SRR3550894_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713995
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3710	0.5196114818731223	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2857	0.4001428581432643	Illumina PCR Primer Index 7 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2780	0.389358468896841	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	2196	0.30756517902786434	Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1490	0.20868493476845074	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1489	0.20854487776525046	Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1297	0.18165393315079237	Illumina PCR Primer Index 7 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1249	0.17493119699717785	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	1128	0.15798429960994126	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	930	0.13025301297628133	No Hit
ACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	852	0.11932856672665776	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	717	0.10042087129461691	Illumina PCR Primer Index 7 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	25	3.889315E-5	45.000004	2
ACGCCCG	20	7.0314883E-4	45.000004	22
CAAGTCG	25	3.889315E-5	45.000004	1
TTCAGCG	25	3.889315E-5	45.000004	1
ACTTGCG	25	3.889315E-5	45.000004	1
CTATCCG	20	7.0314883E-4	45.000004	11
GCACGAC	25	3.889315E-5	45.000004	25
ATGTCGA	20	7.0314883E-4	45.000004	28
TGTCGAA	20	7.0314883E-4	45.000004	29
CGCCCGG	20	7.0314883E-4	45.000004	23
TATCCGT	20	7.0314883E-4	45.000004	12
GCGAGTG	20	7.0314883E-4	45.000004	1
AGGTCGA	20	7.0314883E-4	45.000004	42
TTAGGCG	35	1.211065E-7	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
TATAGCG	55	1.8189894E-12	45.0	1
CGGTCTA	55	1.8189894E-12	45.0	31
TCGAGCG	30	2.1643427E-6	44.999996	1
TTGCACG	30	2.1643427E-6	44.999996	1
AGTAACG	60	0.0	44.999996	1