FastQCFastQC Report
Sat 18 Jun 2016
SRR3550890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361637
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118473.275936920171332No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.19771207039102748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAG302.1613596E-645.0000041
TTAGGCG207.026867E-445.01
GCGACAA207.026867E-445.01
ACGTTCA207.026867E-445.029
TAAACGG207.026867E-445.02
CGTCTGG207.026867E-445.02
AAAGTCG253.8854858E-545.01
AACGGGT253.8854858E-545.04
CGGGCAC406.7921064E-945.06
AGCGCAT207.026867E-445.023
TCGTCAA207.026867E-445.017
GGTATAC253.8854858E-545.08
CACGTGA253.8854858E-545.043
CGACTGG253.8854858E-545.02
GCCAATC253.8854858E-545.015
GACTCAC207.026867E-445.09
GTACGAG253.8854858E-545.01
GACTACG207.026867E-445.01
CGTTTTT61900.043.9458771
CGTTATT4250.043.9411771