##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713995
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40179132907093	31.0	31.0	33.0	28.0	34.0
2	31.319150694332592	31.0	31.0	34.0	28.0	34.0
3	31.214318027437166	31.0	31.0	34.0	28.0	34.0
4	35.0148404400591	35.0	35.0	37.0	32.0	37.0
5	35.35759914285114	35.0	35.0	37.0	33.0	37.0
6	35.34628673870265	37.0	35.0	37.0	33.0	37.0
7	35.74577833178103	37.0	35.0	37.0	35.0	37.0
8	35.59048872891267	37.0	35.0	37.0	33.0	37.0
9	37.53355975882184	39.0	37.0	39.0	35.0	39.0
10	36.8188796840314	39.0	37.0	39.0	32.0	39.0
11	36.925033088467	39.0	37.0	39.0	33.0	39.0
12	37.11241395247866	39.0	37.0	39.0	34.0	39.0
13	37.25896119720726	39.0	37.0	39.0	34.0	39.0
14	38.26346963214028	40.0	38.0	41.0	34.0	41.0
15	38.42208278769459	40.0	38.0	41.0	34.0	41.0
16	38.38168894740159	40.0	38.0	41.0	34.0	41.0
17	38.2978816378266	40.0	38.0	41.0	34.0	41.0
18	38.267123719353776	40.0	38.0	41.0	34.0	41.0
19	38.101517517629674	40.0	37.0	41.0	34.0	41.0
20	38.26040938662035	40.0	37.0	41.0	34.0	41.0
21	38.1246913493792	40.0	37.0	41.0	34.0	41.0
22	38.30682007577084	40.0	37.0	41.0	34.0	41.0
23	38.38284861938809	40.0	38.0	41.0	34.0	41.0
24	38.33052892527259	40.0	37.0	41.0	34.0	41.0
25	37.982620326472876	40.0	37.0	41.0	34.0	41.0
26	38.12180617511327	40.0	37.0	41.0	34.0	41.0
27	38.1982758982906	40.0	37.0	41.0	34.0	41.0
28	38.1203019628989	40.0	37.0	41.0	34.0	41.0
29	38.1555753191549	40.0	37.0	41.0	34.0	41.0
30	37.89298944670481	40.0	37.0	41.0	33.0	41.0
31	37.78960917093257	40.0	37.0	41.0	33.0	41.0
32	37.77701244406473	40.0	37.0	41.0	33.0	41.0
33	37.76983732379078	40.0	37.0	41.0	33.0	41.0
34	37.71686356347033	40.0	37.0	41.0	33.0	41.0
35	37.70547552854011	40.0	37.0	41.0	33.0	41.0
36	37.58236962443715	40.0	37.0	41.0	33.0	41.0
37	37.56290030042227	40.0	37.0	41.0	33.0	41.0
38	37.42936855300107	40.0	37.0	41.0	33.0	41.0
39	37.42218642987696	40.0	37.0	41.0	33.0	41.0
40	37.32154987079741	40.0	36.0	41.0	33.0	41.0
41	37.21783065707743	40.0	36.0	41.0	32.0	41.0
42	37.23386998508393	40.0	36.0	41.0	32.0	41.0
43	37.16583169349926	40.0	36.0	41.0	32.0	41.0
44	37.08741097626734	40.0	36.0	41.0	32.0	41.0
45	37.03480416529528	39.0	36.0	41.0	32.0	41.0
46	36.8880132213811	39.0	35.0	41.0	31.0	41.0
47	36.892350786770216	39.0	35.0	41.0	31.0	41.0
48	36.793086786322036	39.0	35.0	41.0	31.0	41.0
49	36.80808689136479	39.0	35.0	41.0	31.0	41.0
50	36.691200918773944	39.0	35.0	41.0	31.0	41.0
51	35.381574100658966	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	19.0
10	13.0
11	16.0
12	14.0
13	11.0
14	11.0
15	24.0
16	43.0
17	80.0
18	121.0
19	248.0
20	417.0
21	684.0
22	1119.0
23	1724.0
24	2576.0
25	3990.0
26	5160.0
27	5894.0
28	6604.0
29	7472.0
30	9260.0
31	11808.0
32	16088.0
33	22053.0
34	37851.0
35	46256.0
36	54468.0
37	87854.0
38	174109.0
39	217978.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.04144286724697	22.51318286542623	26.69360429694886	12.751769970377943
2	32.02739514982598	24.382103516131064	27.05523147921204	16.53526985483092
3	31.004838969460568	23.88280029972199	29.177375191703025	15.934985539114418
4	27.486887163075373	26.62973830348952	27.93436928830034	17.94900524513477
5	24.71641958277019	30.098109930741813	26.59290331164784	18.59256717484016
6	25.322166121611495	34.31620669612532	24.837148719528848	15.524478462734335
7	81.37788079748458	4.61557854046597	9.392642805621888	4.613897856427566
8	82.45982114720691	3.9141730684388545	8.486894165925532	5.1391116184287
9	76.2980132914096	7.2113950377803775	10.804277340877738	5.686314329932282
10	33.231185092332574	39.96260478014552	16.02742316122662	10.778786966295282
11	22.142171863948626	24.306892905412504	33.8923942044412	19.65854102619766
12	21.859676888493617	21.998333321661917	35.94072787624563	20.201261913598834
13	21.543288118264133	23.88097955868038	36.19072962695817	18.38500269609731
14	18.018193404715717	28.558323237557687	32.798549009446845	20.62493434827975
15	17.020987541929568	27.659717505024545	36.29101044124959	19.0282845117963
16	19.247333664801573	27.619661202109256	32.63398203068649	20.499023102402678
17	19.795656832330756	26.691643498904057	30.881028578631504	22.631671090133683
18	19.624087003410388	26.531978515255712	33.035665515864956	20.808268965468947
19	19.595095203747924	27.696972667875823	31.002317943402964	21.705614184973285
20	21.484744290926407	28.56994796882331	30.731307642210382	19.2140000980399
21	21.378581082500578	28.25495976862583	31.418567356914263	18.94789179195933
22	19.830671083130834	25.319505038550687	30.294189735222236	24.55563414309624
23	18.015952492664514	28.12078515955994	30.905398497188358	22.95786385058719
24	19.674367467559296	26.21811077108383	31.746720915412574	22.360800845944297
25	18.873381466256767	29.308048375688905	28.873311437755167	22.94525872029916
26	19.085427769102026	29.752869417853066	28.95930643772015	22.202396375324756
27	21.2495885825531	28.527790810860022	29.49390401893571	20.728716587651174
28	17.686258307131002	29.488721909817293	30.775845769228077	22.049174013823627
29	20.794683436158518	26.182396235267753	29.54726573715502	23.475654591418706
30	22.715285121044264	26.773016617763428	28.82050994754865	21.691188313643654
31	21.705614184973285	30.151471648961127	25.980994264665718	22.16191990139987
32	22.368784095126717	30.006652707652016	26.55200666671335	21.072556530507917
33	21.60099160358266	28.135631201899177	25.793177823374116	24.470199371144055
34	19.52800789921498	29.13395752071093	27.626383938262872	23.711650641811215
35	20.4633085665866	27.519380387817844	27.69963375093663	24.317677294658928
36	21.45967408735355	28.816308237452642	26.775257529814635	22.94876014537917
37	21.053088607063074	29.647826665452836	28.93101492307369	20.368069804410396
38	20.10098109930742	28.412804011232573	28.03647084363336	23.44974404582665
39	21.234462426207468	26.088558043123548	27.149769956372243	25.527209574296737
40	20.933059755320414	26.00802526628338	30.12990287046828	22.929012107927925
41	19.092010448252438	27.57498301808836	28.373447993333283	24.95955854032591
42	21.018074356263	26.737442138950552	29.102444694990858	23.142038809795586
43	22.114020406305364	25.733093369001182	28.49935923921036	23.653526985483094
44	21.54636937233454	26.404946813353035	27.37204042045112	24.6766433938613
45	21.696090308755664	25.194013963683222	27.493189728219384	25.616705999341733
46	23.131254420549162	26.21685025805503	27.751174728114343	22.900720593281466
47	19.43613050511558	26.49388301038523	30.66828199077024	23.401704493728946
48	19.846357467489266	25.298776602077044	29.56617343258706	25.288692497846622
49	21.395808093894214	23.49554268587315	30.870804417397878	24.237844802834754
50	20.492720537258663	23.31346858171276	30.112955973081046	26.080854907947536
51	19.343832940006582	22.872569135638205	28.36574485815727	29.417853066197942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	347.0
1	516.5
2	686.0
3	3133.0
4	5580.0
5	3648.5
6	1717.0
7	1693.0
8	1669.0
9	1776.5
10	1884.0
11	1912.5
12	1941.0
13	1959.5
14	1978.0
15	1947.5
16	1917.0
17	1976.0
18	2035.0
19	2185.0
20	2335.0
21	2626.5
22	2918.0
23	3062.5
24	3207.0
25	3941.5
26	5913.0
27	7150.0
28	8098.0
29	9046.0
30	10587.5
31	12129.0
32	14187.5
33	16246.0
34	19189.0
35	22132.0
36	24068.0
37	26004.0
38	28572.5
39	31141.0
40	33731.0
41	36321.0
42	40299.0
43	44277.0
44	50393.5
45	56510.0
46	67824.5
47	79139.0
48	83950.5
49	88762.0
50	83824.5
51	78887.0
52	65631.0
53	52375.0
54	44018.5
55	35662.0
56	29963.0
57	24264.0
58	21642.5
59	19021.0
60	16922.5
61	14824.0
62	12599.0
63	10374.0
64	8561.0
65	6748.0
66	5287.5
67	3827.0
68	3140.0
69	2453.0
70	1950.0
71	1447.0
72	1263.0
73	1079.0
74	836.5
75	443.5
76	293.0
77	271.5
78	250.0
79	151.0
80	52.0
81	65.5
82	79.0
83	44.0
84	9.0
85	6.5
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.127140725267324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37360580113688	25.07150695046291
2	11.842429012489903	8.319813409014428
3	4.396826708112251	4.633438515614187
4	2.328409515611476	3.2716147488374343
5	1.3862575364664649	2.4347631782459955
6	1.002788854685399	2.113506312975819
7	0.7583481752200442	1.8647022148792918
8	0.5847053309591155	1.6431221154731883
9	0.5170653522183151	1.6346724652379403
>10	5.647859677338429	40.64764021290969
>50	0.11607467942782364	2.6666010211007816
>100	0.03882497863500502	2.367968684712781
>500	0.0032020600936086612	0.7723104135570186
>1k	0.003602317605309744	2.5583397569786106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3710	0.5196114818731223	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2857	0.4001428581432643	Illumina PCR Primer Index 7 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2780	0.389358468896841	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	2196	0.30756517902786434	Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1490	0.20868493476845074	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1489	0.20854487776525046	Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1297	0.18165393315079237	Illumina PCR Primer Index 7 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1249	0.17493119699717785	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	1128	0.15798429960994126	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	930	0.13025301297628133	No Hit
ACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	852	0.11932856672665776	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	717	0.10042087129461691	Illumina PCR Primer Index 7 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8011400640060507E-4	0.0	0.0	0.32451207641510094	0.0
2	2.8011400640060507E-4	0.0	0.0	1.4778814977695922	0.0
3	2.8011400640060507E-4	0.0	0.0	2.023543582237971	0.0
4	2.8011400640060507E-4	0.0	0.0	2.753660739921148	0.0
5	2.8011400640060507E-4	0.0	0.0	4.66207746552847	0.0
6	2.8011400640060507E-4	0.0	0.0	5.816567342908564	0.0
7	2.8011400640060507E-4	0.0	0.0	7.070497692560872	0.0
8	2.8011400640060507E-4	0.0	0.0	9.06490941813318	0.0
9	2.8011400640060507E-4	0.0	0.0	9.803710110014777	0.0
10	2.8011400640060507E-4	0.0	0.0	11.381312194062984	0.0
11	2.8011400640060507E-4	0.0	0.0	13.545613064517259	0.0
12	2.8011400640060507E-4	0.0	0.0	15.54632735523358	0.0
13	2.8011400640060507E-4	0.0	0.0	16.256416361459113	0.0
14	2.8011400640060507E-4	0.0	0.0	16.537650823885322	0.0
15	2.8011400640060507E-4	0.0	0.0	16.99549716734711	0.0
16	2.8011400640060507E-4	0.0	0.0	17.849424715859353	0.0
17	2.8011400640060507E-4	0.0	0.0	19.133467321199728	0.0
18	2.8011400640060507E-4	0.0	0.0	20.457006001442586	0.0
19	2.8011400640060507E-4	0.0	0.0	21.345527629745305	0.0
20	2.8011400640060507E-4	0.0	0.0	22.141331521929427	0.0
21	2.8011400640060507E-4	0.0	0.0	23.030273321241744	0.0
22	4.201710096009076E-4	0.0	0.0	24.112493784970482	0.0
23	4.201710096009076E-4	0.0	0.0	25.089531439295794	0.0
24	4.201710096009076E-4	0.0	0.0	25.84289805951022	0.0
25	4.201710096009076E-4	0.0	0.0	26.467132122773968	0.0
26	4.201710096009076E-4	0.0	0.0	27.090525844018515	0.0
27	4.201710096009076E-4	0.0	0.0	27.7301661776343	0.0
28	4.201710096009076E-4	0.0	0.0	28.366165029166872	0.0
29	4.201710096009076E-4	0.0	0.0	29.068130729206786	0.0
30	4.201710096009076E-4	0.0	0.0	29.89208607903417	0.0
31	4.201710096009076E-4	0.0	0.0	30.59853360317649	0.0
32	4.201710096009076E-4	0.0	0.0	31.23131114363546	0.0
33	4.201710096009076E-4	0.0	0.0	31.83803808149917	0.0
34	4.201710096009076E-4	0.0	0.0	32.464232942807726	0.0
35	4.201710096009076E-4	0.0	0.0	33.1488315744508	0.0
36	5.602280128012101E-4	0.0	0.0	33.80289777939621	0.0
37	5.602280128012101E-4	0.0	0.0	34.471389855671255	0.0
38	5.602280128012101E-4	0.0	0.0	35.096324203951006	0.0
39	5.602280128012101E-4	0.0	0.0	35.76201514016205	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	25	3.889315E-5	45.000004	2
ACGCCCG	20	7.0314883E-4	45.000004	22
CAAGTCG	25	3.889315E-5	45.000004	1
TTCAGCG	25	3.889315E-5	45.000004	1
ACTTGCG	25	3.889315E-5	45.000004	1
CTATCCG	20	7.0314883E-4	45.000004	11
GCACGAC	25	3.889315E-5	45.000004	25
ATGTCGA	20	7.0314883E-4	45.000004	28
TGTCGAA	20	7.0314883E-4	45.000004	29
CGCCCGG	20	7.0314883E-4	45.000004	23
TATCCGT	20	7.0314883E-4	45.000004	12
GCGAGTG	20	7.0314883E-4	45.000004	1
AGGTCGA	20	7.0314883E-4	45.000004	42
TTAGGCG	35	1.211065E-7	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
TATAGCG	55	1.8189894E-12	45.0	1
CGGTCTA	55	1.8189894E-12	45.0	31
TCGAGCG	30	2.1643427E-6	44.999996	1
TTGCACG	30	2.1643427E-6	44.999996	1
AGTAACG	60	0.0	44.999996	1
>>END_MODULE