##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794954
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539733116633165	31.0	31.0	33.0	30.0	34.0
2	31.451697834088513	31.0	31.0	34.0	28.0	34.0
3	31.356906185766725	33.0	31.0	34.0	28.0	34.0
4	35.14562855209232	37.0	35.0	37.0	32.0	37.0
5	35.456524528463284	37.0	35.0	37.0	33.0	37.0
6	35.41599136553813	37.0	35.0	37.0	33.0	37.0
7	35.82622516522969	37.0	35.0	37.0	35.0	37.0
8	35.58336582997255	37.0	35.0	37.0	33.0	37.0
9	37.58216701846899	39.0	37.0	39.0	35.0	39.0
10	36.884387021135815	39.0	37.0	39.0	33.0	39.0
11	36.94062801118052	39.0	37.0	39.0	33.0	39.0
12	37.06448046050463	39.0	37.0	39.0	33.0	39.0
13	37.165183142672404	39.0	37.0	39.0	34.0	39.0
14	38.18626738150887	40.0	37.0	41.0	33.0	41.0
15	38.340096408094055	40.0	38.0	41.0	34.0	41.0
16	38.318571892210116	40.0	38.0	41.0	34.0	41.0
17	38.20779063945838	40.0	37.0	41.0	34.0	41.0
18	38.19147774588215	40.0	37.0	41.0	34.0	41.0
19	38.06727433285448	40.0	37.0	41.0	34.0	41.0
20	38.2460607783595	40.0	37.0	41.0	34.0	41.0
21	38.08908314191765	40.0	37.0	41.0	34.0	41.0
22	38.25725891057847	40.0	37.0	41.0	34.0	41.0
23	38.342555669887815	40.0	37.0	41.0	34.0	41.0
24	38.272322675274296	40.0	37.0	41.0	34.0	41.0
25	37.95039084022472	40.0	37.0	41.0	34.0	41.0
26	38.09053228237106	40.0	37.0	41.0	34.0	41.0
27	38.18467106272816	40.0	37.0	41.0	34.0	41.0
28	38.1243405278796	40.0	37.0	41.0	34.0	41.0
29	38.173306883165566	40.0	37.0	41.0	34.0	41.0
30	37.916097032029526	40.0	37.0	41.0	34.0	41.0
31	37.88772683702453	40.0	37.0	41.0	34.0	41.0
32	37.903418310996614	40.0	37.0	41.0	34.0	41.0
33	37.91035959313369	40.0	37.0	41.0	34.0	41.0
34	37.870705726369074	40.0	37.0	41.0	34.0	41.0
35	37.870793781778566	40.0	37.0	41.0	34.0	41.0
36	37.760232667550575	40.0	37.0	41.0	33.0	41.0
37	37.77639964073393	40.0	37.0	41.0	33.0	41.0
38	37.65215345793593	40.0	36.0	41.0	33.0	41.0
39	37.65065777390893	40.0	36.0	41.0	33.0	41.0
40	37.567859272360415	40.0	36.0	41.0	33.0	41.0
41	37.4473453809906	40.0	36.0	41.0	33.0	41.0
42	37.441657001537195	40.0	36.0	41.0	33.0	41.0
43	37.38561224926222	40.0	35.0	41.0	33.0	41.0
44	37.31580192061428	40.0	35.0	41.0	33.0	41.0
45	37.292260935852894	40.0	35.0	41.0	33.0	41.0
46	37.16263708340357	39.0	35.0	41.0	33.0	41.0
47	37.1601010876101	39.0	35.0	41.0	33.0	41.0
48	37.0544270486091	39.0	35.0	41.0	32.0	41.0
49	37.03902993129162	39.0	35.0	41.0	32.0	41.0
50	36.93258226262149	39.0	35.0	41.0	32.0	41.0
51	35.748839555496296	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	20.0
10	24.0
11	19.0
12	15.0
13	14.0
14	24.0
15	38.0
16	73.0
17	82.0
18	140.0
19	283.0
20	434.0
21	752.0
22	1140.0
23	1611.0
24	2352.0
25	3342.0
26	4434.0
27	5425.0
28	6346.0
29	7759.0
30	9632.0
31	12796.0
32	16922.0
33	25461.0
34	49920.0
35	54081.0
36	56957.0
37	91870.0
38	181417.0
39	261518.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.239561031204325	21.46614772678671	25.71821765787706	12.576073584131912
2	31.785612752435988	24.248572873398963	26.639528828083137	17.326285546081913
3	32.017450066293144	23.78716252764311	28.08225381594407	16.11313359011968
4	29.655678190184588	25.641106277847527	26.260513186926538	18.442702345041347
5	26.51172269087268	30.471448662438327	24.679918586484252	18.33691006020474
6	26.874385184551556	34.59030333830636	22.99063845203622	15.544673025105855
7	82.21393942291002	5.689511594381562	7.771141474852633	4.3254075078558
8	83.53804119483644	5.0396626723055675	6.872850504557497	4.549445628300505
9	77.29151623867546	8.162484873338585	8.939636758856487	5.606362129129485
10	38.884639865954505	33.9512726522541	15.27472029828141	11.88936718350999
11	27.506623024728476	24.850242907136767	28.690339315230815	18.95279475290394
12	27.50888730668693	22.30066142191875	30.67007650757151	19.52037476382281
13	24.251969296336643	23.937108310669547	31.465720029083442	20.345202363910364
14	19.659376517383397	28.247043225142587	29.858205632023992	22.235374625450028
15	19.347157194001163	28.295725287249322	32.931465216855315	19.425652301894196
16	23.346633893281876	26.643428424789363	30.343013558017194	19.66692412391157
17	21.87334109898183	27.575935211345566	28.59272360413307	21.958000085539542
18	22.607471627289126	28.101374419148783	29.2610641622031	20.030089791358996
19	23.19127899224358	28.72153608888062	27.754184518852664	20.333000400023145
20	25.409646344316776	28.18829768766495	28.733738052767833	17.66831791525044
21	24.489215728205657	27.607635158763905	28.72468092493402	19.178468188096417
22	22.52696382432191	26.2746020524458	28.798144295141604	22.400289828090685
23	22.040520583580935	26.998165931613656	29.629890534546654	21.33142295025876
24	22.935037750611986	25.95055814550276	29.79618946505081	21.318214638834448
25	22.156753724114857	28.104519255202188	27.21226637012959	22.526460650553364
26	20.689247428153077	28.61322793520128	28.93525914707015	21.762265489575498
27	22.283553513788217	28.077725252027165	28.745562636328643	20.893158597855976
28	19.202494735544445	28.442274647338085	30.86140833306078	21.493822284056687
29	21.44275014654936	26.285042908143115	30.38943133816548	21.88277560714205
30	22.982084497970952	25.511664825889298	29.485479662974214	22.02077101316554
31	23.549035541679142	27.357683589239123	27.771544013867466	21.32173685521426
32	23.88276554366668	27.170251360456078	28.396989008169026	20.54999408770822
33	23.05844111734767	27.734812328763674	27.177924760426393	22.028821793462264
34	21.359474887855146	25.77130249045857	31.44446093736241	21.424761684323872
35	22.127066471770693	25.919235578410827	29.057530372826605	22.89616757699188
36	22.231097648417393	28.534732827308247	28.268176523421477	20.96599300085288
37	23.59318903986897	27.938975085350847	28.95777617321254	19.510059701567638
38	21.293433330733603	27.602351834194177	28.643544154756125	22.460670680316095
39	22.553506240612663	26.09056624660043	28.510454692975944	22.845472819810958
40	23.59910133164938	24.457893161113724	29.581082678997777	22.361922828239116
41	21.982529806756116	26.816394407726733	28.298492742976322	22.902583042540826
42	22.71024486951446	26.884448659922462	28.408436211403426	21.99687025915965
43	22.727101190760724	25.70903473660111	29.675805140926393	21.88805893171177
44	21.479985005421696	25.429395914732172	29.527243085763455	23.563375994082676
45	22.44129849022711	24.12076673618851	28.966833301046353	24.471101472538034
46	22.90686001957346	25.591040487877287	28.88821239971118	22.613887092838077
47	21.572443185391858	25.251020813782937	31.244323570923598	21.932212429901604
48	20.92875814198054	24.700171330668187	30.46981334769056	23.90125717966071
49	21.52917024129698	24.63438136043092	31.667870090596438	22.168578307675666
50	20.96284816479947	24.301280325654062	29.984628041370947	24.751243468175517
51	20.492506484651944	23.647909187198252	29.04985697285629	26.80972735529351
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	373.0
1	813.0
2	1253.0
3	1995.0
4	2737.0
5	2016.0
6	1295.0
7	1219.0
8	1143.0
9	1171.0
10	1199.0
11	1219.0
12	1239.0
13	1308.5
14	1378.0
15	1385.0
16	1392.0
17	1461.0
18	1530.0
19	1725.5
20	1921.0
21	2207.5
22	2494.0
23	2941.0
24	3388.0
25	4187.0
26	6522.0
27	8058.0
28	10000.0
29	11942.0
30	14805.5
31	17669.0
32	19178.5
33	20688.0
34	24384.5
35	28081.0
36	29210.0
37	30339.0
38	32841.5
39	35344.0
40	38738.5
41	42133.0
42	45485.5
43	48838.0
44	52738.0
45	56638.0
46	62725.5
47	68813.0
48	73697.5
49	78582.0
50	76684.5
51	74787.0
52	64275.0
53	53763.0
54	47433.5
55	41104.0
56	37540.0
57	33976.0
58	32380.0
59	30784.0
60	29605.0
61	28426.0
62	24924.5
63	21423.0
64	18069.0
65	14715.0
66	11693.0
67	8671.0
68	7130.0
69	5589.0
70	4386.5
71	3184.0
72	2853.5
73	2523.0
74	1856.0
75	996.0
76	803.0
77	595.0
78	387.0
79	224.0
80	61.0
81	65.5
82	70.0
83	41.0
84	12.0
85	13.0
86	14.0
87	13.5
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.82586223280411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94213042475393	19.740259477008237
2	11.173614021820164	6.218308888273922
3	3.8902662247457447	3.247500360561182
4	1.8944321662008285	2.1085683386438765
5	1.166934696824391	1.6235482054257269
6	0.8122568685089048	1.35610486324666
7	0.6192323822247696	1.2061472470514276
8	0.5119085195011502	1.139543675155018
9	0.4356539082305743	1.0910201068445984
>10	8.277055931659588	54.17652485393732
>50	0.22574491486207046	3.8044983455246517
>100	0.04442369808440337	2.184540375943271
>500	0.0031731212917430977	0.6258842511363536
>1k	0.0031731212917430977	1.4775510112477321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	3135	0.39436244109722074	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	2383	0.2997657726107422	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	1745	0.21950955652779908	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.15007157646857555	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	1157	0.14554301255166965	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1094	0.13761802569708437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	1007	0.1266739962312285	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	988	0.12428392083063927	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	799	0.10050896026688337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2579344213627455E-4	0.0	0.0	0.22856668436161087	0.0
2	1.2579344213627455E-4	0.0	0.0	1.149123093914868	0.0
3	1.2579344213627455E-4	0.0	0.0	1.4774439778905446	0.0
4	1.2579344213627455E-4	0.0	0.0	1.9002357369105634	0.0
5	1.2579344213627455E-4	0.0	0.0	3.0847067880657244	0.0
6	1.2579344213627455E-4	0.0	0.0	3.6898990381833414	0.0
7	1.2579344213627455E-4	0.0	0.0	4.391197478093072	0.0
8	1.2579344213627455E-4	0.0	0.0	5.459812769040725	0.0
9	1.2579344213627455E-4	0.0	0.0	5.819078839781924	0.0
10	1.2579344213627455E-4	0.0	0.0	6.750453485358901	0.0
11	1.2579344213627455E-4	0.0	0.0	8.068894552389194	0.0
12	1.2579344213627455E-4	0.0	0.0	9.24305054128918	0.0
13	1.2579344213627455E-4	0.0	0.0	9.638041949597083	0.0
14	2.515868842725491E-4	0.0	0.0	9.800063903068605	0.0
15	2.515868842725491E-4	0.0	0.0	10.133039144403323	0.0
16	3.7738032640882366E-4	0.0	0.0	10.772195623897735	0.0
17	3.7738032640882366E-4	0.0	0.0	11.588091889593612	0.0
18	3.7738032640882366E-4	0.0	0.0	12.407761958553577	0.0
19	3.7738032640882366E-4	0.0	0.0	13.012451034902648	0.0
20	3.7738032640882366E-4	0.0	0.0	13.571099711429843	0.0
21	3.7738032640882366E-4	0.0	0.0	14.207740322081529	0.0
22	3.7738032640882366E-4	0.0	0.0	14.973319210922897	0.0
23	3.7738032640882366E-4	0.0	0.0	15.640905008340106	0.0
24	3.7738032640882366E-4	0.0	0.0	16.186345373442993	0.0
25	3.7738032640882366E-4	0.0	0.0	16.64423350281903	0.0
26	5.031737685450982E-4	0.0	0.0	17.067780022491867	0.0
27	5.031737685450982E-4	0.0	0.0	17.51006976504301	0.0
28	5.031737685450982E-4	0.0	0.0	17.979530891095585	0.0
29	5.031737685450982E-4	0.0	0.0	18.461948741688197	0.0
30	5.031737685450982E-4	0.0	0.0	18.984872080648692	0.0
31	5.031737685450982E-4	0.0	0.0	19.503644236018687	0.0
32	5.031737685450982E-4	0.0	0.0	19.975872817798262	0.0
33	5.031737685450982E-4	0.0	0.0	20.44696925859861	0.0
34	7.547606528176473E-4	0.0	0.0	20.916556178093323	0.0
35	7.547606528176473E-4	0.0	0.0	21.41369186141588	0.0
36	7.547606528176473E-4	0.0	0.0	21.902022003788897	0.0
37	7.547606528176473E-4	0.0	0.0	22.398528719900774	0.0
38	7.547606528176473E-4	0.0	0.0	22.886355688505247	0.0
39	7.547606528176473E-4	0.0	0.0	23.39682547669425	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.0319715E-4	45.000004	9
GTCGCCC	20	7.0319715E-4	45.000004	21
TGTATCG	20	7.0319715E-4	45.000004	1
ACGTAAG	20	7.0319715E-4	45.000004	1
CGCCCAT	20	7.0319715E-4	45.000004	23
GCCGATA	25	3.889716E-5	45.0	9
CATAGCG	25	3.889716E-5	45.0	1
TTGCGCA	25	3.889716E-5	45.0	1
ACGCGTG	25	3.889716E-5	45.0	1
CGTAAGG	75	0.0	42.0	2
CCCTCGT	110	0.0	40.909092	14
TCGTAGG	110	0.0	40.909092	2
CTACGAA	140	0.0	40.17857	11
GCGATAA	40	3.4564255E-7	39.375004	9
ACTCGCG	40	3.4564255E-7	39.375004	1
GCGTAAG	40	3.4564255E-7	39.375004	1
ACGATAG	75	0.0	39.0	1
CATACGA	110	0.0	38.863636	18
TAGCACG	35	6.2458603E-6	38.571426	1
CTCGATC	35	6.2458603E-6	38.571426	16
>>END_MODULE