##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537693
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16160150866759	33.0	31.0	34.0	30.0	34.0
2	32.264024266635424	34.0	31.0	34.0	30.0	34.0
3	32.058845846979594	33.0	31.0	34.0	30.0	34.0
4	35.8560033327568	37.0	35.0	37.0	35.0	37.0
5	33.78210428627488	37.0	35.0	37.0	32.0	37.0
6	34.78433232346339	37.0	35.0	37.0	32.0	37.0
7	35.85380133273076	37.0	35.0	37.0	33.0	37.0
8	35.899636037664614	37.0	35.0	37.0	35.0	37.0
9	37.89061788046339	39.0	38.0	39.0	35.0	39.0
10	37.44966737524945	39.0	37.0	39.0	34.0	39.0
11	37.43423663689131	39.0	37.0	39.0	35.0	39.0
12	37.43005953211219	39.0	37.0	39.0	35.0	39.0
13	37.446924174203495	39.0	37.0	39.0	35.0	39.0
14	38.69486677341903	40.0	38.0	41.0	35.0	41.0
15	38.82264600803804	40.0	38.0	41.0	35.0	41.0
16	38.75104567104277	40.0	38.0	41.0	35.0	41.0
17	38.729525956261284	40.0	38.0	41.0	35.0	41.0
18	38.413884874826344	39.0	38.0	41.0	35.0	41.0
19	38.034767051086774	39.0	37.0	41.0	34.0	41.0
20	37.776921031146024	39.0	35.0	41.0	34.0	41.0
21	37.63807228288261	39.0	35.0	41.0	34.0	41.0
22	37.64640417487302	39.0	35.0	41.0	34.0	41.0
23	37.650216759377564	39.0	35.0	41.0	34.0	41.0
24	37.55283033255036	39.0	35.0	41.0	34.0	41.0
25	37.35524918494382	39.0	35.0	41.0	33.0	41.0
26	37.400057281757434	39.0	35.0	41.0	33.0	41.0
27	37.378781200424775	39.0	35.0	41.0	33.0	41.0
28	37.2488260029422	39.0	35.0	41.0	33.0	41.0
29	37.16212783130894	39.0	35.0	41.0	33.0	41.0
30	36.85687743749686	39.0	35.0	41.0	33.0	41.0
31	36.570079952686754	39.0	35.0	41.0	32.0	41.0
32	36.086233222303434	39.0	35.0	41.0	30.0	41.0
33	35.462291679452775	39.0	35.0	41.0	23.0	41.0
34	34.90361414413057	39.0	35.0	41.0	18.0	41.0
35	34.528755256252175	39.0	35.0	41.0	16.0	41.0
36	34.284186329373824	39.0	35.0	41.0	15.0	41.0
37	34.154705380207666	39.0	34.0	41.0	14.0	41.0
38	34.03143243449329	39.0	34.0	41.0	12.0	41.0
39	33.92858564273666	39.0	34.0	41.0	10.0	41.0
40	33.831812949024815	38.0	34.0	41.0	10.0	41.0
41	33.70130167214377	38.0	34.0	41.0	10.0	41.0
42	33.623872730349845	38.0	33.0	41.0	10.0	41.0
43	33.55550100150086	38.0	33.0	41.0	10.0	41.0
44	33.39625399623949	38.0	33.0	41.0	10.0	41.0
45	33.348036890939625	38.0	33.0	41.0	10.0	41.0
46	33.234682244329015	38.0	33.0	40.0	10.0	41.0
47	33.21807611406509	38.0	33.0	40.0	10.0	41.0
48	33.12634719068316	38.0	33.0	40.0	10.0	41.0
49	33.029823709812106	37.0	33.0	40.0	10.0	41.0
50	32.939480335433046	37.0	33.0	40.0	10.0	41.0
51	32.083480722270885	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	3.0
10	21.0
11	17.0
12	14.0
13	11.0
14	18.0
15	33.0
16	52.0
17	117.0
18	238.0
19	501.0
20	826.0
21	1280.0
22	2016.0
23	3426.0
24	5949.0
25	10524.0
26	16056.0
27	18116.0
28	16064.0
29	13283.0
30	11699.0
31	11834.0
32	12915.0
33	16370.0
34	27564.0
35	37795.0
36	35283.0
37	48506.0
38	85435.0
39	161596.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.159628263711824	17.980706462609707	21.184021365351605	27.67564390832687
2	44.24606606372038	20.46911527581724	21.544264106097717	13.740554554364664
3	26.485745583446317	19.508901919868773	40.39052024110412	13.614832255580787
4	24.220698428285285	21.158542142077355	39.73178003061226	14.888979399025093
5	19.541820332420173	30.535826205660104	36.357921713691645	13.564431748228078
6	22.333190128939748	29.428502881755946	37.308092164115955	10.93021482518835
7	67.039370049452	5.640393309937083	24.091256534862833	3.2289801057480756
8	68.96147057893631	5.020708843150273	23.332831188057128	2.6849893898562938
9	63.761663253938586	7.417243668784976	24.88222833475608	3.93886474252036
10	33.092861539949375	23.58167206937788	32.34317724054432	10.982289150128418
11	25.06597630990175	22.572174084468273	37.46319926054459	14.898650345085393
12	23.430842506783613	20.051590777637056	40.52126399265008	15.996302722929256
13	20.592419838086045	21.706624412071573	40.98081991024618	16.7201358395962
14	16.606688203119624	24.09795180521227	41.28657058953715	18.008789402130958
15	17.77147926418979	23.482358892527895	43.265208957527804	15.48095288575451
16	19.979430641648673	22.31552205440651	42.44410844106209	15.260938862882723
17	19.28647016048191	23.618496056299783	40.59602784488546	16.49900593833284
18	19.19255039585786	23.549311596022264	41.45711400371587	15.801024004403999
19	20.22920142162907	24.3066210644364	39.45467023003833	16.009507283896202
20	20.603764601733705	23.874032207969975	41.19060504786189	14.331598142434437
21	19.988171689049327	23.908624438108735	40.78777294850407	15.315430924337866
22	18.96788687968785	22.76150145157181	40.944925821983915	17.325685846756418
23	18.595927415830225	23.249512268153016	41.650904884385696	16.503655431631064
24	17.929190076865424	23.550613454145765	42.32136181798908	16.198834650999732
25	17.98331017885671	25.273157731270445	39.88298155267039	16.860550537202457
26	17.34632959700052	25.658135776363096	39.75260976058829	17.2429248660481
27	17.8218797715425	24.576849614928964	41.003509437541496	16.597761175987042
28	16.975114052070605	25.119910432161102	41.64737126947905	16.25760424628924
29	17.91282386045569	24.082329507730247	40.421764836068164	17.5830817957459
30	17.76311017625299	24.657006879390284	40.699804535301745	16.88007840905498
31	18.726299207912323	24.89580485518688	39.02728880606591	17.35060713083488
32	18.997271677332606	25.237263643008184	38.619807213409885	17.14565746624933
33	19.23365191661413	26.286189331086696	37.08156885062665	17.398589901672516
34	17.960062712365605	26.718406228089265	36.947663443637914	18.37386761590722
35	18.821706810391802	26.899736466719858	35.50204298735524	18.7765137355331
36	19.065340259218548	28.678260643155106	33.79195935226979	18.46443974535655
37	19.11071931380918	29.15883227045917	34.79271628977874	16.937732125952916
38	18.766842789472804	29.087044093934644	33.984076415352256	18.1620367012403
39	19.552421177140115	27.732553706297086	33.954691617707496	18.760333498855296
40	19.631276583477934	27.193026503971595	34.79476206682996	18.380934845720514
41	17.953181462284242	27.917789519298186	34.2470517562996	19.881977262117974
42	18.322537209894865	27.932667897852493	33.24573687959486	20.49905801265778
43	19.344867796307557	27.151553023751475	33.68762472265773	19.815954457283244
44	19.514481311826636	27.24138123427309	32.77316238076374	20.47097507313653
45	19.118344482818262	27.85437043071046	33.14158822971473	19.885696856756553
46	18.06439734197767	29.108803722570315	33.229928602380916	19.596870333071102
47	18.79269397221091	26.99867768410599	34.89649298019502	19.312135363488085
48	18.829331979400884	26.305159263743437	35.147751598030844	19.71775715882483
49	18.70007606571036	27.320050660878977	34.62328875399159	19.356584519419073
50	17.97475511118798	27.16233984820334	34.84274483766759	20.020160202941085
51	18.3498762304884	26.15228392409795	34.17042810674493	21.32741173866872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	568.0
1	1137.5
2	1707.0
3	17553.0
4	33399.0
5	22085.5
6	10772.0
7	10135.5
8	9499.0
9	9273.0
10	9047.0
11	8817.0
12	8587.0
13	8227.5
14	7868.0
15	7444.5
16	7021.0
17	6526.0
18	6031.0
19	5708.0
20	5385.0
21	5130.5
22	4876.0
23	4956.0
24	5036.0
25	5115.0
26	6214.0
27	7234.0
28	8350.0
29	9466.0
30	10778.0
31	12090.0
32	13319.5
33	14549.0
34	16310.5
35	18072.0
36	18855.5
37	19639.0
38	21334.5
39	23030.0
40	24458.5
41	25887.0
42	26802.0
43	27717.0
44	29422.0
45	31127.0
46	32389.0
47	33651.0
48	34206.0
49	34761.0
50	33407.5
51	32054.0
52	29105.0
53	26156.0
54	23804.0
55	21452.0
56	20078.0
57	18704.0
58	17393.0
59	16082.0
60	15285.5
61	14489.0
62	13276.5
63	12064.0
64	10391.0
65	8718.0
66	7185.0
67	5652.0
68	4791.5
69	3931.0
70	3173.5
71	2416.0
72	2026.0
73	1636.0
74	1293.0
75	735.0
76	520.0
77	423.0
78	326.0
79	243.5
80	161.0
81	123.0
82	85.0
83	70.5
84	56.0
85	34.0
86	12.0
87	10.0
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.75554597084501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46242584897006	28.104182083341467
2	7.94113008178784	5.8376114356322635
3	3.238128060546756	3.5705749436672853
4	1.9506120007732255	2.86783236262809
5	1.3708702011672504	2.5193541349532214
6	1.0738534461352482	2.3682041823224678
7	0.8729227779082885	2.2459327304684082
8	0.7459992492911308	2.1935687761228824
9	0.6433720595664565	2.1282742180576917
>10	5.5517036016273575	38.77874228043314
>50	0.11135564920679455	2.517997689965471
>100	0.03457618331533259	2.5488348916895305
>500	0.0020338931361960346	0.5999237426545969
>1k	5.084732840490087E-4	0.21417838289164112
>5k	0.0	0.0
>10k+	5.084732840490087E-4	3.5047881451718554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18753	3.487677912861056	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.2131327727904213	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	986	0.183376015681811	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	982	0.18263209675409575	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	714	0.13278952859717347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.859797319288144E-4	0.0	0.0	0.05058648708463752	0.0
2	1.859797319288144E-4	0.0	0.0	0.19918429289576023	0.0
3	1.859797319288144E-4	0.0	0.0	0.2802714560167233	0.0
4	1.859797319288144E-4	0.0	0.0	0.3981826060595916	0.0
5	1.859797319288144E-4	0.0	0.0	0.706351021865637	0.0
6	1.859797319288144E-4	0.0	0.0	0.9713721398641976	0.0
7	1.859797319288144E-4	0.0	0.0	1.2157495076186597	0.0
8	1.859797319288144E-4	0.0	0.0	1.5443756939368747	0.0
9	1.859797319288144E-4	0.0	0.0	1.7019005268805805	0.0
10	1.859797319288144E-4	0.0	0.0	2.035362186228945	0.0
11	1.859797319288144E-4	0.0	0.0	2.532857969138523	0.0
12	1.859797319288144E-4	0.0	0.0	2.8702252028573927	0.0
13	1.859797319288144E-4	0.0	0.0	3.0117557788552203	0.0
14	1.859797319288144E-4	0.0	0.0	3.086519631090604	0.0
15	1.859797319288144E-4	0.0	0.0	3.1940159161454584	0.0
16	1.859797319288144E-4	0.0	0.0	3.392084330649646	0.0
17	1.859797319288144E-4	0.0	0.0	3.657291428380135	0.0
18	1.859797319288144E-4	0.0	0.0	3.91691913415276	0.0
19	1.859797319288144E-4	0.0	0.0	4.10680444045208	0.0
20	1.859797319288144E-4	0.0	0.0	4.301711199513477	0.0
21	1.859797319288144E-4	0.0	0.0	4.543856810484793	0.0
22	1.859797319288144E-4	0.0	0.0	4.820036712399083	0.0
23	1.859797319288144E-4	0.0	0.0	5.093240938602511	0.0
24	1.859797319288144E-4	0.0	0.0	5.311209184423082	0.0
25	1.859797319288144E-4	0.0	0.0	5.528061551852079	0.0
26	1.859797319288144E-4	0.0	0.0	5.721852432521904	0.0
27	1.859797319288144E-4	0.0	0.0	5.9321955093333925	0.0
28	1.859797319288144E-4	0.0	0.0	6.160950579605834	0.0
29	1.859797319288144E-4	0.0	0.0	6.377802947034832	0.0
30	1.859797319288144E-4	0.0	0.0	6.638174571735172	0.0
31	1.859797319288144E-4	0.0	0.0	6.878646365119129	0.0
32	1.859797319288144E-4	0.0	0.0	7.130276942418815	0.0
33	1.859797319288144E-4	0.0	0.0	7.36479738438105	0.0
34	1.859797319288144E-4	0.0	0.0	7.609732691331299	0.0
35	3.719594638576288E-4	0.0	0.0	7.874753809329859	0.0
36	3.719594638576288E-4	0.0	0.0	8.139960907060349	0.0
37	3.719594638576288E-4	0.0	0.0	8.41744266709814	0.0
38	3.719594638576288E-4	0.0	0.0	8.705525271855873	0.0
39	3.719594638576288E-4	0.0	0.0	8.996769532056396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.029935E-4	45.0	2
TTTCGCG	20	7.029935E-4	45.0	1
TAGCGCG	25	3.8880273E-5	45.0	1
TAGCACG	20	7.029935E-4	45.0	1
TACGCGC	20	7.029935E-4	45.0	34
TACTGCG	20	7.029935E-4	45.0	1
AGCGATT	20	7.029935E-4	45.0	22
CGTAAGG	45	3.8380676E-10	45.0	2
ACGTAAG	20	7.029935E-4	45.0	1
TGCAACG	25	3.8880273E-5	45.0	1
CGACGTT	30	2.1633386E-6	44.999996	27
TCGACGT	30	2.1633386E-6	44.999996	26
CGTTATT	575	0.0	43.826088	1
CGTTTTT	9315	0.0	43.719807	1
CACGCCG	60	3.6379788E-12	41.249996	26
CCCTCGT	115	0.0	41.08696	14
CTAGACG	40	3.4536424E-7	39.375	1
CTGTACG	40	3.4536424E-7	39.375	1
TATTGCG	40	3.4536424E-7	39.375	1
AGTTACG	40	3.4536424E-7	39.375	1
>>END_MODULE