##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550890_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361637 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.287335090159466 33.0 31.0 34.0 31.0 34.0 2 32.374740416494994 34.0 31.0 34.0 31.0 34.0 3 32.18484281199103 34.0 31.0 34.0 30.0 34.0 4 35.95184121093804 37.0 35.0 37.0 35.0 37.0 5 33.91303710626955 37.0 35.0 37.0 33.0 37.0 6 34.89273498010436 37.0 35.0 37.0 32.0 37.0 7 35.95662777868415 37.0 35.0 37.0 33.0 37.0 8 36.00035118088027 37.0 35.0 37.0 35.0 37.0 9 37.99549824824341 39.0 38.0 39.0 35.0 39.0 10 37.64158534663212 39.0 37.0 39.0 35.0 39.0 11 37.55026449174172 39.0 37.0 39.0 35.0 39.0 12 37.59394641588112 39.0 37.0 39.0 35.0 39.0 13 37.60570682756466 39.0 37.0 39.0 35.0 39.0 14 38.90429076670805 40.0 38.0 41.0 35.0 41.0 15 39.02407939453098 40.0 38.0 41.0 35.0 41.0 16 38.98510384722802 40.0 38.0 41.0 35.0 41.0 17 38.9604271686802 40.0 38.0 41.0 35.0 41.0 18 38.65549155645025 40.0 38.0 41.0 35.0 41.0 19 38.290562082972706 40.0 37.0 41.0 35.0 41.0 20 38.02434761929781 40.0 35.0 41.0 35.0 41.0 21 37.881104532998556 40.0 35.0 41.0 34.0 41.0 22 37.86796704983174 40.0 35.0 41.0 34.0 41.0 23 37.87027599498945 39.0 35.0 41.0 34.0 41.0 24 37.798875668142365 40.0 35.0 41.0 34.0 41.0 25 37.61694185052968 39.0 35.0 41.0 34.0 41.0 26 37.65521780127587 39.0 35.0 41.0 34.0 41.0 27 37.66126806715021 39.0 35.0 41.0 34.0 41.0 28 37.531049090662734 39.0 35.0 41.0 34.0 41.0 29 37.43805805268819 39.0 35.0 41.0 33.0 41.0 30 37.158523602396876 39.0 35.0 41.0 33.0 41.0 31 36.893791287948964 39.0 35.0 41.0 33.0 41.0 32 36.42223832185313 39.0 35.0 41.0 31.0 41.0 33 35.82739044953918 39.0 35.0 41.0 25.0 41.0 34 35.316731971562646 39.0 35.0 41.0 21.0 41.0 35 34.978359515204474 39.0 35.0 41.0 18.0 41.0 36 34.720083951586815 39.0 35.0 41.0 16.0 41.0 37 34.58700298918529 39.0 35.0 41.0 15.0 41.0 38 34.44514526998067 39.0 35.0 41.0 15.0 41.0 39 34.341447362963414 39.0 35.0 41.0 15.0 41.0 40 34.24701841902239 39.0 35.0 41.0 15.0 41.0 41 34.08348979778065 39.0 34.0 41.0 12.0 41.0 42 33.98930142656863 39.0 34.0 41.0 10.0 41.0 43 33.878629675613944 39.0 34.0 41.0 10.0 41.0 44 33.71181046187199 39.0 34.0 41.0 10.0 41.0 45 33.69456388588557 39.0 34.0 41.0 10.0 41.0 46 33.59943811059156 38.0 33.0 41.0 10.0 41.0 47 33.56366743447158 38.0 33.0 41.0 10.0 41.0 48 33.489725885349124 38.0 33.0 41.0 10.0 41.0 49 33.367210766597445 38.0 33.0 41.0 10.0 41.0 50 33.31088356556437 38.0 33.0 40.0 10.0 41.0 51 32.48218240943266 37.0 31.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 8.0 10 7.0 11 8.0 12 7.0 13 4.0 14 9.0 15 27.0 16 36.0 17 54.0 18 127.0 19 273.0 20 421.0 21 696.0 22 1085.0 23 1802.0 24 3427.0 25 6140.0 26 9946.0 27 11858.0 28 10886.0 29 9129.0 30 7813.0 31 7587.0 32 7861.0 33 10081.0 34 16556.0 35 23450.0 36 21680.0 37 30662.0 38 58244.0 39 121675.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.32042904901877 17.471387053868934 21.561123446992426 27.64706045011987 2 43.27460962235612 20.73073275135011 22.03978022160343 13.954877404690338 3 27.426950229097137 19.363339481303075 40.3971385671267 12.812571722473088 4 23.979570674460852 22.137944955853524 39.15888031368472 14.723604056000909 5 20.06901948639105 29.950751720648057 36.13070565235305 13.849523140607847 6 22.999305933850795 28.576445441146785 36.628718853435906 11.795529771566516 7 68.61521359816611 4.760574830562138 23.635026283261944 2.9891852880098 8 70.54228411362774 4.412712194825198 22.68628486576318 2.3587188257838663 9 64.63663839706668 7.421253909306847 24.248348482041386 3.693759211585098 10 31.550975149113615 29.40655961641093 29.84124965089302 9.201215583582432 11 23.26587157840597 21.326081125548548 41.84251058381748 13.565536712228008 12 22.798828659678076 20.53053199755556 41.97109255966618 14.699546783100182 13 19.555521144130715 20.85986776795516 43.15155805407079 16.43305303384333 14 15.839916822670247 23.888595470043164 42.78240334921482 17.489084358071768 15 16.08491387772822 23.870345125083993 45.19532016911986 14.849420828067924 16 18.107660444036423 22.688497028788536 44.444843862768465 14.758998664406572 17 17.277269748394108 22.727762922488573 42.66128742357668 17.333679905540638 18 17.74210050409665 23.188169352140406 42.96352419691569 16.10620594684725 19 19.423067882987638 23.526906815397762 41.25435173945144 15.795673562163165 20 20.990938427207393 23.42957164228218 41.68378788674831 13.895702043762114 21 20.104690615174885 22.392067183391077 42.477401372094114 15.025840829339918 22 19.20212810083039 21.864742822222283 42.328909929017215 16.604219147930106 23 17.955574236043326 22.830628503167542 42.83715438409234 16.3766428766968 24 17.37819968642589 23.191211076300267 43.1020609063785 16.328528330895345 25 17.27312194272157 24.395733843605605 41.994043751054235 16.337100462618594 26 17.315429560581467 25.43434438400938 41.73217895292793 15.518047102481217 27 17.79546893708332 24.511872402436698 42.463851873563826 15.228806786916163 28 16.294239804002355 25.238291435887366 42.69004554290628 15.777423217203992 29 17.035037897117828 23.884724184748794 43.07523842969608 16.0049994884373 30 18.231818093834427 23.15719906978545 41.70950428191806 16.90147855446207 31 18.311179442369006 24.407347699488714 40.35372486775413 16.927747990388152 32 18.897126123709686 25.14012670163728 40.28044696753928 15.682300207113764 33 19.22480277184027 25.68929617268145 38.447393380655186 16.638507674823096 34 18.2113555858499 26.865060820657177 38.2007372033282 16.722846390164722 35 19.250242646631843 25.954202694967606 37.079170549473645 17.71638410892691 36 19.670000580692797 27.47810649905845 35.629097686353994 17.22279523389476 37 19.51266048551448 27.94293725476099 36.137065621050944 16.407336638673588 38 18.942475465729448 27.974737098250458 35.43663950314818 17.646147932871912 39 19.78448001725487 26.5949004111858 36.02203314373253 17.5985864278268 40 20.68178864441415 26.73205451875776 36.0187148991945 16.567441937633596 41 18.591294585454477 27.504375934984527 35.49775050672359 18.406578972837405 42 19.55690374602156 27.7880858429862 34.478772913169834 18.176237497822402 43 19.9362897048698 27.416442454726702 34.56781247494034 18.079455365463158 44 19.266833869322 28.174937852045005 34.21193074823649 18.3462975303965 45 18.89961480711321 27.53009233015427 34.17100573226744 19.39928713046508 46 18.91537646866886 28.32094061171838 34.37369516946551 18.389987750147245 47 19.503258792656723 27.105357029286274 35.1548652377937 18.236518940263302 48 19.94320271432403 25.83612849348933 35.604210852318765 18.61645793986788 49 18.066458907689203 26.76966129018878 36.07484853596286 19.08903126615916 50 18.292376056653495 27.596457220914893 35.06886740018306 19.042299322248553 51 18.364824395733844 26.332482572303167 34.85649974974906 20.446193282213933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 252.0 1 667.5 2 1083.0 3 11571.0 4 22059.0 5 14908.0 6 7757.0 7 7333.5 8 6910.0 9 6542.5 10 6175.0 11 5966.0 12 5757.0 13 5386.0 14 5015.0 15 4668.5 16 4322.0 17 4030.0 18 3738.0 19 3551.5 20 3365.0 21 3373.0 22 3381.0 23 3308.5 24 3236.0 25 3773.0 26 5162.0 27 6014.0 28 6846.0 29 7678.0 30 8152.5 31 8627.0 32 9727.0 33 10827.0 34 11930.5 35 13034.0 36 14052.5 37 15071.0 38 15751.5 39 16432.0 40 17441.0 41 18450.0 42 19967.5 43 21485.0 44 22300.5 45 23116.0 46 23204.0 47 23292.0 48 23320.0 49 23348.0 50 21615.5 51 19883.0 52 17311.5 53 14740.0 54 13488.0 55 12236.0 56 11172.0 57 10108.0 58 10052.5 59 9997.0 60 9850.5 61 9704.0 62 8821.0 63 7938.0 64 6542.5 65 5147.0 66 3943.0 67 2739.0 68 2198.5 69 1658.0 70 1370.0 71 1082.0 72 902.0 73 722.0 74 607.5 75 360.0 76 227.0 77 183.0 78 139.0 79 83.0 80 27.0 81 27.0 82 27.0 83 24.5 84 22.0 85 13.0 86 4.0 87 3.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 2.0 94 4.0 95 3.5 96 3.0 97 1.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 361637.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.72832105188351 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61750354140075 25.841797576381865 2 7.03877083379403 4.748118449856777 3 2.879658497392438 2.9137813895950977 4 1.6776190441095282 2.2633309488992035 5 1.2153302551799452 2.0495524515388355 6 1.0410084296823277 2.1066879920425587 7 0.8720857666483758 2.0589792105606066 8 0.7888943629980706 2.128646588097604 9 0.6818383933431998 2.069753781256189 >10 7.049770535061094 46.259984376352 >50 0.10458133670397184 2.113912041326229 >100 0.03129205350197583 1.9564310102617208 >500 8.234750921572587E-4 0.19858719084854207 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 8.234750921572587E-4 3.2904369929827664 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11847 3.275936920171332 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 715 0.19771207039102748 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.530407563385383E-4 0.0 0.0 0.06083448319723922 0.0 2 5.530407563385383E-4 0.0 0.0 0.3011306918263341 0.0 3 5.530407563385383E-4 0.0 0.0 0.3901702535968388 0.0 4 5.530407563385383E-4 0.0 0.0 0.49718363994834597 0.0 5 5.530407563385383E-4 0.0 0.0 0.8193298805155446 0.0 6 5.530407563385383E-4 0.0 0.0 0.9833064647699212 0.0 7 5.530407563385383E-4 0.0 0.0 1.1547490992348681 0.0 8 5.530407563385383E-4 0.0 0.0 1.3942157467294551 0.0 9 5.530407563385383E-4 0.0 0.0 1.4890622364415145 0.0 10 5.530407563385383E-4 0.0 0.0 1.761711329316414 0.0 11 5.530407563385383E-4 0.0 0.0 2.19087095623512 0.0 12 5.530407563385383E-4 0.0 0.0 2.506380707726256 0.0 13 5.530407563385383E-4 0.0 0.0 2.6109054106742398 0.0 14 5.530407563385383E-4 0.0 0.0 2.6604025583665387 0.0 15 5.530407563385383E-4 0.0 0.0 2.748059518246197 0.0 16 5.530407563385383E-4 0.0 0.0 2.9457715886372244 0.0 17 5.530407563385383E-4 0.0 0.0 3.20487118298183 0.0 18 5.530407563385383E-4 0.0 0.0 3.472266388671513 0.0 19 5.530407563385383E-4 0.0 0.0 3.6547698382632308 0.0 20 5.530407563385383E-4 0.0 0.0 3.8134925353323914 0.0 21 5.530407563385383E-4 0.0 0.0 4.028348869169913 0.0 22 5.530407563385383E-4 0.0 0.0 4.279705892925779 0.0 23 5.530407563385383E-4 0.0 0.0 4.522490784958397 0.0 24 5.530407563385383E-4 0.0 0.0 4.719649814593087 0.0 25 5.530407563385383E-4 0.0 0.0 4.885009000738309 0.0 26 5.530407563385383E-4 0.0 0.0 5.046220381210993 0.0 27 5.530407563385383E-4 0.0 0.0 5.215174332272417 0.0 28 5.530407563385383E-4 0.0 0.0 5.400996026402166 0.0 29 5.530407563385383E-4 0.0 0.0 5.596772454146008 0.0 30 5.530407563385383E-4 0.0 0.0 5.816329634412408 0.0 31 5.530407563385383E-4 0.0 0.0 6.005193052702019 0.0 32 5.530407563385383E-4 0.0 0.0 6.213412897463479 0.0 33 5.530407563385383E-4 0.0 0.0 6.387620735710118 0.0 34 5.530407563385383E-4 0.0 0.0 6.57233634832719 0.0 35 5.530407563385383E-4 0.0 0.0 6.770601459474556 0.0 36 5.530407563385383E-4 0.0 0.0 6.990988200875464 0.0 37 5.530407563385383E-4 0.0 0.0 7.205014973578478 0.0 38 5.530407563385383E-4 0.0 0.0 7.426231276113893 0.0 39 5.530407563385383E-4 0.0 0.0 7.645235415623954 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAAAG 30 2.1613596E-6 45.000004 1 TTAGGCG 20 7.026867E-4 45.0 1 GCGACAA 20 7.026867E-4 45.0 1 ACGTTCA 20 7.026867E-4 45.0 29 TAAACGG 20 7.026867E-4 45.0 2 CGTCTGG 20 7.026867E-4 45.0 2 AAAGTCG 25 3.8854858E-5 45.0 1 AACGGGT 25 3.8854858E-5 45.0 4 CGGGCAC 40 6.7921064E-9 45.0 6 AGCGCAT 20 7.026867E-4 45.0 23 TCGTCAA 20 7.026867E-4 45.0 17 GGTATAC 25 3.8854858E-5 45.0 8 CACGTGA 25 3.8854858E-5 45.0 43 CGACTGG 25 3.8854858E-5 45.0 2 GCCAATC 25 3.8854858E-5 45.0 15 GACTCAC 20 7.026867E-4 45.0 9 GTACGAG 25 3.8854858E-5 45.0 1 GACTACG 20 7.026867E-4 45.0 1 CGTTTTT 6190 0.0 43.945877 1 CGTTATT 425 0.0 43.941177 1 >>END_MODULE