.0	36.0	41.0	34.0	41.0
21	37.82967631979802	39.0	36.0	41.0	33.0	41.0
22	38.01271650537314	40.0	37.0	41.0	34.0	41.0
23	38.103403434709996	40.0	37.0	41.0	34.0	41.0
24	38.005649810512296	40.0	37.0	41.0	34.0	41.0
25	37.68543521394447	39.0	36.0	41.0	33.0	41.0
26	37.805108127648744	40.0	36.0	41.0	33.0	41.0
27	37.908227932247854	40.0	37.0	41.0	34.0	41.0
28	37.81300038872519	40.0	36.0	41.0	33.0	41.0
29	37.83103749108908	40.0	36.0	41.0	33.0	41.0
30	37.547154139037	40.0	36.0	41.0	33.0	41.0
31	37.47797624851062	40.0	36.0	41.0	33.0	41.0
32	37.37586181135812	40.0	36.0	41.0	33.0	41.0
33	37.29665177186516	40.0	36.0	41.0	32.0	41.0
34	37.191475648962154	40.0	36.0	41.0	32.0	41.0
35	37.12934039538544	40.0	36.0	41.0	32.0	41.0
36	37.0125139124368	40.0	36.0	41.0	31.0	41.0
37	37.014636073445004	40.0	36.0	41.0	31.0	41.0
38	36.82774029105008	40.0	35.0	41.0	31.0	41.0
39	36.82267166902392	40.0	35.0	41.0	31.0	41.0
40	36.73264443293604	39.0	35.0	41.0	31.0	41.0
41	36.60043101647207	39.0	35.0	41.0	30.0	41.0
42	36.61672328717169	39.0	35.0	41.0	31.0	41.0
43	36.59075467134994	39.0	35.0	41.0	31.0	41.0
44	36.53341960415872	39.0	35.0	41.0	31.0	41.0
45	36.486514274571675	39.0	35.0	41.0	30.0	41.0
46	36.359940538973184	39.0	35.0	41.0	30.0	41.0
47	36.33710198401795	39.0	35.0	41.0	30.0	41.0
48	36.23336427096299	39.0	35.0	41.0	30.0	41.0
49	36.2247135525811	39.0	35.0	41.0	30.0	41.0
50	36.1201591367515	39.0	35.0	41.0	29.0	41.0
51	34.844897380346715	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	16.0
10	23.0
11	23.0
12	18.0
13	25.0
14	27.0
15	32.0
16	59.0
17	114.0
18	224.0
19	408.0
20	786.0
21	1304.0
22	2042.0
23	2852.0
24	4316.0
25	6410.0
26	8298.0
27	8863.0
28	8966.0
29	9561.0
30	11218.0
31	14449.0
32	19147.0
33	26860.0
34	45910.0
35	54523.0
36	61987.0
37	97100.0
38	182825.0
39	221329.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.69686905278939	21.677190947641122	24.69088749634383	12.935052503225657
2	33.43543173187838	25.635350441462666	24.744194762719353	16.185023063939596
3	29.93449917126827	24.723808848499736	29.509180625531016	15.832511354700978
4	26.882943067586268	24.575789384383377	30.529489301193653	18.011778246836702
5	25.45301679875304	28.50128076721945	27.3603026738469	18.685399760180612
6	24.22631656918992	34.60451959516867	26.59893309494898	14.57023074069244
7	76.76145061607245	5.467223628414166	13.059393917906808	4.711931837606567
8	77.84975454599557	6.140465280002431	10.884052263912753	5.125727910089255
9	72.55612773991119	6.574773887289951	12.626731378227085	8.242366994571777
10	37.6563289400211	25.39806346476972	21.554622221152982	15.390985374056202
11	27.797523554594367	25.23282360106412	26.934984128109647	20.034668716231874
12	25.022506809021973	21.22819435246866	32.049569426699975	21.699729411809397
13	23.370234792551166	23.611320386800564	33.740460721661364	19.27798409898691
14	19.918431019004483	28.091283312293214	30.66218767449899	21.32809799420331
15	18.729463723835437	27.037926663889454	34.99982906220996	19.232780550065147
16	20.659541304268	26.33138379838964	31.48876685478265	21.520308042559712
17	20.298292774649546	27.356250815120013	30.117845778659618	22.22761063157082
18	21.054344288968434	27.00525855290398	30.814638859097883	21.125758299029705
19	21.357727211143622	28.731223749969924	28.42543503667565	21.485614002210795
20	22.855015631311247	27.60594660916404	30.611159578657343	18.927878180867378
21	21.714923882035198	28.752496008286048	30.407806918802017	19.124773190876734
22	20.523424175161853	25.403634770519183	30.08707697645237	23.985864077866594
23	20.433776800829616	27.478439679852514	30.246239052067654	21.841544467250216
24	21.441676659141184	25.621802038844667	30.35817164939773	22.578349652616424
25	19.66164447218842	29.40180636926868	28.237276847046132	22.699272311496767
26	19.352183761922912	27.816390021791403	30.204707500117124	22.62671871616856
27	21.054344288968434	27.624306594020215	29.146286028304765	22.175063088706583
28	19.07374509503508	27.378282796947428	30.930243453399193	22.617728654618297
29	20.418455710018602	25.560011193259736	31.035971642053735	22.98556145466793
30	20.530514927933893	26.81583415742231	29.95298577670966	22.700665137934134
31	21.91650385369751	27.294080107779443	27.90325174324891	22.88616429527414
32	21.94549996771175	28.742872843809707	27.624179973435005	21.687447215043537
33	20.886825254728965	26.79088990213495	28.44012302456059	23.882161818575494
34	20.38743366664092	25.740445527191135	31.59411518168155	22.278005624486394
35	21.263901357499293	25.521771776524798	29.111971849711495	24.102355016264415
36	21.32100724143127	25.825154698699986	29.103361649916877	23.750476409951872
37	21.14779028085712	27.36435453257378	29.648083407511894	21.83977177905721
38	21.490932066789824	27.37423093822055	27.969600929901578	23.165236065088045
39	21.42673543008581	25.578117936945482	30.46250701161491	22.532639621353802
40	21.758228122178735	23.951803140443754	32.81359803788741	21.4763706994901
41	20.142422834249853	25.350960607069734	29.653148230920497	24.853468327759916
42	21.55918056222072	25.0480841672354	29.375342666958737	24.017392603585137
43	21.79038975082335	24.896899188488668	28.641702996222907	24.67100806446507
44	21.07207117089854	25.181668884637247	28.50735855530977	25.23890138915444
45	21.269472663248756	24.00093192750718	28.730210785288207	25.999384623955855
46	22.36056224604659	25.39350512370198	28.891525410852143	23.354407219399288
47	19.763827284456944	24.62808368607718	32.30293721771523	23.305151811750644
48	20.393384834146026	24.370664036335043	30.022880339748355	25.213070789770576
49	20.59825694102393	23.460895131565117	31.839885737583902	24.10096218982705
50	19.871834643645357	23.020255495016848	31.60576427552133	25.502145585816464
51	19.39878013728204	22.618361757544374	29.454987015058986	28.527871090114605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	540.5
2	851.0
3	4320.0
4	7789.0
5	5392.0
6	2995.0
7	2943.5
8	2892.0
9	2881.5
10	2871.0
11	2895.0
12	2919.0
13	2952.5
14	2986.0
15	2914.0
16	2842.0
17	2718.0
18	2594.0
19	2638.5
20	2683.0
21	2782.0
22	2881.0
23	3226.0
24	3571.0
25	4034.0
26	5364.5
27	6232.0
28	7497.5
29	8763.0
30	9789.0
31	10815.0
32	13386.0
33	15957.0
34	18638.0
35	21319.0
36	23799.0
37	26279.0
38	29076.5
39	31874.0
40	34279.0
41	36684.0
42	40918.0
43	45152.0
44	50296.5
45	55441.0
46	73444.5
47	91448.0
48	90575.0
49	89702.0
50	86283.5
51	82865.0
52	69983.5
53	57102.0
54	49011.0
55	40920.0
56	35766.5
57	30613.0
58	28460.0
59	26307.0
60	24584.0
61	22861.0
62	19888.0
63	16915.0
64	14218.5
65	11522.0
66	9427.0
67	7332.0
68	5995.5
69	4659.0
70	3779.5
71	2900.0
72	2212.0
73	1524.0
74	1182.5
75	723.5
76	606.0
77	435.0
78	264.0
79	205.0
80	146.0
81	89.5
82	33.0
83	37.5
84	42.0
85	27.5
86	13.0
87	15.0
88	17.0
89	11.0
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	789761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.220482194124585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35198229001672	27.67416632923434
2	10.596689580535784	7.888277916979954
3	3.868670548710804	4.31981349819674
4	1.9765347279775156	2.94270302595024
5	1.2735296011233168	2.3700692921150504
6	0.9450377268745067	2.1104855931545132
7	0.7764841873098086	2.023078110744882
8	0.6268684932794111	1.8665878073731224
9	0.5147925153321946	1.7244743085531498
>10	4.926982999019288	37.13954953939744
>50	0.09552218170028544	2.3209377740419472
>100	0.0390305612096375	2.4181687886547993
>500	0.003423733439441681	0.9725687286579224
>1k	0.0041084801273300175	3.5524500063937237
>5k	3.423733439441681E-4	0.6766692805520922
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	5310	0.6723553074917602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	4710	0.5963829563627477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4705	0.5957498534366726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	3762	0.47634664157890805	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	3166	0.4008807727907557	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2124	0.26894212299670406	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	1752	0.22183926529671635	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	1585	0.20069362756580789	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	1358	0.17195075472199817	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT	1328	0.16815213716554755	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	1188	0.15042525523544464	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1128	0.1428280201225434	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT	1071	0.1356106467652872	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT	995	0.12598748228894566	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGATTGCT	919	0.11636431781260406	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	859	0.10876708269970281	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT	857	0.10851384152927278	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	800	0.10129646817201658	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5324117043004147E-4	0.0	0.0	0.2313358091878429	0.0
2	2.5324117043004147E-4	0.0	0.0	1.1203389379825035	0.0
3	2.5324117043004147E-4	0.0	0.0	1.5974453030727018	0.0
4	2.5324117043004147E-4	0.0	0.0	2.2942383835109608	0.0
5	2.5324117043004147E-4	0.0	0.0	4.457551081909591	0.0
6	2.5324117043004147E-4	0.0	0.0	5.743383124768126	0.0
7	2.5324117043004147E-4	0.0	0.0	6.891451970912719	0.0
8	2.5324117043004147E-4	0.0	0.0	8.414824231634634	0.0
9	3.7986175564506226E-4	0.0	0.0	8.956507095184493	0.0
10	3.7986175564506226E-4	0.0	0.0	10.659553966326522	0.0
11	3.7986175564506226E-4	0.0	0.0	12.481244325815025	0.0
12	3.7986175564506226E-4	0.0	0.0	14.446522428937362	0.0
13	3.7986175564506226E-4	0.0	0.0	15.083677213739346	0.0
14	3.7986175564506226E-4	0.0	0.0	15.367307324620993	0.0
15	3.7986175564506226E-4	0.0	0.0	15.859734780522208	0.0
16	3.7986175564506226E-4	0.0	0.0	16.701001948690806	0.0
17	3.7986175564506226E-4	0.0	0.0	17.75321901182763	0.0
18	3.7986175564506226E-4	0.0	0.0	18.836837980097776	0.0
19	3.7986175564506226E-4	0.0	0.0	19.67063453373869	0.0
20	3.7986175564506226E-4	0.0	0.0	20.36147644667184	0.0
21	3.7986175564506226E-4	0.0	0.0	21.199451479624848	0.0
22	3.7986175564506226E-4	0.0	0.0	22.151891521612235	0.0
23	3.7986175564506226E-4	0.0	0.0	23.090783160981612	0.0
24	3.7986175564506226E-4	0.0	0.0	23.81847166421234	0.0
25	3.7986175564506226E-4	0.0	0.0	24.450814866776152	0.0
26	3.7986175564506226E-4	0.0	0.0	25.036941555736483	0.0
27	3.7986175564506226E-4	0.0	0.0	25.644846985353798	0.0
28	5.064823408600829E-4	0.0	0.0	26.243762353420845	0.0
29	5.064823408600829E-4	0.0	0.0	26.85723908878762	0.0
30	5.064823408600829E-4	0.0	0.0	27.520351093558684	0.0
31	6.331029260751037E-4	0.0	0.0	28.13661348180019	0.0
32	6.331029260751037E-4	0.0	0.0	28.747051323121806	0.0
33	6.331029260751037E-4	0.0	0.0	29.35343730571654	0.0
34	7.597235112901245E-4	0.0	0.0	29.9296875890301	0.0
35	7.597235112901245E-4	0.0	0.0	30.52417123661462	0.0
36	8.863440965051452E-4	0.0	0.0	31.124985913459895	0.0
37	8.863440965051452E-4	0.0	0.0	31.724407763867802	0.0
38	8.863440965051452E-4	0.0	0.0	32.309521488146416	0.0
39	8.863440965051452E-4	0.0	0.0	32.90096624168578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	20	7.031944E-4	45.0	40
GTATGCG	25	3.8896935E-5	45.0	1
CGACTTT	20	7.031944E-4	45.0	20
GATTCGT	35	1.2112832E-7	45.0	11
CGATCTA	25	3.8896935E-5	45.0	35
CGAATAT	200	0.0	43.875	14
CTACGAA	195	0.0	43.846153	11
GCTACGA	200	0.0	42.75	10
CGTTTTT	3090	0.0	42.669907	1
CATACGG	60	3.6379788E-12	41.250004	2
TACGAAT	210	0.0	40.714287	12
GCGATCT	50	1.0804797E-9	40.5	9
CGTCATA	50	1.0804797E-9	40.5	38
TTTAGCG	80	0.0	39.375	1
TGCTACG	40	3.456389E-7	39.375	1
CCGATGA	350	0.0	38.571426	18
AATGGGC	205	0.0	38.414635	4
CGTTCTG	170	0.0	38.38235	1
TAAGGGA	620	0.0	38.10484	4
ACAACGA	125	0.0	37.800003	13
>>END_MODULE