FastQCFastQC Report
Sat 18 Jun 2016
SRR3550906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761040
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC90161.1846946284032376No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC85111.1183380637023022No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG77241.01492694207926No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC36500.47960685377903917TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT19390.2547829286239882No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT16520.2170713760117734No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT16410.21562598549353515No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC16380.2152317880794702TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.18698097340481445No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC14140.1857983811626196No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA13610.17883422684747188No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC13170.17305266477451908No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT12070.15859875959213707TruSeq Adapter, Index 16 (95% over 23bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9190.12075580784190056No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8120.10669610007358352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACG453.8380676E-1045.0000041
GATCGCT207.0317835E-445.09
TCGATTG207.0317835E-445.019
TCGATTA253.8895585E-545.041
CTACGAA750.045.011
TATAGCG207.0317835E-445.01
CTCGAAC207.0317835E-445.013
TTTGCGC207.0317835E-445.013
TACGCGG600.044.9999962
TTTTGCG302.164532E-644.9999961
CGTAAGG1150.043.0434762
CGAATAT800.042.187514
TACGGCT10350.041.3043487
ACCACCG9650.040.80311214
CACCGAT6950.040.79136716
CCTCGTG2050.040.60975615
ACGGCTG10550.040.5213248
CACGACG501.0804797E-940.526
CGTGTAC2000.040.518
CGCGCAA451.9268555E-840.00000418
Produced by FastQC (version 0.11.5)