FastQCFastQC Report
Sat 18 Jun 2016
SRR3550898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794954
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC31350.39436244109722074No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC23830.2997657726107422No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT17450.21950955652779908Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.15007157646857555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC11570.14554301255166965No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10940.13761802569708437No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG10070.1266739962312285No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC9880.12428392083063927No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC7990.10050896026688337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT207.0319715E-445.0000049
GTCGCCC207.0319715E-445.00000421
TGTATCG207.0319715E-445.0000041
ACGTAAG207.0319715E-445.0000041
CGCCCAT207.0319715E-445.00000423
GCCGATA253.889716E-545.09
CATAGCG253.889716E-545.01
TTGCGCA253.889716E-545.01
ACGCGTG253.889716E-545.01
CGTAAGG750.042.02
CCCTCGT1100.040.90909214
TCGTAGG1100.040.9090922
CTACGAA1400.040.1785711
GCGATAA403.4564255E-739.3750049
ACTCGCG403.4564255E-739.3750041
GCGTAAG403.4564255E-739.3750041
ACGATAG750.039.01
CATACGA1100.038.86363618
TAGCACG356.2458603E-638.5714261
CTCGATC356.2458603E-638.57142616