##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394649
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426665213898932	31.0	31.0	33.0	28.0	34.0
2	31.330942178999567	31.0	31.0	34.0	28.0	34.0
3	31.27171993340918	31.0	31.0	34.0	28.0	34.0
4	35.0968556869522	37.0	35.0	37.0	32.0	37.0
5	35.38666764644025	37.0	35.0	37.0	33.0	37.0
6	35.341196861008136	37.0	35.0	37.0	33.0	37.0
7	35.70680275383949	37.0	35.0	37.0	35.0	37.0
8	35.39379296539457	37.0	35.0	37.0	33.0	37.0
9	37.31470496567836	39.0	37.0	39.0	34.0	39.0
10	36.746220058837096	39.0	35.0	39.0	32.0	39.0
11	36.88275404219952	39.0	37.0	39.0	33.0	39.0
12	36.963942642702754	39.0	37.0	39.0	33.0	39.0
13	36.96029383072046	39.0	37.0	39.0	33.0	39.0
14	37.94957037772806	40.0	37.0	41.0	33.0	41.0
15	38.141903311550266	40.0	37.0	41.0	33.0	41.0
16	38.144313047796906	40.0	37.0	41.0	34.0	41.0
17	38.08895499545165	40.0	37.0	41.0	34.0	41.0
18	38.05059432559059	40.0	37.0	41.0	34.0	41.0
19	37.9137385372825	39.0	37.0	41.0	34.0	41.0
20	38.070639986418314	40.0	37.0	41.0	34.0	41.0
21	37.934220028430325	40.0	36.0	41.0	34.0	41.0
22	38.08810867378354	40.0	37.0	41.0	34.0	41.0
23	38.181008947191046	40.0	37.0	41.0	34.0	41.0
24	38.10572432718694	40.0	37.0	41.0	34.0	41.0
25	37.78289061925914	40.0	36.0	41.0	33.0	41.0
26	37.88871123454006	40.0	36.0	41.0	34.0	41.0
27	37.97181292743679	40.0	37.0	41.0	34.0	41.0
28	37.90802459907411	40.0	36.0	41.0	34.0	41.0
29	37.92350164323234	40.0	36.0	41.0	34.0	41.0
30	37.6879657619809	40.0	36.0	41.0	33.0	41.0
31	37.61761970763894	40.0	36.0	41.0	33.0	41.0
32	37.57765254694678	40.0	36.0	41.0	33.0	41.0
33	37.586341280479616	40.0	36.0	41.0	33.0	41.0
34	37.569574482641535	40.0	36.0	41.0	33.0	41.0
35	37.53513121786702	40.0	36.0	41.0	33.0	41.0
36	37.38074592866066	40.0	36.0	41.0	33.0	41.0
37	37.39482679545622	40.0	36.0	41.0	33.0	41.0
38	37.232358374150195	40.0	35.0	41.0	33.0	41.0
39	37.20062637939029	39.0	35.0	41.0	33.0	41.0
40	37.103687073830166	39.0	35.0	41.0	32.0	41.0
41	36.96762692924599	39.0	35.0	41.0	32.0	41.0
42	36.963321837886326	39.0	35.0	41.0	32.0	41.0
43	36.8884401075386	39.0	35.0	41.0	32.0	41.0
44	36.81815486672968	39.0	35.0	41.0	31.0	41.0
45	36.7865318295498	39.0	35.0	41.0	31.0	41.0
46	36.66055912975834	39.0	35.0	41.0	31.0	41.0
47	36.637842741271356	39.0	35.0	41.0	31.0	41.0
48	36.52853801732679	39.0	35.0	40.0	31.0	41.0
49	36.51022807608786	39.0	35.0	40.0	31.0	41.0
50	36.38234228390291	39.0	35.0	40.0	31.0	41.0
51	35.122587413119	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	14.0
10	5.0
11	10.0
12	12.0
13	14.0
14	7.0
15	22.0
16	34.0
17	63.0
18	106.0
19	176.0
20	320.0
21	433.0
22	688.0
23	1081.0
24	1444.0
25	2132.0
26	2597.0
27	3408.0
28	3711.0
29	4314.0
30	5457.0
31	7100.0
32	9576.0
33	13899.0
34	26683.0
35	29836.0
36	30041.0
37	48689.0
38	90147.0
39	112604.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46900663627679	22.035276916956587	23.24571961413813	11.249996832628488
2	32.46606478161607	25.577411826711838	24.56435972218351	17.392163669488582
3	32.0791386776604	24.042123507217806	27.104338285413114	16.774399529708678
4	30.234461508834432	25.220132320112302	25.78873885401965	18.756667317033617
5	25.56575589954618	30.736426546120732	24.416633514844836	19.281184039488252
6	26.461235173533947	35.45454315100254	23.71905161295227	14.365170062511245
7	79.01375652795268	6.035996543764206	9.919954187138444	5.030292741144662
8	78.26879074823451	6.395556557852674	9.406333222686488	5.92931947122633
9	71.98320532929262	8.40493704532382	11.81049489546407	7.801362729919497
10	40.507894356757525	26.98119088101072	18.343388682094723	14.167526080137034
11	30.962956956688092	26.205311555331445	23.759593968311084	19.072137519669376
12	30.629242694140867	21.856383773935825	28.644947789047993	18.869425742875315
13	24.775940139212313	24.924426515713964	29.64279651031676	20.656836834756962
14	18.204024335548805	30.315292829831066	28.447050416952784	23.033632417667345
15	17.797080443634723	27.12891708834939	35.61772613132176	19.456276336694124
16	20.152084510539744	24.86006552658185	33.51509822652534	21.472751736353064
17	19.405091613053624	25.761879543594436	29.580462638952586	25.25256620439935
18	21.124594259709262	27.022746795253504	29.49912453851397	22.353534406523266
19	23.34884923058211	27.640764324754404	26.631513066040963	22.378873378622526
20	26.94292903314084	26.336060651363617	27.714247343842253	19.006762971653295
21	24.211894620282834	27.530539796122632	27.440333055449273	20.81723252814526
22	21.890591386269826	25.615673674581718	27.1833958783628	25.310339060785658
23	21.45957547086145	27.927601488918004	28.15869291446323	22.454130125757317
24	23.373681423239383	24.042123507217806	29.372176288296686	23.21201878124612
25	21.059219711693174	27.846516778200375	26.01704299263396	25.077220517472487
26	20.95558331580721	29.776586283000846	27.127650139744432	22.140180261447515
27	23.77150328519773	28.594269844849475	26.869952793494978	20.764274076457813
28	20.432840321399524	28.95256291033298	28.097119212262033	22.517477556005463
29	21.517094937526764	28.61352746364491	27.604782984373454	22.264594614454868
30	23.77150328519773	26.336820820526597	27.33112208570147	22.560553808574202
31	23.937980331889857	28.18631239405142	24.09533534862625	23.780371925432473
32	24.75516218209092	30.263094547306597	24.169071757435088	20.8126715131674
33	24.236980202661098	27.521164376445906	26.792668928592246	21.449186492300754
34	22.001576084064574	27.94103114413061	26.54941479643937	23.507977975365453
35	23.454259354515024	26.32567167280292	25.859181196455584	24.36088777622647
36	23.632138938651813	28.122711574082288	27.021986626090527	21.223162861175375
37	25.203915377968777	27.711206667190343	26.680163892471537	20.404714062369344
38	23.421318690785988	27.46643219671151	26.189348002908915	22.92290110959359
39	22.768079990067122	25.38002123405862	29.206459410767543	22.645439365106714
40	24.463764002949457	24.018304873444503	29.467957602831884	22.04997352077416
41	21.090893426817246	26.181492921558142	28.120431066593355	24.607182585031257
42	23.848280370658482	25.908338802328146	28.172629349117823	22.07075147789555
43	24.983466320705233	25.32680939265018	28.164774267767058	21.524950018877533
44	22.78150964527973	26.1386700587104	27.40105764869542	23.67876264731445
45	22.893761291679443	24.93810956064756	25.982075211136983	26.18605393653601
46	23.49530848931582	25.819399010259747	27.142600133282997	23.542692367141434
47	21.560171190095502	25.32503566460323	30.52281901132399	22.59197413397728
48	22.608191076120807	23.944821854356658	28.591735947639545	24.85525112188299
49	22.002589642948543	23.940514229099783	30.341898750535286	23.71499737741639
50	20.207323469716126	24.441212317781115	29.852096419856633	25.499367792646122
51	20.473889456200318	23.466928840564655	26.746298609650598	29.312883093584425
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	498.0
2	725.0
3	1261.5
4	1798.0
5	1349.5
6	901.0
7	832.0
8	763.0
9	750.0
10	737.0
11	753.0
12	769.0
13	777.5
14	786.0
15	809.5
16	833.0
17	867.5
18	902.0
19	935.0
20	968.0
21	1275.0
22	1582.0
23	1621.5
24	1661.0
25	1833.5
26	2477.5
27	2949.0
28	3775.0
29	4601.0
30	5583.5
31	6566.0
32	7068.5
33	7571.0
34	8834.5
35	10098.0
36	10637.0
37	11176.0
38	12254.5
39	13333.0
40	15133.0
41	16933.0
42	19134.0
43	21335.0
44	24147.5
45	26960.0
46	31848.0
47	36736.0
48	39341.0
49	41946.0
50	41736.0
51	41526.0
52	35466.5
53	29407.0
54	25748.0
55	22089.0
56	19910.5
57	17732.0
58	17771.0
59	17810.0
60	16974.5
61	16139.0
62	14637.0
63	13135.0
64	10925.0
65	8715.0
66	6948.5
67	5182.0
68	4169.0
69	3156.0
70	2718.5
71	2281.0
72	1724.5
73	1168.0
74	910.5
75	495.0
76	337.0
77	269.5
78	202.0
79	162.0
80	122.0
81	96.5
82	71.0
83	40.0
84	9.0
85	6.0
86	3.0
87	3.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.14071475223322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57997328985617	22.290515301918838
2	11.61716550226248	7.2353367427088005
3	4.2695865845293275	3.988739338163684
4	2.0275993028842483	2.5256356609178123
5	1.1560209258852527	1.79996589503026
6	0.8032652682533196	1.5008552753431637
7	0.6231136539839459	1.3582943189855066
8	0.5253474530451769	1.3087756144873672
9	0.41294306994789765	1.1573408115143073
>10	6.7667041840120525	48.56553149439639
>50	0.1620816130697227	3.1983378437455454
>100	0.04886882806122293	2.484862478602308
>500	0.0032579218707481958	0.7449261099984668
>1k	0.004072402338435244	1.840883114187563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1951	0.49436334565652007	No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1916	0.48549470542177986	No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT	1173	0.2972261427242942	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC	1158	0.2934252969094056	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	1060	0.2685931042521329	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG	978	0.24781514713074151	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.20296516651505514	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	654	0.16571687752914616	No Hit
TCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	504	0.12770841938025942	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTG	413	0.10464995476993481	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	408	0.10338300616497191	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.067794419851564E-4	0.0	0.0	0.3109091876578935	0.0
2	5.067794419851564E-4	0.0	0.0	1.5844459253665917	0.0
3	5.067794419851564E-4	0.0	0.0	2.0752618149292155	0.0
4	5.067794419851564E-4	0.0	0.0	2.651976819908324	0.0
5	5.067794419851564E-4	0.0	0.0	4.464726883889228	0.0
6	5.067794419851564E-4	0.0	0.0	5.228950282402844	0.0
7	5.067794419851564E-4	0.0	0.0	6.168772757564317	0.0
8	5.067794419851564E-4	0.0	0.0	7.567737407164341	0.0
9	5.067794419851564E-4	0.0	0.0	8.035748221837633	0.0
10	5.067794419851564E-4	0.0	0.0	9.395944244125793	0.0
11	5.067794419851564E-4	0.0	0.0	11.180061269634535	0.0
12	5.067794419851564E-4	0.0	0.0	12.813664800873688	0.0
13	5.067794419851564E-4	0.0	0.0	13.389112857247833	0.0
14	5.067794419851564E-4	0.0	0.0	13.642249188519418	0.0
15	5.067794419851564E-4	0.0	0.0	14.138386262222888	0.0
16	5.067794419851564E-4	0.0	0.0	15.070860435475575	0.0
17	5.067794419851564E-4	0.0	0.0	16.073523561443206	0.0
18	7.601691629777346E-4	0.0	0.0	17.170447663620077	0.0
19	7.601691629777346E-4	0.0	0.0	17.909585479755428	0.0
20	7.601691629777346E-4	0.0	0.0	18.568398754336133	0.0
21	7.601691629777346E-4	0.0	0.0	19.367083154904737	0.0
22	0.0010135588839703129	0.0	0.0	20.270670899964273	0.0
23	0.0012669486049628911	0.0	0.0	21.123073921383305	0.0
24	0.0012669486049628911	0.0	0.0	21.747426193909018	0.0
25	0.0012669486049628911	0.0	0.0	22.285879351018245	0.0
26	0.0012669486049628911	0.0	0.0	22.802540992122115	0.0
27	0.0012669486049628911	0.0	0.0	23.312614500480173	0.0
28	0.0012669486049628911	0.0	0.0	23.80292361060081	0.0
29	0.0012669486049628911	0.0	0.0	24.34213693687302	0.0
30	0.0012669486049628911	0.0	0.0	24.934055325111682	0.0
31	0.0012669486049628911	0.0	0.0	25.52648049279233	0.0
32	0.0015203383259554692	0.0	0.0	26.06417348073858	0.0
33	0.0015203383259554692	0.0	0.0	26.537252089831725	0.0
34	0.0015203383259554692	0.0	0.0	27.03896373739703	0.0
35	0.0015203383259554692	0.0	0.0	27.563733849572657	0.0
36	0.0015203383259554692	0.0	0.0	28.109028529148688	0.0
37	0.0015203383259554692	0.0	0.0	28.66268506951747	0.0
38	0.0015203383259554692	0.0	0.0	29.177066203132405	0.0
39	0.0015203383259554692	0.0	0.0	29.698035469493146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGTC	70	0.0	45.000004	36
TATTGCG	25	3.8861333E-5	45.000004	1
GCCCCGT	20	7.027651E-4	45.0	16
CCGATTT	20	7.027651E-4	45.0	11
CCTAGTG	20	7.027651E-4	45.0	8
CGACGGT	20	7.027651E-4	45.0	28
ACGTTAT	20	7.027651E-4	45.0	40
GCAACTC	20	7.027651E-4	45.0	38
ACCGATA	40	6.7957444E-9	45.0	10
CACGCAT	20	7.027651E-4	45.0	11
GTTAGAC	20	7.027651E-4	45.0	17
CGATTGA	20	7.027651E-4	45.0	11
TCGATAG	20	7.027651E-4	45.0	1
TACGCAC	20	7.027651E-4	45.0	39
CATAAGA	20	7.027651E-4	45.0	38
CGATAGG	75	0.0	45.0	2
TCCGGTC	20	7.027651E-4	45.0	15
CATATCC	30	2.1618653E-6	44.999996	3
TCGACGG	30	2.1618653E-6	44.999996	2
AAGCTAC	95	0.0	42.63158	8
>>END_MODULE