##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592204
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06059398450534	33.0	31.0	34.0	30.0	34.0
2	32.14734618476066	33.0	31.0	34.0	30.0	34.0
3	31.99367447703832	33.0	31.0	34.0	30.0	34.0
4	35.78447798393797	37.0	35.0	37.0	35.0	37.0
5	33.711535214216724	37.0	35.0	37.0	32.0	37.0
6	34.71053893590722	37.0	35.0	37.0	31.0	37.0
7	35.804457585561735	37.0	35.0	37.0	33.0	37.0
8	35.819378119701994	37.0	35.0	37.0	35.0	37.0
9	37.78897812240377	39.0	38.0	39.0	35.0	39.0
10	37.40778853233007	39.0	37.0	39.0	34.0	39.0
11	37.33257120857002	39.0	37.0	39.0	34.0	39.0
12	37.36266219073157	39.0	37.0	39.0	35.0	39.0
13	37.37909402840913	39.0	37.0	39.0	35.0	39.0
14	38.58875319991084	40.0	38.0	41.0	34.0	41.0
15	38.68700143869342	40.0	38.0	41.0	35.0	41.0
16	38.64305543360059	40.0	38.0	41.0	34.0	41.0
17	38.62873604366063	40.0	38.0	41.0	34.0	41.0
18	38.37361280909957	39.0	38.0	41.0	34.0	41.0
19	38.046186786985565	39.0	37.0	41.0	34.0	41.0
20	37.84629452013158	39.0	35.0	41.0	34.0	41.0
21	37.70774935664062	39.0	35.0	41.0	34.0	41.0
22	37.727833651917244	39.0	35.0	41.0	34.0	41.0
23	37.743753841581615	39.0	35.0	41.0	34.0	41.0
24	37.659191764999896	39.0	35.0	41.0	34.0	41.0
25	37.46460847951044	39.0	35.0	41.0	33.0	41.0
26	37.51899514356539	39.0	35.0	41.0	33.0	41.0
27	37.53192987551587	39.0	35.0	41.0	34.0	41.0
28	37.414426447643045	39.0	35.0	41.0	33.0	41.0
29	37.35628432094346	39.0	35.0	41.0	33.0	41.0
30	37.07342402280295	39.0	35.0	41.0	33.0	41.0
31	36.85090272946485	39.0	35.0	41.0	32.0	41.0
32	36.475591181417215	39.0	35.0	41.0	31.0	41.0
33	35.99150123943776	39.0	35.0	41.0	28.0	41.0
34	35.57199377241626	39.0	35.0	41.0	23.0	41.0
35	35.27616328157189	39.0	35.0	41.0	21.0	41.0
36	35.0428956913496	39.0	35.0	41.0	20.0	41.0
37	34.92945167543617	39.0	35.0	41.0	18.0	41.0
38	34.7851686243254	39.0	35.0	41.0	18.0	41.0
39	34.6769153872652	39.0	35.0	41.0	18.0	41.0
40	34.592750133399974	39.0	35.0	41.0	18.0	41.0
41	34.471263280896444	39.0	35.0	41.0	16.0	41.0
42	34.40785438801494	39.0	35.0	41.0	15.0	41.0
43	34.328396971313936	39.0	34.0	41.0	15.0	41.0
44	34.16843688999061	39.0	34.0	41.0	15.0	41.0
45	34.1174122430784	38.0	34.0	41.0	15.0	41.0
46	34.01650951361355	38.0	34.0	41.0	15.0	41.0
47	34.00872672254831	38.0	34.0	41.0	15.0	41.0
48	33.94298924019426	38.0	34.0	40.0	15.0	41.0
49	33.86038932530006	38.0	34.0	40.0	15.0	41.0
50	33.779214594970654	38.0	33.0	40.0	15.0	41.0
51	32.86869896184423	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	7.0
11	14.0
12	14.0
13	17.0
14	12.0
15	34.0
16	71.0
17	111.0
18	200.0
19	423.0
20	734.0
21	1116.0
22	1834.0
23	3034.0
24	5136.0
25	9275.0
26	14367.0
27	16681.0
28	15475.0
29	13683.0
30	12940.0
31	13282.0
32	14529.0
33	18672.0
34	30461.0
35	41237.0
36	39979.0
37	57352.0
38	101404.0
39	180002.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.041647810551765	18.831517517612173	21.944971665169437	25.18186300666662
2	41.08668634457045	20.56132684007538	23.180187908220816	15.171798907133352
3	27.504542353648404	20.209421077871813	38.58687209137392	13.699164477105864
4	25.24282173034968	22.248583258471744	37.087051083748165	15.421543927430411
5	20.527554693990584	30.653794976055547	33.93459010746297	14.8840602224909
6	22.7793125341943	29.386832915684458	35.37429669505778	12.459557855063458
7	68.74337221633085	5.713402813895212	21.80498611964796	3.7382388501259705
8	69.36157135041303	5.317593261781413	21.470979594869334	3.849855792936218
9	64.17281882594511	7.762358916859731	22.985153764581124	5.079668492614032
10	32.91348927058919	27.506906403874343	29.258161039101388	10.321443286435079
11	23.7554964167753	22.48583258471743	38.42915616915793	15.329514829349348
12	22.423185253730132	20.605737212176887	40.401280639779536	16.569796894313445
13	20.269366637172325	22.091205057716596	41.016609141444505	16.622819163666573
14	17.21940412425448	23.812740204389026	40.71046463718584	18.25739103417066
15	16.598672079215945	24.37268238647493	42.60643291838623	16.42221261592289
16	18.606088442496166	24.169036345583617	40.609485920392295	16.61538929152792
17	18.110819920162648	24.454917562191408	39.10324820501044	18.33101431263551
18	18.749282341895697	24.01402219505441	40.193244219897196	17.043451243152695
19	19.148806830078826	24.622089685311146	38.98555227590492	17.243551208705107
20	20.292331696509986	24.68490587702886	39.785445555923296	15.237316870537857
21	20.177675260552107	24.32540138195622	39.8094237796435	15.687499577848174
22	19.412567291001075	22.516565237654593	39.1417484515471	18.929119019797234
23	18.156581178107544	23.726621231872798	39.99010476119715	18.1266928288225
24	17.912577422644897	22.848038851476858	41.30417896535653	17.93520476052171
25	17.26651626804277	25.317289312466652	38.849788248644046	18.566406170846534
26	17.03382618151853	25.73285557004005	38.365664534518515	18.867653713922905
27	18.136317890456667	24.7281342240174	39.05123910003985	18.084308785486083
28	15.905836502286375	24.762412952293467	40.66689856873645	18.66485197668371
29	17.46104382949119	22.881135554639958	39.64934380720157	20.008476808667282
30	17.487554964167753	23.855461969186294	39.527088638374615	19.12989442827134
31	18.444995305671693	24.687776509446067	38.08062762156284	18.786600563319396
32	18.46981783304402	24.915400774057588	37.45685608337667	19.15792530952172
33	17.907342740001756	25.29888349285044	37.37478976839062	19.418983998757184
34	17.658442023356816	25.0866255547075	37.536051765945516	19.718880655990166
35	18.536348960831063	24.659576767465268	34.9138134831916	21.890260788512066
36	18.278160904012804	26.461320761089084	35.38696124983958	19.873557085058525
37	18.624831983573227	27.282997075332148	35.83748167861075	18.254689262483872
38	18.430135561394383	27.032576612113395	34.549412026936665	19.987875799555557
39	19.582947767998863	25.428906255276896	35.93812267394344	19.0500233027808
40	19.439416147138484	25.604690275648256	35.76875536132819	19.187138215885067
41	17.508493694740327	26.07023930942716	34.54417734429352	21.877089651539
42	17.963066781041668	26.325219012367363	34.10125564839143	21.61045855819954
43	19.521651322854964	25.127489851470102	34.49318140370548	20.857677421969456
44	18.79487473911017	26.403232669823236	33.543846377261886	21.2580462138047
45	19.11165746938555	25.78790416815827	33.088428987308426	22.012009375147752
46	18.783054487980493	26.618867822574654	33.891192899744006	20.706884789700847
47	18.121458146179357	25.267137675530734	36.00431608026964	20.607088098020277
48	18.2295290136507	24.672747904438335	35.61289690714686	21.484826174764102
49	18.46306340382706	24.82708661204585	36.30438159823304	20.40546838589405
50	17.23426386853179	24.34363834084201	36.29931577632032	22.12278201430588
51	17.551384320268014	23.480084565453797	34.75879933266239	24.209731781615794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	318.0
1	942.0
2	1566.0
3	16453.0
4	31340.0
5	20847.5
6	10355.0
7	9606.5
8	8858.0
9	8529.5
10	8201.0
11	7859.0
12	7517.0
13	7203.5
14	6890.0
15	6508.0
16	6126.0
17	5706.0
18	5286.0
19	5016.5
20	4747.0
21	4718.5
22	4690.0
23	4729.5
24	4769.0
25	5127.0
26	6608.0
27	7731.0
28	10035.5
29	12340.0
30	12658.0
31	12976.0
32	14128.0
33	15280.0
34	17038.5
35	18797.0
36	19985.5
37	21174.0
38	22591.5
39	24009.0
40	26253.5
41	28498.0
42	30519.5
43	32541.0
44	34771.0
45	37001.0
46	41127.5
47	45254.0
48	47530.0
49	49806.0
50	46835.0
51	43864.0
52	38455.0
53	33046.0
54	28871.0
55	24696.0
56	21938.5
57	19181.0
58	17476.5
59	15772.0
60	14840.0
61	13908.0
62	12552.0
63	11196.0
64	9412.0
65	7628.0
66	5988.0
67	4348.0
68	3465.0
69	2582.0
70	2184.0
71	1786.0
72	1502.0
73	1218.0
74	924.0
75	547.0
76	464.0
77	334.0
78	204.0
79	130.0
80	56.0
81	55.0
82	54.0
83	32.0
84	10.0
85	6.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.81582179491151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28926613747787	28.08652026981962
2	9.076548445396076	6.683211801571661
3	3.3452452400684405	3.6947385785590696
4	1.775441631385252	2.614573708333856
5	1.163983168595658	2.1426498453647094
6	0.949649181873515	2.0977269028523304
7	0.727194727307724	1.8740590043532308
8	0.6139928292487952	1.8083720467981739
9	0.5687582427873917	1.8845371899762862
>10	5.28777224310184	38.53493490720889
>50	0.16346832398470168	3.754352543322561
>100	0.033154138948093125	2.3245589304092418
>500	0.0036837932164547915	0.9335870791834667
>1k	0.001381422456170547	0.5784272951105844
>5k	0.0	0.0
>10k+	4.6047415205684893E-4	2.9877498971362657
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17624	2.9760015129921444	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1249	0.21090705229954546	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1110	0.18743541077061282	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1053	0.17781034913644622	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	962	0.16244402266786445	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	786	0.13272453411324475	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	767	0.12951618023518924	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	662	0.11178580354067179	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	650	0.10975947477558409	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	612	0.10334276701947302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.065821912719266E-4	0.0	0.0	0.14386934232122714	0.0
2	5.065821912719266E-4	0.0	0.0	0.7367393668398052	0.0
3	5.065821912719266E-4	0.0	0.0	0.9815874259545697	0.0
4	5.065821912719266E-4	0.0	0.0	1.2823283868396702	0.0
5	5.065821912719266E-4	0.0	0.0	2.2022816461894887	0.0
6	5.065821912719266E-4	0.0	0.0	2.757833449284368	0.0
7	5.065821912719266E-4	0.0	0.0	3.338883222673268	0.0
8	5.065821912719266E-4	0.0	0.0	4.167820548324563	0.0
9	5.065821912719266E-4	0.0	0.0	4.444245564028612	0.0
10	5.065821912719266E-4	0.0	0.0	5.1352236729235194	0.0
11	5.065821912719266E-4	0.0	0.0	6.221504751740954	0.0
12	5.065821912719266E-4	0.0	0.0	7.06445751801744	0.0
13	5.065821912719266E-4	0.0	0.0	7.39745087841352	0.0
14	5.065821912719266E-4	0.0	0.0	7.520550350892598	0.0
15	5.065821912719266E-4	0.0	0.0	7.750538665730052	0.0
16	6.754429216959021E-4	0.0	0.0	8.200214790849099	0.0
17	8.443036521198777E-4	0.0	0.0	8.782277728620542	0.0
18	8.443036521198777E-4	0.0	0.0	9.408919899223916	0.0
19	8.443036521198777E-4	0.0	0.0	9.804729451337714	0.0
20	8.443036521198777E-4	0.0	0.0	10.216749633572215	0.0
21	8.443036521198777E-4	0.0	0.0	10.753220174129185	0.0
22	8.443036521198777E-4	0.0	0.0	11.33528311190063	0.0
23	8.443036521198777E-4	0.0	0.0	11.889484029152117	0.0
24	8.443036521198777E-4	0.0	0.0	12.32582015656767	0.0
25	8.443036521198777E-4	0.0	0.0	12.72129198722062	0.0
26	8.443036521198777E-4	0.0	0.0	13.057493701494755	0.0
27	8.443036521198777E-4	0.0	0.0	13.415478449993584	0.0
28	8.443036521198777E-4	0.0	0.0	13.779879906248523	0.0
29	8.443036521198777E-4	0.0	0.0	14.177209204936137	0.0
30	8.443036521198777E-4	0.0	0.0	14.614727357464657	0.0
31	0.0011820251129678286	0.0	0.0	15.022863742899407	0.0
32	0.0013508858433918041	0.0	0.0	15.41377633383091	0.0
33	0.0013508858433918041	0.0	0.0	15.792193230711039	0.0
34	0.0015197465738157796	0.0	0.0	16.1694281024782	0.0
35	0.0015197465738157796	0.0	0.0	16.576044741339132	0.0
36	0.0015197465738157796	0.0	0.0	16.972867457835477	0.0
37	0.0015197465738157796	0.0	0.0	17.357532201741293	0.0
38	0.0015197465738157796	0.0	0.0	17.725648594065557	0.0
39	0.0015197465738157796	0.0	0.0	18.11521029915367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0305134E-4	45.0	6
TCGCAAG	20	7.0305134E-4	45.0	1
CGGCCTA	20	7.0305134E-4	45.0	45
ACGGGCC	25	3.8885075E-5	45.0	5
TCGTGCG	20	7.0305134E-4	45.0	1
AGTTGCG	35	1.210592E-7	45.0	1
GCGATCG	20	7.0305134E-4	45.0	9
GGGTTCG	20	7.0305134E-4	45.0	8
TGCTACG	20	7.0305134E-4	45.0	1
ACACGAC	55	1.8189894E-12	44.999996	26
TATCGAG	30	2.1637134E-6	44.999996	1
CGTTTTT	8550	0.0	43.657894	1
CGTTATT	565	0.0	43.00885	1
TCGTGTA	70	0.0	41.785713	17
GCCCTCG	70	0.0	41.785713	13
AACACGT	70	0.0	41.785713	41
AACGGGC	55	6.002665E-11	40.90909	4
CGTGCGG	45	1.9255822E-8	40.0	2
ACGGGTA	45	1.9255822E-8	40.0	5
TAGGGCG	85	0.0	39.705883	5
>>END_MODULE