##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902484
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36763421844598	31.0	31.0	33.0	28.0	34.0
2	31.287391244609324	31.0	31.0	34.0	28.0	34.0
3	31.21113504505343	31.0	31.0	34.0	28.0	34.0
4	35.08035377912517	35.0	35.0	37.0	32.0	37.0
5	35.31150025928437	35.0	35.0	37.0	33.0	37.0
6	35.21225861067897	37.0	35.0	37.0	33.0	37.0
7	35.58732675593141	37.0	35.0	37.0	33.0	37.0
8	35.21791854481631	37.0	35.0	37.0	32.0	37.0
9	37.13647333359926	39.0	37.0	39.0	34.0	39.0
10	36.56974749690853	38.0	35.0	39.0	32.0	39.0
11	36.69221171788087	39.0	35.0	39.0	32.0	39.0
12	36.77448242849735	39.0	35.0	39.0	33.0	39.0
13	36.74939057091317	39.0	35.0	39.0	33.0	39.0
14	37.71301541079953	40.0	37.0	41.0	33.0	41.0
15	37.78333798715545	40.0	37.0	41.0	33.0	41.0
16	37.95673496704651	40.0	37.0	41.0	33.0	41.0
17	37.734239055761655	40.0	36.0	41.0	33.0	41.0
18	37.72142996440934	39.0	36.0	41.0	33.0	41.0
19	37.59390083369899	39.0	36.0	41.0	33.0	41.0
20	37.481323768620825	39.0	36.0	41.0	32.0	41.0
21	37.560415475509814	39.0	36.0	41.0	33.0	41.0
22	37.69420842917991	39.0	36.0	41.0	33.0	41.0
23	37.74746809915744	40.0	36.0	41.0	33.0	41.0
24	37.66413698192987	40.0	36.0	41.0	33.0	41.0
25	37.47141001945741	39.0	35.0	41.0	33.0	41.0
26	37.44863066824453	39.0	35.0	41.0	33.0	41.0
27	37.497893591465335	39.0	36.0	41.0	33.0	41.0
28	37.40467531834359	40.0	35.0	41.0	33.0	41.0
29	37.44242003182328	40.0	35.0	41.0	33.0	41.0
30	37.19379180129509	39.0	35.0	41.0	32.0	41.0
31	37.17237757123672	39.0	35.0	41.0	32.0	41.0
32	37.02255220037142	39.0	35.0	41.0	32.0	41.0
33	36.98007388496638	40.0	35.0	41.0	31.0	41.0
34	36.850790706538845	40.0	35.0	41.0	31.0	41.0
35	36.77691460458025	40.0	35.0	41.0	31.0	41.0
36	36.68981832364895	40.0	35.0	41.0	31.0	41.0
37	36.701904964520146	40.0	35.0	41.0	31.0	41.0
38	36.53876633823979	39.0	35.0	41.0	30.0	41.0
39	36.52413449989141	39.0	35.0	41.0	30.0	41.0
40	36.337005420594714	39.0	35.0	41.0	30.0	41.0
41	36.168550356571416	39.0	35.0	41.0	29.0	41.0
42	36.24975844447104	39.0	35.0	41.0	30.0	41.0
43	36.21844819409541	39.0	35.0	41.0	30.0	41.0
44	36.2300949379712	39.0	35.0	41.0	30.0	41.0
45	36.24950248425457	39.0	35.0	41.0	30.0	41.0
46	36.129111430230346	39.0	35.0	40.0	30.0	41.0
47	36.01594598907017	39.0	35.0	40.0	30.0	41.0
48	35.944886557545615	38.0	35.0	40.0	29.0	41.0
49	35.98171158713063	39.0	35.0	40.0	30.0	41.0
50	35.85558525137288	38.0	35.0	40.0	29.0	41.0
51	34.6040915960837	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	11.0
9	22.0
10	37.0
11	32.0
12	26.0
13	30.0
14	35.0
15	48.0
16	87.0
17	171.0
18	354.0
19	940.0
20	2007.0
21	3324.0
22	4372.0
23	5008.0
24	5956.0
25	7487.0
26	8968.0
27	9733.0
28	10155.0
29	11480.0
30	13929.0
31	18353.0
32	25040.0
33	36041.0
34	62208.0
35	63763.0
36	69732.0
37	105355.0
38	190703.0
39	247036.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.75351585180457	18.84797957636922	22.15651468613294	12.241989885693265
2	30.9486927192061	30.901489666298794	22.50942953005261	15.640388084442494
3	29.50401336755001	30.207516144330537	25.569317572389096	14.719152915730364
4	26.60157964019307	22.72383776332877	33.03050247982235	17.644080116655807
5	30.50979297139894	27.099760217355655	23.76164009555848	18.628806715686927
6	24.89983201918261	37.62858953732144	23.74391124939611	13.72766719409984
7	74.63256966328488	5.162418391905009	15.949978060552874	4.2550338842572275
8	74.67412164647794	10.885954764849016	9.851809007140293	4.588114581532747
9	69.31679675207538	6.70006338062503	10.769609211908467	13.21353065539112
10	41.781682556145036	25.991374916342007	18.066469876474265	14.160472651038688
11	32.32810775592698	22.83375660953546	26.334760505449406	18.503375129088163
12	26.997486936056486	20.781531860952658	29.514096648804856	22.706884554186
13	25.84378227204028	22.253580118871913	33.42785024443647	18.47478736465134
14	20.703524937838232	28.61280643202539	29.459358836278536	21.22430979385784
15	18.614402028180002	24.781491971048794	35.192202853457786	21.411903147313417
16	22.57026163344724	25.798795324903267	27.58852234499448	24.042420696655007
17	22.344218844877027	27.21743543375838	29.594652093555123	20.84369362780947
18	22.763838472482615	25.108589182744513	28.798848511441754	23.328723833331118
19	22.53569038343062	26.10827449572513	27.17477539768018	24.181259723164068
20	25.116899579383123	26.823965854242292	30.237655182806567	17.821479383568022
21	23.3914396266305	28.932036468236554	29.00228702115494	18.674236883977997
22	21.83119035905346	22.416463892988684	32.38672375355131	23.36562199440655
23	22.83996170569229	26.72767605852292	30.44840684156173	19.983955394223056
24	23.003621116828665	25.86106789704859	28.01523351106502	23.12007747505773
25	21.42121079154866	31.35723181796021	26.272598738592595	20.94895865189854
26	21.12547147650263	25.135736478430644	29.988897310090817	23.74989473497591
27	24.098931393797564	26.847567380695946	27.38752155162862	21.665979673877874
28	19.360897256904277	27.61245628731368	30.32297525496297	22.703671200819073
29	22.023326729338137	24.588469158455993	30.809188860965957	22.579015251239912
30	21.75484551526675	29.130931961120645	29.16683287459944	19.947389649013168
31	25.804778810483064	24.374504146333898	26.082567668789697	23.73814937439334
32	25.193022812592798	26.613214195487124	26.92446625092522	21.269296740994854
33	23.829120516264	25.59214346182315	27.000589484134903	23.578146537777954
34	25.119558906307482	24.16674423036863	29.616369930104025	21.09732693321987
35	24.671462319553587	26.337530637662276	26.77676280133498	22.214244241449155
36	23.82934212684103	29.128715855350347	27.47937913580739	19.562562882001234
37	22.683172222443833	28.343660386222915	27.283918606867267	21.689248784465985
38	24.21383647798742	25.833034159054343	26.619640902220983	23.333488460737254
39	24.8877542427345	24.46680495166673	26.403459784328586	24.241981021270185
40	24.322536466020452	23.881088196577448	30.633894894535523	21.162480442866578
41	23.097140780335163	27.595392272882403	26.33609016891158	22.971376777870855
42	23.85493814848795	24.72176792053931	29.740471853240614	21.682822077732126
43	23.347449927090118	26.272487933304078	27.277048678979348	23.10301346062645
44	23.043511020693998	24.059373905797777	28.888822405715782	24.00829266779245
45	22.381892642972065	22.855252835507333	27.49034885937036	27.27250566215024
46	24.97396075719902	25.960681851423406	26.92158531342384	22.143772077953734
47	20.605683868079655	25.34460444728106	33.167790232292205	20.881921452347076
48	22.42765522712868	26.473599531958463	27.19915256115344	23.89959267975942
49	22.143107246222648	23.822139783087568	31.779399967201634	22.25535300348815
50	22.287264926580416	24.232008545303852	29.517974833902873	23.962751694212862
51	21.723376813328546	23.44440455454058	27.93168632352485	26.900532308606024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	285.0
1	769.5
2	1254.0
3	4487.5
4	7721.0
5	5269.0
6	2817.0
7	2648.5
8	2480.0
9	2414.5
10	2349.0
11	2347.5
12	2346.0
13	2271.0
14	2196.0
15	2224.5
16	2253.0
17	2116.5
18	1980.0
19	2273.0
20	2566.0
21	2717.0
22	2868.0
23	3018.0
24	3168.0
25	4245.5
26	6087.0
27	6851.0
28	8762.0
29	10673.0
30	12652.5
31	14632.0
32	16183.5
33	17735.0
34	20514.5
35	23294.0
36	24770.0
37	26246.0
38	29156.5
39	32067.0
40	35802.0
41	39537.0
42	42143.0
43	44749.0
44	47832.0
45	50915.0
46	86148.0
47	121381.0
48	99972.0
49	78563.0
50	78273.0
51	77983.0
52	70224.5
53	62466.0
54	58358.0
55	54250.0
56	50081.5
57	45913.0
58	43658.0
59	41403.0
60	37986.0
61	34569.0
62	30444.5
63	26320.0
64	22868.0
65	19416.0
66	16113.0
67	12810.0
68	10489.5
69	8169.0
70	6717.5
71	5266.0
72	4341.5
73	3417.0
74	2702.0
75	1599.0
76	1211.0
77	937.0
78	663.0
79	432.5
80	202.0
81	159.0
82	116.0
83	83.0
84	50.0
85	31.5
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	902484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.487458746557454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28713695108043	20.592768318006524
2	8.592365603296662	4.895493213185058
3	3.1671035178689038	2.706681924341021
4	1.8315395114488486	2.0870362510035623
5	1.273142434115322	1.8134296285175988
6	1.0672121284150082	1.8241296889228993
7	0.9391370811202621	1.8727540199043091
8	0.8523300948968698	1.9424574733459181
9	0.7626131442363127	1.9552419437412982
>10	9.12560929211151	48.95360108252997
>50	0.06186930149069085	1.1922525958941574
>100	0.032500960914634454	1.7717578033520858
>500	0.0027410448964149407	0.5208296407385801
>1k	0.003524200581104924	2.827584008019175
>5k	0.0	0.0
>10k+	0.0011747335270349745	5.043982408497832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	16721	1.8527752292561421	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	15112	1.674489520035812	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	13384	1.4830179814822202	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	4418	0.4895377646584316	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.46726590166695475	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT	3835	0.42493828145429724	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCTGGAT	3041	0.33695888237353794	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCT	2729	0.3023876323569171	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTC	2222	0.24620935107990835	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTA	2044	0.22648600972427213	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTT	1512	0.16753759623439307	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1330	0.1473710337246976	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	930	0.10304891831877352	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	909	0.1007220072599625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2161057702962047E-4	0.0	0.0	0.10681629812827706	0.0
2	2.2161057702962047E-4	0.0	0.0	0.8400148922307764	0.0
3	2.2161057702962047E-4	0.0	0.0	1.0722627769578186	0.0
4	2.2161057702962047E-4	0.0	0.0	1.7136037868815404	0.0
5	2.2161057702962047E-4	0.0	0.0	4.22766497799407	0.0
6	2.2161057702962047E-4	0.0	0.0	4.8882861081193685	0.0
7	2.2161057702962047E-4	0.0	0.0	5.6526209882945295	0.0
8	2.2161057702962047E-4	0.0	0.0	6.505378488704509	0.0
9	2.2161057702962047E-4	0.0	0.0	6.786159089801038	0.0
10	2.2161057702962047E-4	0.0	0.0	8.822206266260675	0.0
11	2.2161057702962047E-4	0.0	0.0	9.597178454133259	0.0
12	2.2161057702962047E-4	0.0	0.0	11.8996015441825	0.0
13	2.2161057702962047E-4	0.0	0.0	12.224371844819409	0.0
14	2.2161057702962047E-4	0.0	0.0	12.404541243944491	0.0
15	3.324158655444307E-4	0.0	0.0	12.841114080692844	0.0
16	3.324158655444307E-4	0.0	0.0	13.265276725127537	0.0
17	3.324158655444307E-4	0.0	0.0	13.740298996990528	0.0
18	3.324158655444307E-4	0.0	0.0	14.171331569313141	0.0
19	3.324158655444307E-4	0.0	0.0	14.834833636939823	0.0
20	3.324158655444307E-4	0.0	0.0	15.209355512119883	0.0
21	3.324158655444307E-4	0.0	0.0	15.578891149316775	0.0
22	3.324158655444307E-4	0.0	0.0	16.10444063274252	0.0
23	3.324158655444307E-4	0.0	0.0	16.505666582454648	0.0
24	3.324158655444307E-4	0.0	0.0	16.838082447999078	0.0
25	3.324158655444307E-4	0.0	0.0	17.135816258238375	0.0
26	3.324158655444307E-4	0.0	0.0	17.413051090102428	0.0
27	3.324158655444307E-4	0.0	0.0	17.778043710470214	0.0
28	3.324158655444307E-4	0.0	0.0	18.0861932178299	0.0
29	3.324158655444307E-4	0.0	0.0	18.39212661941929	0.0
30	3.324158655444307E-4	0.0	0.0	18.767867352773013	0.0
31	3.324158655444307E-4	0.0	0.0	19.068260489936662	0.0
32	4.4322115405924094E-4	0.0	0.0	19.395911728074957	0.0
33	4.4322115405924094E-4	0.0	0.0	19.70162351908732	0.0
34	5.540264425740512E-4	0.0	0.0	20.019634697124825	0.0
35	5.540264425740512E-4	0.0	0.0	20.35748002180648	0.0
36	5.540264425740512E-4	0.0	0.0	20.66984013012973	0.0
37	5.540264425740512E-4	0.0	0.0	20.99294835143892	0.0
38	6.648317310888614E-4	0.0	0.0	21.36636217373383	0.0
39	6.648317310888614E-4	0.0	0.0	21.887811861484526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACA	20	7.032481E-4	45.0	11
TATCTCG	20	7.032481E-4	45.0	20
ACGCGCC	20	7.032481E-4	45.0	24
CGATGAA	1390	0.0	43.057552	19
TACGGCT	1625	0.0	42.923077	7
CCGATGA	1395	0.0	42.580643	18
CGATCGA	155	0.0	42.09677	41
GATACCT	1520	0.0	42.039474	5
GATGAAT	1510	0.0	41.87086	20
TGATACC	1530	0.0	41.76471	4
ACGGCTG	1680	0.0	41.651787	8
ACCACCG	2015	0.0	40.421833	14
CGACCAC	2850	0.0	40.42105	12
CGGCTGT	1735	0.0	40.331413	9
CGTTTTT	2380	0.0	40.27311	1
CACCGAT	1540	0.0	40.032467	16
CCACCGA	1945	0.0	40.025707	15
TTAACGG	45	1.927583E-8	40.000004	2
CGATAAG	45	1.927583E-8	40.000004	10
CGGCGAA	270	0.0	40.0	31
>>END_MODULE