##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464489
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.304205266432575	33.0	31.0	34.0	31.0	34.0
2	32.381714098719236	34.0	31.0	34.0	31.0	34.0
3	32.22375772085022	34.0	31.0	34.0	30.0	34.0
4	35.967092869798854	37.0	35.0	37.0	35.0	37.0
5	33.95346929636654	37.0	35.0	37.0	33.0	37.0
6	34.90009666536775	37.0	35.0	37.0	32.0	37.0
7	35.93266148391027	37.0	35.0	37.0	33.0	37.0
8	35.9717065420279	37.0	35.0	37.0	35.0	37.0
9	37.964707452706094	39.0	38.0	39.0	35.0	39.0
10	37.602061620404356	39.0	37.0	39.0	35.0	39.0
11	37.51475923003559	39.0	37.0	39.0	35.0	39.0
12	37.528124455046296	39.0	37.0	39.0	35.0	39.0
13	37.55097967874374	39.0	37.0	39.0	35.0	39.0
14	38.85143243435259	40.0	38.0	41.0	35.0	41.0
15	38.971964890449506	40.0	38.0	41.0	35.0	41.0
16	38.909358456282064	40.0	38.0	41.0	35.0	41.0
17	38.875271534955615	40.0	38.0	41.0	35.0	41.0
18	38.6004684718045	40.0	38.0	41.0	35.0	41.0
19	38.291804542195834	40.0	37.0	41.0	35.0	41.0
20	38.06372594399437	40.0	35.0	41.0	35.0	41.0
21	37.92957206736866	40.0	35.0	41.0	34.0	41.0
22	37.92603269399275	40.0	35.0	41.0	34.0	41.0
23	37.92997896613267	40.0	35.0	41.0	34.0	41.0
24	37.84647645046492	40.0	35.0	41.0	34.0	41.0
25	37.64121432369765	39.0	35.0	41.0	34.0	41.0
26	37.69329305968495	40.0	35.0	41.0	34.0	41.0
27	37.69521990832937	40.0	35.0	41.0	34.0	41.0
28	37.570194342600146	40.0	35.0	41.0	34.0	41.0
29	37.507775211038364	40.0	35.0	41.0	33.0	41.0
30	37.24125652060651	40.0	35.0	41.0	33.0	41.0
31	37.00962563160807	40.0	35.0	41.0	33.0	41.0
32	36.60535340987623	40.0	35.0	41.0	31.0	41.0
33	36.10618550708413	40.0	35.0	41.0	28.0	41.0
34	35.67796869247711	40.0	35.0	41.0	23.0	41.0
35	35.37413587835234	40.0	35.0	41.0	21.0	41.0
36	35.1484362385331	39.0	35.0	41.0	18.0	41.0
37	35.024409620034056	39.0	35.0	41.0	18.0	41.0
38	34.88626210739113	39.0	35.0	41.0	18.0	41.0
39	34.78880016534299	39.0	35.0	41.0	18.0	41.0
40	34.67342176025697	39.0	35.0	41.0	17.0	41.0
41	34.519013367377916	39.0	35.0	41.0	15.0	41.0
42	34.446975062918604	39.0	35.0	41.0	15.0	41.0
43	34.369369349973844	39.0	35.0	41.0	15.0	41.0
44	34.2152709752007	39.0	34.0	41.0	15.0	41.0
45	34.182680321816015	39.0	34.0	41.0	15.0	41.0
46	34.1090897739236	39.0	34.0	41.0	15.0	41.0
47	34.09476650684946	39.0	34.0	41.0	15.0	41.0
48	34.02453233553432	38.0	34.0	41.0	15.0	41.0
49	33.91597432877851	38.0	34.0	41.0	15.0	41.0
50	33.85929699088676	38.0	34.0	41.0	15.0	41.0
51	32.980524834818475	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	11.0
10	8.0
11	8.0
12	9.0
13	14.0
14	24.0
15	25.0
16	52.0
17	104.0
18	185.0
19	360.0
20	543.0
21	850.0
22	1430.0
23	2305.0
24	3807.0
25	6860.0
26	11057.0
27	13344.0
28	12942.0
29	10723.0
30	9638.0
31	9435.0
32	10179.0
33	12877.0
34	22092.0
35	30194.0
36	28322.0
37	40840.0
38	76741.0
39	159396.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.26623450716809	20.028891965148798	21.669619732652443	25.03525379503067
2	41.49463173508953	22.807644529795105	21.994923453515582	13.702800281599782
3	28.007552385524736	20.683374633199065	37.95202900391613	13.357043977360068
4	25.803625058935737	22.366729890266505	36.6729890266508	15.156656024146967
5	21.44227312164551	31.45262858754459	33.02661634613522	14.078481944674687
6	23.35814195815186	30.995782462017402	34.007479186805284	11.638596393025454
7	70.19606492295834	5.680866500606043	21.11890701394436	3.004161562491254
8	72.02172710225646	5.265356122534656	20.323409165771416	2.389507609437468
9	65.65580670371097	8.881372863512375	21.652396504545855	3.8104239282308083
10	33.59239938943656	28.964302706845586	27.97418238106823	9.469115522649622
11	25.21502123839316	23.04855443293598	37.54943604692468	14.186988281746176
12	24.683038780250985	20.638809530473274	38.76539595124965	15.912755738026089
13	20.719543412222894	22.617112568866002	39.344526996333606	17.318817022577498
14	16.58446163418294	25.033962052922675	40.00848243984249	18.37309387305189
15	17.931963943171958	25.005543726546808	41.51056322108812	15.551929109193113
16	20.58649397509952	24.227269106480453	40.50128205404218	14.684954864377845
17	19.69088611355705	24.69940084695224	38.77831337232959	16.83139966716112
18	18.864171164440926	26.31752312756599	39.04462753692768	15.77367817106541
19	21.873714985715484	24.640196000335855	37.91758254770296	15.568506466245701
20	22.053482429077974	24.729972076841435	39.24872278999072	13.967822704089873
21	21.748416001240074	24.202080135374572	39.38220280781676	14.667301055568593
22	20.536116032887755	22.914643834407272	39.95616688446874	16.593073248236234
23	20.731814962248837	23.43779938814482	40.19384743233962	15.636538217266715
24	19.417252077013664	23.82575260124567	40.37081610113479	16.38617922060587
25	18.599364032302166	26.559724772814857	38.69542658706664	16.145484607816332
26	18.407970909967727	28.147921694593414	37.52726114073746	15.916846254701403
27	19.265687669675707	26.135387490338847	39.41729513508393	15.181629704901514
28	17.56144924852903	26.56316941843617	39.95939623973872	15.915985093296072
29	18.35802354845863	25.688014140270276	39.21578336623687	16.73817894503422
30	18.683542559673104	25.024919858166715	38.888542032211745	17.40299554994844
31	20.071304164361266	25.943348496950414	37.460521131824436	16.52482620686389
32	19.8291025191124	26.294917640676097	37.47839023098502	16.397589609226486
33	19.07020403066596	27.379981011391013	36.90162737976572	16.64818757817731
34	18.666749912269182	28.210140606128455	36.23508845204084	16.888021029561518
35	20.59984197688212	26.284798994163477	35.07897926538626	18.036379763568135
36	19.55654493432568	29.60393034065392	33.25331708608815	17.586207638932247
37	20.062907840659307	29.778530815584435	33.569363321844	16.589198021912253
38	19.53716880270577	28.886367599663288	33.17150675258187	18.404956845049075
39	21.226336899259184	27.10010355465899	32.985495889030744	18.68806365705108
40	20.394454981711085	28.072139490924435	33.83352458292877	17.699880944435716
41	18.013128405624247	29.799629270015004	33.76829160647507	18.418950717885675
42	18.958037757621817	29.711790806671416	32.93145801084633	18.39871342486044
43	21.1092189481344	27.84673049307949	32.72176520864865	18.322285350137463
44	19.62543784675202	29.635793312651103	32.368689032463635	18.370079808133237
45	19.96322840799244	28.33005733182056	32.73059211305327	18.97612214713373
46	18.80195225290588	29.250854164468908	32.788935798264326	19.158257784360877
47	20.365821364983887	27.40301707898357	34.438921050875265	17.79224050515728
48	19.414238012095012	27.814652230730974	34.08067790625828	18.690431850915736
49	18.163401070854206	28.651701116711052	34.364861170016944	18.820036642417797
50	18.065874541700662	28.210786477182452	34.31319148569719	19.410147495419697
51	19.153306106280237	26.834650551466233	33.80532154690423	20.206721795349296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	327.0
1	974.0
2	1621.0
3	13498.5
4	25376.0
5	16987.0
6	8598.0
7	8093.0
8	7588.0
9	7212.5
10	6837.0
11	6546.5
12	6256.0
13	6001.5
14	5747.0
15	5296.5
16	4846.0
17	4425.5
18	4005.0
19	3851.5
20	3698.0
21	3578.0
22	3458.0
23	3625.0
24	3792.0
25	4684.5
26	6528.5
27	7480.0
28	9070.0
29	10660.0
30	12195.5
31	13731.0
32	15228.5
33	16726.0
34	17218.5
35	17711.0
36	19210.5
37	20710.0
38	20481.5
39	20253.0
40	22507.5
41	24762.0
42	24624.5
43	24487.0
44	26038.5
45	27590.0
46	27673.5
47	27757.0
48	27075.0
49	26393.0
50	25465.0
51	24537.0
52	22394.0
53	20251.0
54	18689.5
55	17128.0
56	16323.0
57	15518.0
58	15246.5
59	14975.0
60	15046.0
61	15117.0
62	13400.5
63	11684.0
64	9629.5
65	7575.0
66	6087.0
67	4599.0
68	3792.5
69	2986.0
70	2503.5
71	2021.0
72	1535.5
73	1050.0
74	780.5
75	408.5
76	306.0
77	208.5
78	111.0
79	86.5
80	62.0
81	44.5
82	27.0
83	29.5
84	32.0
85	16.5
86	1.0
87	2.5
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	464489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.014496372729706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13511226523161	22.992631704419352
2	7.047285717949708	4.371360346738822
3	3.0189580633824553	2.8089439171859474
4	1.8896484362899164	2.3442597829219185
5	1.437765144751974	2.2295780933373646
6	1.2353458148522063	2.2988176976280346
7	1.0903319485835279	2.36712673850906
8	0.9987892168143534	2.4781555633608243
9	0.909269501842039	2.53804821000423
>10	8.082846880160147	46.95286296844377
>50	0.11354712906832289	2.2797544357498047
>100	0.03622362399725638	2.2008963643843122
>500	0.002089824461380176	0.4441977937738437
>1k	0.002089824461380176	0.8441054378766571
>5k	0.0	0.0
>10k+	6.966081537933919E-4	2.849260945666075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13188	2.8392491533706936	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1387	0.29860771729793384	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1329	0.286120876920659	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1191	0.2564108084367983	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.1618983442019079	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	738	0.15888427928325535	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	566	0.12185433885409558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.45871053996973E-4	0.0	0.0	0.019591421971241516	0.0
2	6.45871053996973E-4	0.0	0.0	0.12336137131342184	0.0
3	6.45871053996973E-4	0.0	0.0	0.1786909916058292	0.0
4	6.45871053996973E-4	0.0	0.0	0.24607687157284672	0.0
5	6.45871053996973E-4	0.0	0.0	0.430795993015981	0.0
6	6.45871053996973E-4	0.0	0.0	0.5425316853574573	0.0
7	6.45871053996973E-4	0.0	0.0	0.6478086671589639	0.0
8	6.45871053996973E-4	0.0	0.0	0.7929143639569506	0.0
9	6.45871053996973E-4	0.0	0.0	0.8521192105733397	0.0
10	6.45871053996973E-4	0.0	0.0	1.012941103018586	0.0
11	6.45871053996973E-4	0.0	0.0	1.297124366777254	0.0
12	6.45871053996973E-4	0.0	0.0	1.5188734286495482	0.0
13	6.45871053996973E-4	0.0	0.0	1.5987461489938406	0.0
14	6.45871053996973E-4	0.0	0.0	1.6364219604769974	0.0
15	6.45871053996973E-4	0.0	0.0	1.704023130795347	0.0
16	6.45871053996973E-4	0.0	0.0	1.820710501217467	0.0
17	6.45871053996973E-4	0.0	0.0	1.976150134879405	0.0
18	6.45871053996973E-4	0.0	0.0	2.1283604132713583	0.0
19	6.45871053996973E-4	0.0	0.0	2.23966552491017	0.0
20	6.45871053996973E-4	0.0	0.0	2.3514012172516465	0.0
21	6.45871053996973E-4	0.0	0.0	2.4973680754549625	0.0
22	6.45871053996973E-4	0.0	0.0	2.6547453222788913	0.0
23	6.45871053996973E-4	0.0	0.0	2.8028650839955307	0.0
24	6.45871053996973E-4	0.0	0.0	2.9333310369029193	0.0
25	6.45871053996973E-4	0.0	0.0	3.0439902774877337	0.0
26	8.611614053292974E-4	0.0	0.0	3.1552953891265454	0.0
27	8.611614053292974E-4	0.0	0.0	3.252606627928756	0.0
28	8.611614053292974E-4	0.0	0.0	3.360036513243586	0.0
29	8.611614053292974E-4	0.0	0.0	3.4836131749083403	0.0
30	8.611614053292974E-4	0.0	0.0	3.6241977743283478	0.0
31	0.0010764517566616218	0.0	0.0	3.770164632531664	0.0
32	0.0010764517566616218	0.0	0.0	3.901061166141717	0.0
33	0.0010764517566616218	0.0	0.0	4.035187055021755	0.0
34	0.0010764517566616218	0.0	0.0	4.169312943901793	0.0
35	0.0010764517566616218	0.0	0.0	4.324106706509735	0.0
36	0.0010764517566616218	0.0	0.0	4.4653371769837396	0.0
37	0.0010764517566616218	0.0	0.0	4.615825132565034	0.0
38	0.0010764517566616218	0.0	0.0	4.754687409174383	0.0
39	0.0010764517566616218	0.0	0.0	4.915509301619629	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.0289423E-4	45.0	1
CGCGGGT	25	3.8872044E-5	45.0	4
TAGCGAG	20	7.0289423E-4	45.0	1
ATAGCGG	50	2.1827873E-11	45.0	2
ACCCTAT	20	7.0289423E-4	45.0	39
CCGTCAT	20	7.0289423E-4	45.0	35
ATCGTCT	20	7.0289423E-4	45.0	40
AACTCGC	25	3.8872044E-5	45.0	40
GGTTACG	25	3.8872044E-5	45.0	1
TATCGCG	20	7.0289423E-4	45.0	32
GATAGCG	25	3.8872044E-5	45.0	1
CGTTTTT	6720	0.0	43.72768	1
CGTTATT	440	0.0	43.465908	1
CGTTGAT	50	1.0786607E-9	40.5	25
GGTCGTT	45	1.924127E-8	40.0	8
GCGAAAG	45	1.924127E-8	40.0	1
GCTACGG	45	1.924127E-8	40.0	2
CTATGCC	130	0.0	39.807693	9
GCCCTCG	130	0.0	39.807693	13
CGTGTAC	130	0.0	39.807693	18
>>END_MODULE