FastQCFastQC Report
Sat 18 Jun 2016
SRR3550905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394649
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC19510.49436334565652007No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC19160.48549470542177986No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT11730.2972261427242942Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC11580.2934252969094056No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC10600.2685931042521329No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG9780.24781514713074151No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.20296516651505514No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6540.16571687752914616No Hit
TCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC5040.12770841938025942No Hit
CGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTG4130.10464995476993481No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4080.10338300616497191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTC700.045.00000436
TATTGCG253.8861333E-545.0000041
GCCCCGT207.027651E-445.016
CCGATTT207.027651E-445.011
CCTAGTG207.027651E-445.08
CGACGGT207.027651E-445.028
ACGTTAT207.027651E-445.040
GCAACTC207.027651E-445.038
ACCGATA406.7957444E-945.010
CACGCAT207.027651E-445.011
GTTAGAC207.027651E-445.017
CGATTGA207.027651E-445.011
TCGATAG207.027651E-445.01
TACGCAC207.027651E-445.039
CATAAGA207.027651E-445.038
CGATAGG750.045.02
TCCGGTC207.027651E-445.015
CATATCC302.1618653E-644.9999963
TCGACGG302.1618653E-644.9999962
AAGCTAC950.042.631588