FastQCFastQC Report
Sat 18 Jun 2016
SRR3550910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601001
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC77561.2905136597110487No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG71261.185688542947516No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC64511.0733759178437308No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC30850.5133102939928552TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC17510.2913472689729302TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT17310.2880194874883736No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT14460.24059860133344205No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.1975038311084341No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT11170.185856595912486No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT10370.1725454699742596TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA10330.17187991367734828No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC10180.16938407756393084No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6040.10049900083360926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAG302.163766E-645.0000041
CGACGGT406.8048394E-945.028
AGGAACG253.8885773E-545.01
TAACGCG207.030598E-445.01
TATTGCG207.030598E-445.01
TAACCGT207.030598E-445.035
TATAGCG253.8885773E-545.01
ACCGTAA207.030598E-445.037
ACGTACG207.030598E-445.01
CCAACGC207.030598E-445.032
GACGAAT253.8885773E-545.020
AGGGCGT253.8885773E-545.06
CCACCGG253.8885773E-545.015
AGTCGAT207.030598E-445.028
TACGGCT8550.041.8421027
TGATACC9500.041.447374
ACCACCG8050.041.3664614
CCACCGA7450.041.07382615
TCACGAC451.9255822E-840.00000425
TACGGGT451.9255822E-840.0000044