FastQCFastQC Report
Sat 18 Jun 2016
SRR3550887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592204
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176242.9760015129921444No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC12490.21090705229954546No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC11100.18743541077061282No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.17781034913644622No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9620.16244402266786445No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT7860.13272453411324475No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7670.12951618023518924No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6620.11178580354067179No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6500.10975947477558409No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6120.10334276701947302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA207.0305134E-445.06
TCGCAAG207.0305134E-445.01
CGGCCTA207.0305134E-445.045
ACGGGCC253.8885075E-545.05
TCGTGCG207.0305134E-445.01
AGTTGCG351.210592E-745.01
GCGATCG207.0305134E-445.09
GGGTTCG207.0305134E-445.08
TGCTACG207.0305134E-445.01
ACACGAC551.8189894E-1244.99999626
TATCGAG302.1637134E-644.9999961
CGTTTTT85500.043.6578941
CGTTATT5650.043.008851
TCGTGTA700.041.78571317
GCCCTCG700.041.78571313
AACACGT700.041.78571341
AACGGGC556.002665E-1140.909094
CGTGCGG451.9255822E-840.02
ACGGGTA451.9255822E-840.05
TAGGGCG850.039.7058835