FastQCFastQC Report
Sat 18 Jun 2016
SRR3550877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393942
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131933.3489701529666807No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10350.2627290311771784No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10070.25562138588929334No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8260.20967553599260802No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.18479877748501047No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6020.1528143736895279No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5810.14748363972361414No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5780.14672210629991217No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4270.10839159064024655No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA4120.1045839235217367No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA4050.10280701219976544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT302.1618544E-645.0000046
GCACGTC207.027634E-445.039
CGCGCGG351.2092096E-745.02
GCGTTAG207.027634E-445.01
ACGGGCT453.8380676E-1045.05
GCACCAC453.8380676E-1045.029
CGTTAGG207.027634E-445.02
TTGCGCG207.027634E-445.01
CGGGAAT207.027634E-445.06
GCGATAT406.7957444E-945.09
ACGTCCC207.027634E-445.043
AACACGG207.027634E-445.02
CGTTATT5200.044.5673071
CGTTTTT67800.043.340711
ACATACG2700.041.66666417
CTACGAA650.041.5384611
GCTACGA650.041.5384610
CCATCGA556.002665E-1140.90909231
CATACGA2700.040.83333218
TTGAGCG501.0768417E-940.51